ATE140029T1 - Herstellung von biologischaktivem insulinähnlichem wachstumsfaktor i aus hochexpressions-wirtszellsystemen - Google Patents

Herstellung von biologischaktivem insulinähnlichem wachstumsfaktor i aus hochexpressions-wirtszellsystemen

Info

Publication number
ATE140029T1
ATE140029T1 AT90914315T AT90914315T ATE140029T1 AT E140029 T1 ATE140029 T1 AT E140029T1 AT 90914315 T AT90914315 T AT 90914315T AT 90914315 T AT90914315 T AT 90914315T AT E140029 T1 ATE140029 T1 AT E140029T1
Authority
AT
Austria
Prior art keywords
igf
production
host cell
growth factor
biologically active
Prior art date
Application number
AT90914315T
Other languages
English (en)
Inventor
James A Miller
Philip K Hsieh
Larry B Tsai
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc filed Critical Amgen Inc
Application granted granted Critical
Publication of ATE140029T1 publication Critical patent/ATE140029T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT90914315T 1989-08-25 1990-08-21 Herstellung von biologischaktivem insulinähnlichem wachstumsfaktor i aus hochexpressions-wirtszellsystemen ATE140029T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/398,699 US5158875A (en) 1989-08-25 1989-08-25 Production of biologically active insulin-like growth factor i from high expression host cell systems

Publications (1)

Publication Number Publication Date
ATE140029T1 true ATE140029T1 (de) 1996-07-15

Family

ID=23576440

Family Applications (1)

Application Number Title Priority Date Filing Date
AT90914315T ATE140029T1 (de) 1989-08-25 1990-08-21 Herstellung von biologischaktivem insulinähnlichem wachstumsfaktor i aus hochexpressions-wirtszellsystemen

Country Status (10)

Country Link
US (1) US5158875A (de)
EP (1) EP0441955B1 (de)
JP (1) JPH04501514A (de)
AT (1) ATE140029T1 (de)
AU (1) AU644105B2 (de)
CA (1) CA2023969A1 (de)
DE (1) DE69027666T2 (de)
DK (1) DK0441955T3 (de)
ES (1) ES2089029T3 (de)
WO (1) WO1991002807A1 (de)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5316932A (en) * 1989-03-24 1994-05-31 Duke University Homogeneous denatured human 06-guanine alkyltransferase prepared by immunoaffinity chromatography using monoclonal antibody specific for enzyme
AU8417091A (en) * 1990-08-20 1992-03-17 Novo Nordisk A/S Biologically active compound, a process for the preparation thereof and use of the same
US5646016A (en) * 1991-02-06 1997-07-08 Genetics Institute, Inc. Peptide and protein fusions to thioredoxin, thioredoxin-like molecules, and modified thioredoxin-like molecules
US5378613A (en) * 1991-09-24 1995-01-03 Eli Lilly And Company Method for increased expression of low molecular weight recombinant polypeptides
US5288931A (en) * 1991-12-06 1994-02-22 Genentech, Inc. Method for refolding insoluble, misfolded insulin-like growth factor-I into an active conformation
US5958764A (en) * 1992-04-30 1999-09-28 Baylor College Of Medicine Specific expression vectors and methods of use
EP0695359B1 (de) * 1993-03-29 1997-06-04 E.I. Du Pont De Nemours & Company Incorporated Ein verfahren zur erhöhung der produktion von biologisch aktiven rekombinanten proteinen
US5407810A (en) * 1993-08-20 1995-04-18 Genentech, Inc. Aqueous multiple-phase isolation of polypeptide
US5663304A (en) * 1993-08-20 1997-09-02 Genentech, Inc. Refolding of misfolded insulin-like growth factor-I
SE9303784D0 (sv) * 1993-11-16 1993-11-16 Kabi Pharmacia Ab Igf
US5451660A (en) * 1993-12-13 1995-09-19 Genentech, Inc. Method for purifying polypeptides
WO1995016708A1 (en) * 1993-12-17 1995-06-22 Novo Nordisk A/S Proinsulin-like compounds
JPH10504971A (ja) * 1994-09-08 1998-05-19 カイロン コーポレイション インスリン様増殖因子の改善された生産方法
US5712249A (en) * 1994-09-08 1998-01-27 Ciba-Geigy Corporation Use of insulin-like growth factors I and II for inhibition of inflammatory response
US5789547A (en) * 1995-06-07 1998-08-04 Celtrix Pharmaceuticals, Inc. Method of producing insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-3 (IGFBP-3) with correct folding and disulfide bonding
CA2396320A1 (en) * 2000-01-11 2001-07-19 Maxygen, Inc. Integrated systems and methods for diversity generation and screening
US20080119433A1 (en) * 2006-07-06 2008-05-22 Aaron Thomas Tabor Compositions and Methods for Genetic Modification of Cells Having Cosmetic Function to Enhance Cosmetic Appearance
CL2007002502A1 (es) * 2006-08-31 2008-05-30 Hoffmann La Roche Variantes del factor de crecimiento similar a insulina-1 humano (igf-1) pegilados en lisina; metodo de produccion; proteina de fusion que la comprende; y su uso para tratar la enfermedad de alzheimer.
MX2009001691A (es) * 2006-08-31 2009-02-25 Hoffmann La Roche Metodo para produccion del factor de crecimiento i tipo insulina.
BRPI0910338A2 (pt) * 2008-04-03 2020-08-18 F. Hoffmann-La Roche Ag uso de variantes peguiladas de igf-i para o tratamento de distúbios neuromusculares
WO2009121551A1 (en) * 2008-04-03 2009-10-08 F. Hoffmann-La Roche Ag Pegylated insulin-like-growth-factor assay
EP2454368A4 (de) 2009-07-17 2013-01-09 Aaron Thomas Tabor Zusammensetzungen und verfahren zur genetischen modifizierung von zellen mit kosmetischer funktion zur verbesserung des kosmetischen erscheinungsbilds
US20110152188A1 (en) * 2009-12-23 2011-06-23 Hanns-Christian Mahler Pharmaceutical compositions of igf/i proteins
US9675671B2 (en) * 2014-01-12 2017-06-13 Igf Oncology, Llc Fusion proteins containing insulin-like growth factor-1 and epidermal growth factor and variants thereof and uses thereof
CN112892220B (zh) * 2021-01-19 2023-02-17 南开大学 一种高效的活性蛋白分离方法与装置
CN114292800B (zh) * 2021-12-27 2024-08-20 武汉佰乐博生物技术有限公司 用于igf-1基因重组表达的重组细胞和重组表达方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4532207A (en) * 1982-03-19 1985-07-30 G. D. Searle & Co. Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase
FR2594846B1 (fr) * 1986-02-21 1989-10-20 Genetica Procede de preparation de la serum albumine humaine mature
AU1987088A (en) * 1987-06-24 1989-01-19 Novo Nordisk A/S A process for preparing a protein or polypeptide, a dna sequence coding for the polypeptide, a microorganism containing the dna sequence as well as the polypeptide and its use as a pharmaceutical preparation

Also Published As

Publication number Publication date
CA2023969A1 (en) 1991-02-26
EP0441955A4 (en) 1992-07-15
DK0441955T3 (da) 1996-10-07
JPH04501514A (ja) 1992-03-19
EP0441955B1 (de) 1996-07-03
DE69027666T2 (de) 1996-11-07
WO1991002807A1 (en) 1991-03-07
AU6425490A (en) 1991-04-03
ES2089029T3 (es) 1996-10-01
EP0441955A1 (de) 1991-08-21
AU644105B2 (en) 1993-12-02
US5158875A (en) 1992-10-27
DE69027666D1 (de) 1996-08-08

Similar Documents

Publication Publication Date Title
ATE140029T1 (de) Herstellung von biologischaktivem insulinähnlichem wachstumsfaktor i aus hochexpressions-wirtszellsystemen
VAN WYK et al. Evidence from radioligand assays that somatomedin-C and insulin-like growth factor-I are similar to each other and different from other somatomedins
ATE123949T1 (de) B-ketten-analoge des von blutplättchen abstammenden wachstumsfaktors und verfahren zu deren homogener herstellung.
EP0722461A4 (de) Expression eines fusionspolypeptides, das ohne leadersequenz aus dem cytoplasma transportiert wird
SE465222C5 (sv) Ett rekombinant, humant faktor VIII-derivat och förfarande för dess framställning
CA2089094A1 (en) Expression of recombinant polypeptides with improved purification
DE69016655D1 (de) Biologisch aktive polypeptide, die auf transformierten wachstumsfaktor-beta-sequenzen basieren.
PT714406E (pt) Re-enrolamento de polipeptidos tais como o factor de crescimento semelhante a insulina igf-i recombinante
PT955313E (pt) Nova proteina e processo para a sua producao
ES8801851A1 (es) Un metodo para producir bacterianamente un polipeptido heteralogo que tiene actividad somatotropinica.
DE59611434D1 (de) Herstellung von Proteinen aus Pro-Proteinen durch Fusionsproteine abgeleitet von Furin oder Furinanalogen
DE69126487D1 (de) cDNA für Ratten C-Typ natriuretisches Peptid und Vorläuferprotein
ATE108458T1 (de) Immunglobulin-e-kompetitor.
KR930013115A (ko) Dna 분자 및 숙주
ES2091237T3 (es) Nuevo procedimiento para la produccion de proteina no fusionada en e. coli.
DE3881023D1 (de) Proteine, expressionsvektoren, die fuer solche proteine kodierende dna enthalten und zellen, die solche expressionsvektoren enthalten.
DK0467839T3 (da) In-vitro-behandling af fusionsproteiner
NO874704L (no) Minaktivin fremstilt ved rekombinant dna-teknikk.

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee