ATE420164T1 - Methoden zur herstellung von rekombinierten nucleinsäuren - Google Patents
Methoden zur herstellung von rekombinierten nucleinsäurenInfo
- Publication number
- ATE420164T1 ATE420164T1 AT01933024T AT01933024T ATE420164T1 AT E420164 T1 ATE420164 T1 AT E420164T1 AT 01933024 T AT01933024 T AT 01933024T AT 01933024 T AT01933024 T AT 01933024T AT E420164 T1 ATE420164 T1 AT E420164T1
- Authority
- AT
- Austria
- Prior art keywords
- methods
- recombinate
- producing
- fragments
- primer
- Prior art date
Links
- 101000911390 Homo sapiens Coagulation factor VIII Proteins 0.000 title 1
- 239000002253 acid Substances 0.000 title 1
- 150000007513 acids Chemical class 0.000 title 1
- 102000057593 human F8 Human genes 0.000 title 1
- 229940047431 recombinate Drugs 0.000 title 1
- 239000012634 fragment Substances 0.000 abstract 4
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Steroid Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/566,645 US6534292B1 (en) | 2000-05-08 | 2000-05-08 | Methods for forming recombined nucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE420164T1 true ATE420164T1 (de) | 2009-01-15 |
Family
ID=24263783
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01933024T ATE420164T1 (de) | 2000-05-08 | 2001-05-03 | Methoden zur herstellung von rekombinierten nucleinsäuren |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US6534292B1 (de) |
| EP (1) | EP1280894B1 (de) |
| AT (1) | ATE420164T1 (de) |
| AU (1) | AU2001259490A1 (de) |
| CA (1) | CA2408874C (de) |
| DE (1) | DE60137321D1 (de) |
| DK (1) | DK1280894T3 (de) |
| ES (1) | ES2317903T3 (de) |
| WO (1) | WO2001085929A2 (de) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6994963B1 (en) * | 2000-07-10 | 2006-02-07 | Ambion, Inc. | Methods for recombinatorial nucleic acid synthesis |
Family Cites Families (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE93541T1 (de) | 1985-07-03 | 1993-09-15 | Genencor Int | Hybride polypeptide und verfahren zu deren herstellung. |
| IL79881A (en) | 1985-08-28 | 1995-12-08 | Pieczenik George | Populations of random coding series of oligonucleotides and peptides |
| EP0731175A3 (de) | 1989-07-11 | 2004-05-26 | Gen-Probe Incorporated | Oligonukleotid-Sonden für Nukleinsäure von HIV |
| GB9027836D0 (en) | 1990-12-21 | 1991-02-13 | Unilever Plc | Enzymes and enzymatic detergent compositions |
| US5270170A (en) | 1991-10-16 | 1993-12-14 | Affymax Technologies N.V. | Peptide library and screening method |
| WO1994012632A1 (en) | 1992-11-27 | 1994-06-09 | University College London | Improvements in nucleic acid synthesis by pcr |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US5521077A (en) | 1994-04-28 | 1996-05-28 | The Leland Stanford Junior University | Method of generating multiple protein variants and populations of protein variants prepared thereby |
| ATE216427T1 (de) | 1995-08-11 | 2002-05-15 | Novozymes As | Verfahren zur herstellung von polypeptidabkömmlingen |
| GB9521788D0 (en) | 1995-10-24 | 1996-01-03 | Univ Leeds | DNA repair |
| US5965408A (en) | 1996-07-09 | 1999-10-12 | Diversa Corporation | Method of DNA reassembly by interrupting synthesis |
| US5830696A (en) | 1996-12-05 | 1998-11-03 | Diversa Corporation | Directed evolution of thermophilic enzymes |
| US6242222B1 (en) | 1996-06-07 | 2001-06-05 | Massachusetts Institute Of Technology | Programmed sequential mutagenesis |
| JPH1066576A (ja) | 1996-08-07 | 1998-03-10 | Novo Nordisk As | 突出末端を有する２本鎖ｄｎａ及びこれを用いたｄｎａのシャフリング方法 |
| FR2753202A1 (fr) | 1996-09-06 | 1998-03-13 | Agronomique Inst Nat Rech | Bacteries multiresistantes, procedes d'obtention et utilisation |
| WO1998017684A2 (en) | 1996-10-25 | 1998-04-30 | Advanced Research & Technology Institute | Dna sequences encoding fusions of dna repair proteins and uses thereof |
| WO1998028416A1 (en) | 1996-12-20 | 1998-07-02 | Novo Nordisk A/S | In vivo recombination |
| IL131966A0 (en) | 1997-03-18 | 2001-03-19 | Novo Industri As | An in vitro method for construction of a dna library |
| JP4263248B2 (ja) | 1997-03-18 | 2009-05-13 | ノボザイムス アクティーゼルスカブ | Ｄｎａのシャッフリングによるライブラリーの作成方法 |
| DK1015575T3 (da) | 1997-03-18 | 2010-08-23 | Novozymes As | Shuffling af heterologe DNA-sekvenser |
| EP0970246A1 (de) | 1997-03-21 | 2000-01-12 | Greg Firth | Extraktion und verwendung von vntr allelen |
| US6153410A (en) | 1997-03-25 | 2000-11-28 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
| WO1998051802A1 (en) | 1997-05-12 | 1998-11-19 | Thermogen, Inc. | Method for the stabilization of proteins and the thermostabilized alcohol dehydrogenases produced thereby |
| FR2782323B1 (fr) | 1998-08-12 | 2002-01-11 | Proteus | Procede de production in vitro de sequences polynucleotidiques recombinees, banques de sequences et sequences ainsi obtenues |
-
2000
- 2000-05-08 US US09/566,645 patent/US6534292B1/en not_active Expired - Lifetime
-
2001
- 2001-05-03 CA CA2408874A patent/CA2408874C/en not_active Expired - Fee Related
- 2001-05-03 ES ES01933024T patent/ES2317903T3/es not_active Expired - Lifetime
- 2001-05-03 WO PCT/US2001/014475 patent/WO2001085929A2/en not_active Ceased
- 2001-05-03 EP EP01933024A patent/EP1280894B1/de not_active Expired - Lifetime
- 2001-05-03 DK DK01933024T patent/DK1280894T3/da active
- 2001-05-03 AU AU2001259490A patent/AU2001259490A1/en not_active Abandoned
- 2001-05-03 DE DE60137321T patent/DE60137321D1/de not_active Expired - Lifetime
- 2001-05-03 AT AT01933024T patent/ATE420164T1/de active
-
2003
- 2003-01-16 US US10/345,505 patent/US7037726B2/en not_active Expired - Fee Related
-
2006
- 2006-02-27 US US11/363,025 patent/US20060147979A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2001085929A2 (en) | 2001-11-15 |
| US20030129647A1 (en) | 2003-07-10 |
| US6534292B1 (en) | 2003-03-18 |
| EP1280894B1 (de) | 2009-01-07 |
| DE60137321D1 (de) | 2009-02-26 |
| AU2001259490A1 (en) | 2001-11-20 |
| WO2001085929A3 (en) | 2002-03-21 |
| EP1280894A2 (de) | 2003-02-05 |
| DK1280894T3 (da) | 2009-05-04 |
| CA2408874C (en) | 2012-01-03 |
| CA2408874A1 (en) | 2001-11-15 |
| ES2317903T3 (es) | 2009-05-01 |
| US20060147979A1 (en) | 2006-07-06 |
| US7037726B2 (en) | 2006-05-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
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