AR048074A1 - Proteinas de fusion de complejos de toxinas insecticidas - Google Patents

Proteinas de fusion de complejos de toxinas insecticidas

Info

Publication number
AR048074A1
AR048074A1 ARP050100794A ARP050100794A AR048074A1 AR 048074 A1 AR048074 A1 AR 048074A1 AR P050100794 A ARP050100794 A AR P050100794A AR P050100794 A ARP050100794 A AR P050100794A AR 048074 A1 AR048074 A1 AR 048074A1
Authority
AR
Argentina
Prior art keywords
seq
class
protein
fusion proteins
polypeptide
Prior art date
Application number
ARP050100794A
Other languages
English (en)
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of AR048074A1 publication Critical patent/AR048074A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Catching Or Destruction (AREA)

Abstract

Proteínas de fusion del Complejo de Toxinas (ôTCö) insecticidas y polinucleotidos, que codifican estas proteínas de fusion. Además, polinucleotidos que codifican dichas proteínas de fusion de TC, y vectores que comprenden dichos polinucleotidos. Entre otras, se provee una proteína de fusion que comprende una proteína de Clase A, una proteína de Clase B, y una proteína de Clase C de TC fusionadas conjuntamente para formar una unica proteína. Además, se provee una proteína de fusion que comprende una proteína de TC de Clase B, y una de Clase C fusionadas conjuntamente. La proteína de fusion BC o CB puede usarse para intensificar o potenciar la actividad anti-insecto de una ôToxina Aö o proteína de Clase A. Hasta hora, no había expectativas de que dichas proteínas de fusion funcionaran apropiadamente y mantuvieran su actividad cuando se fusionaran conjuntamente. De este modo se reduce de manera ventajosa el numero de genes necesarios para transformar plantas. Para tal fin se incluye, además, plantas, células (células bacterianas y de plantas, por ejemplo), y semillas que comprenden dichos polinucleotidos. Dichas plantas pueden producir las proteínas de fusion que les confieren resistencia a los insectos se incluye, por ultimo, métodos para controlar plagas (con preferencia insectos y otras plagas de las plantas) con dichas proteínas de fusion. Reivindicacion 1: Una proteína de fusion aislada que comprende un polipéptido del Complejo de Toxinas Clase B y un polipéptido del Complejo de Toxinas Clase C en la cual: dicho polipéptido de Clase B es un potenciador de 130-180 kDa que tiene una secuencia de aminoácidos por lo menos 40 % idéntica a una secuencia seleccionada del grupo formado por: TcdB1 (SEC ID NO: 5); TcdB2 (SEC ID NO: 6); TcaC (SEC ID NO: 7); XptC1wi (SEC ID NO: 8); XptB1xb (SEC ID NO: 9); PptB11529 (SEC ID NO: 10); y Sep B (SEC ID NO: 11); y dicho polipéptido de Clase C es un potenciador de 90-112 kDa que tiene una secuencia de aminoácidos por lo menos 35 % idéntica a una secuencia seleccionada del grupo formado por: TccC1 (SEC ID NO: 12); TccC2 (SEC ID NO: 13); TccC3 (SEC ID NO: 14); TccC (SEC ID NO: 15); TccC5 (SEC ID NO: 16); XptB1wi (SEC ID NO: 17); XptC1xb (SEC ID NO: 18); PptC1 (larga) (SEC ID NO: 19); PptC1 (corta) (SEC ID NO: 20); y SepC (SEC ID NO: 21) y dichos polipéptidos de Clase B y de Clase C son capaces de intensificar la actividad insecticida de un polipéptido del Complejo de Toxinas de Clase A de 230- 290 kD.
ARP050100794A 2004-03-02 2005-03-02 Proteinas de fusion de complejos de toxinas insecticidas AR048074A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US54951604P 2004-03-02 2004-03-02
US54950204P 2004-03-02 2004-03-02

Publications (1)

Publication Number Publication Date
AR048074A1 true AR048074A1 (es) 2006-03-29

Family

ID=34922715

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP050100794A AR048074A1 (es) 2004-03-02 2005-03-02 Proteinas de fusion de complejos de toxinas insecticidas

Country Status (6)

Country Link
US (2) US7605228B2 (es)
EP (1) EP1756148A4 (es)
AR (1) AR048074A1 (es)
AU (1) AU2005218598A1 (es)
CA (1) CA2558656A1 (es)
WO (1) WO2005084355A2 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005067491A2 (en) 2004-01-07 2005-07-28 Dow Agrosciences Llc Toxin complex proteins and genes from xenorhabdus bovienii
US7812131B2 (en) 2005-03-02 2010-10-12 Doe AgroSciences LLC Sources for, and types of, insecticidally active proteins, and polynucleotides that encode the proteins
US9688730B2 (en) 2012-07-02 2017-06-27 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP2986725A4 (en) * 2013-04-19 2016-11-09 Agres Ltd METHOD AND MATERIALS FOR THE SECULATION OF PROTEINS
TW201538518A (zh) * 2014-02-28 2015-10-16 Dow Agrosciences Llc 藉由嵌合基因調控元件所賦予之根部特異性表現
US10968257B2 (en) * 2018-04-03 2021-04-06 The Broad Institute, Inc. Target recognition motifs and uses thereof
US11891613B2 (en) 2020-12-17 2024-02-06 Pioneer Hi-Bred International, Inc. Bacterial strains with toxin complex for insect control

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6528484B1 (en) 1993-05-18 2003-03-04 Wisconsin Alumni Research Foundation Insecticidal protein toxins from Photorhabdus
AUPO808897A0 (en) 1997-07-17 1997-08-14 Commonwealth Scientific And Industrial Research Organisation Toxin genes from the bacteria xenorhabdus nematophilus and photohabdus luminescens
US6281413B1 (en) * 1998-02-20 2001-08-28 Syngenta Participations Ag Insecticidal toxins from Photorhabdus luminescens and nucleic acid sequences coding therefor
US6639129B2 (en) * 2000-03-24 2003-10-28 Wisconsin Alumni Research Foundation DNA sequences from photorhabdus luminescens
EP1379549A2 (fr) * 2001-02-07 2004-01-14 Institut Pasteur Sequence du genome de photorhabdus luminescens souche tto1 et utilisations
ES2311731T3 (es) * 2002-06-28 2009-02-16 Dow Agrosciences Llc Proteinas de accion pesticida y polinucleotidos que se pueden obtener a partir de especies de paenibacillus.
EP1563072A2 (en) * 2002-11-12 2005-08-17 University Of Bath Dna sequences from tcd genomic region of photorhabdus luminescens
WO2004067750A2 (en) 2003-01-21 2004-08-12 Dow Agrosciences Llc Xenorhabdus tc proteins and genes for pest control
EP1585819A2 (en) * 2003-01-21 2005-10-19 Dow Agrosciences LLC Mixing and matching tc proteins for pest control
JP4498233B2 (ja) * 2005-06-30 2010-07-07 キヤノン株式会社 画像処理装置および画像処理方法

Also Published As

Publication number Publication date
EP1756148A2 (en) 2007-02-28
US7605228B2 (en) 2009-10-20
AU2005218598A1 (en) 2005-09-15
US8106159B2 (en) 2012-01-31
EP1756148A4 (en) 2007-09-26
US20100041610A1 (en) 2010-02-18
WO2005084355A2 (en) 2005-09-15
US20060168683A1 (en) 2006-07-27
CA2558656A1 (en) 2005-09-15
WO2005084355A3 (en) 2006-08-31

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