AR103092A2 - Método para preparar una bacteria del género sphingomonas que comprende una mutación, una cepa mutante de sphingomonas, bacteria aislada y purificada, método para producir un polisacárido sphingan que comprende cultivar la bacteria, y caldo de cultivo que la comprende - Google Patents

Método para preparar una bacteria del género sphingomonas que comprende una mutación, una cepa mutante de sphingomonas, bacteria aislada y purificada, método para producir un polisacárido sphingan que comprende cultivar la bacteria, y caldo de cultivo que la comprende

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Publication number
AR103092A2
AR103092A2 ARP150104167A ARP150104167A AR103092A2 AR 103092 A2 AR103092 A2 AR 103092A2 AR P150104167 A ARP150104167 A AR P150104167A AR P150104167 A ARP150104167 A AR P150104167A AR 103092 A2 AR103092 A2 AR 103092A2
Authority
AR
Argentina
Prior art keywords
sphingomonas
cultivating
bacteria
sphingan
mutation
Prior art date
Application number
ARP150104167A
Other languages
English (en)
Inventor
Patel Yamini
E Harding Nancy
Original Assignee
Cp Kelco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cp Kelco Us Inc filed Critical Cp Kelco Us Inc
Publication of AR103092A2 publication Critical patent/AR103092A2/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

Un método para preparar una bacteria del género Sphingomonas que comprende una mutación en los genes gelR o dpsR del conjunto de genes biosintéticos de polisacáridos sphingan, que comprende: aislar un segmento de ADN genómico de una primera bacteria del género Sphingomonas, donde el segmento comprende la totalidad o una parte del gen gelR o del gen dpsR del conjunto de genes biosintéticos de polisacáridos sphingan; inducir una mutación en el segmento para formar un segmento mutado; introducir el segmento mutado en una segunda bacteria del género Sphingomonas, donde la segunda bacteria comprende genes gelR o dpsR de tipo salvaje del conjunto de genes biosintéticos de polisacáridos sphingan; aislar una progenie de la segunda bacteria en cuyo genoma se ha integrado el segmento mutado y se ha reemplazado el gelR o el gen dpsR de tipo salvaje del conjunto de genes biosintéticos de polisacáridos sphingan de la segunda bacteria.
ARP150104167A 2005-02-04 2015-12-17 Método para preparar una bacteria del género sphingomonas que comprende una mutación, una cepa mutante de sphingomonas, bacteria aislada y purificada, método para producir un polisacárido sphingan que comprende cultivar la bacteria, y caldo de cultivo que la comprende AR103092A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US64955905P 2005-02-04 2005-02-04

Publications (1)

Publication Number Publication Date
AR103092A2 true AR103092A2 (es) 2017-04-12

Family

ID=36953794

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP060100398A AR052111A1 (es) 2005-02-04 2006-02-03 Supresiones geneticas dirigidas para polisacaridos formadores de limo
ARP150104167A AR103092A2 (es) 2005-02-04 2015-12-17 Método para preparar una bacteria del género sphingomonas que comprende una mutación, una cepa mutante de sphingomonas, bacteria aislada y purificada, método para producir un polisacárido sphingan que comprende cultivar la bacteria, y caldo de cultivo que la comprende

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ARP060100398A AR052111A1 (es) 2005-02-04 2006-02-03 Supresiones geneticas dirigidas para polisacaridos formadores de limo

Country Status (5)

Country Link
US (7) US7888333B2 (es)
CN (5) CN103087940B (es)
AR (2) AR052111A1 (es)
TW (1) TWI467015B (es)
WO (1) WO2006096269A2 (es)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110027838A1 (en) 2009-07-31 2011-02-03 Harding Nancy E Sphingomonas Strains Producing Greatly Increased Yield Of PHB-Deficient Sphingan (Diutan)
US8343896B2 (en) 2005-01-24 2013-01-01 Halliburton Energy Services, Inc. Sealant compositions comprising diutan and associated methods
CN103087940B (zh) * 2005-02-04 2015-05-13 Cp凯尔科美国公司 多糖粘液形成菌的靶基因缺失
AP2013006963A0 (en) 2005-09-07 2013-07-31 Amgen Fremont Inc Human monoclonal antibodies to activin receptor-like Kinase-1
AU2015200175A1 (en) * 2005-11-01 2015-02-05 Cp Kelco U.S., Inc. High viscosity diutan gums and methods of producing
US7868167B2 (en) 2005-11-01 2011-01-11 Cp Kelco U.S., Inc. High viscosity diutan gums
AP2878A (en) * 2005-11-01 2014-03-31 Cp Kelco Us Inc High viscocity diutan gums and methods of producing
ES2397094T3 (es) * 2005-11-01 2015-05-06 Cp Kelco Us, Inc. Gomas diutan de alta viscosidad y métodos para producirlas
US7910524B2 (en) * 2007-02-08 2011-03-22 Halliburton Energy Services, Inc. Treatment fluids comprising diutan and associated methods
US7584791B2 (en) 2007-02-08 2009-09-08 Halliburton Energy Services, Inc. Methods for reducing the viscosity of treatment fluids comprising diutan
US9016375B2 (en) 2011-11-30 2015-04-28 Halliburton Energy Services, Inc. Breaking diutan with oxalic acid at 180° F to 220° F
US20130333886A1 (en) 2012-06-19 2013-12-19 Halliburton Energy Services, Inc. Breaking diutan with metal activitor down to 140 °f or lower
CN103305566B (zh) * 2013-06-14 2014-12-10 浙江大学 一种提高细菌多糖产量及粘度的培养方法
CN106977618B (zh) * 2017-04-13 2022-07-01 张星昊 一种从定优胶发酵液中提取定优胶的方法
CN109735556A (zh) * 2019-02-22 2019-05-10 昆明理工大学 引导糖基转移酶基因的用途
CN110144318B (zh) * 2019-04-22 2021-03-26 南京工业大学 无色素低分子量威兰胶生产菌株及其构建方法与应用
FR3113494A1 (fr) * 2020-08-24 2022-02-25 IFP Energies Nouvelles Procédé de production de Welan de haute viscosité parSphingomonas sp .
US11694876B2 (en) 2021-12-08 2023-07-04 Applied Materials, Inc. Apparatus and method for delivering a plurality of waveform signals during plasma processing

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5985623A (en) 1995-01-24 1999-11-16 Shin-Etsu Bio, Inc. DNA segments and methods for increasing polysaccharide production
US6290814B1 (en) * 1999-03-31 2001-09-18 Penford Corporation Paper coating composition comprising gellan gum/starch blend
AU2001247281A1 (en) * 2000-03-02 2001-09-12 Cp Kelco U.S., Inc. Mutant bacterial strains of the genus sphingonomas deficient in production of polyhydroxybutyrate and a process of clarification of sphingans and compositions thereof
CA2351233C (en) * 2000-07-24 2010-10-19 Shin-Etsu Chemical Co., Ltd. Production of exopolysaccharides unattached to the surface of bacterial cells
US20030100078A1 (en) 2001-07-03 2003-05-29 Harding Nancy E. Mutant strain of Sphingomonas elodea which produces non-acetylated gellan gum
US6620775B2 (en) * 2001-11-26 2003-09-16 Cp Kelco U.S. Inc. Viscosity stabilization in alkaline solutions
CN103087940B (zh) * 2005-02-04 2015-05-13 Cp凯尔科美国公司 多糖粘液形成菌的靶基因缺失

Also Published As

Publication number Publication date
US20170002367A1 (en) 2017-01-05
US10351858B2 (en) 2019-07-16
CN103087940B (zh) 2015-05-13
AR052111A1 (es) 2007-02-28
US9422567B2 (en) 2016-08-23
WO2006096269A2 (en) 2006-09-14
US20060199201A1 (en) 2006-09-07
CN101501213B (zh) 2013-07-17
US9845476B2 (en) 2017-12-19
US8716003B2 (en) 2014-05-06
US20110281334A1 (en) 2011-11-17
TWI467015B (zh) 2015-01-01
CN103087940A (zh) 2013-05-08
US20130203153A1 (en) 2013-08-08
TW200641124A (en) 2006-12-01
CN103087941B (zh) 2015-09-16
WO2006096269A3 (en) 2008-12-04
CN103087970B (zh) 2016-12-21
US20110171721A1 (en) 2011-07-14
US20180127762A1 (en) 2018-05-10
US8759071B2 (en) 2014-06-24
US8709782B2 (en) 2014-04-29
CN103087941A (zh) 2013-05-08
CN103146034A (zh) 2013-06-12
CN103087970A (zh) 2013-05-08
CN101501213A (zh) 2009-08-05
US7888333B2 (en) 2011-02-15
US20140342436A1 (en) 2014-11-20
CN103146034B (zh) 2016-01-20

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