ATE299889T1 - Neues verfahren zur trennung von proteinen unter verwendung eines ca++ enthaltenden elutionsmittels - Google Patents
Neues verfahren zur trennung von proteinen unter verwendung eines ca++ enthaltenden elutionsmittelsInfo
- Publication number
- ATE299889T1 ATE299889T1 AT99913118T AT99913118T ATE299889T1 AT E299889 T1 ATE299889 T1 AT E299889T1 AT 99913118 T AT99913118 T AT 99913118T AT 99913118 T AT99913118 T AT 99913118T AT E299889 T1 ATE299889 T1 AT E299889T1
- Authority
- AT
- Austria
- Prior art keywords
- agent containing
- new method
- proteins
- elution agent
- separating proteins
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 102000004169 proteins and genes Human genes 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 title abstract 2
- 238000010828 elution Methods 0.000 title 1
- 102000035122 glycosylated proteins Human genes 0.000 abstract 5
- 108091005608 glycosylated proteins Proteins 0.000 abstract 5
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 2
- 102000004877 Insulin Human genes 0.000 abstract 1
- 108090001061 Insulin Proteins 0.000 abstract 1
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 229940125396 insulin Drugs 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK52898 | 1998-04-15 | ||
| PCT/DK1999/000208 WO1999052934A1 (en) | 1998-04-15 | 1999-04-13 | NOVEL PROCESS FOR THE SEPARATION OF PROTEINS USING A Ca++ CONTAINING ELUANT |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE299889T1 true ATE299889T1 (de) | 2005-08-15 |
Family
ID=8094571
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT99913118T ATE299889T1 (de) | 1998-04-15 | 1999-04-13 | Neues verfahren zur trennung von proteinen unter verwendung eines ca++ enthaltenden elutionsmittels |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US6180757B1 (de) |
| EP (1) | EP1071703B1 (de) |
| JP (1) | JP2002511484A (de) |
| AT (1) | ATE299889T1 (de) |
| AU (1) | AU3137699A (de) |
| DE (1) | DE69926226T2 (de) |
| ES (1) | ES2245824T3 (de) |
| WO (1) | WO1999052934A1 (de) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007532096A (ja) | 2003-11-14 | 2007-11-15 | ノボ ノルディスク アクティーゼルスカブ | アシル化されたインスリンの製造方法 |
| JP5401097B2 (ja) * | 2005-12-23 | 2014-01-29 | ノボ・ノルデイスク・エー／エス | 分取逆相クロマトグラフィー（ｒｐｃ）を使用するタンパク質精製 |
| EP2242767A4 (de) * | 2008-02-19 | 2011-04-06 | Biocon Ltd | Verfahren zur gewinnung von in hefe exprimierten gereinigten heterologen insulinen |
| JP2012516339A (ja) | 2009-01-28 | 2012-07-19 | スマートセルズ・インコーポレイテツド | 外因的刺激型制御放出物質体およびその使用 |
| CA2750252A1 (en) | 2009-01-28 | 2010-08-05 | Smartcells, Inc. | Synthetic conjugates and uses thereof |
| MX2011007930A (es) | 2009-01-28 | 2011-09-06 | Smartcells Inc | Conjugados de insulina cristalina. |
| NZ594248A (en) | 2009-01-28 | 2015-03-27 | Smartcells Inc | Conjugate based systems for controlled drug delivery |
| US8623345B2 (en) | 2009-03-20 | 2014-01-07 | Smartcells | Terminally-functionalized conjugates and uses thereof |
| EP2408470A4 (de) | 2009-03-20 | 2012-08-29 | Smartcells Inc | Lösliche nichtdepot-insulinkonjugate und ihre verwendung |
| AU2011282988A1 (en) | 2010-07-28 | 2013-01-31 | Smartcells, Inc. | Recombinantly expressed insulin polypeptides and uses thereof |
| CA2805739A1 (en) | 2010-07-28 | 2012-02-02 | Smartcells, Inc. | Drug-ligand conjugates, synthesis thereof, and intermediates thereto |
| US9068013B2 (en) | 2010-07-28 | 2015-06-30 | Smart Cells, Inc. | Recombinant lectins, binding-site modified lectins and uses thereof |
| AU2012213432B2 (en) | 2011-02-01 | 2016-10-13 | Novo Nordisk A/S | Purification of insulin |
| CN103509082B (zh) * | 2012-06-15 | 2016-03-02 | 郭怀祖 | 一种糖基化蛋白和非糖基化蛋白的分离方法 |
| US9707276B2 (en) | 2012-12-03 | 2017-07-18 | Merck Sharp & Dohme Corp. | O-glycosylated carboxy terminal portion (CTP) peptide-based insulin and insulin analogues |
| US9738683B2 (en) | 2013-07-25 | 2017-08-22 | Bio-Rad Laboratories, Inc. | Enhanced functionality and delivery of a protein from a porous substrate |
| BR112016007176A2 (pt) | 2013-10-04 | 2018-01-23 | Merck Sharp & Dohme | conjugado, composição, usos de um conjugado e de uma composição, e, método para tratar um indivíduo que tem diabete |
| US9671402B2 (en) | 2014-02-27 | 2017-06-06 | Bio-Rad Laboratories, Inc. | Lateral flow blotting assay |
| CN107903301A (zh) * | 2017-12-22 | 2018-04-13 | 广州誉嘉生物科技有限公司 | 一种糖基化蛋白的分离方法 |
| WO2020039339A1 (en) * | 2018-08-20 | 2020-02-27 | Biocon Biologics India Limited | Chromatography process for purification of insulin analogues |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3649456A (en) | 1969-09-08 | 1972-03-14 | Rohm & Haas | Separation of polypeptide substances with macroreticular resins |
| US4269605A (en) * | 1978-06-28 | 1981-05-26 | Amicon Corporation | Method and kit for separation of glycoproteins |
| DE4406515C1 (de) * | 1994-02-28 | 1995-10-19 | Immuno Ag | Verfahren zur Isolierung und Reinigung Vitamin K-abhängiger Proteine |
-
1999
- 1999-04-13 DE DE69926226T patent/DE69926226T2/de not_active Expired - Lifetime
- 1999-04-13 WO PCT/DK1999/000208 patent/WO1999052934A1/en not_active Ceased
- 1999-04-13 JP JP2000543490A patent/JP2002511484A/ja active Pending
- 1999-04-13 EP EP99913118A patent/EP1071703B1/de not_active Expired - Lifetime
- 1999-04-13 ES ES99913118T patent/ES2245824T3/es not_active Expired - Lifetime
- 1999-04-13 AU AU31376/99A patent/AU3137699A/en not_active Abandoned
- 1999-04-13 AT AT99913118T patent/ATE299889T1/de not_active IP Right Cessation
- 1999-04-13 US US09/290,856 patent/US6180757B1/en not_active Expired - Lifetime
-
2003
- 2003-11-21 US US10/719,552 patent/US20040077527A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| ES2245824T3 (es) | 2006-01-16 |
| DE69926226T2 (de) | 2006-05-24 |
| US6180757B1 (en) | 2001-01-30 |
| EP1071703B1 (de) | 2005-07-20 |
| EP1071703A1 (de) | 2001-01-31 |
| AU3137699A (en) | 1999-11-01 |
| JP2002511484A (ja) | 2002-04-16 |
| US20040077527A1 (en) | 2004-04-22 |
| WO1999052934A1 (en) | 1999-10-21 |
| DE69926226D1 (de) | 2005-08-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |