AR070929A1 - Metodo para la identificacion de adn genomico en una muestra - Google Patents

Metodo para la identificacion de adn genomico en una muestra

Info

Publication number
AR070929A1
AR070929A1 ARP090100962A ARP090100962A AR070929A1 AR 070929 A1 AR070929 A1 AR 070929A1 AR P090100962 A ARP090100962 A AR P090100962A AR P090100962 A ARP090100962 A AR P090100962A AR 070929 A1 AR070929 A1 AR 070929A1
Authority
AR
Argentina
Prior art keywords
fragments
dna
organism
genomic
genomic dna
Prior art date
Application number
ARP090100962A
Other languages
English (en)
Original Assignee
Expressive Res Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Expressive Res Bv filed Critical Expressive Res Bv
Publication of AR070929A1 publication Critical patent/AR070929A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Método para analizar una region genomica de un organismo, que comprende cuatro partes principales. La primera parte involucra el aislamiento de ARNm de un organismo seleccionado que se utiliza para la preparacion de pequenos fragmentos de ADN de unica hebra con un adaptador que contiene una etiqueta de afinidad. Estos fragmentos de ADN se utilizan en la parte tres. En la segunda parte, se aísla ADN genomico del mismo organismo o de un organismo relacionado. Este ADN genomico es fragmentado y ligado a moléculas adaptadoras. En la tercera parte, estos fragmentos genomicos son hibridados con fragmentos de ADN de unica hebra de la parte uno y los híbridos formados en este proceso son utilizados para la síntesis de fragmentos de ADN. Estos fragmentos serán utilizados en la parte cuatro, la cual involucra la secuenciacion de estos fragmentos utilizando uno de los métodos de secuenciacion de alto rendimiento disponibles. El método descripto hace posible determinar secuencias en una parte seleccionada del ADN genomico que representa las regiones codificantes de la mayoría de genes expresados. La comparacion de dichas partes seleccionadas entre diferentes individuos permite la identificacion de sitios polimorficos. Esto puede dar como resultado el descubrimiento de marcadores por cada característica, siendo la obtencion de marcadores util en tecnologías de mejoramiento genético.
ARP090100962A 2008-03-17 2009-03-17 Metodo para la identificacion de adn genomico en una muestra AR070929A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP08152859 2008-03-17

Publications (1)

Publication Number Publication Date
AR070929A1 true AR070929A1 (es) 2010-05-12

Family

ID=39472820

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP090100962A AR070929A1 (es) 2008-03-17 2009-03-17 Metodo para la identificacion de adn genomico en una muestra

Country Status (15)

Country Link
US (1) US9695469B2 (es)
EP (1) EP2268834B1 (es)
CN (1) CN102027136A (es)
AR (1) AR070929A1 (es)
AU (1) AU2009226248B8 (es)
BR (1) BRPI0908734A2 (es)
CA (1) CA2718905A1 (es)
DK (1) DK2268834T3 (es)
ES (1) ES2528971T3 (es)
IL (1) IL208237A0 (es)
NZ (1) NZ588112A (es)
RU (1) RU2010142289A (es)
TW (1) TW200948969A (es)
WO (1) WO2009116863A2 (es)
ZA (1) ZA201006797B (es)

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PL2756098T3 (pl) 2011-09-16 2018-12-31 Lexogen Gmbh Sposób wytwarzania biblioteki cząsteczek kwasów nukleinowych
EP2929056A4 (en) * 2012-12-10 2016-11-09 Resolution Bioscience Inc METHODS FOR TARGETED GENOMIC ANALYSIS
CN105400864B (zh) * 2014-09-12 2020-04-14 深圳华大基因股份有限公司 用于基于血液样品构建测序文库的方法及其在确定胎儿遗传异常中的用途
JP6483249B2 (ja) * 2014-09-12 2019-03-13 エムジーアイ テック カンパニー リミテッドＭＧＩ Ｔｅｃｈ Ｃｏ．， Ｌｔｄ． 単離されたオリゴヌクレオチドおよび核酸の配列決定におけるその使用
CN106319639B (zh) * 2015-06-17 2018-09-04 深圳华大智造科技有限公司 构建测序文库的方法及设备
ITUA20162640A1 (it) * 2016-04-15 2017-10-15 Menarini Silicon Biosystems Spa Metodo e kit per la generazione di librerie di dna per sequenziamento massivo parallelo
WO2018057928A1 (en) * 2016-09-23 2018-03-29 Grail, Inc. Methods of preparing and analyzing cell-free nucleic acid sequencing libraries
AU2019280712A1 (en) 2018-06-06 2021-01-07 The Regents Of The University Of California Methods of producing nucleic acid libraries and compositions and kits for practicing same
CN110093406A (zh) * 2019-05-27 2019-08-06 新疆农业大学 一种盘羊及其杂交后代遗传基因研究方法
CA3194398A1 (en) * 2020-10-05 2022-04-14 Twist Bioscience Corporation Hybridization methods and reagents
EP4279590A1 (en) * 2022-05-19 2023-11-22 Miltenyi Biotec B.V. & Co. KG Method for generation of a nucleic acid library

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Also Published As

Publication number Publication date
EP2268834B1 (en) 2015-01-07
AU2009226248B8 (en) 2014-10-02
US9695469B2 (en) 2017-07-04
WO2009116863A2 (en) 2009-09-24
DK2268834T3 (en) 2015-02-02
AU2009226248A2 (en) 2013-02-14
BRPI0908734A2 (pt) 2015-07-28
AU2009226248A1 (en) 2009-09-24
RU2010142289A (ru) 2012-04-27
EP2268834A2 (en) 2011-01-05
IL208237A0 (en) 2010-12-30
US20110105338A1 (en) 2011-05-05
AU2009226248B2 (en) 2014-09-18
CA2718905A1 (en) 2009-09-24
WO2009116863A3 (en) 2009-11-12
ZA201006797B (en) 2011-10-26
NZ588112A (en) 2013-04-26
TW200948969A (en) 2009-12-01
CN102027136A (zh) 2011-04-20
AU2009226248A8 (en) 2014-10-02
ES2528971T3 (es) 2015-02-13

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