AR126768A1 - Elementos reguladores de plantas y usos de los mismos - Google Patents

Elementos reguladores de plantas y usos de los mismos

Info

Publication number
AR126768A1
AR126768A1 ARP220102166A ARP220102166A AR126768A1 AR 126768 A1 AR126768 A1 AR 126768A1 AR P220102166 A ARP220102166 A AR P220102166A AR P220102166 A ARP220102166 A AR P220102166A AR 126768 A1 AR126768 A1 AR 126768A1
Authority
AR
Argentina
Prior art keywords
seq
sequence
plant
dna molecule
fragment
Prior art date
Application number
ARP220102166A
Other languages
English (en)
Inventor
Jaishree M Chittoor
Stanislaw Flasinski
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of AR126768A1 publication Critical patent/AR126768A1/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223Vegetative tissue-specific promoters
    • C12N15/8227Root-specific
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223Vegetative tissue-specific promoters
    • C12N15/8229Meristem-specific, e.g. nodal, apical
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

La invención proporciona constructos y moléculas de ADN recombinante, así como sus secuencias de nucleótidos, útiles para modular la expresión génica en plantas. La invención también proporciona plantas, células de plantas, partes de plantas y semillas transgénicas que comprenden moléculas de ADN recombinante unidas operativamente a moléculas de ADN transcribibles heterólogas. También se proporcionan métodos para el uso de constructos y moléculas de ADN recombinante y las plantas, células de plantas, partes de plantas y semillas transgénicas que comprenden los constructos y moléculas de ADN recombinante. Reivindicación 1: Una molécula de ADN recombinante que comprende una secuencia de ADN seleccionada del grupo que consiste en: a) una secuencia con al menos un 85 por ciento de identidad de secuencia con cualquiera de las SEQ ID Nº 1 - 3; b) una secuencia que comprende cualquiera de las SEQ ID Nº 1 - 3; y c) un fragmento de cualquiera de las SEQ ID Nº 1 - 3, en donde el fragmento tiene actividad reguladora de genes; en donde dicha secuencia está unida operativamente a una molécula de ADN transcribible heteróloga. Reivindicación 9: Una célula vegetal transgénica que comprende una molécula de ADN recombinante que comprende una secuencia seleccionada del grupo que consiste en: a) una secuencia con al menos un 85 por ciento de identidad de secuencia con cualquiera de las SEQ ID Nº 1 - 3; b) una secuencia que comprende cualquiera de las SEQ ID Nº 1 - 3; y c) un fragmento de cualquiera de las SEQ ID Nº 1 - 3, en donde el fragmento tiene actividad reguladora de genes; en donde dicha secuencia está unida operativamente a una molécula de ADN transcribible heteróloga. Reivindicación 15: Un método para producir un producto básico que comprende obtener una planta transgénica o parte de la misma de acuerdo con la reivindicación 12 y producir el producto básico a partir de la misma. Reivindicación 17: Un método para expresar una molécula de ADN transcribible que comprende obtener una planta transgénica de acuerdo con la reivindicación 12 y cultivar la planta, en donde se expresa el ADN transcribible. Reivindicación 18: Un método de expresión preferencial de una molécula de ADN transcribible en tejido de raíz, que comprende: a) transformar una célula de planta con un vector que comprende un promotor con al menos un 85 por ciento de identidad de secuencia con la SEQ ID Nº 2 o un fragmento activo del mismo unido operativamente a una molécula de ADN transcribible heteróloga; e b) inducir a la célula a formar una planta.
ARP220102166A 2021-08-17 2022-08-11 Elementos reguladores de plantas y usos de los mismos AR126768A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US202163234175P 2021-08-17 2021-08-17

Publications (1)

Publication Number Publication Date
AR126768A1 true AR126768A1 (es) 2023-11-08

Family

ID=85239812

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP220102166A AR126768A1 (es) 2021-08-17 2022-08-11 Elementos reguladores de plantas y usos de los mismos

Country Status (12)

Country Link
US (1) US20230077295A1 (es)
EP (1) EP4388112A2 (es)
JP (1) JP2024530961A (es)
KR (1) KR20240046179A (es)
CN (1) CN117897491A (es)
AR (1) AR126768A1 (es)
AU (1) AU2022331418A1 (es)
CA (1) CA3229453A1 (es)
CL (1) CL2024000453A1 (es)
CO (1) CO2024001499A2 (es)
MX (1) MX2024002065A (es)
WO (1) WO2023023485A2 (es)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT1240340E (pt) * 1999-12-16 2012-08-01 Monsanto Technology Llc Construções de dna para expressão de polipéptidos heterólogos em plantas
US7491813B2 (en) * 2005-12-07 2009-02-17 Monsanto Technology Llc Promoter polynucleotides identified from Zea mays for use in plants
CL2009000644A1 (es) * 2008-03-17 2009-10-09 Monsanto Tech Llc Sociedad Organizada Bajo Las Leyes Del Estado De Delaware Promotores quimericos que comprenden una primera y segunda moleculas de adn capaz de proporcionar expresion aumentada en semillas, construccion de adn y celula de planta transgenica que los comprenden.
ES2457218T3 (es) * 2008-04-07 2014-04-25 Monsanto Technology Llc Elementos reguladores de plantas y usos de los mismos
EP2524045B1 (en) * 2010-01-14 2015-12-02 Monsanto Technology LLC Plant regulatory elements and uses thereof
AU2012310193A1 (en) * 2011-09-15 2014-03-06 Basf Plant Science Company Gmbh Regulatory nucleic acid molecules for reliable gene expression in plants

Also Published As

Publication number Publication date
CL2024000453A1 (es) 2024-06-21
WO2023023485A3 (en) 2023-03-30
WO2023023485A2 (en) 2023-02-23
CN117897491A (zh) 2024-04-16
EP4388112A2 (en) 2024-06-26
CA3229453A1 (en) 2023-02-23
KR20240046179A (ko) 2024-04-08
CO2024001499A2 (es) 2024-02-26
MX2024002065A (es) 2024-03-01
AU2022331418A1 (en) 2024-02-22
US20230077295A1 (en) 2023-03-09
JP2024530961A (ja) 2024-08-27

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