AR100235A1 - COMPOSITIONS OF OLIGONUCLEOTIDES AND METHODS TO PERFORM THEM - Google Patents

COMPOSITIONS OF OLIGONUCLEOTIDES AND METHODS TO PERFORM THEM

Info

Publication number
AR100235A1
AR100235A1 ARP150101301A ARP150101301A AR100235A1 AR 100235 A1 AR100235 A1 AR 100235A1 AR P150101301 A ARP150101301 A AR P150101301A AR P150101301 A ARP150101301 A AR P150101301A AR 100235 A1 AR100235 A1 AR 100235A1
Authority
AR
Argentina
Prior art keywords
compositions
oligonucleotides
bond
amino group
phosphoramidite
Prior art date
Application number
ARP150101301A
Other languages
Spanish (es)
Inventor
H Ramiya Premchandran
Original Assignee
Geron Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Geron Corp filed Critical Geron Corp
Publication of AR100235A1 publication Critical patent/AR100235A1/en

Links

Abstract

La presente descripción proporciona un método de fase sólida para realizar oligonucleótidos mediante ciclos de acoplamiento secuenciales que incluyen al menos un acoplamiento de una subunidad de dímero de dinucleótido con un grupo de extremo 3 libre de una cadena en crecimiento. Los oligonucleótidos incluyen al menos dos subunidades de nucleósido unidas por un enlace fosforamidato N3®P5. El método puede incluir los pasos de (a) desproteger el grupo amino 3 protegido de un nucleósido de extremo unido a un soporte de fase sólida, dicha desprotección forma un grupo amino 3 libre; (b) poner en contacto el grupo amino 3 libre con un dímero de amino-dinucleótido-5-fosforamidita 3 protegido en presencia de un catalizador nucleofílico para formar un enlace de fosforamidita N3®P5 internucleósido; y (c) oxidar (por ejemplo, sulfar) el enlace. Las composiciones producidas por los métodos de la presente pueden incluir una cantidad reducida de uno o más productos de oligonucléotido (N-x). También se proporcionan composiciones farmacéuticas que incluyen las composiciones de oligonucleótido de la presente.The present description provides a solid phase method for performing oligonucleotides by sequential coupling cycles that include at least one coupling of a dinucleotide dimer subunit with a free end group 3 of a growing chain. The oligonucleotides include at least two nucleoside subunits linked by an N3®P5 phosphoramidate bond. The method may include the steps of (a) deprotecting the protected amino group 3 from an end nucleoside attached to a solid phase support, said deprotection forms a free amino group 3; (b) contacting the free amino group 3 with a protected amino-dinucleotide-5-phosphoramidite 3 dimer in the presence of a nucleophilic catalyst to form an internucleoside N3®P5 phosphoramidite bond; and (c) oxidize (for example, sulfar) the bond. Compositions produced by the methods herein may include a reduced amount of one or more oligonucleotide products (N-x). Pharmaceutical compositions that include the oligonucleotide compositions herein are also provided.

ARP150101301A 2014-05-01 2015-04-29 COMPOSITIONS OF OLIGONUCLEOTIDES AND METHODS TO PERFORM THEM AR100235A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201461987396P 2014-05-01 2014-05-01

Publications (1)

Publication Number Publication Date
AR100235A1 true AR100235A1 (en) 2016-09-21

Family

ID=58700032

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP150101301A AR100235A1 (en) 2014-05-01 2015-04-29 COMPOSITIONS OF OLIGONUCLEOTIDES AND METHODS TO PERFORM THEM
ARP200100967A AR118606A2 (en) 2014-05-01 2020-04-06 UNPURIFIED SYNTHETIC COMPOSITION AND RELATED COMPOUND

Family Applications After (1)

Application Number Title Priority Date Filing Date
ARP200100967A AR118606A2 (en) 2014-05-01 2020-04-06 UNPURIFIED SYNTHETIC COMPOSITION AND RELATED COMPOUND

Country Status (2)

Country Link
AR (2) AR100235A1 (en)
MA (3) MA39398A1 (en)

Also Published As

Publication number Publication date
MA48154A1 (en) 2021-09-30
MA48154B1 (en) 2022-05-31
AR118606A2 (en) 2021-10-20
MA39398A1 (en) 2019-03-29
MA59673A1 (en) 2023-04-28

Similar Documents

Publication Publication Date Title
CL2018001213A1 (en) Oligonocleotide compositions and methods to perform them (sol divisional. No. 2749-16)
BR112014009066B8 (en) nucleic acid molecule, method for synthesizing a tricyclic phosphorothioate dna molecule and pharmaceutical composition
EA201490993A1 (en) MODIFIED MEANS OF RNA
ECSP14010277A (en) SUBSTITUTE NUCLEOSIDES, NUCLEOTIDES AND ANALOGUES THEREOF
CL2013002990A1 (en) Compounds derived from imidazo [5,1-a] isoindole or their salts, inhibitors of indolamine 2,3-dioxygenase (gone); pharmaceutical composition, useful for treating immunosuppression associated with an infectious disease or cancer.
CR20140232A (en) IARN AGENTS, COMPOSITIONS AND METHODS OF USING THE SAME TO TREAT DISEASES ASSOCIATED WITH TRANSTIRETINE (TTR)
ES2871036T3 (en) Method for treating bile acid-related disorders
EA201690274A1 (en) METHOD OF SITE-SPECIFIC ENZYMATIC SWIPING OF NUCLEIC ACIDS IN VITRO INTRODUCTION NOT MEETING IN THE NATURE OF NUCLEOTIDES
ECSP11011391A (en) METHODS AND INTERMEDIATE PRODUCTS TO PREPARE PHARMACEUTICAL AGENTS
UY29408A1 (en) QUICK RELEASE MICROCAKE PRODUCTS.
CL2013000445A1 (en) Oligonucleotide chelate comprising two or more oligonucleotides linked by a multivalent cation where at least one oligonucleotide of said complex has at least one phosphorothioate bond; pharmaceutical composition that includes it; and its uses
CO6670583A2 (en) Process to prepare antiviral compounds
CY1121304T1 (en) METHOD OF CLEANING SOAPS
AR100946A1 (en) COMPOSITIONS AND METHODS TO REGULATE GENE EXPRESSION THROUGH RNA INTERFERENCE
PE20141155A1 (en) ANALOGS OF EPOXY-EICOSATRIENOIC ACID AND METHODS FOR MAKING AND USING THE SAME
AR070326A1 (en) VECTORS OF CLOSTEROVIRUS AND RELATED METHODS
BR112012003802A2 (en) method for removing a non-lipid enveloped virus from a solution.
ECSP13013094A (en) COLINA SALT CLEANING COMPOSITIONS
UY31871A (en) COMPOUNDS THAT INCLUDE A CICLOBUTOXI GROUP
CR20110524A (en) PROCESS TO PREPARE ANTIVIRAL COMPOUNDS
AR100235A1 (en) COMPOSITIONS OF OLIGONUCLEOTIDES AND METHODS TO PERFORM THEM
WO2013033705A3 (en) Non-natural nucleosides as theranostic agents
EA202090282A1 (en) COMPOSITIONS OF OLIGONUCLEOTIDES AND METHODS FOR PRODUCING THEM
AR105066A1 (en) ENZYMES AND CRISPR SYSTEMS
TH166778A (en) Oligon nucleotide compositions and methods for making them