ATE344836T1 - Methode für die erweiterung der chemischen zusammensetzung von proteinen produziert in vivo unter verwendung von mutierten aminoacyl-trna- synthetasen ohne korrekturfunktion - Google Patents

Methode für die erweiterung der chemischen zusammensetzung von proteinen produziert in vivo unter verwendung von mutierten aminoacyl-trna- synthetasen ohne korrekturfunktion

Info

Publication number
ATE344836T1
ATE344836T1 AT02290998T AT02290998T ATE344836T1 AT E344836 T1 ATE344836 T1 AT E344836T1 AT 02290998 T AT02290998 T AT 02290998T AT 02290998 T AT02290998 T AT 02290998T AT E344836 T1 ATE344836 T1 AT E344836T1
Authority
AT
Austria
Prior art keywords
vivo
trna synthetases
chemical composition
expansion
proteins produced
Prior art date
Application number
AT02290998T
Other languages
English (en)
Inventor
Volker Doring
Leslie A Nangle
Tamara L Hendrickson
Crecy-Lagard Valerie De
Paul Schimmel
Philippe Marliere
Original Assignee
Pasteur Institut
Evologic Gmbh
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pasteur Institut, Evologic Gmbh, Scripps Research Inst filed Critical Pasteur Institut
Application granted granted Critical
Publication of ATE344836T1 publication Critical patent/ATE344836T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT02290998T 2001-04-19 2002-04-19 Methode für die erweiterung der chemischen zusammensetzung von proteinen produziert in vivo unter verwendung von mutierten aminoacyl-trna- synthetasen ohne korrekturfunktion ATE344836T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US28549501P 2001-04-19 2001-04-19

Publications (1)

Publication Number Publication Date
ATE344836T1 true ATE344836T1 (de) 2006-11-15

Family

ID=37401359

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02290998T ATE344836T1 (de) 2001-04-19 2002-04-19 Methode für die erweiterung der chemischen zusammensetzung von proteinen produziert in vivo unter verwendung von mutierten aminoacyl-trna- synthetasen ohne korrekturfunktion

Country Status (3)

Country Link
US (2) US20030148422A1 (de)
AT (1) ATE344836T1 (de)
DE (1) DE60215881T2 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030148422A1 (en) * 2001-04-19 2003-08-07 Volker Doring Method for diversifying the chemical composition of proteins produced in vivo by genetically disabling the editing function of their aminoacyl tRNA synthetases
WO2006055836A2 (en) * 2004-11-16 2006-05-26 President And Fellows Of Harvard College In vivo alteration of cellular dna
US10501734B2 (en) 2014-02-06 2019-12-10 Yale University Compositions and methods of use thereof for making polypeptides with many instances of nonstandard amino acids
WO2016073079A2 (en) 2014-09-26 2016-05-12 Yale University Compositions and methods for biocontainment of microorganisms
US11149280B2 (en) 2019-10-29 2021-10-19 Yale University Engineering organisms resistant to viruses and horizontally transferred genetic elements

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE9115660U1 (de) * 1991-12-18 1992-07-30 Aventis Research & Technologies GmbH & Co KG, 65929 Frankfurt L-Phenylalanyl-tRNA-Synthetase mit erweiterter Substratselektivität aus Mikroorganismen
FR2785291B1 (fr) 1998-10-28 2003-03-07 Pasteur Institut Procede de production in vivo de proteines chimiquement diversifiees par incorporation d'acides amines non conventionnels
FR2808284B1 (fr) 2000-04-28 2004-11-19 Pasteur Institut Souches mutantes capables de produire des proteines chimiquement diversifiees par incorporation d'acides amines non conventionnels
US20030148422A1 (en) * 2001-04-19 2003-08-07 Volker Doring Method for diversifying the chemical composition of proteins produced in vivo by genetically disabling the editing function of their aminoacyl tRNA synthetases

Also Published As

Publication number Publication date
DE60215881D1 (de) 2006-12-21
US20030148422A1 (en) 2003-08-07
DE60215881T2 (de) 2007-06-14
US20050170460A1 (en) 2005-08-04
US7524646B2 (en) 2009-04-28

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