AR125731A1 - METHOD TO CREATE NEW GENE IN AN ORGANISM AND ITS USE - Google Patents

METHOD TO CREATE NEW GENE IN AN ORGANISM AND ITS USE

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Publication number
AR125731A1
AR125731A1 ARP220101136A ARP220101136A AR125731A1 AR 125731 A1 AR125731 A1 AR 125731A1 AR P220101136 A ARP220101136 A AR P220101136A AR P220101136 A ARP220101136 A AR P220101136A AR 125731 A1 AR125731 A1 AR 125731A1
Authority
AR
Argentina
Prior art keywords
different
organism
gene
new gene
new
Prior art date
Application number
ARP220101136A
Other languages
Spanish (es)
Inventor
Linjian Jiang
Jiyao Wang
Sudong Mo
Bo Chen
Qiang Hu
Dehui Ding
Huarong Li
Original Assignee
Qingdao Kingagroot Chemical Compound Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qingdao Kingagroot Chemical Compound Co Ltd filed Critical Qingdao Kingagroot Chemical Compound Co Ltd
Publication of AR125731A1 publication Critical patent/AR125731A1/en

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Abstract

La presente invención se refiere a los campos técnicos de ingeniería genética y bioinformática, en particular, se refiere a un método para crear un nuevo gen en un organismo en ausencia de una plantilla de ADN artificial, y a su uso. El método comprende generar simultáneamente roturas de ADN en dos o más diferentes sitios específicos en el genoma del organismo, en donde los sitios específicos son sitios genómicos capaces de separar diferentes elementos génicos o diferentes dominios de proteínas, y las roturas de ADN se ligan entre sí a través de empalme de extremos no homólogos (NHEJ) o reparación homóloga para generar una nueva combinación de los diferentes elementos génicos o diferentes dominios de proteínas que son diferentes de la secuencia genómica original, por medio de lo cual, se crea un nuevo gen. El nuevo gen de la invención puede cambiar el crecimiento, desarrollo, resistencia, rendimiento y otros atributos del organismo, y tiene gran valor en términos de aplicación.The present invention relates to the technical fields of genetic engineering and bioinformatics, in particular, it relates to a method for creating a new gene in an organism in the absence of an artificial DNA template, and its use. The method comprises simultaneously generating DNA breaks at two or more different specific sites in the organism's genome, where the specific sites are genomic sites capable of separating different gene elements or different protein domains, and the DNA breaks are ligated together. through non-homologous end splicing (NHEJ) or homologous repair to generate a new combination of different gene elements or different protein domains that are different from the original genomic sequence, whereby a new gene is created. The new gene of the invention can change the growth, development, resistance, performance and other attributes of the organism, and has great value in terms of application.

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