ATE443152T1 - Verfahren und dns-konstrukte zur hemmung der genexpression in bakterien. - Google Patents

Verfahren und dns-konstrukte zur hemmung der genexpression in bakterien.

Info

Publication number
ATE443152T1
ATE443152T1 AT97954292T AT97954292T ATE443152T1 AT E443152 T1 ATE443152 T1 AT E443152T1 AT 97954292 T AT97954292 T AT 97954292T AT 97954292 T AT97954292 T AT 97954292T AT E443152 T1 ATE443152 T1 AT E443152T1
Authority
AT
Austria
Prior art keywords
cold
bacteria
shock
gene expression
dna constructs
Prior art date
Application number
AT97954292T
Other languages
English (en)
Inventor
Li Fang
Weining Jiang
Masanori Mitta
Masayori Inouye
Original Assignee
Univ New Jersey Med
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ New Jersey Med filed Critical Univ New Jersey Med
Application granted granted Critical
Publication of ATE443152T1 publication Critical patent/ATE443152T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
AT97954292T 1996-12-19 1997-12-19 Verfahren und dns-konstrukte zur hemmung der genexpression in bakterien. ATE443152T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/769,945 US5981280A (en) 1996-03-22 1996-12-19 Method and constructs for inhibiting protein expression in bacteria
PCT/US1997/024151 WO1998027220A1 (en) 1996-12-19 1997-12-19 Method and constructs for inhibiting protein expression in bacteria

Publications (1)

Publication Number Publication Date
ATE443152T1 true ATE443152T1 (de) 2009-10-15

Family

ID=25086998

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97954292T ATE443152T1 (de) 1996-12-19 1997-12-19 Verfahren und dns-konstrukte zur hemmung der genexpression in bakterien.

Country Status (8)

Country Link
US (3) US5981280A (de)
EP (1) EP0966541B1 (de)
JP (2) JP4320056B2 (de)
KR (1) KR100546823B1 (de)
AT (1) ATE443152T1 (de)
AU (1) AU5810498A (de)
DE (1) DE69739585D1 (de)
WO (1) WO1998027220A1 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5981280A (en) * 1996-03-22 1999-11-09 The University Of Medicine And Denistry Of New Jersey Method and constructs for inhibiting protein expression in bacteria
CA2309600C (en) * 1997-11-20 2009-08-25 Takara Shuzo Co., Ltd. Low-temperature inducible expression vector
AU5493599A (en) * 1998-08-20 2000-03-14 University Of Medicine And Dentistry Of New Jersey Cold-shock regulatory elements, constructs thereof, and methods of use
DE19951765A1 (de) * 1999-10-27 2001-05-03 Thomas Schweder Wirts-Vektor-Systeme zur Überproduktion von thermolabilen Enzymen psychrophiler Organismen
US7081341B2 (en) 2002-07-01 2006-07-25 Wayne State University Methods and compositions for the identification of antibiotics that are not susceptible to antibiotic resistance
TW200510534A (en) * 2002-12-11 2005-03-16 Takara Bio Inc Cold-induced expression vector
US8097416B2 (en) * 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US8546082B2 (en) * 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
CA2608093A1 (en) * 2005-05-11 2006-11-16 Wayne State University Novel targets for the identification of antibiotics that are not susceptible to antibiotic resistance
WO2006122224A1 (en) 2005-05-11 2006-11-16 Wayne State University Methods and compositions for the identification of antibiotics that are not susceptible to antibiotic resistance in pseudomonas aeruginosa
US8362203B2 (en) * 2009-02-10 2013-01-29 Wayne State University Non-natural peptides as models for the development of antibiotics
WO2019245066A1 (ko) * 2018-06-19 2019-12-26 서울대학교산학협력단 박테리아에서의 고품질 재조합 단백질 생산을 위해 전사 완료 후 번역이 개시되는 유전자 발현 카세트

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2009917C (en) * 1989-02-13 2007-05-22 Joel Goldstein Cold shock protein, gene coding therefor, promoter for gene encoding cold shock and other proteins, methods and uses as antifreeze protein in agriculture and other applications
US5714575A (en) * 1989-02-13 1998-02-03 The University Of Medicine And Dentistry Of New Jersey Nucleic acids sequence, stress-induced proteins and uses thereof
US5981280A (en) 1996-03-22 1999-11-09 The University Of Medicine And Denistry Of New Jersey Method and constructs for inhibiting protein expression in bacteria
US5654169A (en) * 1994-07-21 1997-08-05 Yissum Research & Development Company Of The Hebrew University Vectors and transformed host cells for recombinant protein production at reduced temperatures
US5726039A (en) * 1994-07-21 1998-03-10 Yissum Research Development Co. Of The Hebrew University Of Jerusalem Vectors and transformed host cells for recombinant protein production at reduced temperatures
CA2309600C (en) 1997-11-20 2009-08-25 Takara Shuzo Co., Ltd. Low-temperature inducible expression vector

Also Published As

Publication number Publication date
US5981280A (en) 1999-11-09
EP0966541A4 (de) 2005-04-20
AU5810498A (en) 1998-07-15
US7429654B1 (en) 2008-09-30
WO1998027220A1 (en) 1998-06-25
KR20000057704A (ko) 2000-09-25
US6686174B1 (en) 2004-02-03
KR100546823B1 (ko) 2006-01-26
JP4320056B2 (ja) 2009-08-26
EP0966541A1 (de) 1999-12-29
EP0966541B1 (de) 2009-09-16
JP2007061106A (ja) 2007-03-15
DE69739585D1 (de) 2009-10-29
JP2001526527A (ja) 2001-12-18

Similar Documents

Publication Publication Date Title
DE69739585D1 (de) Verfahren und dns-konstrukte zur hemmung der genexpression in bakterien.
ATE328896T1 (de) Faktor viii vom schwein und hybriden
BR9206578A (pt) Seqüência de DNA sintético tendo melhorada atividade inseticida em milho.
DE69729179D1 (de) Kohlenhydratmodifizierte luckenhafte oligonukleotide
ATE192898T1 (de) Verfahren zur inaktivierung von bakterien in blutpräparaten mit hilfe von methoxypsoralen
DE3580084D1 (de) Derivat des tryptophan-operons zur expression von verschmolzenen gen-produkten.
DE69824597D1 (de) Ikk-beta proteine, nukleinsäuren und verfahren
DE69531607D1 (de) Fusionsgene für den inhibitor des terminalen komplements und proteine
DE3585953D1 (de) Dna-fragmente, expressionsvektoren, proteine, wirte und verfahren zur herstellung der proteine.
ES2133813T3 (es) Cadenas de lamininas: diagnostico y uso terapeutico.
EP0681585A4 (de) An tata-bindende proteine assoziierte faktoren, für tafs kodierende nukleinsäuren und verfahren zu ihrer verwendung.
ES2196005T3 (es) Secuencias geneticas que codifican enzimas de la ruta flavonoide con actividad flavonoide -3`-hidroxilasa y sus usos.
EP0787207A4 (de) Neue methode zum auffindenund bewerten biologisch aktiver moleküle
DE69823372T8 (de) Verfahren und reagentiensatz zur amplifizierung, sequenzierung und typisierung von hla i genen
DE69534685D1 (de) Transferase und amylase, verfahren zur herstellung dieser enzyme, ihre verwendung und für die kodierende gene
DE68926455D1 (de) Verfahren zur identifizierung von bakterien
EP1102528A4 (de) Translations-kontrollelemente für proteinexpression mit hoher ausbeute in den plastiden von höheren pflanzen und verfahren zu deren verwendung
ATE113982T1 (de) Verzweigungsenzym und dessen verwendung.
ZA97835B (en) Purified sr-p70 protein
IL129593A0 (en) Novel constructs and vectors for the targeted and inducible expression of genes
ATE319841T1 (de) Verfahren zur expression von fremdgenen und entsprechende vektoren
IL92219A0 (en) Wounding stimulated dna sequences of solanum tuberosum and their use
WO2000011148A3 (en) Cold-shock regulatory elements, constructs thereof, and methods of use
DE59902472D1 (de) Zusammensetzung zur induktion einer tumorspezifischen immunantwort, verfahren zu deren herstellung sowie verwendung der zusammensetzung zur behandlung von neoplasien
ATE406437T1 (de) Methode zur identifikation antifungischer moleküle, welche auf die glukanosyltransferaseaktivität wirken

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties