AR078009A1 - Moleculas de acido nucleico regulatorias para aumentar la expresion genica especifica de semilla y/o preferencial de semilla en las plantas - Google Patents
Moleculas de acido nucleico regulatorias para aumentar la expresion genica especifica de semilla y/o preferencial de semilla en las plantasInfo
- Publication number
- AR078009A1 AR078009A1 ARP100103187A ARP100103187A AR078009A1 AR 078009 A1 AR078009 A1 AR 078009A1 AR P100103187 A ARP100103187 A AR P100103187A AR P100103187 A ARP100103187 A AR P100103187A AR 078009 A1 AR078009 A1 AR 078009A1
- Authority
- AR
- Argentina
- Prior art keywords
- nucleic acid
- acid molecule
- seed
- seq
- sequence
- Prior art date
Links
- 108020004707 nucleic acids Proteins 0.000 title abstract 11
- 150000007523 nucleic acids Chemical class 0.000 title abstract 11
- 102000039446 nucleic acids Human genes 0.000 title abstract 11
- 230000014509 gene expression Effects 0.000 title abstract 4
- 230000001105 regulatory effect Effects 0.000 title 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 abstract 3
- 230000000295 complement effect Effects 0.000 abstract 3
- 239000002773 nucleotide Substances 0.000 abstract 2
- 125000003729 nucleotide group Chemical group 0.000 abstract 2
- 230000009261 transgenic effect Effects 0.000 abstract 2
- 239000003155 DNA primer Substances 0.000 abstract 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 abstract 1
- 241000592344 Spermatophyta Species 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 238000003259 recombinant expression Methods 0.000 abstract 1
- 238000013518 transcription Methods 0.000 abstract 1
- 230000035897 transcription Effects 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/823—Reproductive tissue-specific promoters
- C12N15/8234—Seed-specific, e.g. embryo, endosperm
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Developmental Biology & Embryology (AREA)
- Pregnancy & Childbirth (AREA)
- Reproductive Health (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Se provee además un constructo de expresion recombinante, un vector de expresion, una planta transgénica y una célula transgénica. Reivindicacion 1: Un método para la produccion de un promotor de planta especifico de semilla y/o preferencial de semilla de expresion alta, caracterizado porque comprende la union funcional a un promotor, una o más moléculas de ácido nucleico (NEENA) heterologo que aumentan la expresion del ácido nucleico para dicho promotor que comprende i) la molécula de ácido nucleico que tiene una secuencia que se define en la SEQ ID Ns: 1 a 15, o ii) una molécula de ácido nucleico que tiene una secuencia con una identidad de al menos 80% con la SEQ ID Ns: 1 a 15, o iii) un fragmento de al menos 100 bases consecutivas de una molécula de ácido nucleico de i) o ii) que tiene actividad de aumento de la expresion como la correspondiente molécula de ácido nucleico que tiene la secuencia de SEQ ID Ns:1 a 15, o iv) una molécula de ácido nucleico que es el complemento o complemento inverso de cualquiera de las moléculas de ácido nucleico descriptas previamente bajo i) o ii), o v) una molécula de ácido nucleico que se puede obtener por PCR usando cebadores de oligonucleotido descriptos por la SEQ ID Ns: 20 a 29, 34 a 41, 44 a 51 y 54 a 57 como se muestra en la Tabla. 2 o vi) una molécula de ácido nucleico que hibrida en condiciones equivalentes a la hibridacion en 7% de dodecilsulfato de sodio (SDS), 0,5 M de NaPO4, 1 mM de EDTA a 50sC con lavado en 2 X SSC, 0,1% de SDS a 50sC a una molécula de ácido nucleico que comprende al menos 50 nucleotidos consecutivos de una secuencia de nucleotidos que aumenta la transcripcion descripta por la SEQ ID Ns: 1 a 15 o el complemento de esta.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US23823309P | 2009-08-31 | 2009-08-31 | |
| EP09169017 | 2009-08-31 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR078009A1 true AR078009A1 (es) | 2011-10-05 |
Family
ID=42752300
Family Applications (5)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP100103187A AR078009A1 (es) | 2009-08-31 | 2010-08-31 | Moleculas de acido nucleico regulatorias para aumentar la expresion genica especifica de semilla y/o preferencial de semilla en las plantas |
| ARP190101862A AR115682A2 (es) | 2009-08-31 | 2019-07-02 | Moléculas de ácido nucleico regulatorias para aumentar la expresión génica específica de semilla y/o preferencial de semilla en las plantas |
| ARP190101860A AR115680A2 (es) | 2009-08-31 | 2019-07-02 | Moléculas de ácido nucleico regulatorias para aumentar la expresión génica específica de semilla y/o preferencial de semilla en las plantas |
| ARP190101861A AR115681A2 (es) | 2009-08-31 | 2019-07-02 | Moléculas de ácido nucleico regulatorias para aumentar la expresión génica específica de semilla y/o preferencial de semilla en las plantas |
| ARP190101859A AR115679A2 (es) | 2009-08-31 | 2019-07-02 | Moléculas de ácido nucleico regulatorias para aumentar la expresión génica específica de semilla y/o preferencial de semilla en las plantas |
Family Applications After (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP190101862A AR115682A2 (es) | 2009-08-31 | 2019-07-02 | Moléculas de ácido nucleico regulatorias para aumentar la expresión génica específica de semilla y/o preferencial de semilla en las plantas |
| ARP190101860A AR115680A2 (es) | 2009-08-31 | 2019-07-02 | Moléculas de ácido nucleico regulatorias para aumentar la expresión génica específica de semilla y/o preferencial de semilla en las plantas |
| ARP190101861A AR115681A2 (es) | 2009-08-31 | 2019-07-02 | Moléculas de ácido nucleico regulatorias para aumentar la expresión génica específica de semilla y/o preferencial de semilla en las plantas |
| ARP190101859A AR115679A2 (es) | 2009-08-31 | 2019-07-02 | Moléculas de ácido nucleico regulatorias para aumentar la expresión génica específica de semilla y/o preferencial de semilla en las plantas |
Country Status (17)
| Country | Link |
|---|---|
| US (5) | US9150871B2 (es) |
| EP (5) | EP3156490B1 (es) |
| JP (1) | JP5794987B2 (es) |
| KR (1) | KR20120046788A (es) |
| CN (3) | CN102575259B (es) |
| AR (5) | AR078009A1 (es) |
| AU (5) | AU2010288760B2 (es) |
| BR (4) | BR122021003073B1 (es) |
| CA (3) | CA3167819A1 (es) |
| CL (1) | CL2012000553A1 (es) |
| DE (1) | DE112010003493T5 (es) |
| IL (1) | IL218095A0 (es) |
| MX (1) | MX2012002069A (es) |
| RU (2) | RU2559534C2 (es) |
| SG (1) | SG178388A1 (es) |
| WO (1) | WO2011023539A1 (es) |
| ZA (1) | ZA201202245B (es) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2421976B1 (en) | 2009-04-22 | 2015-09-16 | BASF Plant Science Company GmbH | Whole seed specific promoter |
| CN102597245A (zh) | 2009-08-31 | 2012-07-18 | 巴斯夫植物科学有限公司 | 用于在植物中增强种子特异性基因表达而促进增强的多不饱和脂肪酸合成的调节性核酸分子 |
| CA3175433C (en) | 2009-08-31 | 2024-05-28 | Basf Plant Science Company Gmbh | Regulatory nucleic acid molecules for enhancing constitutive gene expression in plants |
| CN102575259B (zh) | 2009-08-31 | 2017-04-12 | 巴斯夫植物科学有限公司 | 用于增强植物中种子特异的和/或种子优先的基因表达的调节性核酸分子 |
| MX2013010197A (es) | 2011-03-18 | 2013-09-26 | Basf Plant Science Co Gmbh | Promotores para regular la expresion en plantas. |
| WO2013038294A1 (en) * | 2011-09-15 | 2013-03-21 | Basf Plant Science Company Gmbh | Regulatory nucleic acid molecules for reliable gene expression in plants |
| EP2677035A1 (en) | 2012-06-22 | 2013-12-25 | BASF Plant Science Company GmbH | Plants having enhanced yield-related traits and a method for making the same |
| EP2816115A1 (en) | 2013-06-17 | 2014-12-24 | BASF Plant Science Company GmbH | Plants having one or more enhanced yield-related traits and a method for making the same |
| US10731169B2 (en) | 2013-07-05 | 2020-08-04 | Basf Plant Science Company Gmbh | Gene expression or activity enhancing elements |
| EP2896698A1 (en) | 2014-01-17 | 2015-07-22 | BASF Plant Science Company GmbH | Plants having one or more enhanced yield-related traits and a method for making the same |
| CA3138329A1 (en) * | 2019-05-10 | 2020-11-19 | Basf Se | Regulatory nucleic acid molecules for enhancing gene expression in plants |
| BR112022004545A2 (pt) * | 2019-09-12 | 2022-05-31 | Basf Se | Métodos para aumentar a expressão derivada de um promotor vegetal e produzir uma planta, construção de expressão recombinante, vetor de expressão, célula ou planta transgênica, cultura de células transgênicas e usos |
| WO2021069387A1 (en) * | 2019-10-07 | 2021-04-15 | Basf Se | Regulatory nucleic acid molecules for enhancing gene expression in plants |
| WO2024083579A1 (en) * | 2022-10-20 | 2024-04-25 | Basf Se | Regulatory nucleic acid molecules for enhancing gene expression in plants |
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| WO1993020216A1 (en) * | 1991-02-22 | 1993-10-14 | University Technologies International, Inc. | Oil-body protein cis-elements as regulatory signals |
| US5591616A (en) | 1992-07-07 | 1997-01-07 | Japan Tobacco, Inc. | Method for transforming monocotyledons |
| AU691550B2 (en) | 1993-12-09 | 1998-05-21 | Thomas Jefferson University | Compounds and methods for site-directed mutations in eukaryotic cells |
| US5750866A (en) * | 1994-09-08 | 1998-05-12 | American Cyanamid Company | AHAS promoter useful for expression of introduced genes in plants |
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| EP2221381A3 (en) | 2000-06-23 | 2010-10-27 | Syngenta Participations AG | Promoters for regulation of plant expression |
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| US20100170002A1 (en) * | 2006-03-24 | 2010-07-01 | Ceres, Inc. | Promoter, promoter control elements, and combinations, and uses thereof |
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| RU2415566C2 (ru) * | 2004-07-30 | 2011-04-10 | Басф Агрокемикал Продактс Б.В. | Устойчивые к гербицидам растения подсолнечника, полинуклеотиды, кодирующие устойчивые к гербицидам белки большой субъединицы ацетогидроксиацидсинтазы, и способы применения |
| EP1794305A2 (en) | 2004-09-23 | 2007-06-13 | BASF Plant Science GmbH | Recombination cassettes and methods for sequence excision in plants |
| EP2166098B1 (en) | 2004-10-05 | 2013-11-06 | SunGene GmbH | Constitutive expression cassettes for regulation of plant expression |
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| JP2009529863A (ja) | 2006-03-17 | 2009-08-27 | ビーエーエスエフ プラント サイエンス ゲーエムベーハー | ダイズに対するｄ−アミノ酸選択 |
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| WO2008104559A1 (de) * | 2007-02-27 | 2008-09-04 | Norddeutsche Pflanzenzucht | Verfahren zur herstellung von mehrfach ungesättigten fettsäuren in transgenen organismen |
| ATE528412T1 (de) | 2007-07-31 | 2011-10-15 | Basf Plant Science Gmbh | Desaturasen und verfahren zur herstellung mehrfach ungesättigter fettsäuren in transgenen organismen |
| BRPI0816880A2 (pt) | 2007-09-21 | 2015-09-08 | Basf Plant Science Gmbh | métodos para produzir uma planta com rendimento aumentado em comparação com uma planta do tipo selvagem correspondente, para produzir uma planta transgênica com rendimento aumentado em comparação com uma planta do tipo selvagem não transformada correspondente, para produzir uma composição agrícola, para identificar uma planta com um rendimento aumentado, e para aumentar o rendimento de uma população de plantas, molécula de ácido nucleico isolada, construção de ácido nucléico, vetor, processos para produzir um polipeptídeo, e para identificar um composto, polipeptídeo, anticorpo, núcleo de célula de planta, célula de planta, tecido de planta, material de propagação, pólen, progênie, material colhido ou planta, semente, parte de planta, planta transgênica, planta transgênica, núcleo de célula de planta transgênica, célula de planta transgênica, planta que compreende um ou mais de tais núcleos de célula de planta transgênica ou células de planta, progênie, semente ou pólen derivado de ou produzido por uma planta transgênica, composição, e, uso dos ácidos nucleicos. |
| ES2556358T3 (es) | 2009-03-23 | 2016-01-15 | Satyatek Sa | Sistema y procedimiento para la lectura de una o varias etiquetas RFID en un estuche metálico con un protocolo anticolisión |
| CN102597245A (zh) | 2009-08-31 | 2012-07-18 | 巴斯夫植物科学有限公司 | 用于在植物中增强种子特异性基因表达而促进增强的多不饱和脂肪酸合成的调节性核酸分子 |
| CA3175433C (en) | 2009-08-31 | 2024-05-28 | Basf Plant Science Company Gmbh | Regulatory nucleic acid molecules for enhancing constitutive gene expression in plants |
| CN102575259B (zh) | 2009-08-31 | 2017-04-12 | 巴斯夫植物科学有限公司 | 用于增强植物中种子特异的和/或种子优先的基因表达的调节性核酸分子 |
| WO2013038294A1 (en) * | 2011-09-15 | 2013-03-21 | Basf Plant Science Company Gmbh | Regulatory nucleic acid molecules for reliable gene expression in plants |
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2010
- 2010-08-11 CN CN201080038714.1A patent/CN102575259B/zh active Active
- 2010-08-11 CN CN202010460012.8A patent/CN111560375A/zh active Pending
- 2010-08-11 BR BR122021003073-1A patent/BR122021003073B1/pt active IP Right Grant
- 2010-08-11 CN CN201710157168.7A patent/CN106947762B/zh active Active
- 2010-08-11 SG SG2012009932A patent/SG178388A1/en unknown
- 2010-08-11 EP EP16195551.3A patent/EP3156490B1/en active Active
- 2010-08-11 BR BR122021003079-0A patent/BR122021003079B1/pt active IP Right Grant
- 2010-08-11 RU RU2012112346/10A patent/RU2559534C2/ru active
- 2010-08-11 CA CA3167819A patent/CA3167819A1/en active Pending
- 2010-08-11 EP EP16195553.9A patent/EP3156491B1/en active Active
- 2010-08-11 KR KR1020127008217A patent/KR20120046788A/ko not_active Application Discontinuation
- 2010-08-11 JP JP2012525979A patent/JP5794987B2/ja not_active Expired - Fee Related
- 2010-08-11 BR BR122021003080-4A patent/BR122021003080B1/pt active IP Right Grant
- 2010-08-11 CA CA2771253A patent/CA2771253C/en active Active
- 2010-08-11 RU RU2015125627A patent/RU2692924C2/ru active
- 2010-08-11 EP EP10740678.7A patent/EP2473608B1/en active Active
- 2010-08-11 CA CA3048833A patent/CA3048833C/en active Active
- 2010-08-11 EP EP16194406.1A patent/EP3153584B1/en active Active
- 2010-08-11 US US13/393,045 patent/US9150871B2/en not_active Expired - Fee Related
- 2010-08-11 EP EP16195556.2A patent/EP3156492B1/en active Active
- 2010-08-11 DE DE112010003493T patent/DE112010003493T5/de not_active Withdrawn
- 2010-08-11 AU AU2010288760A patent/AU2010288760B2/en not_active Ceased
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