ATE465276T1 - Quantitativer test zum nachweis neu synthetisierter rna in einem zellfreien system sowie identifizierung von inhibitoren der rna- synthese - Google Patents

Quantitativer test zum nachweis neu synthetisierter rna in einem zellfreien system sowie identifizierung von inhibitoren der rna- synthese

Info

Publication number
ATE465276T1
ATE465276T1 AT05729155T AT05729155T ATE465276T1 AT E465276 T1 ATE465276 T1 AT E465276T1 AT 05729155 T AT05729155 T AT 05729155T AT 05729155 T AT05729155 T AT 05729155T AT E465276 T1 ATE465276 T1 AT E465276T1
Authority
AT
Austria
Prior art keywords
rna
newly synthesized
rnas
cell
free system
Prior art date
Application number
AT05729155T
Other languages
German (de)
English (en)
Inventor
Mingjun Huang
Yongnian Sun
Wengang Yang
Original Assignee
Achillion Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Achillion Pharmaceuticals Inc filed Critical Achillion Pharmaceuticals Inc
Application granted granted Critical
Publication of ATE465276T1 publication Critical patent/ATE465276T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701Specific hybridization probes
    • C12Q1/706Specific hybridization probes for hepatitis
    • C12Q1/707Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011Details
    • C12N2770/24011Flaviviridae
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011Details
    • C12N2770/24011Flaviviridae
    • C12N2770/24211Hepacivirus, e.g. hepatitis C virus, hepatitis G virus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Communicable Diseases (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
AT05729155T 2004-03-24 2005-03-23 Quantitativer test zum nachweis neu synthetisierter rna in einem zellfreien system sowie identifizierung von inhibitoren der rna- synthese ATE465276T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US55576504P 2004-03-24 2004-03-24
PCT/US2005/009959 WO2005095655A1 (en) 2004-03-24 2005-03-23 Quantitative assay for detection of newly synthesized rna in a cell-free system and identification of rna synthesis inhibitors

Publications (1)

Publication Number Publication Date
ATE465276T1 true ATE465276T1 (de) 2010-05-15

Family

ID=34963570

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05729155T ATE465276T1 (de) 2004-03-24 2005-03-23 Quantitativer test zum nachweis neu synthetisierter rna in einem zellfreien system sowie identifizierung von inhibitoren der rna- synthese

Country Status (8)

Country Link
US (1) US7985541B2 (https=)
EP (1) EP1735470B1 (https=)
JP (1) JP2007530049A (https=)
AT (1) ATE465276T1 (https=)
CA (1) CA2560699A1 (https=)
DE (1) DE602005020761D1 (https=)
ES (1) ES2345053T3 (https=)
WO (1) WO2005095655A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2901807B1 (fr) * 2006-05-30 2013-08-23 Univ Victor Segalen Bordeaux 2 Nouveau traitement d'une infection par le vhc
US20100062946A1 (en) * 2008-09-08 2010-03-11 Lis John T Genome-wide method for mapping of engaged rna polymerases quantitatively and at high resolution
CN109790143A (zh) 2016-05-10 2019-05-21 C4医药公司 用于靶蛋白降解的胺连接的c3-戊二酰亚胺降解决定子体
EP3454856B1 (en) 2016-05-10 2024-09-11 C4 Therapeutics, Inc. Heterocyclic degronimers for target protein degradation
CN109641874A (zh) 2016-05-10 2019-04-16 C4医药公司 用于靶蛋白降解的c3-碳连接的戊二酰亚胺降解决定子体
ES2990061T3 (es) 2016-05-10 2024-11-28 C4 Therapeutics Inc Degronímeros espirocíclicos para la degradación de proteínas diana
CN110769822A (zh) 2017-06-20 2020-02-07 C4医药公司 用于蛋白降解的n/o-连接的降解决定子和降解决定子体

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT281862B (de) * 1968-07-11 1970-06-10 Robugen Gmbh Verfahren zur Herstellung von neuen 2'-Desoxyuridinen
US5614403A (en) * 1994-06-01 1997-03-25 Baylor College Of Medicine In vitro replication system capable of rescuing cloned and manipulated rotavirus genes
JPH08154687A (ja) * 1994-12-12 1996-06-18 Yamanouchi Pharmaceut Co Ltd アンチセンスオリゴヌクレオチド及び抗ウイルス剤
IT1278077B1 (it) * 1995-05-25 1997-11-17 Angeletti P Ist Richerche Bio Metodologia per riprodurre in vitro le attivita' di rna polimerasi rna-dipendente e di nucleotidiltransferasi terminale codificate dal
US5770370A (en) * 1996-06-14 1998-06-23 David Sarnoff Research Center, Inc. Nuclease protection assays
US20030004122A1 (en) * 1997-11-05 2003-01-02 Leonid Beigelman Nucleotide triphosphates and their incorporation into oligonucleotides
US6004754A (en) * 1998-01-21 1999-12-21 Becton Dickinson And Company DNA sequence, related probes and primers for the detection of Streptococcus agalactiae
WO2000033635A2 (en) * 1998-12-10 2000-06-15 Eli Lilly And Company Primer-independent rna synthesis catalyzed by hcv polymerase
AU2405300A (en) * 1999-01-05 2000-07-24 Advanced Research And Technology Institute, Inc. Uses of flavivirus rna-dependent rna polymerases
DE19915178A1 (de) * 1999-04-03 2000-10-05 Univ Mainz Johannes Gutenberg Hepatitis C Virus Zellkultursystem
EP1111040A1 (en) * 1999-12-10 2001-06-27 Daniel Favre Infection of eukaryotic cells with viruses in vitro
US6447994B1 (en) * 2000-06-20 2002-09-10 The General Hospital Corporation Production of replicative hepatitis C virus

Also Published As

Publication number Publication date
EP1735470B1 (en) 2010-04-21
DE602005020761D1 (de) 2010-06-02
US20070141558A1 (en) 2007-06-21
ES2345053T3 (es) 2010-09-14
CA2560699A1 (en) 2005-10-13
JP2007530049A (ja) 2007-11-01
US7985541B2 (en) 2011-07-26
EP1735470A1 (en) 2006-12-27
WO2005095655A1 (en) 2005-10-13

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