ATE429504T1 - Proteinexpressionsvektor und benutzung desselbigen - Google Patents

Proteinexpressionsvektor und benutzung desselbigen

Info

Publication number
ATE429504T1
ATE429504T1 AT99972690T AT99972690T ATE429504T1 AT E429504 T1 ATE429504 T1 AT E429504T1 AT 99972690 T AT99972690 T AT 99972690T AT 99972690 T AT99972690 T AT 99972690T AT E429504 T1 ATE429504 T1 AT E429504T1
Authority
AT
Austria
Prior art keywords
expression vector
nucleotide sequence
protein expression
downstream side
cloning site
Prior art date
Application number
AT99972690T
Other languages
English (en)
Inventor
Hidetoshi Uemura
Akira Okui
Katsuya Kominami
Nozomi - Yamaguchi
Shinichi Mitsui
Original Assignee
Fuso Pharmaceutical Ind
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fuso Pharmaceutical Ind filed Critical Fuso Pharmaceutical Ind
Application granted granted Critical
Publication of ATE429504T1 publication Critical patent/ATE429504T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6445—Kallikreins (3.4.21.34; 3.4.21.35)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/026—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a baculovirus

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT99972690T 1998-11-20 1999-11-19 Proteinexpressionsvektor und benutzung desselbigen ATE429504T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP33151598 1998-11-20
PCT/JP1999/006474 WO2000031284A1 (en) 1998-11-20 1999-11-19 Protein expression vector and utilization thereof

Publications (1)

Publication Number Publication Date
ATE429504T1 true ATE429504T1 (de) 2009-05-15

Family

ID=18244518

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99972690T ATE429504T1 (de) 1998-11-20 1999-11-19 Proteinexpressionsvektor und benutzung desselbigen

Country Status (8)

Country Link
US (1) US7029909B1 (de)
EP (1) EP1132479B1 (de)
JP (1) JP4475814B2 (de)
AT (1) ATE429504T1 (de)
AU (1) AU765111B2 (de)
CA (1) CA2350226C (de)
DE (1) DE69940785D1 (de)
WO (1) WO2000031284A1 (de)

Families Citing this family (35)

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CZ303658B6 (cs) * 2001-02-01 2013-02-06 F. Hoffmann-La Roche Ag Zpusob rekombinantní produkce trypsinu
US7985553B2 (en) 2001-10-29 2011-07-26 Nathaniel Heintz Method for isolating cell type-specific mRNAs
PL195457B1 (pl) * 2002-08-05 2007-09-28 Zaklady Farm Polpharma Sa Kaseta ekspresyjna, dwucistronowy wektor plazmidowy, środek farmaceutyczny oraz ich zastosowanie
JP2006345831A (ja) * 2005-06-20 2006-12-28 P-Lap:Kk Ｄｎａ、ベクター、形質転換体、及びａｐａタンパク質の製造方法
JP5130662B2 (ja) * 2006-06-07 2013-01-30 Ｊｎｃ株式会社 タンパク質の可溶性タンパク質としての製造方法
WO2008051383A2 (en) * 2006-10-19 2008-05-02 Amgen Inc. Use of alcohol co-solvents to improve pegylation reaction yields
PE20081140A1 (es) * 2006-10-25 2008-09-22 Amgen Inc Agentes terapeuticos a base de peptidos derivados de toxinas
EP2162540A2 (de) 2007-05-22 2010-03-17 Amgen Inc. Zusammensetzungen und verfahren zur herstellung biologisch aktiver fusionsproteine
JP5670755B2 (ja) 2008-03-12 2015-02-18 ザ ロックフェラー ユニバーシティ 翻訳プロファイリング及び分子表現型解析のための方法及び組成物
EP2408814B1 (de) 2009-03-20 2018-11-21 Amgen Inc. Träger-immunglobuline und verwendungen davon
AU2010321832B2 (en) 2009-11-20 2014-08-14 Amgen Inc. Anti-Orai1 antigen binding proteins and uses thereof
SG187787A1 (en) 2010-08-10 2013-03-28 Amgen Inc Dual function in vitro target binding assay for the detection of neutralizing antibodies against target antibodies
WO2012040518A2 (en) 2010-09-22 2012-03-29 Amgen Inc. Carrier immunoglobulins and uses thereof
JP5940554B2 (ja) * 2010-12-09 2016-06-29 アンスティテュ・パストゥールＩｎｓｔｉｔｕｔ Ｐａｓｔｅｕｒ 高収量の組換えタンパク質発現を得るためのｍｇｍｔに基づく方法
CA2823707A1 (en) 2011-01-18 2012-07-26 Amgen Inc. Nav1.7 knockout mice and uses thereof
CA2830065A1 (en) 2011-03-16 2012-09-20 Amgen Inc. Potent and selective inhibitors of nav1.3 and nav1.7
EP2816893A1 (de) 2012-02-22 2014-12-31 Amgen Inc. Aus induzierten pluripotenten stammzellen gewonnene autologe säugetiere und entsprechende verfahren
US9540657B2 (en) 2012-05-25 2017-01-10 California Institute Of Technology Expression of secreted and cell-surface polypeptides
US10344060B2 (en) 2013-03-12 2019-07-09 Amgen Inc. Potent and selective inhibitors of Nav1.7
EP3039129B1 (de) 2013-08-30 2018-06-27 Amgen Inc. Herstellung rekombinanter aav-vektoren mit hohem titer in haftenden und hängenden zellen
WO2015192009A2 (en) * 2014-06-13 2015-12-17 Whitehead Institute For Biomedical Research Amyloid beta expression constructs and uses therefor
ES2854400T3 (es) 2014-10-13 2021-09-21 Univ Maryland Fc-ELA--32 y su uso en el tratamiento de afecciones cardiacas
MX377591B (es) 2014-10-15 2025-03-10 Amgen Inc Elementos promotores y reguladores para mejorar la expresión de genes heterólogos en células hospederas.
KR101787485B1 (ko) * 2015-12-30 2017-11-15 재단법인 의약바이오컨버젼스연구단 재조합 picp 단백질 및 이에 특이적으로 결합하는 항체의 제조방법
JP6937309B2 (ja) 2016-01-27 2021-09-22 ジャスト−エヴォテック バイオロジックス、インコーポレイテッド ハイブリッドプロモーターおよびその使用
CN109937034B (zh) 2016-11-21 2022-09-16 济世-伊沃泰克生物制品有限公司 阿柏西普制剂及其用途
DK3668985T3 (da) 2017-08-17 2021-09-20 Just Evotec Biologics Inc Fremgangsmåde til rensning af glycosyleret protein fra værtscellegalectiner og andre urenheder
CA3081094A1 (en) 2017-11-20 2019-05-23 Alison J. GILLESPIE Aflibercept formulations containing a lysine salt as tonicifying agent and uses thereof
JP2022520793A (ja) 2019-02-15 2022-04-01 ジャスト－エヴォテック バイオロジックス、インコーポレイテッド 自動バイオ製造システム、施設、及びプロセス
US20240318114A1 (en) 2021-07-15 2024-09-26 Just-Evotec Biologics, Inc. Bidirectional tangential flow filtration (tff) perfusion system
US20250043274A1 (en) 2022-02-10 2025-02-06 Amgen Inc. Antibody Protein Product Expression Constructs for High Throughput Sequencing
CA3266599A1 (en) 2022-09-07 2024-11-07 Quantitative Biosciences, Inc. UNIQUE VARIABLE DOMAIN ANTIBODIES SPECIFIC TO FENTANYL AND USE IN A CONTINUOUS AGGLUTINATION TEST
WO2025128343A1 (en) 2023-12-11 2025-06-19 Just-Evotec Biologics, Inc. Protein expression using trans-splicing and split selectable markers
CN118580307B (zh) * 2024-05-21 2025-05-27 广东合鸣生物科技有限公司 一种具备紧致抗皱功效的Pro.AH8乙酰基六肽-8及其制备方法和应用
CN121320399A (zh) * 2025-12-15 2026-01-13 江苏省中国科学院植物研究所 用于高产ghk的重组载体、重组菌及发酵方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3582965B2 (ja) * 1996-08-23 2004-10-27 株式会社イムノ・ジャパン 哺乳動物細胞用発現ベクター

Also Published As

Publication number Publication date
AU1184000A (en) 2000-06-13
WO2000031284A1 (en) 2000-06-02
JP4475814B2 (ja) 2010-06-09
AU765111B2 (en) 2003-09-11
US7029909B1 (en) 2006-04-18
CA2350226C (en) 2012-04-24
DE69940785D1 (de) 2009-06-04
EP1132479A4 (de) 2005-08-10
EP1132479A1 (de) 2001-09-12
CA2350226A1 (en) 2000-06-02
EP1132479B1 (de) 2009-04-22

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