AR097977A1 - L-AMINO ACIDS PRODUCTION METHOD - Google Patents
L-AMINO ACIDS PRODUCTION METHODInfo
- Publication number
- AR097977A1 AR097977A1 ARP140103766A ARP140103766A AR097977A1 AR 097977 A1 AR097977 A1 AR 097977A1 AR P140103766 A ARP140103766 A AR P140103766A AR P140103766 A ARP140103766 A AR P140103766A AR 097977 A1 AR097977 A1 AR 097977A1
- Authority
- AR
- Argentina
- Prior art keywords
- amino acids
- producing
- target gene
- recombinant coryneform
- coryneform microorganism
- Prior art date
Links
- 150000008575 L-amino acids Chemical class 0.000 title abstract 5
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 abstract 4
- 244000005700 microbiome Species 0.000 abstract 4
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- 108020004705 Codon Proteins 0.000 abstract 1
- 150000001242 acetic acid derivatives Chemical class 0.000 abstract 1
- 210000000349 chromosome Anatomy 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 230000005764 inhibitory process Effects 0.000 abstract 1
- 238000003780 insertion Methods 0.000 abstract 1
- 230000037431 insertion Effects 0.000 abstract 1
- 238000013518 transcription Methods 0.000 abstract 1
- 230000035897 transcription Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y403/00—Carbon-nitrogen lyases (4.3)
- C12Y403/03—Amine-lyases (4.3.3)
- C12Y403/03007—4-Hydroxy-tetrahydrodipicolinate synthase (4.3.3.7)
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Se proporciona un método de producción de L-aminoácidos mediante el uso de un microorganismo corineforme recombinante en el que la expresión de un gen objetivo es debilitada mediante el uso de un método de inhibición de la transcripción génica. Reivindicación 1: Un método de producción de L-aminoácidos, comprendiendo el método 1) cultivar un microorganismo corineforme recombinante capaz de producir L-aminoácidos, en donde el microorganismo corineforme recombinante se transforma mediante inserción de un promotor inducible por acetato corriente abajo de un codón de terminación de un gen objetivo en un cromosoma; y 2) agregar acetatos durante el cultivo para debilitar la expresión del gen objetivo y para fortalecer la capacidad de producción de L-aminoácidos del microorganismo corineforme recombinante.A method of producing L-amino acids is provided by the use of a recombinant coryneform microorganism in which the expression of a target gene is weakened by the use of a method of inhibition of gene transcription. Claim 1: A method of producing L-amino acids, method 1) comprising culturing a recombinant coryneform microorganism capable of producing L-amino acids, wherein the recombinant coryneform microorganism is transformed by insertion of an acetate-inducible promoter downstream of a codon termination of a target gene on a chromosome; and 2) add acetates during culture to weaken the expression of the target gene and to strengthen the production capacity of L-amino acids of the recombinant coryneform microorganism.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR20130121090 | 2013-10-11 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR097977A1 true AR097977A1 (en) | 2016-04-20 |
Family
ID=53035683
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP140103766A AR097977A1 (en) | 2013-10-11 | 2014-10-09 | L-AMINO ACIDS PRODUCTION METHOD |
Country Status (16)
Country | Link |
---|---|
JP (3) | JP6291042B2 (en) |
KR (1) | KR101518860B1 (en) |
CN (1) | CN104561163B (en) |
AR (1) | AR097977A1 (en) |
AU (2) | AU2014332732B2 (en) |
BR (1) | BR112016008029A2 (en) |
CA (1) | CA2925659C (en) |
DK (1) | DK2860256T3 (en) |
ES (1) | ES2626048T3 (en) |
GE (1) | GEP20196943B (en) |
JO (1) | JO3255B1 (en) |
MY (1) | MY172463A (en) |
PH (1) | PH12016500640B1 (en) |
PL (1) | PL2860256T3 (en) |
RU (2) | RU2651461C2 (en) |
UA (1) | UA117935C2 (en) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101518860B1 (en) * | 2013-10-11 | 2015-05-12 | 씨제이제일제당 (주) | Method for producing L-amino acid |
CN105734004B (en) * | 2016-03-02 | 2020-06-19 | 廊坊梅花生物技术开发有限公司 | Recombinant strain and preparation method and application thereof |
KR101925930B1 (en) * | 2017-07-24 | 2019-02-26 | 씨제이제일제당 주식회사 | Microorganisms producing L-lysine and process for producing L-lysine using the same |
CA3073607C (en) | 2018-03-09 | 2023-07-04 | Cj Cheiljedang Corporation | A novel promoter and a method for producing l-amino acid using the same |
KR102221040B1 (en) * | 2019-05-09 | 2021-03-03 | 씨제이제일제당 주식회사 | Microorganism producing L-amino acid and method of producing Method of L-amino acid using thereof |
KR102134418B1 (en) * | 2019-06-17 | 2020-07-16 | 씨제이제일제당 주식회사 | A microorganism producing L-tyrosine and a method for producing L-tyrosine using thereof |
KR102207867B1 (en) * | 2020-01-21 | 2021-01-26 | 씨제이제일제당 주식회사 | Method for producing L-amino acids using microorganisms containing NADP dependent glyceraldehyde-3-phosphate dehydrogenase |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6649379B1 (en) * | 1987-06-12 | 2003-11-18 | Massachusetts Institute Of Technology | Method and deregulated enzyme for threonine production |
JP3036912B2 (en) * | 1991-09-02 | 2000-04-24 | 協和醗酵工業株式会社 | Gene expression regulatory DNA |
DK0754756T3 (en) * | 1994-03-04 | 2006-03-13 | Ajinomoto Kk | Process for Preparation of L-Lysine |
CZ293268B6 (en) * | 1995-06-07 | 2004-03-17 | Ajinomoto Co., Inc. | Recombinant DNA, bacterium and process for producing L-lysine |
ES2218009T3 (en) * | 1999-02-20 | 2004-11-16 | Degussa Ag | PROCEDURE FOR THE FERMENTATIVE PREPARATION OF L-AMINO ACIDS USING CORINEFORM BACTERIA. |
WO2001048146A1 (en) * | 1999-12-24 | 2001-07-05 | Ajinomoto Co., Inc. | Process for producing l-amino acid and novel gene |
WO2002040679A2 (en) * | 2000-11-15 | 2002-05-23 | Archer-Daniels-Midland Company | Corynebacterium glutamicum promoters |
KR100397423B1 (en) * | 2001-02-13 | 2003-09-13 | 씨제이 주식회사 | Process for producing L-threonine |
DE10224088A1 (en) * | 2002-05-31 | 2003-12-11 | Degussa | Process for the production of L-amino acids using coryneform bacteria which contain an attenuated mez gene |
JP2004261150A (en) * | 2003-03-04 | 2004-09-24 | Ajinomoto Co Inc | Method for producing objective material |
JP2005006564A (en) | 2003-06-19 | 2005-01-13 | Japan Tobacco Inc | Nitrate ion-inducing promoter |
JP2010226956A (en) * | 2007-07-23 | 2010-10-14 | Ajinomoto Co Inc | Method for producing l-lysine |
KR101335853B1 (en) | 2011-12-01 | 2013-12-02 | 씨제이제일제당 (주) | A microorganism having L-amino acids and riboflavin productivity and a method of producing L-amino acids and riboflavin using the same |
KR101518860B1 (en) * | 2013-10-11 | 2015-05-12 | 씨제이제일제당 (주) | Method for producing L-amino acid |
-
2014
- 2014-07-18 KR KR1020140091307A patent/KR101518860B1/en active IP Right Grant
- 2014-08-10 UA UAA201603717A patent/UA117935C2/en unknown
- 2014-09-24 PL PL14186238T patent/PL2860256T3/en unknown
- 2014-09-24 DK DK14186238.3T patent/DK2860256T3/en active
- 2014-09-24 ES ES14186238.3T patent/ES2626048T3/en active Active
- 2014-10-08 AU AU2014332732A patent/AU2014332732B2/en active Active
- 2014-10-08 MY MYPI2016701087A patent/MY172463A/en unknown
- 2014-10-08 CA CA2925659A patent/CA2925659C/en active Active
- 2014-10-08 GE GEAP201414125A patent/GEP20196943B/en unknown
- 2014-10-08 RU RU2016110804A patent/RU2651461C2/en active
- 2014-10-08 RU RU2015151124A patent/RU2614258C2/en active
- 2014-10-08 JP JP2016521791A patent/JP6291042B2/en active Active
- 2014-10-08 BR BR112016008029A patent/BR112016008029A2/en not_active Application Discontinuation
- 2014-10-09 JO JOP/2014/0287A patent/JO3255B1/en active
- 2014-10-09 AR ARP140103766A patent/AR097977A1/en unknown
- 2014-10-11 CN CN201410654216.XA patent/CN104561163B/en active Active
-
2016
- 2016-04-07 PH PH12016500640A patent/PH12016500640B1/en unknown
-
2017
- 2017-12-07 AU AU2017272295A patent/AU2017272295B2/en active Active
-
2018
- 2018-02-08 JP JP2018021175A patent/JP6824918B2/en active Active
-
2020
- 2020-01-24 JP JP2020009563A patent/JP2020078312A/en not_active Withdrawn
Also Published As
Publication number | Publication date |
---|---|
DK2860256T3 (en) | 2017-06-06 |
KR20150042692A (en) | 2015-04-21 |
AU2017272295B2 (en) | 2019-05-16 |
UA117935C2 (en) | 2018-10-25 |
JP6824918B2 (en) | 2021-02-03 |
MY172463A (en) | 2019-11-26 |
CA2925659C (en) | 2018-10-23 |
RU2015151124A (en) | 2016-06-27 |
PH12016500640A1 (en) | 2016-05-30 |
JO3255B1 (en) | 2018-09-16 |
CN104561163B (en) | 2018-11-06 |
JP2020078312A (en) | 2020-05-28 |
ES2626048T3 (en) | 2017-07-21 |
RU2651461C2 (en) | 2018-04-19 |
RU2614258C2 (en) | 2017-03-24 |
CN104561163A (en) | 2015-04-29 |
AU2014332732B2 (en) | 2017-12-07 |
KR101518860B1 (en) | 2015-05-12 |
JP2018113966A (en) | 2018-07-26 |
JP6291042B2 (en) | 2018-03-14 |
RU2016110804A (en) | 2016-07-27 |
GEP20196943B (en) | 2019-02-11 |
AU2017272295A1 (en) | 2018-01-04 |
JP2016532439A (en) | 2016-10-20 |
AU2014332732A1 (en) | 2016-04-21 |
CA2925659A1 (en) | 2015-04-16 |
BR112016008029A2 (en) | 2017-10-03 |
PH12016500640B1 (en) | 2016-05-30 |
PL2860256T3 (en) | 2017-08-31 |
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