ATE231922T1 - Modifizierung von oligonukleotid-primer-sequenzen zum manipilieren der zugabe von nicht-template- nukleotiden - Google Patents
Modifizierung von oligonukleotid-primer-sequenzen zum manipilieren der zugabe von nicht-template- nukleotidenInfo
- Publication number
- ATE231922T1 ATE231922T1 AT96936547T AT96936547T ATE231922T1 AT E231922 T1 ATE231922 T1 AT E231922T1 AT 96936547 T AT96936547 T AT 96936547T AT 96936547 T AT96936547 T AT 96936547T AT E231922 T1 ATE231922 T1 AT E231922T1
- Authority
- AT
- Austria
- Prior art keywords
- manipilate
- modification
- add
- oligonucleotide primer
- primer sequences
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US576195P | 1995-10-20 | 1995-10-20 | |
| PCT/US1996/016544 WO1997016566A1 (en) | 1995-10-20 | 1996-10-15 | Sequence modification of oligonucleotide primers to manipulate non-templated nucleotide addition |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE231922T1 true ATE231922T1 (de) | 2003-02-15 |
Family
ID=21717605
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT96936547T ATE231922T1 (de) | 1995-10-20 | 1996-10-15 | Modifizierung von oligonukleotid-primer-sequenzen zum manipilieren der zugabe von nicht-template- nukleotiden |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP0857220B1 (de) |
| AT (1) | ATE231922T1 (de) |
| AU (1) | AU710779B2 (de) |
| CA (1) | CA2235327C (de) |
| DE (1) | DE69626032T2 (de) |
| DK (1) | DK0857220T3 (de) |
| WO (1) | WO1997016566A1 (de) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6043070A (en) * | 1997-08-29 | 2000-03-28 | The Perkin-Elmer Corporation | Phosphoramidate-phosphodiester oligonucleotide chimera as primers |
| US7205129B1 (en) | 2000-02-28 | 2007-04-17 | Qiagen Gmbh | Method for reducing artifacts in nucleic acid amplification |
| US6617137B2 (en) | 2001-10-15 | 2003-09-09 | Molecular Staging Inc. | Method of amplifying whole genomes without subjecting the genome to denaturing conditions |
| US9487823B2 (en) | 2002-12-20 | 2016-11-08 | Qiagen Gmbh | Nucleic acid amplification |
| CN102994535B (zh) * | 2012-11-06 | 2014-07-23 | 河北大学 | 一种基因定向克隆用tc载体及其制备和使用方法 |
-
1996
- 1996-10-15 DE DE69626032T patent/DE69626032T2/de not_active Expired - Lifetime
- 1996-10-15 DK DK96936547T patent/DK0857220T3/da active
- 1996-10-15 WO PCT/US1996/016544 patent/WO1997016566A1/en active IP Right Grant
- 1996-10-15 EP EP96936547A patent/EP0857220B1/de not_active Expired - Lifetime
- 1996-10-15 AT AT96936547T patent/ATE231922T1/de active
- 1996-10-15 AU AU74450/96A patent/AU710779B2/en not_active Ceased
- 1996-10-15 CA CA002235327A patent/CA2235327C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| AU7445096A (en) | 1997-05-22 |
| WO1997016566A1 (en) | 1997-05-09 |
| AU710779B2 (en) | 1999-09-30 |
| CA2235327C (en) | 2007-12-18 |
| DE69626032D1 (de) | 2003-03-06 |
| DE69626032T2 (de) | 2004-01-22 |
| EP0857220B1 (de) | 2003-01-29 |
| DK0857220T3 (da) | 2003-06-02 |
| EP0857220A1 (de) | 1998-08-12 |
| CA2235327A1 (en) | 1997-05-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
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