MA48154A1 - Oligonucleotide compositions and their preparation methods - Google Patents

Oligonucleotide compositions and their preparation methods

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Publication number
MA48154A1
MA48154A1 MA48154A MA48154A MA48154A1 MA 48154 A1 MA48154 A1 MA 48154A1 MA 48154 A MA48154 A MA 48154A MA 48154 A MA48154 A MA 48154A MA 48154 A1 MA48154 A1 MA 48154A1
Authority
MA
Morocco
Prior art keywords
free
bond
amino group
dinucleotide
protected
Prior art date
Application number
MA48154A
Other languages
French (fr)
Other versions
MA48154B1 (en
Inventor
Premchandran H Ramiya
Original Assignee
Geron Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Geron Corp filed Critical Geron Corp
Priority claimed from PCT/US2015/028327 external-priority patent/WO2015168310A1/en
Publication of MA48154A1 publication Critical patent/MA48154A1/en
Publication of MA48154B1 publication Critical patent/MA48154B1/en

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  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)
  • Medicinal Preparation (AREA)

Abstract

La présente divulgation concerne un procédé en phase solide pour préparer des oligonucléotides par l'intermédiaire de cycles de couplages séquentiels comprenant au moins un couplage d'un sous-motif dimère dinucléotidique à un groupe 3'-terminal libre d'une chaîne en cours d'allongement. Les oligonucléotides comprennent au moins deux sous-motifs nucléosidiques reliés par une liaison n3'? P5'phosphoramidate. Le procédé peut comprendre les étapes de (a) déprotection du groupe 3'-amino protégé d'un nucléoside terminal immobilisé sur un support en phase solide, ladite déprotection formant un groupe 3'-amino libre ; (b) mise en contact du groupe 3'-amino libre avec un dimère 3'-amino protégé-dinucléotide-5'-phosphoramidite en présence d'un catalyseur nucléophile pour former une liaison internucléosidique n3'? P5' ; et (c) oxydation (p. Ex., sulfuration) de la liaison. Les compositions produites par les présents procédés peuvent contenir une quantité réduite d'un ou de plusieurs produits oligonucléotidiques (n-x). Des compositions pharmaceutiques contenant les présentes compositions d'oligonucléotides sont en outre décrites.The present disclosure relates to a solid phase process for preparing oligonucleotides via rounds of sequential coupling comprising at least one coupling of a dinucleotide dimeric subunit to a free 3'-terminal group of a chain in progress. 'elongation. The oligonucleotides comprise at least two nucleoside subunits linked by an n3 ′ bond. P5'phosphoramidate. The method may comprise the steps of (a) deprotecting the protected 3'-amino group of a terminal nucleoside immobilized on a solid phase support, said deprotection forming a free 3'-amino group; (b) contacting the free 3'-amino group with a protected 3'-amino-dinucleotide-5'-phosphoramidite dimer in the presence of a nucleophilic catalyst to form an n3 'internucleoside bond? P5 '; and (c) oxidation (eg, sulfurization) of the bond. Compositions produced by the present methods may contain a reduced amount of one or more oligonucleotide products (n-x). Pharmaceutical compositions containing the present oligonucleotide compositions are further disclosed.

MA48154A 2014-05-01 2015-04-29 Oligonucleotide compositions and methods for their preparation MA48154B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461987396P 2014-05-01 2014-05-01
PCT/US2015/028327 WO2015168310A1 (en) 2014-05-01 2015-04-29 Oligonucleotide compositions and methods of making the same

Publications (2)

Publication Number Publication Date
MA48154A1 true MA48154A1 (en) 2021-09-30
MA48154B1 MA48154B1 (en) 2022-05-31

Family

ID=58700032

Family Applications (3)

Application Number Title Priority Date Filing Date
MA39398A MA39398A1 (en) 2014-05-01 2015-04-29 Oligonucleotide compositions and methods for their preparation
MA48154A MA48154B1 (en) 2014-05-01 2015-04-29 Oligonucleotide compositions and methods for their preparation
MA59673A MA59673A1 (en) 2014-05-01 2016-10-20 Oligonucleotide compositions and methods of preparing them

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MA39398A MA39398A1 (en) 2014-05-01 2015-04-29 Oligonucleotide compositions and methods for their preparation

Family Applications After (1)

Application Number Title Priority Date Filing Date
MA59673A MA59673A1 (en) 2014-05-01 2016-10-20 Oligonucleotide compositions and methods of preparing them

Country Status (2)

Country Link
AR (2) AR100235A1 (en)
MA (3) MA39398A1 (en)

Also Published As

Publication number Publication date
MA59673A1 (en) 2023-04-28
MA48154B1 (en) 2022-05-31
MA39398A1 (en) 2019-03-29
AR100235A1 (en) 2016-09-21
AR118606A2 (en) 2021-10-20

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