ATE517116T1 - Polyanion für die verbesserte nukleinsäure- verstärkung - Google Patents
Polyanion für die verbesserte nukleinsäure- verstärkungInfo
- Publication number
- ATE517116T1 ATE517116T1 AT09011394T AT09011394T ATE517116T1 AT E517116 T1 ATE517116 T1 AT E517116T1 AT 09011394 T AT09011394 T AT 09011394T AT 09011394 T AT09011394 T AT 09011394T AT E517116 T1 ATE517116 T1 AT E517116T1
- Authority
- AT
- Austria
- Prior art keywords
- polyanion
- nucleic acid
- improved nucleic
- group
- independently
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 2
- 102000039446 nucleic acids Human genes 0.000 title abstract 2
- 108020004707 nucleic acids Proteins 0.000 title abstract 2
- 229920000447 polyanionic polymer Polymers 0.000 title 1
- 230000002787 reinforcement Effects 0.000 title 1
- 150000001875 compounds Chemical class 0.000 abstract 2
- 229910019142 PO4 Inorganic materials 0.000 abstract 1
- 239000000654 additive Substances 0.000 abstract 1
- 230000000996 additive effect Effects 0.000 abstract 1
- 239000010452 phosphate Substances 0.000 abstract 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 abstract 1
- 238000003752 polymerase chain reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Macromolecular Compounds Obtained By Forming Nitrogen-Containing Linkages In General (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP08015812 | 2008-09-09 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE517116T1 true ATE517116T1 (de) | 2011-08-15 |
Family
ID=40481991
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT09011394T ATE517116T1 (de) | 2008-09-09 | 2009-09-05 | Polyanion für die verbesserte nukleinsäure- verstärkung |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US7910720B2 (de) |
| EP (1) | EP2163556B1 (de) |
| JP (1) | JP5436105B2 (de) |
| CN (1) | CN101671672B (de) |
| AT (1) | ATE517116T1 (de) |
| CA (1) | CA2678229C (de) |
| ES (1) | ES2367469T3 (de) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009055050A1 (en) * | 2007-10-25 | 2009-04-30 | Canon U.S. Life Sciences, Inc | High-resolution melting analysis |
| EP2625284B1 (de) | 2010-10-04 | 2015-01-28 | Roche Diagniostics GmbH | Verfahren für zelllyse in einem rt-pcr-reaktionspuffer |
| JP6174999B2 (ja) | 2010-10-04 | 2017-08-02 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | Ｐｃｒ反応緩衝液中での細胞溶解のための方法 |
| EP2625285B1 (de) | 2010-10-04 | 2020-04-29 | Roche Diagniostics GmbH | Verfahren für zelllyse und pcr im gleichen reaktionsgefäss |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5118801A (en) | 1988-09-30 | 1992-06-02 | The Public Health Research Institute | Nucleic acid process containing improved molecular switch |
| US5449603A (en) | 1989-10-24 | 1995-09-12 | Stratagene | Method for hybridizing nucleic acids using single-stranded nucleic acid binding protein |
| ATE137269T1 (de) | 1990-01-26 | 1996-05-15 | Abbott Lab | Verbessertes verfahren zur amplifikation von nuklein säurezielsequenz, einsetzbar für die polymerase und ligasekettenreaktion |
| DE69131891T2 (de) | 1990-02-16 | 2000-06-15 | F. Hoffmann-La Roche Ag, Basel | Verbesserungen in der spezifität und zweckmässigkeit der polymerase-kettenreaktion |
| US5693502A (en) | 1990-06-11 | 1997-12-02 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligand inhibitors to DNA polymerases |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| DE4110808A1 (de) | 1991-04-04 | 1992-10-08 | Basf Ag | Indolizintrimethinfarbstoffe |
| US5338671A (en) | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
| JP3087152B2 (ja) * | 1993-09-08 | 2000-09-11 | 富士通株式会社 | 樹脂フィルム多層回路基板の製造方法 |
| GB2293238A (en) | 1994-09-13 | 1996-03-20 | Inceltec Ltd | Primers for replication and/or amplification reactions |
| DE69534217T2 (de) | 1994-12-09 | 2006-01-12 | Wakunaga Seiyaku K.K. | Verfahren zur hemmung von nichtspezifischer hybridisierung in primerverlängerungsreaktionen |
| CA2168712A1 (en) | 1995-02-07 | 1996-08-08 | John William Henderson Sutherland | Use of exonuclease and/or glycosylase as supplements to anti-polymerase antibody to increase specificity in polymerase chain reaction |
| CA2176193A1 (en) | 1995-05-22 | 1996-11-23 | John Wesley Backus | Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers |
| JP4335975B2 (ja) | 1995-06-07 | 2009-09-30 | ギリード・サイエンシズ・インコーポレーテッド | Ｄｎａポリメラーゼに対する結合・阻害核酸リガンド |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| AU736742B2 (en) | 1996-06-03 | 2001-08-02 | Governors Of The University Of Alberta, The | Methods for detection of rearranged DNA |
| US6174670B1 (en) | 1996-06-04 | 2001-01-16 | University Of Utah Research Foundation | Monitoring amplification of DNA during PCR |
| WO1997046707A2 (en) | 1996-06-04 | 1997-12-11 | University Of Utah Research Foundation | System and method for monitoring for dna amplification by fluorescence |
| US6787305B1 (en) | 1998-03-13 | 2004-09-07 | Invitrogen Corporation | Compositions and methods for enhanced synthesis of nucleic acid molecules |
| DE19913923A1 (de) | 1999-03-26 | 2000-09-28 | Amphenol Tuchel Elect | Kontaktiereinrichtung für eine SIM-Karte |
| JP2002543795A (ja) | 1999-05-12 | 2002-12-24 | インビトロゲン・コーポレーション | 核酸合成の感度および特異性の増大のための組成物および方法 |
| EP1307592B1 (de) | 2000-08-11 | 2006-07-05 | University of Utah Research Foundation | Einfach markierte oligonukleotidsonden |
| EP1275735A1 (de) | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung |
| US6403341B1 (en) | 2001-08-02 | 2002-06-11 | Wayne M. Barnes | Magnesium precipitate hot start method for PCR |
| US6667165B2 (en) | 2001-11-13 | 2003-12-23 | Eppendorf Ag | Method and compositions for reversible inhibition of thermostable polymerases |
| WO2006119419A2 (en) | 2005-05-03 | 2006-11-09 | Geunsook Jeon | Materials and kits for use in hot-start pcr, and methods of amplifying nucleic acids in a polymerase chain reaction |
| EP1989324B1 (de) | 2006-02-27 | 2011-05-18 | Roche Diagnostics GmbH | Pcr-heissstart durch magnesium-sequestrierung |
-
2009
- 2009-08-25 US US12/547,013 patent/US7910720B2/en active Active
- 2009-09-05 AT AT09011394T patent/ATE517116T1/de not_active IP Right Cessation
- 2009-09-05 ES ES09011394T patent/ES2367469T3/es active Active
- 2009-09-05 EP EP09011394A patent/EP2163556B1/de active Active
- 2009-09-07 JP JP2009205508A patent/JP5436105B2/ja active Active
- 2009-09-08 CA CA2678229A patent/CA2678229C/en active Active
- 2009-09-08 CN CN200910171180.9A patent/CN101671672B/zh active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP2010063456A (ja) | 2010-03-25 |
| CA2678229A1 (en) | 2010-03-09 |
| EP2163556B1 (de) | 2011-07-20 |
| EP2163556A1 (de) | 2010-03-17 |
| CN101671672A (zh) | 2010-03-17 |
| JP5436105B2 (ja) | 2014-03-05 |
| US20100255547A1 (en) | 2010-10-07 |
| CN101671672B (zh) | 2015-05-13 |
| HK1142362A1 (en) | 2010-12-03 |
| CA2678229C (en) | 2017-03-07 |
| ES2367469T3 (es) | 2011-11-03 |
| US7910720B2 (en) | 2011-03-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |