ES2089029T3 - Produccion de factor de crecimiento similar a la insulina i biologicamente activo a partir de sistemas de celulas hospedantes de expresion elevada. - Google Patents

Produccion de factor de crecimiento similar a la insulina i biologicamente activo a partir de sistemas de celulas hospedantes de expresion elevada.

Info

Publication number
ES2089029T3
ES2089029T3 ES90914315T ES90914315T ES2089029T3 ES 2089029 T3 ES2089029 T3 ES 2089029T3 ES 90914315 T ES90914315 T ES 90914315T ES 90914315 T ES90914315 T ES 90914315T ES 2089029 T3 ES2089029 T3 ES 2089029T3
Authority
ES
Spain
Prior art keywords
igf
insulin
expression
production
growth factor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90914315T
Other languages
English (en)
Inventor
James A Miller
Philip K Hsieh
Larry B Tsai
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amgen Inc
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc filed Critical Amgen Inc
Application granted granted Critical
Publication of ES2089029T3 publication Critical patent/ES2089029T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

SE PRESENTA UN NUEVO METODO PARA REDOBLAR IGF-I REDUCIDO. EL METODO SE LLEVA A CABO SUMINISTRANDO UNA CARGA POSITIVA ADICIONAL AL EXTREMO TERMINAL AMINO DE LA MOLECULA IGF-I MEDIANTE LA PRESENCIA DE UNA SECUENCIA CORTA DE ENCABEZAMIENTO. SE HA DESCUBIERTO QUE LA CARGA POSITIVA ADICIONAL EN EL EXTREMO DE ENCUENTRO DE LA MOLECULA IGF-I HACE QUE EL IGF-I RECOMBINANTE SE REDOBLE SIMPLEMENTE REMOVIENDO LA PROTEINA DEL CUERPO DE INCLUSION SOLUBILIZADO ENTRE 2 Y 16 HORAS, O DURANTE TODA LA NOCHE. LA PRESENTE INVENCION TAMBIEN SUMINISTRA UNA NUEVA PROTEINA DE FUSION INTERMEDIA DE IGF-I LIGADA A LA SECUENCIA DE ENCABEZAMIENTO POSITIVAMENTE CARGADA.
ES90914315T 1989-08-25 1990-08-21 Produccion de factor de crecimiento similar a la insulina i biologicamente activo a partir de sistemas de celulas hospedantes de expresion elevada. Expired - Lifetime ES2089029T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/398,699 US5158875A (en) 1989-08-25 1989-08-25 Production of biologically active insulin-like growth factor i from high expression host cell systems

Publications (1)

Publication Number Publication Date
ES2089029T3 true ES2089029T3 (es) 1996-10-01

Family

ID=23576440

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90914315T Expired - Lifetime ES2089029T3 (es) 1989-08-25 1990-08-21 Produccion de factor de crecimiento similar a la insulina i biologicamente activo a partir de sistemas de celulas hospedantes de expresion elevada.

Country Status (10)

Country Link
US (1) US5158875A (es)
EP (1) EP0441955B1 (es)
JP (1) JPH04501514A (es)
AT (1) ATE140029T1 (es)
AU (1) AU644105B2 (es)
CA (1) CA2023969A1 (es)
DE (1) DE69027666T2 (es)
DK (1) DK0441955T3 (es)
ES (1) ES2089029T3 (es)
WO (1) WO1991002807A1 (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112892220A (zh) * 2021-01-19 2021-06-04 南开大学 一种高效的活性蛋白分离方法与装置

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5316932A (en) * 1989-03-24 1994-05-31 Duke University Homogeneous denatured human 06-guanine alkyltransferase prepared by immunoaffinity chromatography using monoclonal antibody specific for enzyme
AU8417091A (en) * 1990-08-20 1992-03-17 Novo Nordisk A/S Biologically active compound, a process for the preparation thereof and use of the same
US5646016A (en) * 1991-02-06 1997-07-08 Genetics Institute, Inc. Peptide and protein fusions to thioredoxin, thioredoxin-like molecules, and modified thioredoxin-like molecules
US5378613A (en) * 1991-09-24 1995-01-03 Eli Lilly And Company Method for increased expression of low molecular weight recombinant polypeptides
US5288931A (en) * 1991-12-06 1994-02-22 Genentech, Inc. Method for refolding insoluble, misfolded insulin-like growth factor-I into an active conformation
US5958764A (en) * 1992-04-30 1999-09-28 Baylor College Of Medicine Specific expression vectors and methods of use
EP0695359B1 (en) * 1993-03-29 1997-06-04 E.I. Du Pont De Nemours & Company Incorporated A method for enhancing the production of biologically active recombinant proteins
US5407810A (en) * 1993-08-20 1995-04-18 Genentech, Inc. Aqueous multiple-phase isolation of polypeptide
US5663304A (en) * 1993-08-20 1997-09-02 Genentech, Inc. Refolding of misfolded insulin-like growth factor-I
SE9303784D0 (sv) * 1993-11-16 1993-11-16 Kabi Pharmacia Ab Igf
US5451660A (en) * 1993-12-13 1995-09-19 Genentech, Inc. Method for purifying polypeptides
WO1995016708A1 (en) * 1993-12-17 1995-06-22 Novo Nordisk A/S Proinsulin-like compounds
JPH10504971A (ja) * 1994-09-08 1998-05-19 カイロン コーポレイション インスリン様増殖因子の改善された生産方法
US5712249A (en) * 1994-09-08 1998-01-27 Ciba-Geigy Corporation Use of insulin-like growth factors I and II for inhibition of inflammatory response
US5789547A (en) * 1995-06-07 1998-08-04 Celtrix Pharmaceuticals, Inc. Method of producing insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-3 (IGFBP-3) with correct folding and disulfide bonding
CA2396320A1 (en) * 2000-01-11 2001-07-19 Maxygen, Inc. Integrated systems and methods for diversity generation and screening
US20080119433A1 (en) * 2006-07-06 2008-05-22 Aaron Thomas Tabor Compositions and Methods for Genetic Modification of Cells Having Cosmetic Function to Enhance Cosmetic Appearance
CL2007002502A1 (es) * 2006-08-31 2008-05-30 Hoffmann La Roche Variantes del factor de crecimiento similar a insulina-1 humano (igf-1) pegilados en lisina; metodo de produccion; proteina de fusion que la comprende; y su uso para tratar la enfermedad de alzheimer.
MX2009001691A (es) * 2006-08-31 2009-02-25 Hoffmann La Roche Metodo para produccion del factor de crecimiento i tipo insulina.
BRPI0910338A2 (pt) * 2008-04-03 2020-08-18 F. Hoffmann-La Roche Ag uso de variantes peguiladas de igf-i para o tratamento de distúbios neuromusculares
WO2009121551A1 (en) * 2008-04-03 2009-10-08 F. Hoffmann-La Roche Ag Pegylated insulin-like-growth-factor assay
EP2454368A4 (en) 2009-07-17 2013-01-09 Aaron Thomas Tabor COMPOSITIONS AND METHOD FOR THE GENETIC MODIFICATION OF COSMETIC FUNCTION CELLS FOR IMPROVING THE COSMETIC APPEARANCE PICTURE
US20110152188A1 (en) * 2009-12-23 2011-06-23 Hanns-Christian Mahler Pharmaceutical compositions of igf/i proteins
US9675671B2 (en) * 2014-01-12 2017-06-13 Igf Oncology, Llc Fusion proteins containing insulin-like growth factor-1 and epidermal growth factor and variants thereof and uses thereof
CN114292800B (zh) * 2021-12-27 2024-08-20 武汉佰乐博生物技术有限公司 用于igf-1基因重组表达的重组细胞和重组表达方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4532207A (en) * 1982-03-19 1985-07-30 G. D. Searle & Co. Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase
FR2594846B1 (fr) * 1986-02-21 1989-10-20 Genetica Procede de preparation de la serum albumine humaine mature
AU1987088A (en) * 1987-06-24 1989-01-19 Novo Nordisk A/S A process for preparing a protein or polypeptide, a dna sequence coding for the polypeptide, a microorganism containing the dna sequence as well as the polypeptide and its use as a pharmaceutical preparation

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112892220A (zh) * 2021-01-19 2021-06-04 南开大学 一种高效的活性蛋白分离方法与装置
CN112892220B (zh) * 2021-01-19 2023-02-17 南开大学 一种高效的活性蛋白分离方法与装置

Also Published As

Publication number Publication date
CA2023969A1 (en) 1991-02-26
EP0441955A4 (en) 1992-07-15
DK0441955T3 (da) 1996-10-07
JPH04501514A (ja) 1992-03-19
EP0441955B1 (en) 1996-07-03
DE69027666T2 (de) 1996-11-07
WO1991002807A1 (en) 1991-03-07
AU6425490A (en) 1991-04-03
EP0441955A1 (en) 1991-08-21
ATE140029T1 (de) 1996-07-15
AU644105B2 (en) 1993-12-02
US5158875A (en) 1992-10-27
DE69027666D1 (de) 1996-08-08

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