MA48154B1 - Oligonucleotide compositions and methods for their preparation - Google Patents

Oligonucleotide compositions and methods for their preparation

Info

Publication number
MA48154B1
MA48154B1 MA48154A MA48154A MA48154B1 MA 48154 B1 MA48154 B1 MA 48154B1 MA 48154 A MA48154 A MA 48154A MA 48154 A MA48154 A MA 48154A MA 48154 B1 MA48154 B1 MA 48154B1
Authority
MA
Morocco
Prior art keywords
free
amino group
dinucleotide
protected
oligonucleotide
Prior art date
Application number
MA48154A
Other languages
French (fr)
Other versions
MA48154A1 (en
Inventor
Premchandran H Ramiya
Original Assignee
Geron Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Geron Corp filed Critical Geron Corp
Priority claimed from PCT/US2015/028327 external-priority patent/WO2015168310A1/en
Publication of MA48154A1 publication Critical patent/MA48154A1/en
Publication of MA48154B1 publication Critical patent/MA48154B1/en

Links

Abstract

La présente divulgation concerne un procédé en phase solide pour préparer des oligonucléotides par l'intermédiaire de cycles de couplages séquentiels comprenant au moins un couplage d'un sous-motif dimère dinucléotidique à un groupe 3'-terminal libre d'une chaîne en cours d'allongement. Les oligonucléotides comprennent au moins deux sous-motifs nucléosidiques reliés par une liaison n3'? P5'phosphoramidate. Le procédé peut comprendre les étapes de (a) déprotection du groupe 3'-amino protégé d'un nucléoside terminal immobilisé sur un support en phase solide, ladite déprotection formant un groupe 3'-amino libre ; (b) mise en contact du groupe 3'-amino libre avec un dimère 3'-amino protégé-dinucléotide-5'-phosphoramidite en présence d'un catalyseur nucléophile pour former une liaison internucléosidique n3'? P5' ; et (c) oxydation (p. Ex., sulfuration) de la liaison. Les compositions produites par les présents procédés peuvent contenir une quantité réduite d'un ou de plusieurs produits oligonucléotidiques (n-x). Des compositions pharmaceutiques contenant les présentes compositions d'oligonucléotides sont en outre décrites.The present disclosure relates to a solid-phase method for preparing oligonucleotides via sequential coupling cycles comprising at least one coupling of a dinucleotide dimer subunit to a free 3'-terminal group of a chain in process of 'elongation. The oligonucleotides comprise at least two nucleoside subunits linked by an n3'? P5'phosphoramidate. The method may comprise the steps of (a) deprotecting the protected 3'-amino group from a terminal nucleoside immobilized on a solid phase support, said deprotection forming a free 3'-amino group; (b) contacting the free 3'-amino group with a 3'-protected amino-dinucleotide-5'-phosphoramidite dimer in the presence of a nucleophilic catalyst to form an internucleoside n3'? P5'; and (c) oxidation (eg, sulfurization) of the bond. The compositions produced by the present methods may contain a reduced amount of one or more oligonucleotide products (n-x). Pharmaceutical compositions containing the present oligonucleotide compositions are further described.

MA48154A 2014-05-01 2015-04-29 Oligonucleotide compositions and methods for their preparation MA48154B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461987396P 2014-05-01 2014-05-01
PCT/US2015/028327 WO2015168310A1 (en) 2014-05-01 2015-04-29 Oligonucleotide compositions and methods of making the same

Publications (2)

Publication Number Publication Date
MA48154A1 MA48154A1 (en) 2021-09-30
MA48154B1 true MA48154B1 (en) 2022-05-31

Family

ID=58700032

Family Applications (3)

Application Number Title Priority Date Filing Date
MA39398A MA39398A1 (en) 2014-05-01 2015-04-29 Oligonucleotide compositions and methods for their preparation
MA48154A MA48154B1 (en) 2014-05-01 2015-04-29 Oligonucleotide compositions and methods for their preparation
MA59673A MA59673A1 (en) 2014-05-01 2016-10-20 Oligonucleotide compositions and methods of preparing them

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MA39398A MA39398A1 (en) 2014-05-01 2015-04-29 Oligonucleotide compositions and methods for their preparation

Family Applications After (1)

Application Number Title Priority Date Filing Date
MA59673A MA59673A1 (en) 2014-05-01 2016-10-20 Oligonucleotide compositions and methods of preparing them

Country Status (2)

Country Link
AR (2) AR100235A1 (en)
MA (3) MA39398A1 (en)

Also Published As

Publication number Publication date
MA59673A1 (en) 2023-04-28
MA48154A1 (en) 2021-09-30
MA39398A1 (en) 2019-03-29
AR100235A1 (en) 2016-09-21
AR118606A2 (en) 2021-10-20

Similar Documents

Publication Publication Date Title
MX2021004932A (en) Oligonucleotide compositions and methods of making the same.
EA201690274A1 (en) METHOD OF SITE-SPECIFIC ENZYMATIC SWIPING OF NUCLEIC ACIDS IN VITRO INTRODUCTION NOT MEETING IN THE NATURE OF NUCLEOTIDES
BR112020015093A2 (en) DIAGNOSIS BASED ON THE CRISPR EFFECTOR SYSTEM
PE20211498A1 (en) ANTIBODIES DIRECTED AGAINST IL-11
WO2008069906A3 (en) Digital expression of gene analysis
MX340330B (en) Method and product for localised or spatial detection of nucleic acid in a tissue sample.
WO2016160844A3 (en) Methods and compositions for combinatorial barcoding
DE602006012971D1 (en) MEDIATED CHEMISTRY
EP3351631A3 (en) Methods for tagging dna-encoded libraries
WO2012142611A3 (en) Sequencing small amounts of complex nucleic acids
WO2008080029A3 (en) Methods and compositions for nucleic acid amplification
MX342764B (en) Compositions and methods for selective delivery of oligonucleotide molecules to specific neuron types.
WO2016161375A3 (en) Methods of using oligonucleotide-guided argonaute proteins
ATE548454T1 (en) NUCLEIC ACID ACTIVE SUBSTANCES FOR STOPPING H19 FOR THE TREATMENT OF RHEUMATOID ARTHRITIS
WO2008073960A3 (en) Oligonucleotides containing high concentrations of guanine monomers
MY196912A (en) Short pendant arm linkers for nucleotides in sequencing applications
KR20220080136A (en) Compositions and Methods for In Vivo Synthesis of Non-Natural Polypeptides
PE20091969A1 (en) ANTI-PirB ANTIBODIES
MA59673A1 (en) Oligonucleotide compositions and methods of preparing them
RU2009128762A (en) METHOD AND SET FOR IMMUNO-ENZYME DETERMINATION OF FUNCTIONAL ACTIVITY OF HUMAN COMPLEMENT COMPONENT C3
WO2011067450A8 (en) Covalently biofunctionalized supports
EA202090282A1 (en) COMPOSITIONS OF OLIGONUCLEOTIDES AND METHODS FOR PRODUCING THEM
MX343244B (en) Method for the detection of colletotrichum capsici phytopathogen fungi using the polymerase chain reaction (pcr).
RU2013107428A (en) METHOD FOR DIFFERENTIATION OF THE CIRCUIT OF SIBERIAN ANALYSIS FROM OTHER CLOSE TYPES OF THE GENUS BACILLUS ON THE BASIS OF DETERMINATION DIFFERENT IN THE STRUCTURE OF CHROMOSOMIC GENES
MX2014009935A (en) Functionalization process by means of mercaptohexanol for the covalent anchoring of dna over gold with potential implementation for the development of biosensors.