AR039938A1 - MODIFIED PHYTASES - Google Patents
MODIFIED PHYTASESInfo
- Publication number
- AR039938A1 AR039938A1 ARP030101936A ARP030101936A AR039938A1 AR 039938 A1 AR039938 A1 AR 039938A1 AR P030101936 A ARP030101936 A AR P030101936A AR P030101936 A ARP030101936 A AR P030101936A AR 039938 A1 AR039938 A1 AR 039938A1
- Authority
- AR
- Argentina
- Prior art keywords
- phytase
- modified
- phytases
- model
- compared
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fodder In General (AREA)
Abstract
La presente describe fitasas modificadas. Estas fitasas están modificadas en comparación con una fitasa modelo en diversas posiciones, en particular para incrementar la termoestabilidad de la fitasa modificada en comparación con la de la fitasa modelo. Las fitasas modificadas han retenido otras propiedades favorables de la fitasa de Aspergillus niger, en particular porque en la fitasa modificada se han retenido residuos de aminoácidos específicos de la fitasa de Aspergillus niger. Polinucleótidos que codifican para dichas fitasas, vector, célula huésped, procedimientos para la producción de los polipéptidos y composición que los comprende. Reivindicación 1: Un polipéptido, caracterizado porque cuando se alinea con una fitasa modelo está ,modificado en comparación con dicha fitasa modelo en al menos una de las siguientes posiciones: 5, 6, 13, 19, 21, 29, 31, 36, 39, 43, 53, 69, 78, 81, 85, 87, 99, 112, 113, 122, 125, 126, 128,137,147, 148, 157,160, 163, 165, 169, 172, 176, 178, 180, 181, 182, 183, 189, 194, 197, 201, 203, 211, 213, 215, 218, 222, 223, 225, 232, 233, 242, 246, 247, 248, 249, 250, 251, 252, 254, 269, 291, 296, 310, 312, 315, 322, 330, 342, 346, 362, 365, 367, 368, 372, 374, 375, 382, 384, 395, 414, 417, 425, 428, 438, 440.This describes modified phytases. These phytases are modified compared to a phytase model in various positions, in particular to increase the thermostability of the modified phytase compared to that of the phytase model. Modified phytases have retained other favorable properties of Aspergillus niger phytase, in particular because specific amino acid residues of Aspergillus niger phytase have been retained in the modified phytase. Polynucleotides encoding said phytases, vector, host cell, procedures for the production of the polypeptides and composition comprising them. Claim 1: A polypeptide, characterized in that when aligned with a model phytase, it is modified compared to said model phytase in at least one of the following positions: 5, 6, 13, 19, 21, 29, 31, 36, 39 , 43, 53, 69, 78, 81, 85, 87, 99, 112, 113, 122, 125, 126, 128,137,147, 148, 157,160, 163, 165, 169, 172, 176, 178, 180, 181, 182 , 183, 189, 194, 197, 201, 203, 211, 213, 215, 218, 222, 223, 225, 232, 233, 242, 246, 247, 248, 249, 250, 251, 252, 254, 269 , 291, 296, 310, 312, 315, 322, 330, 342, 346, 362, 365, 367, 368, 372, 374, 375, 382, 384, 395, 414, 417, 425, 428, 438, 440 .
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP02100608 | 2002-05-30 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR039938A1 true AR039938A1 (en) | 2005-03-09 |
Family
ID=29595046
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP030101936A AR039938A1 (en) | 2002-05-30 | 2003-05-30 | MODIFIED PHYTASES |
| ARP030101937A AR039939A1 (en) | 2002-05-30 | 2003-05-30 | MODIFIED PHYTASES |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP030101937A AR039939A1 (en) | 2002-05-30 | 2003-05-30 | MODIFIED PHYTASES |
Country Status (16)
| Country | Link |
|---|---|
| EP (1) | EP1513931A2 (en) |
| JP (1) | JP2005533492A (en) |
| KR (1) | KR20050038593A (en) |
| CN (1) | CN1656217A (en) |
| AR (2) | AR039938A1 (en) |
| AU (1) | AU2003238448A1 (en) |
| BR (1) | BR0311287A (en) |
| CA (1) | CA2485513A1 (en) |
| MX (1) | MXPA04011224A (en) |
| NO (1) | NO20044802L (en) |
| PE (1) | PE20040537A1 (en) |
| PL (1) | PL372824A1 (en) |
| RU (1) | RU2329301C2 (en) |
| TW (2) | TW200401033A (en) |
| WO (1) | WO2003102174A2 (en) |
| ZA (1) | ZA200410369B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0422052D0 (en) | 2004-10-04 | 2004-11-03 | Dansico As | Enzymes |
| ES2387203T3 (en) | 2006-04-04 | 2012-09-17 | Novozymes A/S | Phytase variants |
| DE102006053059A1 (en) | 2006-11-10 | 2008-05-15 | Ab Enzymes Gmbh | Polypeptide with phytase activity and increased temperature stability of the enzyme activity and this coding nucleotide sequence |
| CN102854158B (en) * | 2011-07-01 | 2016-01-20 | 北京昕大洋科技发展有限公司 | A kind of method of Fast Measurement phytase heat resistance |
| CN105441406A (en) * | 2014-08-05 | 2016-03-30 | 北京大学 | Phytase variant |
| CN108251439B (en) * | 2018-01-11 | 2021-03-30 | 山西大学 | Artificially-modified trypsin-resistant phytase as well as preparation method and application thereof |
| CN117683745A (en) * | 2020-04-29 | 2024-03-12 | 南京百斯杰生物工程有限公司 | Parent phytase variant |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE338110T1 (en) * | 1999-01-22 | 2006-09-15 | Novozymes As | IMPROVED PHYTASES |
-
2003
- 2003-05-28 BR BR0311287-0A patent/BR0311287A/en not_active IP Right Cessation
- 2003-05-28 CA CA002485513A patent/CA2485513A1/en not_active Abandoned
- 2003-05-28 JP JP2004510416A patent/JP2005533492A/en not_active Withdrawn
- 2003-05-28 RU RU2004139054/13A patent/RU2329301C2/en not_active IP Right Cessation
- 2003-05-28 PL PL03372824A patent/PL372824A1/en not_active Application Discontinuation
- 2003-05-28 KR KR1020047019401A patent/KR20050038593A/en not_active Application Discontinuation
- 2003-05-28 CN CNA03812470XA patent/CN1656217A/en active Pending
- 2003-05-28 EP EP03732516A patent/EP1513931A2/en not_active Withdrawn
- 2003-05-28 AU AU2003238448A patent/AU2003238448A1/en not_active Abandoned
- 2003-05-28 WO PCT/EP2003/005729 patent/WO2003102174A2/en active Application Filing
- 2003-05-28 MX MXPA04011224A patent/MXPA04011224A/en not_active Application Discontinuation
- 2003-05-30 AR ARP030101936A patent/AR039938A1/en not_active Application Discontinuation
- 2003-05-30 PE PE2003000534A patent/PE20040537A1/en not_active Application Discontinuation
- 2003-05-30 TW TW092114745A patent/TW200401033A/en unknown
- 2003-05-30 AR ARP030101937A patent/AR039939A1/en not_active Application Discontinuation
- 2003-05-30 TW TW092114788A patent/TW200403341A/en unknown
-
2004
- 2004-11-04 NO NO20044802A patent/NO20044802L/en not_active Application Discontinuation
- 2004-12-23 ZA ZA200410369A patent/ZA200410369B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| BR0311287A (en) | 2005-03-29 |
| TW200403341A (en) | 2004-03-01 |
| WO2003102174A3 (en) | 2004-03-25 |
| ZA200410369B (en) | 2006-06-28 |
| TW200401033A (en) | 2004-01-16 |
| PL372824A1 (en) | 2005-08-08 |
| WO2003102174A2 (en) | 2003-12-11 |
| RU2329301C2 (en) | 2008-07-20 |
| EP1513931A2 (en) | 2005-03-16 |
| AR039939A1 (en) | 2005-03-09 |
| CA2485513A1 (en) | 2003-12-11 |
| AU2003238448A1 (en) | 2003-12-19 |
| NO20044802L (en) | 2004-12-08 |
| PE20040537A1 (en) | 2004-11-04 |
| CN1656217A (en) | 2005-08-17 |
| MXPA04011224A (en) | 2005-01-25 |
| RU2004139054A (en) | 2005-06-27 |
| KR20050038593A (en) | 2005-04-27 |
| JP2005533492A (en) | 2005-11-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA | Abandonment or withdrawal | ||
| FB | Suspension of granting procedure |