MX2010012061A - Method for the detection of colletotrichum capsici phytopathogen fungi using the polymerase chain reaction (pcr). - Google Patents

Method for the detection of colletotrichum capsici phytopathogen fungi using the polymerase chain reaction (pcr).

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Publication number
MX2010012061A
MX2010012061A MX2010012061A MX2010012061A MX2010012061A MX 2010012061 A MX2010012061 A MX 2010012061A MX 2010012061 A MX2010012061 A MX 2010012061A MX 2010012061 A MX2010012061 A MX 2010012061A MX 2010012061 A MX2010012061 A MX 2010012061A
Authority
MX
Mexico
Prior art keywords
chain reaction
polymerase chain
pcr
colletotrichum capsici
detection
Prior art date
Application number
MX2010012061A
Other languages
Spanish (es)
Other versions
MX343244B (en
Inventor
Claudia Guadalupe Torres Calzada
Raul Tapia Tussell
Daisy De La Caridad Perez Brito
Andres Felipe De Jesus Quijano Ramayo
Inocencio Higuera Ciapara
Original Assignee
Ct De Investigacion Cientifica De Yucatan A C
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ct De Investigacion Cientifica De Yucatan A C filed Critical Ct De Investigacion Cientifica De Yucatan A C
Priority to MX2010012061A priority Critical patent/MX343244B/en
Publication of MX2010012061A publication Critical patent/MX2010012061A/en
Publication of MX343244B publication Critical patent/MX343244B/en

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Abstract

Described are initiators, a detection kit and method for detecting Colletotrichum capsici, which comprises the following steps: (a) obtaining a biological sample from the desired plant; (b) isolating the DNA of said biological sample; (c) subjecting the DNA to a polymerase chain reaction (PCR) using as initiator a sense sequence (SEQ ID NO. 1): 5'-GTA GCC GTC CCC TAA AAA GG-3' and an antisense sequence ((SEQ ID NO. 2): 5'-CCC AAT GCG AGA CGA AAT G-3'; and (d) detecting the Colletotrichum capsici by visualizing the amplified product of the polymerase chain reaction.
MX2010012061A 2010-11-04 2010-11-04 Method for the detection of colletotrichum capsici phytopathogen fungi using the polymerase chain reaction (pcr). MX343244B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
MX2010012061A MX343244B (en) 2010-11-04 2010-11-04 Method for the detection of colletotrichum capsici phytopathogen fungi using the polymerase chain reaction (pcr).

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MX2010012061A MX343244B (en) 2010-11-04 2010-11-04 Method for the detection of colletotrichum capsici phytopathogen fungi using the polymerase chain reaction (pcr).

Publications (2)

Publication Number Publication Date
MX2010012061A true MX2010012061A (en) 2012-05-17
MX343244B MX343244B (en) 2016-10-21

Family

ID=46511913

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2010012061A MX343244B (en) 2010-11-04 2010-11-04 Method for the detection of colletotrichum capsici phytopathogen fungi using the polymerase chain reaction (pcr).

Country Status (1)

Country Link
MX (1) MX343244B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114540468A (en) * 2021-12-22 2022-05-27 海南大学 Method for quantitatively detecting colletotrichum gloeosporioides by using real-time fluorescent PCR (polymerase chain reaction)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114540468A (en) * 2021-12-22 2022-05-27 海南大学 Method for quantitatively detecting colletotrichum gloeosporioides by using real-time fluorescent PCR (polymerase chain reaction)

Also Published As

Publication number Publication date
MX343244B (en) 2016-10-21

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