AR042960A1 - Antigenos recombinantes del virus de la hepatitis a obtenidos en celulas vegetales - Google Patents

Antigenos recombinantes del virus de la hepatitis a obtenidos en celulas vegetales

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Publication number
AR042960A1
AR042960A1 ARP040100261A ARP040100261A AR042960A1 AR 042960 A1 AR042960 A1 AR 042960A1 AR P040100261 A ARP040100261 A AR P040100261A AR P040100261 A ARP040100261 A AR P040100261A AR 042960 A1 AR042960 A1 AR 042960A1
Authority
AR
Argentina
Prior art keywords
seq
nucleotide sequence
proteins
sequence
virus antigens
Prior art date
Application number
ARP040100261A
Other languages
English (en)
Inventor
Quezada Alina Lopez
Badillo Beatriz Gonzalez
Sosa Guillermo Selman-Housein
Velasquez Abel Hernandez
Bacallao Javier Rios
Ayon Yamilka Rosabal
Martinez Marlen Perez
Lay Licel Rodriguez
Gonzalez Rolando Garcia
Original Assignee
Ct Ingenieria Genetica Biotech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Ct Ingenieria Genetica Biotech filed Critical Ct Ingenieria Genetica Biotech
Publication of AR042960A1 publication Critical patent/AR042960A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/32011—Picornaviridae
    • C12N2770/32411—Hepatovirus, i.e. hepatitis A virus
    • C12N2770/32422—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Communicable Diseases (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Oncology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

Generación de construcciones genéticas en fragmentos modificados del genoma del virus de la hepatitis A (VHA), utilizando la cepa M2, aislada en Cuba. Las secuencias nucleotídicas de estos fragmentos fusionados a senales de localización y de regulación apropiadas se expresan en plantas transgénicas, dando lugar a antígenos recombinantes del VHA, compuestos por pentámeros y/o envolturas vacías, capaces de generar una respuesta inmune. Reivindicación 1: Antígenos recombinantes del virus de la Hepatitis A según la reivindicación 1 y 2 caracterizado porque se obtienen a partir de la expresión de un gen quimérico según la SEQ ID NO 17 que contiene la fusión de los elementos siguientes: a) un secuencia nucleotídica que codifica para las proteínas VP2, VP3, VP1 y 2A SEQ ID NO 25; b) una secuencia nucleotídica que codifica para las proteínas 3A, 3B, 3C, Seq Id No. 13. Reivindicación 7: antígenos recombinantes del virus de la Hepatitis A según la reivindicación 6 caracterizado porque se expresa en tabaco, zanahoria y en frutos de plantas comestibles. Reivindicación 9: Antígenos recombinantes del virus de la Hepatitis A según la reivindicación 8 caracterizado porque se expresa en arroz y en frutos de plantas comestibles. Reivindicación 11: Antígenos recombinantes del virus de la Hepatitis A según la reivindicación 10 caracterizado porque se obtuvo a partir de la expresión de un gen quimérico que contiene la fusión de los dos elementos siguientes: a) un secuencia nucleotídica según la SEQ ID NO 6 que codifica para las proteínas VP4, VP2, VP3, y 2A; b) una secuencia nucleotídica que codifica para las proteínas 3A, 3B, 3C, según la reivindicación 3b. Reivindicación 18: Antígenos recombinantes del virus de la Hepatitis A según la reivindicación 2 caracterizado porque se obtienen a partir de la expresión coordinada de dos genes quiméricos: a) una secuencia nucleotídica, según la SEQ ID NO 24 que codifica para las proteínas VP2, VP3, VP1, 2A, fusionada en su extremo 5' a una secuencia senal y por su extremo 3' a una secuencia espaciadora seguido de la secuencia que codifica para el péptido KDEL; b) una secuencia nucleotídica, según la SEQ ID NO 23 que codifica para las proteínas 3A, 3B, 3C, referidas en la reivindicación 3b, fusionadas en su extremo 5' a una secuencia senal y por su extremo 3' a una secuencia espaciadora seguido de la secuencia que codifica para el péptido KDEL. Reivindicación 25: Antígenos recombinantes del virus de la Hepatitis A según la reivindicación 10 caracterizado porque se obtienen a partir de la expresión coordinada de dos genes quiméricos: a) una secuencia nucleotídica, según la SEQ ID NO 22 que codifica para las proteínas VP4, VP2, VP3, VP1, 2A, fusionada en su extremo 5' a una secuencia senal y por su extremo 3' a una secuencia espaciadora y seguido de la secuencia que codifica para el péptido KDEL.
ARP040100261A 2003-01-31 2004-01-29 Antigenos recombinantes del virus de la hepatitis a obtenidos en celulas vegetales AR042960A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU20030031A CU23202A1 (es) 2003-01-31 2003-01-31 ANTIGENOS RECOMBINANTES DEL VIRUS DE LA HEPATITIS A OBTENIDOS EN CéLULAS VEGETALES

Publications (1)

Publication Number Publication Date
AR042960A1 true AR042960A1 (es) 2005-07-13

Family

ID=40091669

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP040100261A AR042960A1 (es) 2003-01-31 2004-01-29 Antigenos recombinantes del virus de la hepatitis a obtenidos en celulas vegetales

Country Status (14)

Country Link
US (1) US20060147469A1 (es)
EP (1) EP1589111A1 (es)
JP (1) JP2006514078A (es)
KR (1) KR20050100365A (es)
CN (1) CN1745174A (es)
AR (1) AR042960A1 (es)
AU (1) AU2003291913A1 (es)
BR (1) BR0318026A (es)
CA (1) CA2510482A1 (es)
CU (1) CU23202A1 (es)
MX (1) MXPA05008221A (es)
RU (1) RU2005127340A (es)
WO (1) WO2004067747A1 (es)
ZA (1) ZA200505187B (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2615372A1 (en) * 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
KR101012830B1 (ko) * 2008-04-25 2011-02-10 메디칸(주) 신규 ａ형 간염 바이러스의 항원 유전자 및 상기 유전자로형질전환된 식물체

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5516630A (en) * 1983-09-30 1996-05-14 The United States Of America As Represented By The Department Of Health And Human Services Methods of detecting hepatitis A virus
DE3484244D1 (de) * 1983-09-30 1991-04-11 Massachusetts Inst Technology Herstellung von cdna die hepatitis a virale sequenzen darstellen.
US4614793A (en) * 1983-10-14 1986-09-30 Merck & Co., Inc. Hepatitis A--subunit antigen
SU1469856A1 (ru) * 1986-07-31 1990-09-30 Институт биоорганической химии им.М.М.Шемякина Способ конструировани рекомбинантной плазмидной ДНК, кодирующей синтез полипептидных субстанций против гепатита А
US5294548A (en) * 1990-04-02 1994-03-15 American Biogenetic Sciences, Inc Recombianant Hepatitis a virus
US5612487A (en) * 1991-08-26 1997-03-18 Edible Vaccines, Inc. Anti-viral vaccines expressed in plants
AU776855B2 (en) * 1998-12-23 2004-09-23 Boyce Thompson Institute For Plant Research Inc. Expression of immunogenic hepatitis B surface antigens in transgenic plants
US6368602B1 (en) * 2000-06-16 2002-04-09 Hadasit Medical Research Services And Development Ltd Mucosal immunization against hepatitis A virus (HAV) through rectal administration of HAV vaccine

Also Published As

Publication number Publication date
CU23202A1 (es) 2007-05-18
BR0318026A (pt) 2005-12-06
AU2003291913A1 (en) 2004-08-23
RU2005127340A (ru) 2006-03-10
WO2004067747A1 (es) 2004-08-12
CA2510482A1 (en) 2004-08-12
JP2006514078A (ja) 2006-04-27
US20060147469A1 (en) 2006-07-06
CN1745174A (zh) 2006-03-08
ZA200505187B (en) 2006-04-26
MXPA05008221A (es) 2005-10-05
KR20050100365A (ko) 2005-10-18
EP1589111A1 (en) 2005-10-26

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