MX2010012061A - Method for the detection of colletotrichum capsici phytopathogen fungi using the polymerase chain reaction (pcr). - Google Patents
Method for the detection of colletotrichum capsici phytopathogen fungi using the polymerase chain reaction (pcr).Info
- Publication number
- MX2010012061A MX2010012061A MX2010012061A MX2010012061A MX2010012061A MX 2010012061 A MX2010012061 A MX 2010012061A MX 2010012061 A MX2010012061 A MX 2010012061A MX 2010012061 A MX2010012061 A MX 2010012061A MX 2010012061 A MX2010012061 A MX 2010012061A
- Authority
- MX
- Mexico
- Prior art keywords
- chain reaction
- polymerase chain
- pcr
- colletotrichum capsici
- detection
- Prior art date
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Described are initiators, a detection kit and method for detecting Colletotrichum capsici, which comprises the following steps: (a) obtaining a biological sample from the desired plant; (b) isolating the DNA of said biological sample; (c) subjecting the DNA to a polymerase chain reaction (PCR) using as initiator a sense sequence (SEQ ID NO. 1): 5'-GTA GCC GTC CCC TAA AAA GG-3' and an antisense sequence ((SEQ ID NO. 2): 5'-CCC AAT GCG AGA CGA AAT G-3'; and (d) detecting the Colletotrichum capsici by visualizing the amplified product of the polymerase chain reaction.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MX2010012061A MX343244B (en) | 2010-11-04 | 2010-11-04 | Method for the detection of colletotrichum capsici phytopathogen fungi using the polymerase chain reaction (pcr). |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MX2010012061A MX343244B (en) | 2010-11-04 | 2010-11-04 | Method for the detection of colletotrichum capsici phytopathogen fungi using the polymerase chain reaction (pcr). |
Publications (2)
Publication Number | Publication Date |
---|---|
MX2010012061A true MX2010012061A (en) | 2012-05-17 |
MX343244B MX343244B (en) | 2016-10-21 |
Family
ID=46511913
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2010012061A MX343244B (en) | 2010-11-04 | 2010-11-04 | Method for the detection of colletotrichum capsici phytopathogen fungi using the polymerase chain reaction (pcr). |
Country Status (1)
Country | Link |
---|---|
MX (1) | MX343244B (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114540468A (en) * | 2021-12-22 | 2022-05-27 | 海南大学 | Method for quantitatively detecting colletotrichum gloeosporioides by using real-time fluorescent PCR (polymerase chain reaction) |
-
2010
- 2010-11-04 MX MX2010012061A patent/MX343244B/en active IP Right Grant
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114540468A (en) * | 2021-12-22 | 2022-05-27 | 海南大学 | Method for quantitatively detecting colletotrichum gloeosporioides by using real-time fluorescent PCR (polymerase chain reaction) |
Also Published As
Publication number | Publication date |
---|---|
MX343244B (en) | 2016-10-21 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG | Grant or registration |