AR113150A1 - Producción mejorada de etanol libre de glicerol - Google Patents

Producción mejorada de etanol libre de glicerol

Info

Publication number
AR113150A1
AR113150A1 ARP180102800A ARP180102800A AR113150A1 AR 113150 A1 AR113150 A1 AR 113150A1 AR P180102800 A ARP180102800 A AR P180102800A AR P180102800 A ARP180102800 A AR P180102800A AR 113150 A1 AR113150 A1 AR 113150A1
Authority
AR
Argentina
Prior art keywords
nucleotide sequence
glycerol
sequence encoding
improved production
free ethanol
Prior art date
Application number
ARP180102800A
Other languages
English (en)
Inventor
Lutz Ingrid Maria Vugt-Van
Waal Paulus Petrus De
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Publication of AR113150A1 publication Critical patent/AR113150A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01008—Glycerol-3-phosphate dehydrogenase (NAD+) (1.1.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/05—Oxidoreductases acting on the CH-OH group of donors (1.1) with a quinone or similar compound as acceptor (1.1.5)
    • C12Y101/05003—Glycerol-3-phosphate dehydrogenase (1.1.5.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01019—Phosphoribulokinase (2.7.1.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0102—Alpha-glucosidase (3.2.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01039—Ribulose-bisphosphate carboxylase (4.1.1.39)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/0103—Glycerol dehydratase (4.2.1.30)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Levadura recombinante que comprende una secuencia de nucleótidos que codifica una glicerol deshidrogenasa, una secuencia de nucleótidos que codifica una ribulosa-1,5-bifosfato carboxilasa oxigenasa (EC 4.1.1.39); una secuencia de nucleótidos que codifica una fosforribuloquinasa (EC 2.7.1.19); una secuencia de nucleótidos que permite la expresión de una glucoamilasa (EC 3.2.1.20 o 3.2.1.3); y opcionalmente una secuencia de nucleótidos que codifica un transportador de glicerol. Esta célula puede usarse para la producción de etanol y ventajosamente produce poco o nada de glicerol.
ARP180102800A 2017-09-29 2018-09-28 Producción mejorada de etanol libre de glicerol AR113150A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP17193914 2017-09-29

Publications (1)

Publication Number Publication Date
AR113150A1 true AR113150A1 (es) 2020-01-29

Family

ID=60001718

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP180102800A AR113150A1 (es) 2017-09-29 2018-09-28 Producción mejorada de etanol libre de glicerol

Country Status (3)

Country Link
US (1) US11384368B2 (es)
AR (1) AR113150A1 (es)
WO (1) WO2019063542A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20160002692A (ko) * 2013-02-22 2016-01-08 테크니쉐 유니버시테이트 델프트 증가된 생산 수율을 갖는 방법에 사용하기 위한 재조합 미생물
BR112022007870A2 (pt) * 2019-11-08 2022-07-12 Dsm Ip Assets Bv Processo para produzir etanol
WO2023079050A1 (en) * 2021-11-04 2023-05-11 Dsm Ip Assets B.V. Recombinant yeast cell
WO2023079049A1 (en) 2021-11-04 2023-05-11 Dsm Ip Assets B.V. Variant polypeptide and recombinant yeast cell
WO2023079048A1 (en) 2021-11-04 2023-05-11 Dsm Ip Assets B.V. Process for the production of ethanol and recombinant yeast cell

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2007302867B2 (en) 2006-10-02 2013-11-07 Dsm Ip Assets B.V. Metabolic engineering of arabinose-fermenting yeast cells
BRPI0813710B1 (pt) 2007-07-23 2018-10-09 Dsm Ip Assets Bv método de identificar um polipeptídeo heterólogo tendo atividade enzimática para converter piruvato, acetaldeído ou acetato em acetil-coa no citosol de uma célula de levedura, vetor para a expressão de polipeptídeos heterólogos em levedura, célula de levedura recombinante e método de produzir um produto de fermentação
WO2009112472A2 (en) 2008-03-13 2009-09-17 Dsm Ip Assets B.V. Selection of organisms capable of fermenting mixed substrates
AU2012346662B2 (en) 2011-11-30 2016-07-28 Danisco Us Inc. Yeast strains engineered to produce ethanol from acetic acid and glycerol
KR20160002692A (ko) * 2013-02-22 2016-01-08 테크니쉐 유니버시테이트 델프트 증가된 생산 수율을 갖는 방법에 사용하기 위한 재조합 미생물
MX2016001881A (es) * 2013-08-15 2016-08-03 Lallemand Hungary Liquidity Man Llc Metodos para la mejora de rendimiento y produccion de producto en un microorganismo a traves del reciclaje de glicerol.
AR097480A1 (es) 2013-08-29 2016-03-16 Dsm Ip Assets Bv Células de levadura convertidoras de glicerol y ácido acético con una conversión de ácido acético mejorada
MX2018005692A (es) * 2015-11-06 2019-03-14 Lallemand Hungary Liquidity Man Llc Limitación de trehalosa producida por levaduras en fermentación.
EP3559220A1 (en) 2016-12-23 2019-10-30 DSM IP Assets B.V. Improved glycerol free ethanol production

Also Published As

Publication number Publication date
US11384368B2 (en) 2022-07-12
US20200239914A1 (en) 2020-07-30
WO2019063542A1 (en) 2019-04-04

Similar Documents

Publication Publication Date Title
AR113150A1 (es) Producción mejorada de etanol libre de glicerol
BR112019010526A2 (pt) produção aprimorada de etanol livre de glicerol
MX2022001018A (es) Electrodos metalicos de bajo costo.
TWD194917S (zh) 太陽能電池
CR20180525A (es) Producción de ácido ribonucleico libre de células
WO2018213661A3 (en) PASSIVATION AGENTS FOR ELECTROCHEMICAL CELLS
BR112017000721A2 (pt) eléctrodo para a produção de divisão de água, eletrolisador de água, célula cloro alcalina e respectivo método de fabrico
AR094859A1 (es) Microorganismo recombinante para utilizar en método con producción aumentada de producto
EP3493311A4 (en) MEMBRANE ELECTRODES ARRANGEMENT, METHOD FOR PRODUCING THE SAME AND FUEL CELL THEREFOR
EA201992795A1 (ru) Способ получения эукариотических клеток с отредактированной днк и набор, используемый в этом способе
BR112021020450A2 (pt) Processos para fabricação e uso de um secretoma derivado de células-tronco mesenquimais
TWD198906S (zh) 無柵線太陽能電池
RS54073B1 (en) STROMAL MATERIAL CELLS
EP3223350A4 (en) Anode for solid oxide fuel cell and manufacturing method therefor, and manufacturing method for electrolyte layer-electrode assembly for fuel cell
BR112016016524A2 (pt) célula de levedura transformada
EP3446781A4 (en) ELECTROCATALYST, MEMBRANE ELECTRODE ASSEMBLY USING THE ELECTROCATALYST, AND FUEL CELL
TWD200419S (zh) 太陽能電池
CR20170485A (es) Producción de ácido ribonucleico libre de células
EP3536664A4 (en) METHOD FOR PRODUCING AN ELECTRODE, ELECTRODE PRODUCED BY THIS, MEMBRANE ELECTRODE ARRANGEMENT WITH THE ELECTRODE, AND FUEL CELL WITH THE MEMBRANE ELECTRODE ARRANGEMENT
EP3520892A4 (en) SUPPORT, ELECTRODE FOR FUEL CELL, MEMBRANE-ELECTRODE ASSEMBLY, AND FUEL CELL COMPRISING SAME
EP3113264A4 (en) Gas diffusion electrode substrate, and membrane electrode assembly and fuel cell equipped with same
EP3113263A4 (en) Gas diffusion electrode substrate, and membrane electrode assembly and fuel cell provided therewith
WO2018093240A3 (ko) 전기화학소자용 전극 및 이를 제조하는 방법
EP3389123A4 (en) CARBON LAYER, GAS DIFFUSION ELECTRODE SUBSTRATE AND FUEL CELL
BR112018007971A2 (pt) bateria de hidreto metálico com adição de gás de hidrogênio, gás de oxigênio ou peróxido de hidrogênio