MA48154B1 - Oligonucleotide compositions and methods for their preparation - Google Patents
Oligonucleotide compositions and methods for their preparationInfo
- Publication number
- MA48154B1 MA48154B1 MA48154A MA48154A MA48154B1 MA 48154 B1 MA48154 B1 MA 48154B1 MA 48154 A MA48154 A MA 48154A MA 48154 A MA48154 A MA 48154A MA 48154 B1 MA48154 B1 MA 48154B1
- Authority
- MA
- Morocco
- Prior art keywords
- free
- amino group
- dinucleotide
- protected
- oligonucleotide
- Prior art date
Links
Abstract
La présente divulgation concerne un procédé en phase solide pour préparer des oligonucléotides par l'intermédiaire de cycles de couplages séquentiels comprenant au moins un couplage d'un sous-motif dimère dinucléotidique à un groupe 3'-terminal libre d'une chaîne en cours d'allongement. Les oligonucléotides comprennent au moins deux sous-motifs nucléosidiques reliés par une liaison n3'? P5'phosphoramidate. Le procédé peut comprendre les étapes de (a) déprotection du groupe 3'-amino protégé d'un nucléoside terminal immobilisé sur un support en phase solide, ladite déprotection formant un groupe 3'-amino libre ; (b) mise en contact du groupe 3'-amino libre avec un dimère 3'-amino protégé-dinucléotide-5'-phosphoramidite en présence d'un catalyseur nucléophile pour former une liaison internucléosidique n3'? P5' ; et (c) oxydation (p. Ex., sulfuration) de la liaison. Les compositions produites par les présents procédés peuvent contenir une quantité réduite d'un ou de plusieurs produits oligonucléotidiques (n-x). Des compositions pharmaceutiques contenant les présentes compositions d'oligonucléotides sont en outre décrites.The present disclosure relates to a solid-phase method for preparing oligonucleotides via sequential coupling cycles comprising at least one coupling of a dinucleotide dimer subunit to a free 3'-terminal group of a chain in process of 'elongation. The oligonucleotides comprise at least two nucleoside subunits linked by an n3'? P5'phosphoramidate. The method may comprise the steps of (a) deprotecting the protected 3'-amino group from a terminal nucleoside immobilized on a solid phase support, said deprotection forming a free 3'-amino group; (b) contacting the free 3'-amino group with a 3'-protected amino-dinucleotide-5'-phosphoramidite dimer in the presence of a nucleophilic catalyst to form an internucleoside n3'? P5'; and (c) oxidation (eg, sulfurization) of the bond. The compositions produced by the present methods may contain a reduced amount of one or more oligonucleotide products (n-x). Pharmaceutical compositions containing the present oligonucleotide compositions are further described.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201461987396P | 2014-05-01 | 2014-05-01 | |
PCT/US2015/028327 WO2015168310A1 (en) | 2014-05-01 | 2015-04-29 | Oligonucleotide compositions and methods of making the same |
Publications (2)
Publication Number | Publication Date |
---|---|
MA48154A1 MA48154A1 (en) | 2021-09-30 |
MA48154B1 true MA48154B1 (en) | 2022-05-31 |
Family
ID=58700032
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MA39398A MA39398A1 (en) | 2014-05-01 | 2015-04-29 | Oligonucleotide compositions and methods for their preparation |
MA48154A MA48154B1 (en) | 2014-05-01 | 2015-04-29 | Oligonucleotide compositions and methods for their preparation |
MA59673A MA59673A1 (en) | 2014-05-01 | 2016-10-20 | Oligonucleotide compositions and methods of preparing them |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MA39398A MA39398A1 (en) | 2014-05-01 | 2015-04-29 | Oligonucleotide compositions and methods for their preparation |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MA59673A MA59673A1 (en) | 2014-05-01 | 2016-10-20 | Oligonucleotide compositions and methods of preparing them |
Country Status (2)
Country | Link |
---|---|
AR (2) | AR100235A1 (en) |
MA (3) | MA39398A1 (en) |
-
2015
- 2015-04-29 MA MA39398A patent/MA39398A1/en unknown
- 2015-04-29 AR ARP150101301A patent/AR100235A1/en unknown
- 2015-04-29 MA MA48154A patent/MA48154B1/en unknown
-
2016
- 2016-10-20 MA MA59673A patent/MA59673A1/en unknown
-
2020
- 2020-04-06 AR ARP200100967A patent/AR118606A2/en unknown
Also Published As
Publication number | Publication date |
---|---|
MA59673A1 (en) | 2023-04-28 |
MA48154A1 (en) | 2021-09-30 |
MA39398A1 (en) | 2019-03-29 |
AR100235A1 (en) | 2016-09-21 |
AR118606A2 (en) | 2021-10-20 |
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