ATE348146T1 - Sekretion von proteinen mit mehreren disulfidbindungen in bakterien und verwendungen davon - Google Patents

Sekretion von proteinen mit mehreren disulfidbindungen in bakterien und verwendungen davon

Info

Publication number
ATE348146T1
ATE348146T1 AT03716803T AT03716803T ATE348146T1 AT E348146 T1 ATE348146 T1 AT E348146T1 AT 03716803 T AT03716803 T AT 03716803T AT 03716803 T AT03716803 T AT 03716803T AT E348146 T1 ATE348146 T1 AT E348146T1
Authority
AT
Austria
Prior art keywords
secretion
bacteria
disulfide bonds
proteins
multiple disulfide
Prior art date
Application number
AT03716803T
Other languages
English (en)
Inventor
George Georgiou
Lluis Masip
Matthew Delisa
Original Assignee
Res Dev Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Res Dev Foundation filed Critical Res Dev Foundation
Application granted granted Critical
Publication of ATE348146T1 publication Critical patent/ATE348146T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT03716803T 2002-03-23 2003-03-24 Sekretion von proteinen mit mehreren disulfidbindungen in bakterien und verwendungen davon ATE348146T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US36713002P 2002-03-23 2002-03-23

Publications (1)

Publication Number Publication Date
ATE348146T1 true ATE348146T1 (de) 2007-01-15

Family

ID=28675326

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03716803T ATE348146T1 (de) 2002-03-23 2003-03-24 Sekretion von proteinen mit mehreren disulfidbindungen in bakterien und verwendungen davon

Country Status (9)

Country Link
US (2) US20030219870A1 (de)
EP (1) EP1487966B1 (de)
JP (1) JP2005520569A (de)
CN (1) CN100516194C (de)
AT (1) ATE348146T1 (de)
AU (1) AU2003220493B2 (de)
CA (1) CA2480159A1 (de)
DE (1) DE60310385T2 (de)
WO (1) WO2003083056A2 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10256669B4 (de) * 2002-12-04 2006-03-09 Universitätsklinikum Charité an der Humboldt-Universität zu Berlin Technologietransferstelle Gemisch mindestens zweier Fusionsproteine sowie ihre Herstellung und Verwendung
CA2551644C (en) * 2003-12-24 2014-03-04 Cerus Corporation Recombinant nucleic acid molecules encoding fusion proteins comprising antigens and bacterial secretory signal polypeptides, expression cassettes, and bacteria, and methods of usethereof
US7842289B2 (en) 2003-12-24 2010-11-30 Aduro Biotech Recombinant nucleic acid molecules, expression cassettes, and bacteria, and methods of use thereof
EP1748077B1 (de) * 2004-04-20 2013-02-27 Ajinomoto Co., Inc. Verfahren zur proteinherstellung
MX2007011801A (es) * 2005-03-24 2008-02-19 Neose Technologies Inc Expresion de glicosiltransferasas eucarioticas activas, solubles en organismos procarioticos.
US20070026012A1 (en) 2005-08-01 2007-02-01 Cornell Research Foundation, Inc. Compositions and methods for monitoring and altering protein folding and solubility
AU2006283130B2 (en) * 2005-08-22 2012-02-09 Cornell Research Foundation, Inc. Compositions and methods for analyzing protein interactions
KR100742218B1 (ko) 2005-10-21 2007-07-24 학교법인 포항공과대학교 트윈 아르기닌 트랜스로케이션 분비경로를 가지는오가노포스포러스 하이드롤라제 분비서열을 이용한대장균에서의 목적 외래 단백질 분비
US7935804B2 (en) 2006-03-01 2011-05-03 Aduro Biotech Engineered Listeria and methods of use thereof
CA2671264C (en) 2006-11-30 2015-11-24 Research Development Foundation Improved immunoglobulin libraries
WO2008089132A2 (en) * 2007-01-12 2008-07-24 Cornell Research Foundation, Inc. Genetic selection for protein folding and solubility in the bacterial periplasm
US8629245B2 (en) 2007-05-01 2014-01-14 Research Development Foundation Immunoglobulin Fc libraries
US20090053786A1 (en) 2007-07-09 2009-02-26 Yung-Hsiang Kao Prevention of disulfide bond reduction during recombinant production of polypeptides
WO2011008517A2 (en) * 2009-06-30 2011-01-20 Research Development Foundation Immunoglobulin fc polypeptides
US8871477B2 (en) 2010-02-23 2014-10-28 Toray Industries, Inc. Process for production of cadaverine
CA2793424A1 (en) * 2010-03-18 2011-09-22 Cornell University Engineering correctly folded antibodies using inner membrane display of twin-arginine translocation intermediates
JP6228014B2 (ja) 2011-02-07 2017-11-08 リサーチ ディベロップメント ファウンデーション 操作された免疫グロブリンＦｃポリペプチド
GB201419899D0 (en) * 2014-11-07 2014-12-24 Univ Kent Canterbury Biopharmaceutical production method
WO2016130516A1 (en) 2015-02-09 2016-08-18 Research Development Foundation Engineered immunoglobulin fc polypeptides displaying improved complement activation
WO2017040380A2 (en) 2015-08-28 2017-03-09 Research Development Foundation Engineered antibody fc variants
US11059892B2 (en) 2017-08-11 2021-07-13 Research Development Foundation Engineered antibody Fc variants for enhanced serum half life
CN113166717B (zh) * 2018-11-08 2024-02-20 苏特罗生物制药公司 具有氧化细胞质的大肠杆菌菌株
CN110616227A (zh) * 2019-09-30 2019-12-27 天津科技大学 一种来源于黄粉虫的抗冻蛋白的基因、重组表达载体、工程菌株和应用
WO2023239940A1 (en) 2022-06-10 2023-12-14 Research Development Foundation Engineered fcriib selective igg1 fc variants and uses thereof
CN115851773A (zh) * 2022-11-14 2023-03-28 无锡新晨宇生物工程有限公司 一种芳香族酰胺生物传感器的创制与应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19513676A1 (de) * 1995-04-11 1996-10-17 Behringwerke Ag Cytoplasmatische Expression von Antikörpern, Antikörperfragmenten und Antikörperfragmentfusionsmolekülen in E.coli
US6277375B1 (en) * 1997-03-03 2001-08-21 Board Of Regents, The University Of Texas System Immunoglobulin-like domains with increased half-lives
US6022952A (en) * 1998-04-01 2000-02-08 University Of Alberta Compositions and methods for protein secretion
US7316924B2 (en) * 2000-09-18 2008-01-08 Genencor International, Inc. Twin-arginine translocation in Bacillus
AU2002243205A1 (en) * 2000-10-10 2002-07-24 Genencor International, Inc. Enhanced secretion of a polypeptide by a microorganism
US7419783B2 (en) * 2001-11-05 2008-09-02 Research Development Foundation Engineering of leader peptides for the secretion of recombinant proteins in bacteria

Also Published As

Publication number Publication date
JP2005520569A (ja) 2005-07-14
EP1487966B1 (de) 2006-12-13
WO2003083056A2 (en) 2003-10-09
US20030219870A1 (en) 2003-11-27
AU2003220493B2 (en) 2008-06-05
CA2480159A1 (en) 2003-10-09
WO2003083056A3 (en) 2004-07-15
CN100516194C (zh) 2009-07-22
EP1487966A2 (de) 2004-12-22
DE60310385D1 (de) 2007-01-25
DE60310385T2 (de) 2007-09-20
CN1656213A (zh) 2005-08-17
EP1487966A4 (de) 2005-11-23
US20110046011A1 (en) 2011-02-24
AU2003220493A1 (en) 2003-10-13

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