AR009477A1 - Metodo y aparato para deteccion fluorescente bicolor de molecula unica y determinacion de peso molecular y concentracion - Google Patents

Metodo y aparato para deteccion fluorescente bicolor de molecula unica y determinacion de peso molecular y concentracion

Info

Publication number
AR009477A1
AR009477A1 ARP970103935A AR009477A1 AR 009477 A1 AR009477 A1 AR 009477A1 AR P970103935 A ARP970103935 A AR P970103935A AR 009477 A1 AR009477 A1 AR 009477A1
Authority
AR
Argentina
Prior art keywords
molecular weight
concentration
determination
allows
target molecule
Prior art date
Application number
Other languages
English (en)
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Publication of AR009477A1 publication Critical patent/AR009477A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Un método para detectar la presencia de una molécula objetivo en una muestra y determinar su peso molecular y concentracion. El método involucrala determinacion de velocidades electrocinéticas midiendo el tiempo requerido para que moléculasindividuales rotuladas con por lo menos dos sondasfluorescentes se desplacen a través de una distancia fija entre dos haces láser. La comparacion de la velocidad electroforética conocida comocaracterística para los estándares de peso molecularparticulares con la velocidad electroforética de las especies objetivo permiten la determinaciondel peso molecular del objetivo. Alternativamente, un método con una unica zona deteccion y dos sondas que utiliza flujo mecánico, electrosmotico oelectrocinético de una muestra a través de un tubo de transporte que permite la identificacion de una molécula objetivo en una muestra de ensayo compleja. Elmétodo es sumamente sensible y permite detectar y determinar en cuestion de segundos el peso molecular de una molécula objetivo presente en unaconcentracion de tan solo un femtomolar.
ARP970103935 1996-09-03 1997-08-28 Metodo y aparato para deteccion fluorescente bicolor de molecula unica y determinacion de peso molecular y concentracion AR009477A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US70946896A 1996-09-03 1996-09-03

Publications (1)

Publication Number Publication Date
AR009477A1 true AR009477A1 (es) 2000-04-26

Family

ID=24849984

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP970103935 AR009477A1 (es) 1996-09-03 1997-08-28 Metodo y aparato para deteccion fluorescente bicolor de molecula unica y determinacion de peso molecular y concentracion

Country Status (4)

Country Link
AR (1) AR009477A1 (es)
AU (1) AU3829197A (es)
CR (1) CR5602A (es)
WO (1) WO1998010097A2 (es)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3935509B2 (ja) 1997-02-12 2007-06-27 ワイ． チャン，ユージーン ポリマー分析のための方法および製品
US6263286B1 (en) 1998-08-13 2001-07-17 U.S. Genomics, Inc. Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer
US6696022B1 (en) 1999-08-13 2004-02-24 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
US6762059B2 (en) * 1999-08-13 2004-07-13 U.S. Genomics, Inc. Methods and apparatuses for characterization of single polymers
US6927065B2 (en) * 1999-08-13 2005-08-09 U.S. Genomics, Inc. Methods and apparatus for characterization of single polymers
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
WO2003035671A2 (en) * 2001-10-24 2003-05-01 Singulex, Inc. Methods for detecting genetic haplotypes by interaction with probes
JP2005527220A (ja) 2002-05-28 2005-09-15 ユー．エス． ジェノミクス， インコーポレイテッド 単一のポリマー分析を使用する方法および装置
US7282330B2 (en) 2002-05-28 2007-10-16 U.S. Genomics, Inc. Methods and apparati using single polymer analysis
WO2004066185A1 (en) * 2003-01-23 2004-08-05 U.S. Genomics, Inc. Methods for analyzing polymer populations
US7595160B2 (en) 2004-01-13 2009-09-29 U.S. Genomics, Inc. Analyte detection using barcoded polymers
EP1704256A4 (en) 2004-01-13 2008-01-16 Us Genomics Inc DETECTION AND QUANTIFICATION OF ANALYTES IN SOLUTION USING POLYMERS
EP1786932A4 (en) 2004-08-23 2010-10-27 Us Genomics Inc SYSTEMS AND METHOD FOR THE DETECTION AND ANALYSIS OF POLYMERS
WO2006098772A2 (en) 2004-10-13 2006-09-21 U.S. Genomics, Inc. Systems and methods for measurement optimization
US8045140B2 (en) * 2007-10-18 2011-10-25 Stc.Unm Method for multivariate analysis of confocal temporal image sequences for velocity estimation
US9028776B2 (en) 2012-04-18 2015-05-12 Toxic Report Llc Device for stretching a polymer in a fluid sample

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993019205A1 (en) * 1992-03-19 1993-09-30 The Regents Of The University Of California Multiple tag labeling method for dna sequencing
WO1993020236A1 (en) * 1992-04-03 1993-10-14 Applied Biosystems, Inc. Probe composition and method
JP3068413B2 (ja) * 1994-07-13 2000-07-24 日立電子エンジニアリング株式会社 Ｄｎａ塩基配列決定装置
US6045995A (en) * 1994-08-24 2000-04-04 Isis Pharmaceuticals, Inc. Capillary electrophoretic detection of nucleic acids
DE19508366C2 (de) * 1995-03-10 1998-01-29 Evotec Biosystems Gmbh Verfahren zum direkten Nachweisen weniger Nucleinsäurestränge
EP0854937B9 (en) * 1995-10-06 2006-06-28 Perseptive Biosystems, Inc. Methods and kit for hybridization analysis using peptide nucleic acid probes

Also Published As

Publication number Publication date
WO1998010097A2 (en) 1998-03-12
CR5602A (es) 1998-08-31
WO1998010097A3 (en) 2002-10-17
AU3829197A (en) 1998-03-26

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