ATE486084T1 - Verfahren zur herstellung von oligonucleotiden - Google Patents

Verfahren zur herstellung von oligonucleotiden

Info

Publication number
ATE486084T1
ATE486084T1 AT03768001T AT03768001T ATE486084T1 AT E486084 T1 ATE486084 T1 AT E486084T1 AT 03768001 T AT03768001 T AT 03768001T AT 03768001 T AT03768001 T AT 03768001T AT E486084 T1 ATE486084 T1 AT E486084T1
Authority
AT
Austria
Prior art keywords
oligonucleotide
activator
saccharin
tetrazole
preferred
Prior art date
Application number
AT03768001T
Other languages
English (en)
Inventor
Paul Mccormac
Original Assignee
Avecia Biotechnology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/GB2003/001795 external-priority patent/WO2003091267A1/en
Application filed by Avecia Biotechnology Inc filed Critical Avecia Biotechnology Inc
Application granted granted Critical
Publication of ATE486084T1 publication Critical patent/ATE486084T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
AT03768001T 2002-12-18 2003-12-16 Verfahren zur herstellung von oligonucleotiden ATE486084T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0229443.7A GB0229443D0 (en) 2002-12-18 2002-12-18 Process
PCT/GB2003/001795 WO2003091267A1 (en) 2002-04-26 2003-04-25 Process for preparing oligonucleotides
PCT/GB2003/005464 WO2004055036A1 (en) 2002-12-18 2003-12-16 Process for the preparation of oligonucleotides

Publications (1)

Publication Number Publication Date
ATE486084T1 true ATE486084T1 (de) 2010-11-15

Family

ID=9949885

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03768001T ATE486084T1 (de) 2002-12-18 2003-12-16 Verfahren zur herstellung von oligonucleotiden

Country Status (11)

Country Link
US (1) US7635772B2 (de)
EP (1) EP1575975B1 (de)
JP (1) JP2006512411A (de)
KR (1) KR20050085745A (de)
CN (1) CN100384864C (de)
AT (1) ATE486084T1 (de)
AU (1) AU2003292423A1 (de)
CA (1) CA2510477C (de)
DE (1) DE60334734D1 (de)
GB (1) GB0229443D0 (de)
WO (1) WO2004055036A1 (de)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR036122A1 (es) * 2001-07-03 2004-08-11 Avecia Biotechnology Inc Un complejo de sal que comprende un n-alquilimidazol y una 1,1-dioxo-1,2-dihidro-1l6-benzo [d]-isotiazol-3-ona y un metodo para sintetizar oligonucleotidos utilizando la quimica de fosforamidita
US8129517B1 (en) 2006-05-23 2012-03-06 Integrated Dna Technologies, Inc. Labeled solid supports for organic synthesis
JP5438922B2 (ja) * 2008-06-25 2014-03-12 日東電工株式会社 核酸の製造方法
WO2012059510A1 (en) * 2010-11-02 2012-05-10 Girindus America, Inc. Back pressure control during solid-phase synthesis on polymeric supports
KR102351838B1 (ko) 2013-08-05 2022-01-18 트위스트 바이오사이언스 코포레이션 드 노보 합성된 유전자 라이브러리
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
CA2998169A1 (en) 2015-09-18 2017-03-23 Twist Bioscience Corporation Oligonucleic acid variant libraries and synthesis thereof
WO2017053450A1 (en) 2015-09-22 2017-03-30 Twist Bioscience Corporation Flexible substrates for nucleic acid synthesis
CA3006867A1 (en) 2015-12-01 2017-06-08 Twist Bioscience Corporation Functionalized surfaces and preparation thereof
SG11201901563UA (en) 2016-08-22 2019-03-28 Twist Bioscience Corp De novo synthesized nucleic acid libraries
WO2018057526A2 (en) 2016-09-21 2018-03-29 Twist Bioscience Corporation Nucleic acid based data storage
CA3047128A1 (en) 2016-12-16 2018-06-21 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
CN110892485B (zh) 2017-02-22 2024-03-22 特韦斯特生物科学公司 基于核酸的数据存储
CN110913865A (zh) 2017-03-15 2020-03-24 特韦斯特生物科学公司 免疫突触的变体文库及其合成
WO2018215049A1 (en) * 2017-05-23 2018-11-29 F. Hoffmann-La Roche Ag Process for galnac oligonucleotide conjugates
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
US10696965B2 (en) 2017-06-12 2020-06-30 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
AU2019205269A1 (en) 2018-01-04 2020-07-30 Twist Bioscience Corporation DNA-based digital information storage
SG11202011467RA (en) 2018-05-18 2020-12-30 Twist Bioscience Corp Polynucleotides, reagents, and methods for nucleic acid hybridization
EP3930753A4 (de) 2019-02-26 2023-03-29 Twist Bioscience Corporation Variante nukleinsäurebibliotheken für glp1-rezeptor
SG11202109283UA (en) 2019-02-26 2021-09-29 Twist Bioscience Corp Variant nucleic acid libraries for antibody optimization
US11332738B2 (en) 2019-06-21 2022-05-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
KR20220123300A (ko) * 2020-01-08 2022-09-06 닛토덴코 가부시키가이샤 분절-타입 아미디트(segment-type amidite)를 사용한 핵산 합성 방법
KR20230119197A (ko) * 2020-12-18 2023-08-16 일라이 릴리 앤드 캄파니 진동 유동층 올리고뉴클레오티드 합성기

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001524926A (ja) * 1991-09-18 2001-12-04 アフィマックス テクノロジーズ ナームロゼ フェンノートシャップ オリゴマーの雑多ライブラリーの集合体を合成する方法
US5955600A (en) * 1996-12-27 1999-09-21 Isis Pharmaceuticals, Inc. Method for the synthesis of nucleotide or oligonucleotide phosphoramidites
US6274725B1 (en) * 1998-06-02 2001-08-14 Isis Pharmaceuticals, Inc. Activators for oligonucleotide synthesis
GB9814876D0 (en) * 1998-07-10 1998-09-09 Zeneca Ltd Polymer
EP2332954A3 (de) * 2000-12-05 2011-12-21 Avecia Biotechnology, Inc. Verfahren zur Herstellung von Phosphorothionatoligonukleotiden
AR036122A1 (es) * 2001-07-03 2004-08-11 Avecia Biotechnology Inc Un complejo de sal que comprende un n-alquilimidazol y una 1,1-dioxo-1,2-dihidro-1l6-benzo [d]-isotiazol-3-ona y un metodo para sintetizar oligonucleotidos utilizando la quimica de fosforamidita
GB0209539D0 (en) * 2002-04-26 2002-06-05 Avecia Ltd Monomer Polymer and process
AU2003269239A1 (en) * 2002-10-15 2004-05-04 Avecia Biotechnology Inc. Phosphitylation process

Also Published As

Publication number Publication date
CN100384864C (zh) 2008-04-30
AU2003292423A1 (en) 2004-07-09
EP1575975B1 (de) 2010-10-27
JP2006512411A (ja) 2006-04-13
EP1575975A1 (de) 2005-09-21
CA2510477C (en) 2012-01-24
WO2004055036A1 (en) 2004-07-01
US7635772B2 (en) 2009-12-22
DE60334734D1 (de) 2010-12-09
CN1747963A (zh) 2006-03-15
US20060149052A1 (en) 2006-07-06
CA2510477A1 (en) 2004-07-01
GB0229443D0 (en) 2003-01-22
KR20050085745A (ko) 2005-08-29

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