AR100235A1 - COMPOSITIONS OF OLIGONUCLEOTIDES AND METHODS TO PERFORM THEM - Google Patents
COMPOSITIONS OF OLIGONUCLEOTIDES AND METHODS TO PERFORM THEMInfo
- Publication number
- AR100235A1 AR100235A1 ARP150101301A ARP150101301A AR100235A1 AR 100235 A1 AR100235 A1 AR 100235A1 AR P150101301 A ARP150101301 A AR P150101301A AR P150101301 A ARP150101301 A AR P150101301A AR 100235 A1 AR100235 A1 AR 100235A1
- Authority
- AR
- Argentina
- Prior art keywords
- compositions
- oligonucleotides
- bond
- amino group
- phosphoramidite
- Prior art date
Links
Abstract
La presente descripción proporciona un método de fase sólida para realizar oligonucleótidos mediante ciclos de acoplamiento secuenciales que incluyen al menos un acoplamiento de una subunidad de dímero de dinucleótido con un grupo de extremo 3 libre de una cadena en crecimiento. Los oligonucleótidos incluyen al menos dos subunidades de nucleósido unidas por un enlace fosforamidato N3®P5. El método puede incluir los pasos de (a) desproteger el grupo amino 3 protegido de un nucleósido de extremo unido a un soporte de fase sólida, dicha desprotección forma un grupo amino 3 libre; (b) poner en contacto el grupo amino 3 libre con un dímero de amino-dinucleótido-5-fosforamidita 3 protegido en presencia de un catalizador nucleofílico para formar un enlace de fosforamidita N3®P5 internucleósido; y (c) oxidar (por ejemplo, sulfar) el enlace. Las composiciones producidas por los métodos de la presente pueden incluir una cantidad reducida de uno o más productos de oligonucléotido (N-x). También se proporcionan composiciones farmacéuticas que incluyen las composiciones de oligonucleótido de la presente.The present description provides a solid phase method for performing oligonucleotides by sequential coupling cycles that include at least one coupling of a dinucleotide dimer subunit with a free end group 3 of a growing chain. The oligonucleotides include at least two nucleoside subunits linked by an N3®P5 phosphoramidate bond. The method may include the steps of (a) deprotecting the protected amino group 3 from an end nucleoside attached to a solid phase support, said deprotection forms a free amino group 3; (b) contacting the free amino group 3 with a protected amino-dinucleotide-5-phosphoramidite 3 dimer in the presence of a nucleophilic catalyst to form an internucleoside N3®P5 phosphoramidite bond; and (c) oxidize (for example, sulfar) the bond. Compositions produced by the methods herein may include a reduced amount of one or more oligonucleotide products (N-x). Pharmaceutical compositions that include the oligonucleotide compositions herein are also provided.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201461987396P | 2014-05-01 | 2014-05-01 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR100235A1 true AR100235A1 (en) | 2016-09-21 |
Family
ID=58700032
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP150101301A AR100235A1 (en) | 2014-05-01 | 2015-04-29 | COMPOSITIONS OF OLIGONUCLEOTIDES AND METHODS TO PERFORM THEM |
ARP200100967A AR118606A2 (en) | 2014-05-01 | 2020-04-06 | UNPURIFIED SYNTHETIC COMPOSITION AND RELATED COMPOUND |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP200100967A AR118606A2 (en) | 2014-05-01 | 2020-04-06 | UNPURIFIED SYNTHETIC COMPOSITION AND RELATED COMPOUND |
Country Status (2)
Country | Link |
---|---|
AR (2) | AR100235A1 (en) |
MA (3) | MA39398A1 (en) |
-
2015
- 2015-04-29 MA MA39398A patent/MA39398A1/en unknown
- 2015-04-29 MA MA48154A patent/MA48154B1/en unknown
- 2015-04-29 AR ARP150101301A patent/AR100235A1/en unknown
-
2016
- 2016-10-20 MA MA59673A patent/MA59673A1/en unknown
-
2020
- 2020-04-06 AR ARP200100967A patent/AR118606A2/en unknown
Also Published As
Publication number | Publication date |
---|---|
MA48154A1 (en) | 2021-09-30 |
MA48154B1 (en) | 2022-05-31 |
AR118606A2 (en) | 2021-10-20 |
MA39398A1 (en) | 2019-03-29 |
MA59673A1 (en) | 2023-04-28 |
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