RU2004139054A - MODIFIED PHITASES - Google Patents

MODIFIED PHITASES Download PDF

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Publication number
RU2004139054A
RU2004139054A RU2004139054/13A RU2004139054A RU2004139054A RU 2004139054 A RU2004139054 A RU 2004139054A RU 2004139054/13 A RU2004139054/13 A RU 2004139054/13A RU 2004139054 A RU2004139054 A RU 2004139054A RU 2004139054 A RU2004139054 A RU 2004139054A
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RU
Russia
Prior art keywords
polypeptide
seq
polynucleotide
vector
sequence
Prior art date
Application number
RU2004139054/13A
Other languages
Russian (ru)
Other versions
RU2329301C2 (en
Inventor
ВАН ДЕР Ян Метске ЛААН (NL)
Ван Дер Ян Метске Лаан
Штефанус Корнелис Хендрикус Йозеф ТУРК (NL)
Штефанус Корнелис Хендрикус Йозеф ТУРК
Original Assignee
Басф Акциенгезелльшафт (De)
Басф Акциенгезелльшафт
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Publication date
Application filed by Басф Акциенгезелльшафт (De), Басф Акциенгезелльшафт filed Critical Басф Акциенгезелльшафт (De)
Publication of RU2004139054A publication Critical patent/RU2004139054A/en
Application granted granted Critical
Publication of RU2329301C2 publication Critical patent/RU2329301C2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fodder In General (AREA)

Claims (11)

1. Полипептид, имеющий последовательность SEQ ID NO:1, SEQ ID NO:5 или SEQ ID NO:7.1. A polypeptide having the sequence of SEQ ID NO: 1, SEQ ID NO: 5 or SEQ ID NO: 7. 2. Полипептид, имеющий, по меньшей мере, 91, предпочтительно, по меньшей мере, 92, более предпочтительно, по меньшей мере, 93, более предпочтительно, по меньшей мере, 94, более предпочтительно, по меньшей мере, 95, более предпочтительно, по меньшей мере, 96, более предпочтительно, по меньшей мере, 97, более предпочтительно, по меньшей мере, 98, наиболее предпочтительно, по меньшей мере, 99% гомологии (идентичности) с полипептидом по п.1.2. A polypeptide having at least 91, preferably at least 92, more preferably at least 93, more preferably at least 94, more preferably at least 95, more preferably at least 96, more preferably at least 97, more preferably at least 98, most preferably at least 99% homology (identity) with the polypeptide according to claim 1. 3. Полинуклеотид, включающий полинуклеотидную последовательность, кодирующую полипептид по одному из пп.1 и 2.3. A polynucleotide comprising a polynucleotide sequence encoding a polypeptide according to one of claims 1 and 2. 4. Полинукпеотид по п.3, который представляет собой ДНК.4. The polynucleotide according to claim 3, which is DNA. 5. Полинуклеотид по п.3, включающий последовательность SEQ ID NO:2, SEQ ID NO:4 или SEQ ID NO:6.5. The polynucleotide according to claim 3, comprising the sequence of SEQ ID NO: 2, SEQ ID NO: 4 or SEQ ID NO: 6. 6. Вектор, включающий полинуклеотидную последовательность по одному из пп.3-5.6. A vector comprising a polynucleotide sequence according to one of claims 3 to 5. 7. Вектор по п.6, который представляет собой экспрессионный вектор, в котором полинуклеотидная последовательность, кодирующая полипептид, является оперативно связанной с регуляторной последовательностью.7. The vector according to claim 6, which is an expression vector in which the polynucleotide sequence encoding the polypeptide is operably linked to a regulatory sequence. 8. Клетка-хозяин, которая экспримирует как гетерологический белок полипептид по п.1 или 2.8. A host cell that expresses as a heterologous protein a polypeptide according to claim 1 or 2. 9. Клетка-хозяин, трансформированная полинуклеотидом по одному из пп.3-5 или вектором по п.6 или 7.9. A host cell transformed with a polynucleotide according to one of claims 3 to 5 or a vector according to claim 6 or 7. 10. Способ получения полипептида по одному из пп.1 и 2, который включает культивирование клетки-хозяина по п.8 или 9 в условиях, которые обеспечивают экспрессию полипептида.10. The method of producing the polypeptide according to one of claims 1 and 2, which includes culturing the host cell according to claim 8 or 9 under conditions that ensure expression of the polypeptide. 11. Композиция, включающая полипептид по одному из пп.1 и 2.11. A composition comprising a polypeptide according to one of claims 1 and 2.
RU2004139054/13A 2002-05-30 2003-05-28 Modified phytases RU2329301C2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP02100608.5 2002-05-30
EP02100608 2002-05-30

Publications (2)

Publication Number Publication Date
RU2004139054A true RU2004139054A (en) 2005-06-27
RU2329301C2 RU2329301C2 (en) 2008-07-20

Family

ID=29595046

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2004139054/13A RU2329301C2 (en) 2002-05-30 2003-05-28 Modified phytases

Country Status (16)

Country Link
EP (1) EP1513931A2 (en)
JP (1) JP2005533492A (en)
KR (1) KR20050038593A (en)
CN (1) CN1656217A (en)
AR (2) AR039938A1 (en)
AU (1) AU2003238448A1 (en)
BR (1) BR0311287A (en)
CA (1) CA2485513A1 (en)
MX (1) MXPA04011224A (en)
NO (1) NO20044802L (en)
PE (1) PE20040537A1 (en)
PL (1) PL372824A1 (en)
RU (1) RU2329301C2 (en)
TW (2) TW200401033A (en)
WO (1) WO2003102174A2 (en)
ZA (1) ZA200410369B (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0422052D0 (en) 2004-10-04 2004-11-03 Dansico As Enzymes
ES2387203T3 (en) 2006-04-04 2012-09-17 Novozymes A/S Phytase variants
DE102006053059A1 (en) 2006-11-10 2008-05-15 Ab Enzymes Gmbh Polypeptide with phytase activity and increased temperature stability of the enzyme activity and this coding nucleotide sequence
CN102854158B (en) * 2011-07-01 2016-01-20 北京昕大洋科技发展有限公司 A kind of method of Fast Measurement phytase heat resistance
CN105441406A (en) * 2014-08-05 2016-03-30 北京大学 Phytase variant
CN108251439B (en) * 2018-01-11 2021-03-30 山西大学 Artificially-modified trypsin-resistant phytase as well as preparation method and application thereof
CN117683745A (en) * 2020-04-29 2024-03-12 南京百斯杰生物工程有限公司 Parent phytase variant

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE338110T1 (en) * 1999-01-22 2006-09-15 Novozymes As IMPROVED PHYTASES

Also Published As

Publication number Publication date
BR0311287A (en) 2005-03-29
TW200403341A (en) 2004-03-01
WO2003102174A3 (en) 2004-03-25
ZA200410369B (en) 2006-06-28
TW200401033A (en) 2004-01-16
PL372824A1 (en) 2005-08-08
WO2003102174A2 (en) 2003-12-11
RU2329301C2 (en) 2008-07-20
EP1513931A2 (en) 2005-03-16
AR039939A1 (en) 2005-03-09
CA2485513A1 (en) 2003-12-11
AU2003238448A1 (en) 2003-12-19
NO20044802L (en) 2004-12-08
PE20040537A1 (en) 2004-11-04
AR039938A1 (en) 2005-03-09
CN1656217A (en) 2005-08-17
MXPA04011224A (en) 2005-01-25
KR20050038593A (en) 2005-04-27
JP2005533492A (en) 2005-11-10

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MM4A The patent is invalid due to non-payment of fees

Effective date: 20090529