AR009477A1 - Metodo y aparato para deteccion fluorescente bicolor de molecula unica y determinacion de peso molecular y concentracion - Google Patents
Metodo y aparato para deteccion fluorescente bicolor de molecula unica y determinacion de peso molecular y concentracionInfo
- Publication number
- AR009477A1 AR009477A1 ARP970103935A AR009477A1 AR 009477 A1 AR009477 A1 AR 009477A1 AR P970103935 A ARP970103935 A AR P970103935A AR 009477 A1 AR009477 A1 AR 009477A1
- Authority
- AR
- Argentina
- Prior art keywords
- molecular weight
- concentration
- determination
- allows
- target molecule
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
Un método para detectar la presencia de una molécula objetivo en una muestra y determinar su peso molecular y concentracion. El método involucrala determinacion de velocidades electrocinéticas midiendo el tiempo requerido para que moléculasindividuales rotuladas con por lo menos dos sondasfluorescentes se desplacen a través de una distancia fija entre dos haces láser. La comparacion de la velocidad electroforética conocida comocaracterística para los estándares de peso molecularparticulares con la velocidad electroforética de las especies objetivo permiten la determinaciondel peso molecular del objetivo. Alternativamente, un método con una unica zona deteccion y dos sondas que utiliza flujo mecánico, electrosmotico oelectrocinético de una muestra a través de un tubo de transporte que permite la identificacion de una molécula objetivo en una muestra de ensayo compleja. Elmétodo es sumamente sensible y permite detectar y determinar en cuestion de segundos el peso molecular de una molécula objetivo presente en unaconcentracion de tan solo un femtomolar.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US70946896A | 1996-09-03 | 1996-09-03 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR009477A1 true AR009477A1 (es) | 2000-04-26 |
Family
ID=24849984
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP970103935 AR009477A1 (es) | 1996-09-03 | 1997-08-28 | Metodo y aparato para deteccion fluorescente bicolor de molecula unica y determinacion de peso molecular y concentracion |
Country Status (4)
Country | Link |
---|---|
AR (1) | AR009477A1 (es) |
AU (1) | AU3829197A (es) |
CR (1) | CR5602A (es) |
WO (1) | WO1998010097A2 (es) |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3935509B2 (ja) | 1997-02-12 | 2007-06-27 | ワイ. チャン,ユージーン | ポリマー分析のための方法および製品 |
US6263286B1 (en) | 1998-08-13 | 2001-07-17 | U.S. Genomics, Inc. | Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer |
US6696022B1 (en) | 1999-08-13 | 2004-02-24 | U.S. Genomics, Inc. | Methods and apparatuses for stretching polymers |
US6762059B2 (en) * | 1999-08-13 | 2004-07-13 | U.S. Genomics, Inc. | Methods and apparatuses for characterization of single polymers |
US6927065B2 (en) * | 1999-08-13 | 2005-08-09 | U.S. Genomics, Inc. | Methods and apparatus for characterization of single polymers |
WO2002044425A2 (en) | 2000-12-01 | 2002-06-06 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
WO2003035671A2 (en) * | 2001-10-24 | 2003-05-01 | Singulex, Inc. | Methods for detecting genetic haplotypes by interaction with probes |
JP2005527220A (ja) | 2002-05-28 | 2005-09-15 | ユー.エス. ジェノミクス, インコーポレイテッド | 単一のポリマー分析を使用する方法および装置 |
US7282330B2 (en) | 2002-05-28 | 2007-10-16 | U.S. Genomics, Inc. | Methods and apparati using single polymer analysis |
WO2004066185A1 (en) * | 2003-01-23 | 2004-08-05 | U.S. Genomics, Inc. | Methods for analyzing polymer populations |
US7595160B2 (en) | 2004-01-13 | 2009-09-29 | U.S. Genomics, Inc. | Analyte detection using barcoded polymers |
EP1704256A4 (en) | 2004-01-13 | 2008-01-16 | Us Genomics Inc | DETECTION AND QUANTIFICATION OF ANALYTES IN SOLUTION USING POLYMERS |
EP1786932A4 (en) | 2004-08-23 | 2010-10-27 | Us Genomics Inc | SYSTEMS AND METHOD FOR THE DETECTION AND ANALYSIS OF POLYMERS |
WO2006098772A2 (en) | 2004-10-13 | 2006-09-21 | U.S. Genomics, Inc. | Systems and methods for measurement optimization |
US8045140B2 (en) * | 2007-10-18 | 2011-10-25 | Stc.Unm | Method for multivariate analysis of confocal temporal image sequences for velocity estimation |
US9028776B2 (en) | 2012-04-18 | 2015-05-12 | Toxic Report Llc | Device for stretching a polymer in a fluid sample |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1993019205A1 (en) * | 1992-03-19 | 1993-09-30 | The Regents Of The University Of California | Multiple tag labeling method for dna sequencing |
WO1993020236A1 (en) * | 1992-04-03 | 1993-10-14 | Applied Biosystems, Inc. | Probe composition and method |
JP3068413B2 (ja) * | 1994-07-13 | 2000-07-24 | 日立電子エンジニアリング株式会社 | Dna塩基配列決定装置 |
US6045995A (en) * | 1994-08-24 | 2000-04-04 | Isis Pharmaceuticals, Inc. | Capillary electrophoretic detection of nucleic acids |
DE19508366C2 (de) * | 1995-03-10 | 1998-01-29 | Evotec Biosystems Gmbh | Verfahren zum direkten Nachweisen weniger Nucleinsäurestränge |
EP0854937B9 (en) * | 1995-10-06 | 2006-06-28 | Perseptive Biosystems, Inc. | Methods and kit for hybridization analysis using peptide nucleic acid probes |
-
1997
- 1997-08-08 WO PCT/US1997/013938 patent/WO1998010097A2/en active Application Filing
- 1997-08-08 AU AU38291/97A patent/AU3829197A/en not_active Abandoned
- 1997-08-26 CR CR5602A patent/CR5602A/es not_active Application Discontinuation
- 1997-08-28 AR ARP970103935 patent/AR009477A1/es not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
WO1998010097A2 (en) | 1998-03-12 |
CR5602A (es) | 1998-08-31 |
WO1998010097A3 (en) | 2002-10-17 |
AU3829197A (en) | 1998-03-26 |
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Legal Events
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FA | Abandonment or withdrawal |