AR097977A1 - L-AMINO ACIDS PRODUCTION METHOD - Google Patents

L-AMINO ACIDS PRODUCTION METHOD

Info

Publication number
AR097977A1
AR097977A1 ARP140103766A ARP140103766A AR097977A1 AR 097977 A1 AR097977 A1 AR 097977A1 AR P140103766 A ARP140103766 A AR P140103766A AR P140103766 A ARP140103766 A AR P140103766A AR 097977 A1 AR097977 A1 AR 097977A1
Authority
AR
Argentina
Prior art keywords
amino acids
producing
target gene
recombinant coryneform
coryneform microorganism
Prior art date
Application number
ARP140103766A
Other languages
Spanish (es)
Inventor
Jo Lim Sang
Won Bae Hyun
Hyun Kwon Do
Ho Lee Kwang
Ok Moon Jun
Original Assignee
Cj Cheiljedang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cj Cheiljedang Corp filed Critical Cj Cheiljedang Corp
Publication of AR097977A1 publication Critical patent/AR097977A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y403/00—Carbon-nitrogen lyases (4.3)
    • C12Y403/03—Amine-lyases (4.3.3)
    • C12Y403/03007—4-Hydroxy-tetrahydrodipicolinate synthase (4.3.3.7)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Se proporciona un método de producción de L-aminoácidos mediante el uso de un microorganismo corineforme recombinante en el que la expresión de un gen objetivo es debilitada mediante el uso de un método de inhibición de la transcripción génica. Reivindicación 1: Un método de producción de L-aminoácidos, comprendiendo el método 1) cultivar un microorganismo corineforme recombinante capaz de producir L-aminoácidos, en donde el microorganismo corineforme recombinante se transforma mediante inserción de un promotor inducible por acetato corriente abajo de un codón de terminación de un gen objetivo en un cromosoma; y 2) agregar acetatos durante el cultivo para debilitar la expresión del gen objetivo y para fortalecer la capacidad de producción de L-aminoácidos del microorganismo corineforme recombinante.A method of producing L-amino acids is provided by the use of a recombinant coryneform microorganism in which the expression of a target gene is weakened by the use of a method of inhibition of gene transcription. Claim 1: A method of producing L-amino acids, method 1) comprising culturing a recombinant coryneform microorganism capable of producing L-amino acids, wherein the recombinant coryneform microorganism is transformed by insertion of an acetate-inducible promoter downstream of a codon termination of a target gene on a chromosome; and 2) add acetates during culture to weaken the expression of the target gene and to strengthen the production capacity of L-amino acids of the recombinant coryneform microorganism.

ARP140103766A 2013-10-11 2014-10-09 L-AMINO ACIDS PRODUCTION METHOD AR097977A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR20130121090 2013-10-11

Publications (1)

Publication Number Publication Date
AR097977A1 true AR097977A1 (en) 2016-04-20

Family

ID=53035683

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP140103766A AR097977A1 (en) 2013-10-11 2014-10-09 L-AMINO ACIDS PRODUCTION METHOD

Country Status (16)

Country Link
JP (3) JP6291042B2 (en)
KR (1) KR101518860B1 (en)
CN (1) CN104561163B (en)
AR (1) AR097977A1 (en)
AU (2) AU2014332732B2 (en)
BR (1) BR112016008029A2 (en)
CA (1) CA2925659C (en)
DK (1) DK2860256T3 (en)
ES (1) ES2626048T3 (en)
GE (1) GEP20196943B (en)
JO (1) JO3255B1 (en)
MY (1) MY172463A (en)
PH (1) PH12016500640B1 (en)
PL (1) PL2860256T3 (en)
RU (2) RU2651461C2 (en)
UA (1) UA117935C2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101518860B1 (en) * 2013-10-11 2015-05-12 씨제이제일제당 (주) Method for producing L-amino acid
CN105734004B (en) * 2016-03-02 2020-06-19 廊坊梅花生物技术开发有限公司 Recombinant strain and preparation method and application thereof
KR101925930B1 (en) * 2017-07-24 2019-02-26 씨제이제일제당 주식회사 Microorganisms producing L-lysine and process for producing L-lysine using the same
CA3073607C (en) 2018-03-09 2023-07-04 Cj Cheiljedang Corporation A novel promoter and a method for producing l-amino acid using the same
KR102221040B1 (en) * 2019-05-09 2021-03-03 씨제이제일제당 주식회사 Microorganism producing L-amino acid and method of producing Method of L-amino acid using thereof
KR102134418B1 (en) * 2019-06-17 2020-07-16 씨제이제일제당 주식회사 A microorganism producing L-tyrosine and a method for producing L-tyrosine using thereof
KR102207867B1 (en) * 2020-01-21 2021-01-26 씨제이제일제당 주식회사 Method for producing L-amino acids using microorganisms containing NADP dependent glyceraldehyde-3-phosphate dehydrogenase

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6649379B1 (en) * 1987-06-12 2003-11-18 Massachusetts Institute Of Technology Method and deregulated enzyme for threonine production
JP3036912B2 (en) * 1991-09-02 2000-04-24 協和醗酵工業株式会社 Gene expression regulatory DNA
DK0754756T3 (en) * 1994-03-04 2006-03-13 Ajinomoto Kk Process for Preparation of L-Lysine
CZ293268B6 (en) * 1995-06-07 2004-03-17 Ajinomoto Co., Inc. Recombinant DNA, bacterium and process for producing L-lysine
ES2218009T3 (en) * 1999-02-20 2004-11-16 Degussa Ag PROCEDURE FOR THE FERMENTATIVE PREPARATION OF L-AMINO ACIDS USING CORINEFORM BACTERIA.
WO2001048146A1 (en) * 1999-12-24 2001-07-05 Ajinomoto Co., Inc. Process for producing l-amino acid and novel gene
WO2002040679A2 (en) * 2000-11-15 2002-05-23 Archer-Daniels-Midland Company Corynebacterium glutamicum promoters
KR100397423B1 (en) * 2001-02-13 2003-09-13 씨제이 주식회사 Process for producing L-threonine
DE10224088A1 (en) * 2002-05-31 2003-12-11 Degussa Process for the production of L-amino acids using coryneform bacteria which contain an attenuated mez gene
JP2004261150A (en) * 2003-03-04 2004-09-24 Ajinomoto Co Inc Method for producing objective material
JP2005006564A (en) 2003-06-19 2005-01-13 Japan Tobacco Inc Nitrate ion-inducing promoter
JP2010226956A (en) * 2007-07-23 2010-10-14 Ajinomoto Co Inc Method for producing l-lysine
KR101335853B1 (en) 2011-12-01 2013-12-02 씨제이제일제당 (주) A microorganism having L-amino acids and riboflavin productivity and a method of producing L-amino acids and riboflavin using the same
KR101518860B1 (en) * 2013-10-11 2015-05-12 씨제이제일제당 (주) Method for producing L-amino acid

Also Published As

Publication number Publication date
DK2860256T3 (en) 2017-06-06
KR20150042692A (en) 2015-04-21
AU2017272295B2 (en) 2019-05-16
UA117935C2 (en) 2018-10-25
JP6824918B2 (en) 2021-02-03
MY172463A (en) 2019-11-26
CA2925659C (en) 2018-10-23
RU2015151124A (en) 2016-06-27
PH12016500640A1 (en) 2016-05-30
JO3255B1 (en) 2018-09-16
CN104561163B (en) 2018-11-06
JP2020078312A (en) 2020-05-28
ES2626048T3 (en) 2017-07-21
RU2651461C2 (en) 2018-04-19
RU2614258C2 (en) 2017-03-24
CN104561163A (en) 2015-04-29
AU2014332732B2 (en) 2017-12-07
KR101518860B1 (en) 2015-05-12
JP2018113966A (en) 2018-07-26
JP6291042B2 (en) 2018-03-14
RU2016110804A (en) 2016-07-27
GEP20196943B (en) 2019-02-11
AU2017272295A1 (en) 2018-01-04
JP2016532439A (en) 2016-10-20
AU2014332732A1 (en) 2016-04-21
CA2925659A1 (en) 2015-04-16
BR112016008029A2 (en) 2017-10-03
PH12016500640B1 (en) 2016-05-30
PL2860256T3 (en) 2017-08-31

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