PE20040537A1 - MODIFIED PHYTASES - Google Patents

MODIFIED PHYTASES

Info

Publication number
PE20040537A1
PE20040537A1 PE2003000534A PE2003000534A PE20040537A1 PE 20040537 A1 PE20040537 A1 PE 20040537A1 PE 2003000534 A PE2003000534 A PE 2003000534A PE 2003000534 A PE2003000534 A PE 2003000534A PE 20040537 A1 PE20040537 A1 PE 20040537A1
Authority
PE
Peru
Prior art keywords
model
fitasa
polypeptide
seq
amino acid
Prior art date
Application number
PE2003000534A
Other languages
Spanish (es)
Inventor
Jan Metske Van Der Laan
Stefanus Cornelis Hendrikus Josef Turk
Original Assignee
Basf Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Ag filed Critical Basf Ag
Publication of PE20040537A1 publication Critical patent/PE20040537A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes

Abstract

SE REFIERE A UN POLIPEPTIDO TAL QUE CUANDO ES ALINEADO CON LA FITASA MODELO ASPERGILLUS NIGER, CONTIENE UNA MODIFICACION SELECCIONADA DEL GRUPO FORMADO DE: A) SUSTITUICION DE UN AMINOACIDO PRESENTE EN LA FITASA MODELO; B) SUPRESION DE UN AMINOACIDO PRESENTE EN LA FITASA MODELO; C) INSERCION DE UN AMINOACIDO. LA ALINEACION DEL POLIPEPTIDO PRESENTA AL MENOS DE UN 91-99% DE HOMOLOGIA Y AL MENOS ESTA MODIFICADO EN UNA DE LAS SIGUIENTES POSICIONES: 3, 78, 163, 180, 182,194, 211, 215, 242, 254, 269, 414, 428, 440. DICHO POLIPEPTIDO ES DE LA SEQ ID N°: 3, SEQ ID N°: 5 O SEQ ID N°: 7 Y POSEE LAS MISMAS PROPIEDADES FABORABLES QUE LA FITASA MODELO COMBINADAS CON UNA GRAN ESTABILIDAD Y ACTIVIDAD A TEMPERATURAS Y HUMEDAD ALTASIT REFERS TO A POLYPEPTIDE SUCH THAT WHEN ALIGNED WITH THE ASPERGILLUS NIGER MODEL FITASA, IT CONTAINS A SELECTED MODIFICATION OF THE GROUP FORMED BY: A) REPLACING AN AMINO ACID PRESENT IN THE MODEL FITASA; B) REMOVAL OF AN AMINO ACID PRESENT IN THE MODEL PHYTASE; C) INSERTION OF AN AMINO ACID. THE ALIGNMENT OF THE POLYPEPTIDE HAS AT LEAST 91-99% HOMOLOGY AND IS AT LEAST MODIFIED IN ONE OF THE FOLLOWING POSITIONS: 3, 78, 163, 180, 182,194, 211, 215, 242, 254, 269, 414, 428 , 440. SAID POLYPEPTIDE IS OF SEQ ID N °: 3, SEQ ID N °: 5 OR SEQ ID N °: 7 AND HAS THE SAME FABORABLE PROPERTIES AS THE MODEL FITASA COMBINED WITH GREAT STABILITY AND ACTIVITY AT HIGH TEMPERATURES AND HUMIDITY

PE2003000534A 2002-05-30 2003-05-30 MODIFIED PHYTASES PE20040537A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP02100608 2002-05-30

Publications (1)

Publication Number Publication Date
PE20040537A1 true PE20040537A1 (en) 2004-11-04

Family

ID=29595046

Family Applications (1)

Application Number Title Priority Date Filing Date
PE2003000534A PE20040537A1 (en) 2002-05-30 2003-05-30 MODIFIED PHYTASES

Country Status (16)

Country Link
EP (1) EP1513931A2 (en)
JP (1) JP2005533492A (en)
KR (1) KR20050038593A (en)
CN (1) CN1656217A (en)
AR (2) AR039938A1 (en)
AU (1) AU2003238448A1 (en)
BR (1) BR0311287A (en)
CA (1) CA2485513A1 (en)
MX (1) MXPA04011224A (en)
NO (1) NO20044802L (en)
PE (1) PE20040537A1 (en)
PL (1) PL372824A1 (en)
RU (1) RU2329301C2 (en)
TW (2) TW200401033A (en)
WO (1) WO2003102174A2 (en)
ZA (1) ZA200410369B (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0422052D0 (en) 2004-10-04 2004-11-03 Dansico As Enzymes
WO2007112739A1 (en) 2006-04-04 2007-10-11 Novozymes A/S Phytase variants
DE102006053059A1 (en) * 2006-11-10 2008-05-15 Ab Enzymes Gmbh Polypeptide with phytase activity and increased temperature stability of the enzyme activity and this coding nucleotide sequence
CN102854158B (en) * 2011-07-01 2016-01-20 北京昕大洋科技发展有限公司 A kind of method of Fast Measurement phytase heat resistance
CN105441406A (en) * 2014-08-05 2016-03-30 北京大学 Phytase variant
CN108251439B (en) * 2018-01-11 2021-03-30 山西大学 Artificially-modified trypsin-resistant phytase as well as preparation method and application thereof
CN117535266A (en) * 2020-04-29 2024-02-09 南京百斯杰生物工程有限公司 Parent phytase variant

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000043503A1 (en) * 1999-01-22 2000-07-27 Novozymes A/S Improved phytases

Also Published As

Publication number Publication date
ZA200410369B (en) 2006-06-28
NO20044802L (en) 2004-12-08
TW200403341A (en) 2004-03-01
AU2003238448A1 (en) 2003-12-19
CA2485513A1 (en) 2003-12-11
KR20050038593A (en) 2005-04-27
PL372824A1 (en) 2005-08-08
CN1656217A (en) 2005-08-17
WO2003102174A2 (en) 2003-12-11
BR0311287A (en) 2005-03-29
WO2003102174A3 (en) 2004-03-25
TW200401033A (en) 2004-01-16
EP1513931A2 (en) 2005-03-16
JP2005533492A (en) 2005-11-10
MXPA04011224A (en) 2005-01-25
AR039938A1 (en) 2005-03-09
RU2004139054A (en) 2005-06-27
RU2329301C2 (en) 2008-07-20
AR039939A1 (en) 2005-03-09

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