JPH11240827A - Preparation composition for external use containing gamma-polyglutamic acid and vegetable extract in combination - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain the subject composition capable of increasing pyrrolidonecarboxylic acid on a skin epidermis in a safe and natural process and enhancing the humectant effects without disturbing the quantitative balance and constancy of a humectant ingredient essential to the skin by prescribing polyglutamic acid and a vegetable extract in combination. SOLUTION: This composition is obtained by prescribing (A) a γ-polyglutamic acid prepared by culturing, e.g. a strain of Bacillus subtilis in a synthetic culture medium or its salt and (B) a vegetable extract obtained by extracting, e.g. a plant such as Aloe vera, Matricaria chamolilla L. or green tea with an aqueous solution of ethanol. The amounts of the prescribed ingredients are preferably 0.01-2.0 wt.% ingredient A and about 0.001-0.5 wt.% ingredient B expressed in terms of the solid content in combination. The composition is useful as a medicine for external use, a quasi-drug, a cosmetic, a bathing agent, daily sundry goods, or the like.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a composition which, when used externally, promotes the production of pyrrolidone carboxylic acid on the skin surface and effectively acts to prevent, reduce or improve skin dryness, rough skin, inflammatory diseases and the like. It is about things. The external preparation composition according to the present invention is an external medicine,
It means various preparations for local or systemic use, such as quasi-drugs, cosmetics, bath preparations, daily miscellaneous goods, etc., or thrown into bath water.

[0002]

2. Description of the Related Art Human skin is covered with a stratum corneum, a thin biological defense membrane, and can live without loss of water even in a dry atmosphere because of the stratum corneum in contact with the outside world. Because it exists. The stratum corneum is thin and supple, is kept so as not to lose moisture in the body, and is adjusted to maintain a healthy skin condition.

[0003] However, due to environmental factors and the like (for example, temperature change, humidity change, light, contact with water, etc.),
Often, the epidermis causes some change or hindrance. The skin that has reached the functionally incomplete stratum corneum is hard, loses elasticity, and becomes crunchy. Such dry skin has also been pointed out to be related to atopic dermatitis, which has been rapidly increasing in recent years, and may cause serious skin troubles.

Conventionally, plant extracts and oils and fats have been used in external preparations such as cosmetics to prevent dry skin.
The purpose of these is to moisturize the skin, create a fat film to prevent the transpiration of water in the stratum corneum, supplement the intercellular lipids, etc. Was to prevent it.

[0005] However, plant extracts are not satisfactory in terms of the continuation of the moisturizing effect due to the provision of temporary moisture, and the effect of fats and oils components cannot be expected if the amount used is small, and conversely, large amounts of oils and fats cannot be expected. Over time, the skin becomes greasy,
There is a disadvantage that it gives a sticky feeling.

On the other hand, based on the idea that it is ideal to apply and reproduce on the skin a substance substantially similar to the components on the skin surface, a natural moisturizing factor: NMF (Natural Moisturizing Fac
The stratum corneum component called tar) has been attracting attention. The natural moisturizing factor (hereinafter referred to as NMF) includes those composed of amino acids, organic acids such as lactic acid and salts thereof, pyrrolidonecarboxylic acid, urea, saccharides and the like.

[0007] This NMF is an important component for the regulation function of the stratum corneum of the skin because it makes the stratum corneum of the skin hydrophilic and takes in moisture and has a water retaining action. In particular, pyrrolidone carboxylic acid accounts for about 12% by weight of the total NMF,
Since it has a high hygroscopicity as compared with other components in MF, it is considered to be a very important substance in maintaining skin moisture retention.

However, pyrrolidone carboxylic acid is eluted by prolonged contact with water or the like, and pyrrolidone carboxylate salts which are said to be relatively safe (eg, sodium pyrrolidone carboxylate, potassium pyrrolidone carboxylate, ethanol pyrrolidone carboxylate) Amine)
It has been reported that erythema occurs when a highly concentrated solution is applied to the skin. Therefore, it has been pointed out that continuous heavy application of pyrrolidonecarboxylic acid or a salt thereof to skin epidermis may cause chronic skin inflammation.

[0009]

SUMMARY OF THE INVENTION In order to solve the above-mentioned problems, the present inventors have increased pyrrolidone carboxylic acid on the skin epidermis by a safe and natural process to achieve a quantitative balance of the moisturizing components inherent in the skin. We have been researching and developing ingredients that enhance moisturizing effects without disturbing homeostasis.

[0010]

As a result, by applying γ-polyglutamic acid (hereinafter referred to as γ-PGA) or a salt thereof on the skin epidermis, the amount of pyrrolidonecarboxylic acid on the skin surface is increased. It was found that the amount and rate of increase were improved by using a plant extract having a moisturizing effect in combination with it, and it was found that the moisturizing effect was maintained. Agent composition was completed.

Hereinafter, the pyrrolidonecarboxylic acid and its production reaction will be described in order to better understand the present invention.

[0012]

Embedded image As shown in Chemical formula 1, the formation reaction of pyrrolidone carboxylic acid (sodium pyrrolidone carboxylate) in a living body has not been elucidated in many cases, but generally, when a protein is degraded along with keratinization in the epidermis and stratum corneum. It is thought that free amino acids are involved. That is, glutamic acid, one of the free amino acids, is converted to the enzyme (γ-glutamyl-A
A-synthetase, γ-glutamyl-cyclotransferase), ring closure, dehydration and conversion to pyrrolidone carboxylic acid by heat, heat and the like, and furthermore, most of the skin epidermis is weakly acidic (pH 5.3 to 5.6). It is believed that the pyrrolidone carboxylic acid is in the form of a sodium or other salt.

Therefore, by directly applying γ-PGA or a salt thereof to the skin, it gradually penetrates into the stratum corneum of the skin, is decomposed into glutamic acid by the action of a protease, and the like. It is presumed that the reaction proceeds gently in the epidermis and stratum corneum of the skin, and pyrrolidone carboxylic acid is spontaneously produced in a safe range in an appropriate amount. In addition, it is considered that it is metabolized to sodium pyrrolidonecarboxylate and other salts under weak acidity on the skin epidermis, and regulates the skin to a normal state.

Although the reason why the production of pyrrolidone carboxylic acid is further enhanced by the combined use of the plant extract is not yet known, it is supposed that the skin surface condition is improved by moisturization,
It is presumed that this is due to the improvement of biological functions such as enzyme activity.

[0015] γ-PGA or a salt thereof used in the present invention can be produced by a known method utilizing a microorganism capable of producing γ-PGA. Available microorganisms include:
A strain belonging to the genus Bacillus is preferable, and Bacillus subtilis or a variant thereof, Bacillus subtilis.
Anthracis (Bacillus anthracis), Bacillus licheniformis, Bacillus megaterium (Bacillus megaterium), Bacillus natto (Bacillus natto) and the like.

As a method of producing by culturing in a synthetic medium, Japanese Journal of Agricultural Chemistry 37 (8), pp. 474-477 (1963),
43 (9), pp. 595-598 (1969), and the method described in JP-B-43-24472 can be used. In addition, it can be extracted and purified from a viscous substance of natto commercially available, as well as a culturing method in a shochu distillation waste liquid medium (Japanese Patent Application Laid-Open No. 8-308590).

When extracting from natto, natto (1.0 kg)
Add purified water (5 L) to the mixture and gently stir to dissolve the viscous matter on the natto surface. Next, the insoluble matter is removed by centrifugation or filtration, and the precipitate obtained by adding the solution to ethanol is recovered. If necessary, the precipitate is dissolved in water and subjected to ultrafiltration or dialysis to remove low molecular substances. Thus, the composition containing a high content of γ-PGA (about 10 to 40 g)
obtain.

As the plant extract as a concomitant component, any extract having a moisturizing action derived from a plant as shown below can be used. Specific plants (herbal medicines) include, for example, almonds
Ai (Aiba), Aokazura (Seifu Fuji), Aotsu-la-Fuji (Kibo), Aono Ryu-Getsuran, Fukurin Ryu-Getsuran, Acacia, Akasuri Fruit, Aka-grape, Akamegawiwa (Akabu-Kashiwa), Akane (Akane-Kusa), Aka-ya-Jiou
(Jihuang), Agi (Awei), Akinire (Luyu bark), Akebi (Kidori), Asa (Jinji), Malva Asagao or Asagao (Toshiko), Ashitaba (Tomorrow's leaf), Azuki (Red red bean), Acerola ,
Asenyaku (Asenyaku), Anise, Abemaki fruit, Avocado, Ama, Amacha (sweet tea), Amachaworu, Amadokoro
(Tamatake), Amigasa lily (Mother), Altea, Arnica, Aloe (lux), Aloe vera, Angelica, Anzu Honganzu (Anjin), Ansokkou (Azuka), Igakozolina
(Bile head), knotweed (tiger cane root), strawberry, fig (fructose) or its leaves, strawberry (winter aoiko), ginkgo biloba, ginkgo (ginkgo biloba, ginkgo), Itohimehagi (enshi), carob, rice seed or seed coat , Inondo seeds, Ibuki gypsophila, nettle, ylang ylang, fennel (Fenka), ukiyagara (sanryo), guaysukagra fruit, Himeuikyo, turmeric (Utsukin), Usubasaishin Keirinsaishin
(Spicy), Usbania Oyster, Usbenitachioi, Utsubogusa (summer hay), Udo or Shishiudo (Qang-act, Independent, Tang-do), Ume (Ume) or its pulp, Vladigashi, Oolong tea, Uwaurushi, Unshumikan (Chin), Estragon , Ezokogi (Ezo Goka), Ezoki strawberry, Echinashi (Hosobamurasakibarengiku), Enokitake (Enotake),
Ebisugusa (Keiko), Elderberry fruit, Elemi, Elingy (Pre-Lotus Elinge)
Sophoraceae (rice soybean), ogi / kibanaoogi (radix astragali), ouren (orchid), giant crow squirrel (caronin), giant elephants (self-defense), psyllium (vegetative child, vegetative grass), toucanashimi (haruko), and eucalyptus okaera White jellyfish), Obayashi shabushi fruit, O hawthorn hawthorn (Yamasato), Oohisasanoyuki, Sasanoyuki, barley (barley), Ogurma (turning over), Okra fruit, Panax ginseng
(Ginseng), St. John's wort, St. John's wort, St. John's wort
(Hihayu), Ominaeshi (losing sauce), Dutch strawberry, Dutch mustard (Watercress), Dutch jelly, Dutch honeybee,
Olive, oregano, orange fruit or peel, silkworm (ripe yellow), cacao seed, oyster or its leaves (persimmon),
Kagikazura (Hook of Choto), Cassia, Cassinaki fruit (Kozomi), Gajutsu (Shazajutsu), Kashiwa (Kazuki), Cascarilla, Kanixa (Kinosato), Kanokosou (Kikusone), Birch or Shirakaba (Shirakaba) , Squash, capoknoki seeds, kahokusansho (shopper), cattail (bamboo), chamomile and roman chamomile, kamiyatsude (weeds), crow squirrel (winter melon), rasabisaku (midsummer), oats mulberry, guarana seeds, karahoo ), Karaya, Karin (Gourd Gourd), Garcinia, Kawayanagi, Kawaratake, Kawamuragi (Inchinko), Kansui
(Sweet), licorice (licorice), cantaraasa, candelilla,
Kantou, Canna, Raspberry, Kiwi fruit, Kikarasuuri (Ururone), Kikyo (Kikyo, Kikyo root), Kiku (Chrysanthemum flower), Kisasage (Azumitsu), Rabbits (Yotei root), Pheasant (Kizumi), Kizuta, Kidachi aloe , Yellow mint, Kinna, Yellow linden (Shincona, Red linden), Yellowfin (Huangkashii), Gymnema
Sylvesta, cabbage, immature cabbage, cucumber, Kiraja saponaria, Kiraya, kumquat fruit, betel,
Agrimony (Sesangaku), Guava Fruit, Guayule, Quercus Infectoria (Gallery), Kukui Nuts, Kuko (Gugi, Gyugia, Gygi Leaf, Skeletal Bark), Kusagi Kazura
(Tenmen winter), Kuzu (Kakkon), Camphor tree, Gooseberry fruit,
Gardenia, gardenia, black clover, kumazasa, black pine, horseradish, clara, bitter cranberry fruit, chestnut or its astringent skin, curculigo latifolia fruit, grapefruit, buckthorn, black crocodile (rescue),
Kurobana Hikikoshi, cloves (cloves, cloves), kayai
(Thorn tree, Thorn tree ear), Celosia (rooster corolla, rooster), Bay laurel (laurel), Kenashisarutoribara (Tokubukuryo, mountain return), Gentiana, Gennoshoko (Old grass), Koshinbara (Monthly flower), Kou-suihakka, Kozo Fruit, Kocha
(Tea), kouhone (river bone), kouhon (straw and kara straw), kouriang, koendro fruit, koganebana (yellow gon), lingonberry (koshibachi), coconut fruit, goshyu (goshuyu), goshoichigo (covered bonsai), Pepper, peppercorn balsam,
Coffee beans, kobushi magnolia, burdock,
Burdock), kobotdur, sesame (sesame), sesame seeds (gensin), beetle (gomiko), Sanekazura binankazura.
Matsubusa, wheat (wheat), rice, rice bran (red bran, white bran), rice oil, cola acuminata seed, cola vera seed, fenugreek fruit, colombo, conzulango, kelp, konjac, comfrey (finnari grass), sisal , Sisal Asa, Sakisima Button (Sacred Spirits), Sakura (Oshimazakura,
Leaves, flowers, fruits, bark (cherry bark), cherries, yamazakura, oyamazakura, edhigashi, mamezakura, miyamazakura, yoshino cherry tree, takanezakura, kasumizakura, chojizakura, kohigan, satozakura, kanzakura)
Pomegranate, Sasa, Sazanka, Sadomodaka (Sawaka), Sweet potato, Sugar cane, Sugar beet, Sunabet jujube
(Acid jujube), saffron (red safflower, western safflower), pomelo fruit, savonso, salmonberry fruit, sarasinosa (maso), salvia (sage), sawagikyo (yamachona), sawagurumi (yamawomo), sanshinjinjin (37 ginseng), sanshuyu (sanshuyu), sansho (sansho), sanzukon (yamazune), shea (carite), cyanoki fruit, shiitake mushroom (shiitake),
Shion, Shikitsuri, Shikunshi, Shiso
Aodiso, Chirimenzo, and Catamediso (Shiyo-lea, Shiso), Rosewood, Linden, Chinese pine, Chinese Forsythia (Forsythia), Simakangi (Chrysanthemum), Shimeji (Hinshimeji,
Shakashimeji, Hatakeshimeji, Oshiiroshimeji, Bunashimeji, Shirotamogitake),
Peonies (Shakuyaku), Shajin (Sasan), Janohige (Bakumon winter), Juzudama, Palm fruit, Ginger (Ginger), Shoubu (Iris, Iris root), Shozuku fruit, Shirakashi seed, Shirogoshi butterfly, Shirobanairis, Shirobanatsuta Flower, Shirominanten (South Tenmi), Cinchona Saksilbra,
Cinnamon, watermelon (watermelon), honeysuckle (gold and silver flowers, shintouyu), sorrel (acidic model), swimberry fruit, stevia, strawberry fruit, horsetail (thorn), stevia, plum fruit, pine cone, pine larch, pine Kizuta, Walnut walnut, Atlantic hawthorn, Dandelion dandelion, Horse chestnut (Marronnier), Pear fruit, Prunus officinalis, Atlantic elderberry (Elder), European elder (Juniper / Morimatsu), European saw palm millet , Mistletoe, common mint, common mint, common horseradish, common horseradish, red croaker (stone iris), common mallow, hirohasenega, senega, celery, celery, senkyu (river cucumber), sendan, senburi (medicine), senna fruit or leaf, senna Ginseng (great tate), soka (grass), buckwheat seed, soybean potato, rhubarb (daihuang), radish, soybean, daidai (orange peel, kikomi), takatodai (daigei), takarababi (gull), dark sweet cherry fruit , Hollyhock, lycopodium (thyme, momoka), hollyhock (tachibana skin), hollyhock, tamarind seeds,
Onion, tamashiba (hot spring), arachis or its root bark, tanjin (dansin), dandelion (gakkohide) or shirobana dandelion, moko dandelion, dammar, cherry fruit, tigaya or its root (chinese root), chikusetsujin (bamboo ginseng), Chicory, Korean gourd (Gomiko), Korean radish
(Ohta), Korean ginseng (Wuyi), Korean ginseng
(Ginseng), Artemisia (Aiba), Choreimaitake
(Irei), Japanese Ginseng, Japanese chestnut (mushroom),
Camellia, camellia, clover (lacquer lacustrine), communis (duckweed), vines (red azuki), vines (red bean), vines (vegetables), vines (four-leaf ginseng), vines, deiko, teuchigurumi, duberry fruit, tengusa , Tendai yak (crowd medicine), Pepper (winter gourd), Pepper (bansho),
Touki (Toki), Calendula (marigold), Corn or corn hair (Nanban hair)
(Sadako), Toudondou (Ryubile), Dokudami (Jyukuyaku), Tokon (Sperm Root), Toshishi Mamedaoshi Nennashikazura, Tochu (Tonaka, Tonakaha), Tomato, Traganto, Triacanthus Seed, Tormentilla, Doronoki, Troroaoi, Niseria berry fruit, Chinese yam (Yamanoimo), Naked ramen (Butcher bloom), Naginata koju, Nazuna, Rape seed, Natsumikan, Jujube (daijujutsu), Naniwaibara
(Kanesakurako), Nameko, Narco lily (Yellow), Peanut
(Peanut), Nanten (Minami Tenmi), Nigaki (Maki), Nyagamogi (Grass), Nikuzuku, Kay Nikkei (Caesar) / Keishi (Keeshi), Chive (Nirako), Elderberry (Grasswood)・ Flowers or foliage, garlic (garlic), nurde (fivefold), leek, thistle (daiji), wild rose (fruit), yarrow, sawdust
(Mae Hu), Novara, Nomomo, Palm, Pineapple Fruit,
Hibiscus (Bussouge, Furin-Bussouge, Roselle), Hakamauraboshi (Bone Crushing Supplement), Hachsen (Ringworm Skin),
Huckleberry fruit, chickweed (red), hazel (haruko), hasidoro (roth root), basil, lotus (lotus, lotus root), parsley (holland jelly), hamadamugi, batata, hachiku madake (bamboo-boiled), patchouli, mint (Light load, light load leaf), barley (yokuinin), hanasuge (chimbo), banana, hanahacka, vanilla bins, papaya, hakogusa (mouse asparagus), hub grass, paprika, hamago and honeysuckle (crop)
Hamasuge (Kazuki), Hamabishi (Sitsuriko), Hamanas (Maikai flower), Hamaboufu (Hamaboshi), Hamamelis, Rose
(Rose), agaric (aratake, white agaricus, usukimori no kasa), parietaria, harunire (yellow, yu white skin, yuba), breadfruit, giant goose (shadow), lycopodium carrot (partisan), pecan nut, giganbana (stone garnish) , Suzuka Suzuka), Hikikoshi (prolonged life grass), Hishi (Hishimi), Pistachio, Beet, Hitotsuba (Ishire), Hinata no Kozuchi (cow knee), Hinoki, Hiba, Castor, Sunflower, Bell pepper, Himegama , Himematsutake (Kawariharatake, Hiromatsutake), Pimenta fruit, Juniper, Bjakuyu, Hiyu fruit, Beloyd mallow, Hiroha okinagusa,
Loquat (loquat, loquat leaf), betel nut (large clothing skin, betel loach), sprout beetle (southern rattan), butterflies, coltsfoot (poor winter flowers, poisonous winter leaves), fujibakama (orchid grass), fuji bean (flat beans), grape fruit or pericarp. Seeds / leaves, beech, sycamore (caterpillar), Brazilian liquorice, black currant fruit, blackberry, plum fruit, furcellaria, blueberry (Prunus aegypti), prunes, blonde psyllium, bundou (green beans), loofah, safflower (safflower),
Henneken, belladonna, berry fruit, Peruvian berg, Boysenberry fruit, boufu (windbreak), spinach, physalis (Torone), honoki (wakopoku, park), bokeh (gourd), hosobananaokera (blue jitsugaku), hosobabarengiku, bodaiju ( Bodai tree), button (peony, peony bark), hop, jojoba, white berry fruit, hon-shimeji, maisurutennansha (tennan star), maitake (maitake mushroom), ephedra (maoh), macadamia nut, macri (seaweed) , Magwa (mulberry white skin, mulberry leaf), magnolia splengeri, matabi (mokuten tate), matsukasa, matsuha, matsuhodo (bukuryo), mayolam (hanahanakka), malvanojazine (bitan), mulberry fruit,
Quince, mango, mangosteen, manshou walnut, mandarin fruit, mannenthus mushroom (reishi), citrus fruit (kokomi), mishimasaiko (saiko), mizokakushi (half-side lotus), misohagi (senquana), honeybee, honeybee, Green peppermint, mimosa, mioga, miracle fruit fruit, myrrh, mirobaran, mukuge (wooden geese), mukunoki, mukuroji, murasaki (purple root), murasaki maize, swordfish
(Mother grass), mebogi, melaleuca, melissa, melilot, melon fruit, sagebrush, mosouchiku, mokoko (wood incense), maple blossom, peach leaf (peach leaf), fruit
Seeds (peach kernels), bean sprouts, morello cherry fruits, moroheiya
(Burlap), yakawamaki yam, yakuchi (masuti), cornflower mackerel (cornflower), cornflower mint, yashabushi (arrow wheel), yachiyanagi, yatsude (octagonal lantern), mistletoe (willow parasite), willow leaf, yabugarashi, yabukouji (Purple gold beef), pokeweed (commercial land), alderwood (yamaharu), yamamomo (yangbai skin), sagebrush, eucalyptus, yukinoshita (tiger ear grass), yucca flebifolia, yuzu fruit, lily, yoroinga, european strawberry, mugwort ( Aoi leaf), lime fruit, rye, lacanca fruit, raspberry leaf / fruit, lavender, longan (longan meat), ryocha (green tea), apple fruit, gentian, lubas suavisimus (tenryu), lettuce, red currant fruit, lemon fruit , Lemongrass, forsythia, astragalus, wax palm, loganberry fruit, logwood, rosemary Over (Rosmarinus officinalis), rose hip (Novara), wasabi, is raised, such as burnet (Chinire).

These can be processed in a conventional manner according to the type and form of the product to be added (eg, pulverization, milling, washing, hydrolysis, fermentation, purification, pressing, extraction, fractionation,
Filtration, drying, pulverization, granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc.
Any material may be arbitrarily selected from various materials.

The solvent to be used for the extraction may be selected in consideration of the purpose and type of the product to be provided, or the processing to be performed later, but usually, water or a water-soluble organic solvent (eg, ethanol) , Propylene glycol, 1,3-butylene glycol, etc.).
However, when the content of the organic solvent is not preferable depending on the application, only water may be used, or ethanol which is easily removed after the extraction may be used, and may be used alone or in an arbitrary mixed solution with water. Exploited extract may be used.

When the plant extract is used in a systemic or topical external preparation or cosmetic, in addition to the effects of the present invention, it protects the skin and hair, improves the feel and texture, imparts flexibility, reduces irritation, Alleviation of stress by fragrance, activation of cells (prevention of cellular aging), suppression of inflammation, improvement of skin and hair quality, prevention and improvement of rough skin, hair growth, hair growth, prevention of hair loss, imparting gloss, cleaning effect, relaxation of fatigue In addition to cosmetic effects such as blood flow promotion, warm bath effect, etc., effects such as fragrance, deodorization, thickening, preservation, and buffering can be expected.

The concentration of the essential components in the external preparation composition according to the present invention varies depending on the nature of the preparation (frequency, amount, method of use, etc.) and is not particularly limited. However, usually, in γ-PGA or a salt thereof, 0.001 to 5.0% by weight, preferably 0.01 to 5.0% by weight.
To 2.0% by weight, and in the case of a plant extract as a concomitant component, 0.0001 to 1.0% by weight as a solid content, preferably 0.001 to 1.0% by weight.
It is considered that about 0.5% by weight is appropriate.

The external preparation composition of the present invention may optionally contain, in addition to the above essential components, the following components and additives used in pharmaceuticals, quasi-drugs, cosmetics, bath agents, and the like, if necessary. It can be manufactured by selecting and using together.

(1) Various fats and oils Avocado oil, almond oil, fennel oil, perilla oil,
Olive oil, orange oil, orange luffer oil, sesame oil, cocoa butter, chamomile oil, carrot oil, cucumber oil, tallow fatty acid, kukui nut oil, safflower oil, shea butter, soybean oil, camellia oil, corn oil, rapeseed oil,
Persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, cocoa butter, palm oil, palm kernel oil, mocro, coconut oil, tallow, lard, hydrogenated products of these fats and oils (hardened oil) Etc., etc.

(2) Waxes Beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax,
Montan wax, shellac wax, etc.

(3) Mineral oils Liquid paraffin, vaseline, paraffin, ozokeride, ceresin, microcrystalline wax, squalene, squalane, pristane and the like.

(4) Fatty acids Natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, isononanoic acid, caproic acid , 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2
-Synthetic fatty acids such as ethylhexanoic acid and isopentanoic acid.

(5) Alcohols Ethanol, isopiranol, lauryl alcohol,
Natural alcohols such as cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, and phytosterol; and synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, and 2-octyldodecanol.

(6) Polyhydric alcohols Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol , Polypropylene glycol, 1,3-butylene glycol, glycerin, pentaerythritol, sorbitol, mannitol and the like.

(7) Esters Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyl dodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, Myristyl lactate, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, and the like.

(8) Metal soaps Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate and the like.

(9) Gum, saccharides and water-soluble polymer compounds Arabic gum, benzoin gum, dammar gum, guaiac oil, Irish moss, karaya gum, tragacanth gum,
Carob gum, quinseed, agar, casein, lactose,
Fructose, sucrose and its esters, trehalose and its derivatives, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or hydroxyalkyl (C2-C4) to which alkylene (C2-C4) oxide such as ethylene oxide is added. ) Chitin or chitosan, low molecular weight chitin or chitosan, chitosan salts, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid and its salts, hyaluronic acid and its salts, chondroitin sulfate and its salts, heparin, ethyl cellulose, methyl cellulose, carboxy Methylcellulose, carboxyethylcellulose, sodium carboxyethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, nitrocellulose, crystalline cellulose, Vinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylic acid salts, polyalkylene oxide or crosslinked polymer thereof such as polyethylene oxide or polypropylene oxide, carboxyvinyl polymers, such as polyethyleneimine.

(10) Surfactant Anionic surfactant (alkyl carboxylate, alkyl sulfonate, alkyl sulfate, alkyl phosphate), cationic surfactant (alkyl amine salt, alkyl quaternary ammonium salt) ), Amphoteric surfactant:
Carboxylic acid type amphoteric surfactant (amino type, betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate type amphoteric surfactant, nonionic surfactant (ether type nonionic) Surfactants, ether ester type nonionic surfactants, ester type nonionic surfactants, block polymer type nonionic surfactants, nitrogen-containing nonionic surfactants), other surfactants (natural surfactants) , Derivatives of protein hydrolysates,
Polymer surfactant, surfactant containing titanium and silicon,
Fluorocarbon surfactants).

(11) Various vitamins Vitamin A group: retinol, retinal (vitamin A
1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (vitamin B1), riboflavin (vitamin B2), pyridoxine (vitamin B)
6), cyanocobalamin (vitamin B12), folic acids,
Nicotinic acids, pantothenic acids, biotins, choline,
Inositols, vitamin C group: ascorbic acid and its derivatives, vitamin D group: ergocalciferol (vitamin D2), cholecalciferol (vitamin D)
3), dihydrotaxerol, vitamin E group: tocopherol and its derivatives, ubiquinones, vitamin K
Group: phytonadione (vitamin K1), menaquinone (vitamin K2), menadione (vitamin K3), menadiol (vitamin K4), other essential fatty acids (vitamin F), carnitine, ferulic acid, γ-oryzanol, orotic acid, vitamin Ps (Rutin, eriocitrin, hesperidin), vitamin U, etc.

(12) Various amino acids Valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid , Hydroxylysine, arginine, ornithine, histidine, and the like, and their sulfates, phosphates, nitrates, citrates, and amino acid derivatives such as pyrrolidone carboxylic acid.

(13) Seaweed extract Green algae: Chlorella vulgaris, Chlorella pyrenoidsa, Chlorella ellipsoidia, Aonori (Usbaaonori, Sujiaonori, Hiraaonori, Bouaonori,
Hosoeda anonori), brown algae: Kombu (macomb, lithicum, hosomebu, Mitsuikonbu), seaweed,
Hirome, Aowakame, Giant Kelp (Macroscystis pilifera, Macrocystis integrifolia, Neocitys Luetokeana), Hijiki, Hibamata, Red algae: Hijirimen, Maxa (Tengusa), Hirakusa, Onixa, Obakusa, Katawakusa, Yatabegusa, Yuikiri, Shimantengusa, Tosakanori, Togekirinsai, Akumasakirinsai, Kirinsai, Byaksinrinsai, Tsunomata, Obatsunomata, Tochaka (Yahatsutsunomata), Ezotsunomata, Togetunomata, Hirakotonogi, Kotojinonotashita, Kotojinonotashita
Sujiusbanori, Hyusbanori, Akamomininori and the like are listed as representatives.

Other algae such as green algae (Chlamydomonas: Chlamydomonas, Akayukimo, Dunaliella: Dunaliella, Chlorococcus: Chlorococcus, Kwanomimo: Kwanomimo, Volvox: Ohikawawari, Volvox, Palmera, Yotsumemo, Spirogyra: Hizaori, Spirogyra, Tulsimidro, Hibimidro: Hibimidoro, Aosa: Anaoasa, Amiosa, Nagaosa, Kawanori: Kawanori,
Genus Fricciella, genus genus: medusa, asamidori ogusa, kawashiogusa, marimo, valonia: tamagobaronia, tamabaronia, magnum: tamatamamo, iwata: husaiwata, suricogita, heraiwirata, crocodile, crocus Sakibutimimil, Nagamil, Hiramil, and Casanori:
Casa nori, Juzumo
Mizojuzumo, Mizuazumimo, Korekaete, Tsutsumimo, Kikkogusa: Kikkogusa, Hitoexa: Hitoexa, Hirohanohiegusa, Usuhitoexa, Motsuhitoe, Sayamidro, Genus spp.

Blue-green algae (Genus L .: L., L., L., L .: L., mushrooms,
(Hassai, Yuremo, Racenmo (Spirulina) genus: Spirulina, Trichodesmium (Aiacacio), etc.).

Brown algae (Piraella spp .: Piraella spp., Shiomidoro spp .: Naganami spp., Isogrape spp .: Isogrape,
Genus Isogawara, genus Isogasula, genus genus: Gunsenkurogashira, genus Kashirazaki, genus Kashirazaki, genus Muchimo: genus Muchimo, Hiramutimo, Kebelligsa, genus Amis. Artemisia spp.
Genus Matsumo: genus Matsumo, genus matsumo: genus sylvestris, genus okinawa, genus okinawa-mozuku, genus niseftomozuku: niseftomozuku, futomozuku: genus futomozuku, ishimozuku: ishimozuku, kuromo: kuromo, nisemozuku: genus, spruce, spruce Ishige, Irolo, Icmegasa: Ichimegasa, Keyari: Keyari, Umibos: Umibos, Urushigusa: Urushigusa,
Species of the genus Coleoptera, Tobacco planta, Laminaria genus: Laminaria genus: Habanomodo genus: Habanomodo genus, Habanori genus: Havanori, genus Habanori: genus Habanori, genus bukuro: common bukuro, Ezobukuro genus: Ezobukuro, Fukuronori genus: Fukuronori, Watanabe Genus Kagomenori: Kagomenori, Muraridori: Murachidori, Shakusugusa: Sharks,
Genus Genus: Genus Genus, Genus Genus: Genus Genus: Genus Genus, Genus Kayamonori, Genus Kayamono, Genus Genus: Genus Ligustrum, Genus Tsurumo: Genus Turrum, Genus Anana: Genus Anastasia, Genus Ligustrum: Sujime, Genus Missiscoma: Genus Genus Ligustrum, G. , Onikombu, Goheikombu, Nagakombu, Pea kelp, Ochimikombu, Torokombu genus: Torokombu, Antokume genus: Antokume, Kajime: Kajime, Tsuruarame, Kurome, Kikuishikomu: Genus Kikuishi, Nejirekomu: Neshilecomb, Kuroshiomeko : Nekoshikambu,
Alame: Alame, Ainuwakame: Ainuwakame, Chigaiso, Oniwakameezoishige: Ezoishige, Yabanemoku: Yabanemoku, Rapamoku: Rapamoku, Jiromoku: Uganomok, Jiromoku, Hiemomoku, Honamomaku, Tamamomaku, Tamamomoku Akamoku, Shidamoku, Hondawara, Nejimoku, Narasamo, Mametawara, Tatsukuri, Yasumatamoku, Umitranoo, Oobamoku, Fushizushimoku, Hahakimokum, Togemoku, Jolemoku, Saw-mokum, Obanoki-moku, Genus genus: Genus
Bulukimo, genus cynomolgus: cynomolgus etc.).

Red algae (Genus genus Ushikenori: Ushikenori, Funorinoushikei, Genus Amanori: Asakusanori, Susabinori,
Genus Upirinori, Onimanori, Tasa, Filitasa, Benitasa, Rhodocorton: Milnoveni, Akebonomozuku: Akebonomozuku, Konahada: Haikonahada, Yogorekonahada, Aokonahada, Sea Species: Acer genus : Larch, Fusanori: Fusanori, Nisefusanori: Nisefusanori,
Sodegarami: sodegarami, rattles: rattle, hiraragara, hirohatamataki: hirohatamatamadaki, tamamaidaki: tamaitadaki, kakenokenori: kaginori, kakenenori, tengusa, kurobana, kurobana, swordfish, swordfish, squirrel Genus: hibiroudo, himehibiroudo,
Genus genus: genus isensis, genus genus:
Genus spruce, Ryumonsou: Ryumonsou, Hera Ryumon, Nisekarequigusa: Nisekarequigusa, Okitsubara genus: Obaokitsubara, Akaba: Akaba, Malvaacaba, Naminohana: Genus Hosobanamino, Naminohanagosa, Coralthus Genus Ikawanokawa, Genus Kanokawa: Kinokawa, Genus Kaninote: Kaninote, Coralum: Coralum, Genus Mucadenori: Mucadenori, Sujimukade, Catanori, Hiramukade, Kyonohimo, Sakuranori, Nikunukade, Tambanori, Tsurutsuru,
Genus Isonohana: Nuracusa, Chronulaxa, Stinging genus, Hirakintoki Genus: Hirakintoki, Matabou: Matabou, Kintoki: Chabokintoki, Kintoki, Matsunori, Komenori, Tosakamatsu, Aedes pine, Kakureto: Genus Otokinari, Genus genus Genus Nagaobane, Genus Funori: Hanafunori, Fukuronori, Makhunori, Karenikusa Genus: Kalexigusa, Tosakamaki Genus: Hosobanotokamaki, Hirohanotosakamodoroki, Yatsudegatotoka, Kurotosakamodoki, Nezashinokidoka, Nezashinokinusakada Genus: Ezotosaka, Tsukasanori: Enashikarimenia, Otsukasanori, Hanagatakarimenia, Honoo: Honoo, Hikagenoito: Hikagenoito, Usuginu, Nikuhono: Nikuhono Oh, the genus Venice: genus Venice, genus Suskabeni: genus Suskebeni, genus Okamuragusa:
Yamadagusa, Mirin genus: Mirin, Hosobamilin, Tosakanori genus: Kikutosaka, Ezonameshi: Ezonameshi, Isomokka: Isomocca, Yukari: Yukari, Hosoyukari, Ibaranori: Ibaranori, Saidaibara, Tachiibara, Kagiinobaliso, Phyllophorus Isanthus, Atsubanori: Atsubanori,
Ogonori: Ogonori, Tsurushiramo, Shiramo, Ogogonori, Mizokonori, Kabanori, Tengusamodoki: Hachijotengusamodoki, Fushikrenori: Fushikenori, Namiiwatake: Namiiwatake, Spiraea:
Sponge, genus: ssp.
Genus: crocodile, saimi, crocodile, crocus genus: genus genus: moss genus, genus cynosum: squid moss, hosoibonori, nobonori, kurohaginnansou genus: kurohaginnansou, akabaginnansou genus: akabaginnansou, genus genus: genus serrata The genus Thaliosa: The genus Thalassium, Hanasakura, The genus Scrophulari: The genus Scrophulari, Schikonori, The genus Hananoeda: Hanaeda, The genus Hirata: The genus Hirata goat, The genus Darus: The darth, Masagoshibari, Anadarus, The genus Webagusa, Benifukunori
Genus Fusinagi: Fussushinagi, Himefusushinagi, Hirohafushinagisagi, Satsumagiso Genus: Hirawaginagiso, Usubawaginagiso, Igis Genus: Igis, Keigisu, Harigigissu, Haneigisu, Amigusa, Egonomida, Chrysanthemum Genus, Genus genus Family: Habutaenori, Konohanori, Suzuronori, Usveni genus: Usbeni, Hasujiginu: Hasujiginu, Nagakonohanori: Nagakonohanori, Suzuginu: Sujiginu, Atubastinu, Hyasubanori: Kagiusubanori, Jaureusubanoli, Jareusubanoli
Genus Ayanishiki: Genus Ayanishiki, Genus Ayaginu: Genus Ayaginu, Genus Dadia: Enashidazia, Genus Shimadagia: Isohagi, Simadadia, Dazimodoki: Genus Dazimomodoki, Genus Itogusa: Moleutogusa, Genus Genus: Makuri, Yanaginori, Hanayana, Rana Salix var. Genus Isomegusa: Isomegusa, Suzinasugusa: Suzinasugusa, Isobashou Genus: Isobashou, Fujimatsumo Genus: Fujimatsumo, Sawtooth Lilium: Scutellaria Rihiba, genus: genus kawamozuku, okamomozuku, himekamozuku, genus idiomyme: ideyugomegome, genus okitamozuku: genus okitimozuku,
Genus genus, genus chinorimo: chinorimo, genus genus: chisinori).

The axle algae (Charus spider, Shiratamamo,
Hossiturimo: Hossiturimo, Lichnotumnus, Flasksmo: Himefuramocha, Chaboflasmo, Tripera, etc., and yellow algae (Hikarimo: Hikarimo etc.).

(14) Materials derived from animal raw materials Chicken extract, bovine / human placenta extract, swine / cattle stomach, duodenum or intestinal extract or its degradation product, swine / cattle spleen extract or Decomposed products, extracts of swine and bovine brain tissues, water-soluble collagen, collagen derivatives such as acylated collagen, collagen hydrolysates, elastin, elastin hydrolysates, water-soluble elastin derivatives, keratins and their decomposed products , Silk protein and its degradation products or derivatives thereof, pig / bovine blood cell protein degradation products (globin peptide), pig / bovine hemoglobin degradation products (hemin, hematin, heme, protohem, heme iron, etc.), milk, casein And its decomposition products or their derivatives, skim milk powder and its decomposition products or their derivatives, lactoferrin or its decomposition products, chicken egg components, fish meat decomposition Such as.

(15) Microbial culture metabolite Yeast metabolite, yeast extract, fermented rice extract, fermented rice bran extract, euglena extract, lactic acid fermented product of raw milk and skim milk powder, trehalose or a derivative thereof.

(16) α-hydroxy acid Glycolic acid, citric acid, malic acid, tartaric acid, lactic acid and the like.

(17) Inorganic pigments Silicic anhydride, magnesium silicate, talc, kaolin,
Bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide,
Titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, red iron oxide, black iron oxide, gunjou, chromium oxide,
Chromium hydroxide, carbon black, calamine, etc.

(18) Ultraviolet absorbing / blocking agent p-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound,
Benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives,
Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives,
Vitamin B6 derivatives, benzophenones, oxybenzone, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, neoheliopan, urocanic acid derivatives, escalol, zinc oxide, talc, kaolin and the like.

(19) Whitening agent p-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound,
Benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives,
Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives,
Vitamin B6 derivatives, oxybenzone, benzophenone, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine and the like.

(20) Tyrosinase activity inhibitor Ascorbic acid and its derivatives, hydroquinone and its glycosides, kojic acid and its derivatives, tocopherol and its derivatives, N-acetyltyrosine and its derivatives,
Glutathione, hydrogen peroxide, zinc peroxide, hydroquinone monobenzyl ether, placental extract, silk extract,
Plant extracts (mulberry, corn, smelt, Clara, mugwort, honeysuckle, yellowfin, dokudami, matsuhodo, adlay barley, edulis, hops, hawthorn, eucalyptus, achillea millefolium, althea, keihi, mankeishi, hamamerisu, yamaguwa, enmeshi, kyoto , Continued spouse,
Sun-dried, mah-ko, senkyu, dokkatsu, psycho, bohu, hamaboufu, ougon, peony skin, peonies, genoshoko, kakkon, licorice, quintoko, aloe, ginger, safflower, green tea, black tea, asenyaku etc.).

(21) Reduction / decomposition of melanin pigment Hydroquinone monobenzyl ether, phenylmercury hexachlorophene, mercuric oxide, mercurous chloride, aqueous hydrogen peroxide, zinc peroxide, etc.

(22) Acceleration of turnover / cell activation Hydroquinone, lactic acid bacteria extract, placenta extract, reishi extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract, fermented milk extract, plant extract ( Aloe, ogon, horsetail, gentian, burdock,
Sicon, carrot, hamamelis, hop, yokunin,
Odikoso, Assemble, Touki, Toukensenka, Amacha, Hypericum, Cucumber, Periwinkle,
Rosemary, parsley, etc.).

(23) Astringent succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcinol, ferric chloride, tannic acid (including catechin compound) Such.

(24) Active oxygen scavenger SOD, catalase, glutathione peroxidase and the like.

(25) Antioxidants ascorbic acid and its salts, stearic acid esters, tocopherol and its ester derivatives, nordihydroguaseletenic acid, butylhydroxytoluene (BHT),
Butylhydroxyanisole (BHA), hydroxytyrosol, parahydroxyanisole, propyl gallate, sesamol, sesamolin, gossypol and the like.

(26) Lipid peroxide production inhibitor β-carotene, plant extract (cultured sesame cells, amateur,
Hypericum, Hamamelis, Clove, Melissa, Emmeiso, Birch, Sage, Rosemary, Minami Minami,
Kina, age, ginkgo, green tea, etc.).

(27) Anti-inflammatory agent Ictamole, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d or dl-
Camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid and its salts, glycyrrhetinic acid and its salts, licorice extract components, sicon extract, age extract extract and the like.

(28) Disinfectant / Disinfectant Acrinol, sulfur, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride, cresol, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or a hydrolyzate thereof, alkyldiaminoethyl chloride Glycine solution, isopropylmethylphenol, triclosan, quinidine, etc.

(29) Humidifier Glycerin, propylene glycol, 1,3-butylene glycol, hyaluronic acid and its salts, polyethylene glycol, chondroitin sulfate and its salts, water-soluble chitin or chitosan derivatives, pyrrolidone carboxylic acid and its salts, lactic acid Sodium, Mini Sasanishiki extract, etc.

(30) Hair preparations Selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine, thianthol, castal tincture, ginger tincture, capsicum tincture, quinine hydrochloride, strong aqueous ammonia, potassium bromate, bromate Sodium, thioglycolic acid, etc.

(31) Antiandrogens Estrogen (estrone, estradiol, ethinylestradiol, etc.), isoflavones, oxendrone, etc.

(32) Peripheral vascular blood flow promoter Vitamin E and its derivatives, plant extracts (such as assembly, garlic, carrot, aloe, gentian, and touki), cepharanthin, carpronium chloride, minoxidil and the like.

(33) Topical stimulants Pepper tincture, nonyl acid vanillamide, cantaris tincture, ginger tincture, peppermint oil, l-menthol, camphor, benzyl nicotinate and the like.

(34) Metabolic activators Photosensitizer 301, hinokitiol, pantothenic acid and its derivatives, allantoin, placental extract, biotin, pentadecanoic acid glyceride and the like.

(35) Antiseborrheic agents Pyridoxine and its derivatives, sulfur, vitamin B6 and the like.

(36) Keratolytic agents Resorcin, salicylic acid, lactic acid and the like.

(37) Oxidizing agent Hydrogen peroxide solution, sodium persulfate, ammonium persulfate, sodium perborate, urea peroxide, sodium percarbonate, sodium tripolyphosphate, sodium bromate, potassium bromate, pyrroline peroxide Sodium citrate, sodium orthophosphate, sodium silicate hydrogen peroxide adduct, sodium sulfate sodium chloride hydrogen peroxide adduct, β-tyrosinase enzyme solution, mushroom extract and the like.

(38) Dye agent 5-aminoorthocresol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 1-
Amino-4-methylaminoanthraquinone, 3,3'-
Iminodiphenol, 2,4-diaminophenoxyethanol hydrochloride, 2,4-diaminophenol hydrochloride, toluene-2,5-diamine hydrochloride, nitroparaphenylenediamine hydrochloride, paraphenylenediamine hydrochloride, N-phenylparaphenylenediamine hydrochloride, meta-hydrochloride Phenylenediamine, orthoaminophenol, N-phenylparaphenylenediamine acetate, 1,4-diaminoanthraquinone, 2,6-diaminopyridine, 1,5-dihydroxynaphthalene, toluene-2,5-diamine, toluene
3,4-diamine, nitroparaphenylenediamine, paraaminophenol, paranitroorthophenylenediamine, paraphenylenediamine, paramethylaminophenol, piclamic acid, sodium piclamate,
N, 'N-bis (4-aminophenyl) -2,5-diamino-1, -4-quinonediimine, 5- (2-hydroxyethylamino) -2-methylphenol, N-phenylparaphenylenediamine, metaamino Phenol, metaphenylenediamine, 5-aminoorthocresol sulfate, 2-amino-5-nitrophenol sulfate, orthoaminophenol sulfate, orthochloroparaphenylenediamine sulfate, 4,4'-diaminodiphenylamine sulfate,
2,4-diaminophenol sulfate, toluene-2 sulfate,
5-diamine, nitroparaphenylenediamine sulfate, paraaminophenol sulfate, paranitroorthophenylenediamine sulfate, paranitrometaphenylenediamine sulfate, paraphenylenediamine sulfate, paramethylaminophenol sulfate, metaaminophenol sulfate, metaphenylenediamine sulfate, catechol , Diphenylamine, α
-Naphthol, hydroquinone, pyrogallol, phloroglucin, gallic acid, resorcin, tannic acid, sodium 2-hydroxy-5-nitro-2 ', 4'-diaminoazobenzozen-5'-sulfonate, hematein and the like.

(39) Flavors Natural animal flavors such as musk, civet, castorium and ambergris, anise essential oil, angelica essential oil, ylang-ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, Guayakwood essential oil, cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, gingergrass essential oil, cedar essential oil, spearmint essential oil, western Mentha essential oil, Great Fengxiang essential oil, Tuberose essential oil, T-shaped essential oil, Orange flower essential oil, Winter green essential oil, Truval sam essential oil, Batturi essential oil, Rose essential oil, Palmarosa essential oil, Cypress essential oil, Hiba essential oil,
Sandalwood essential oil, Petit glen essential oil, Bay essential oil, Vetiva essential oil, Bergamot essential oil, Peruvian balsam essential oil, Boad rose essential oil, Camphor essential oil, Mandarin essential oil, Eucalyptus essential oil, Lime essential oil, Lavender essential oil, Linaloe essential oil, Lemongrass essential oil, Lemon essential oil, Rosemary Vegetable flavors such as essential oils and Japanese peppermint essential oils, and other synthetic flavors.

(40) Dyes / colorants Red cabbage dye, red rice dye, madder dye, anato dye, squid dye, turmeric dye, endu dye, krill dye, persimmon dye, caramel, gold, silver, gardenia dye, corn dye , Onion pigment, tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin, etc.

(41) Others Moisturizing agents, hormones, sequestering agents, pH adjusters,
Chelating agents, preservatives / antibiotics, cooling agents, stabilizers, emulsifiers, animal / vegetable proteins and their degradation products, animal / vegetable polysaccharides and their degradation products, animal / vegetable glycoproteins and their degradation products, Blood flow enhancers, anti-inflammatory agents / anti-allergic agents, cell activators, keratolytic agents, wound treatment agents, foam enhancers, thickeners, oral agents, deodorants / deodorants, bitters, seasonings, enzymes, etc. Various additional and synergistic effects can be expected by using them in combination.

[0070]

Example: Production promoting action of pyrrolidone carboxylic acid (1) Sample γ-PGA (obtained by culturing a strain of Bacillus subtilis in a synthetic medium; average molecular weight = 1,000,000 or more). Plant extract (extract extracted from each plant of aloe vera, chamomile, green tea, arnica, fennel, enmeiso, hypericum, sage, hamamelis, rosemary with a 20 to 50% aqueous ethanol solution).

(2) Test method Prepare test samples prepared by dissolving each sample at each concentration, measure exactly 1.0 mL of each test sample, and monitor the inside of the forearm of a monitor (10 healthy men and women in their 20s and 30s each). After applying to 5cm2 3 times a day in the morning, daytime and night for 3 days, attach a silicon tube to a 4cm diameter funnel in the application area, close the end of the tube with a pinch cock, and degrease it with 10mL of ethyl ether for about 30 seconds. . next,
Using a glass cup with a diameter of 4 cm from the degreased application area,
Extract with 10 mL of purified water for 2 minutes, repeat the same operation twice, combine three times to obtain an extract, and then concentrate this extract by evaporator to dryness, and dissolve in 2 mL of purified water.
The solution is filtered through a 0.45 μm membrane filter to obtain a sample for high performance liquid chromatography (hereinafter, referred to as HPLC). A sample (20 μL) was injected with a microsyringe, and the amount of pyrrolidone carboxylic acid in the skin epidermis was measured three times under the following HPLC measurement conditions. Male and female representatives 2 each
FIG. 1 shows the average results of the names and 10 males and 10 females. Each numerical value in the figure is an average value of three times.

(3) HPLC measurement conditions Column filler: ODS-120A (manufactured by Toyo Soda Co., Ltd.) Column: 4 mm × 250 mm stainless steel Column temperature: 25 ° C. Eluent: 1.0 M sodium sulfate solution (pH 2.2 with phosphoric acid) Eluent flow rate: 1.0ml / min Detector: UV210nm

(4) References for the determination of pyrrolidone carboxylic acid Nakamura, Morikawa et al., "Analytical Chemistry" Vol. 29, pp. 314-318 (198)
0) ・ Koyama, Morikawa, et al., “Japan Cosmetic Engineers Association”, 15 (1), p.45
-53 (1981)-Koyama, Morikawa et al., "Journal of Japan Cosmetic Engineers" 16 (2), P.11
9-124 (1983)

[0074]

Example: Effect of improving skin roughness (1) Sample γ-PGA (obtained by extracting and purifying from natto mucilage, average molecular weight = 300,000). Plant extract (extract extracted with 30-50% ethanol aqueous solution from each plant of Japanese gourd, diatomaceae, honeysuckle, Altea, and Ficus pumila).

(2) Preparation of Sample Specimen 1: γ-PGA (0.1% by weight) + plant extract (solid content: 0.1%)
05% by weight) aqueous solution Sample 2: γ-PGA (0.1% by weight) aqueous solution Sample 3: purified water

(3) Test method Sodium lauryl sulfate (0.2 g) adjusted to 3% by weight with white petrolatum was added to a Hartley-type guinea pig (female, 5 weeks old, 5 animals per group) whose hair was removed from the back for 3 days. Continuous release coating was applied to create rough skin. The part where the skin was roughened was divided into three minutes, each sample (1.0 mL) was applied three times / day, and three days later, the degree of skin roughness was evaluated according to a criterion determined by observation.

(4) Judgment Criteria Erythema and desquamation are scarcely observed 1 point Mild desquamation without erythema 2 points Moderate desquamation without erythema 3 points Desquamation with weak erythema 4 points Moderate erythema Debris associated with 5 points Desquamation with marked erythema 6 points

(5) Test Results The results are as shown in FIG. The external preparation composition of the present invention was recognized to be effective in improving rough skin.

[0079]

[Example] Safety test (1) Primary skin irritation test γ-PGA (Bacillus subtilis strain obtained by culturing in a synthetic medium. Average molecular weight = 1,000,000 or more, 2.0% by weight) and a plant extract (Aloe vera, An aqueous solution prepared by dissolving a mixed extract of 1.0% by weight of 20-50% ethanol aqueous solution from each plant of chamomile, green tea, arnica, fennel, emmeso, hypericum, sage, hamamelis, and rosemary was prepared, and the back was depilated. The Hartley-type guinea pig (five animals per group, weighing about 320 g) was affixed to the skin. The evaluation was performed 24 hours after application using the primary irritancy scoring method with erythema and edema as indices. As a result, in all animals, no erythema and edema were observed, and the animals were determined to be negative.

(2) Cumulative skin irritation test γ-PGA (a strain of Bacillus subtilis obtained by culturing in a synthetic medium, average molecular weight of 1,000,000 or more, 2.0% by weight) and a plant extract (Aloe vera, chamomile, green tea, A Hartley-based solution prepared by dissolving an aqueous solution in which a mixed extract extracted with a 20 to 50% ethanol aqueous solution from each plant of Arnica, Fennel, Emmedium, Hypericum perforatum, Sage, Hamamelis, and Rosemary, 1.0 wt% 0.5 mL / animal was applied to the skin of guinea pigs (female, 5 per group, body weight around 320 g) once a day, 5 times a week. Application was performed over 4 weeks and hair removal was performed on the last application day of each week. The determination was made on the day following the last day of each week by primary irritation scoring, using erythema and edema as indices. As a result, in all animals, 1-4
No erythema and edema were observed over the week, and the test was negative.

[0081]

EXAMPLES Preparation of various external preparation compositions Various external preparation compositions according to the present invention were prepared. The formulation examples are shown below, but the invention is not limited thereto.

(1) Production Example of Lotion A lotion was produced according to the following formulation. % By weight Sorbitol 2 2.1,3-butylene glycol 2 3. Polyethylene glycol 1000 1 4. Polyoxyethylene oleyl ether (25E.O.) 25 Ethanol 10 6. 6. γ-PGA or a salt thereof 0.57. 7. Plant extract (Aloe, Hamamelis, solid content 1.0%) Preservative appropriate amount 9. Purified water 100 residue

(2) Production Example of Emulsion Emulsion was produced according to the following formulation. % By weight Squalane 3 2. Vaseline 1 3. Stearyl alcohol 0.3 4. Sorbitan monostearate 1.5 5. Polyoxyethylene (20) sorbitan monooleate 3 6.1,3-butylene glycol 5 7. γ-PGA or a salt thereof 0.38. Plant extract (chamomile, hops, solid content 1.0%) 2 9. Purified water 100 residue

(3) Production Example of Cream A cream was produced according to the following formulation. % By weight Squalane 20 2. Beeswax 5 3. Refined jojoba oil 5 4. Glycerin monostearate 2 5. Sorbitan monostearate 2 6 Polyoxyethylene (20) sorbitan monostearate 2 7. Glycerin 5 8. 8. γ-PGA or a salt thereof 0.03 Plant extract (Emiso, sage, solid content 1.0%) 3 10. Purified water 100 residue

(4) Production Example of Body Soap A body soap was produced according to the following formulation. % By weight Potassium laurate 15 2. Potassium myristate 5 3. Propylene glycol 5 4. γ-PGA or a salt thereof 15. 5. Plant extract (peach leaves, cornflowers, solid content 1.0%) Appropriate amount of pH adjuster 7. Preservative appropriate amount 8. Purified water 100 residue

(5) Shampoo A shampoo was manufactured according to the following formulation. % By weight Triethanolamine lauryl sulfate 5 2. 3. Polyoxyethylene lauryl ether sulfate Na 12 3.1,3-butylene glycol 4 Lauric acid diethanolamide 2 5. Disodium edetate 0.1 6. γ-PGA or a salt thereof 17. 7. Plant extract (birch, hamamelis, solid content 1.0%) Appropriate amount of fragrance and preservative 9. Purified water 100 residue

(6) Rinse A rinse was produced according to the following formulation. % By weight Stearyl trimethyl ammonium chloride 2 2. Cetostearyl alcohol 2 3. Polyoxyethylene lanolin ether 3 4. Propylene glycol 5 5. γ-PGA or a salt thereof 16. Plant extract (Aloe vera, thyme, solid content 1.0%) 2 7 Appropriate amount of pH adjuster 8. Preservative appropriate amount 9. Purified water 100 residue

(7) Hair Tonic A rinse was produced according to the following formulation. % By weight Ethanol 50 2. Ethyl oleate 1 3. Polyoxyethylene (40) hydrogenated castor oil 24 4. Plant extract (Emophilium, assembly, 1.0% solids) γ-PGA or a salt thereof 0.5 6. Purified water 100 residue

(8) Production Example of Bath Agent (Type A) A bath agent was manufactured according to the following formulation. % By weight Sodium bicarbonate 56 2. 2. anhydrous sodium sulfate 30 Borax 2 4. γ-PGA or a salt thereof 10 5. Extracted plant powder (chamomile, touki) 2

(9) Production Example of Bath Agent (Type B) A bath agent was produced according to the following formulation. % By weight Refined jojoba oil 5 2. 2. Polyoxyethylene sorbitan monolaurate 20 Glycerin monostearate 5 4. Liquid paraffin 2 5. Lauric acid diethanolamide 3 6. γ-PGA or a salt thereof 27. Plant extract (peony, chimney, solid content 1.0%) 10 8. Purified water 100 residue

[0091]

[Examples] Moisturizing action (1) Sample Test product: Emulsion produced in Example Comparative 1: Emulsion produced without adding plant extract Comparative 2: Above produced without adding γ-PGA and plant extract Latex

(2) Test method Epidermis stratum corneum moisture content measuring device (high frequency IMPERDANCE METER mo
del IB 335, manufactured by IBS Co., Ltd.)
The electrical conductivity of the skin was measured with an electrode having an outer diameter of 6 mm and a center diameter of 1 mm. The measurement site is the inside of the forearm flexion side, and the electrical conductivity of the measurement site is measured in advance. Next, a circular filter paper having a diameter of 2 cm containing the sample was attached to the measurement site, the filter paper was peeled off one minute later, and the electrical conductivity was measured every 10 minutes immediately after wiping off the excess sample.

(3) Test conditions Temperature: 22 to 24 ° C. Relative humidity: 50 to 54% Subject: 6 healthy persons Application amount: 1 mL

(4) Test Results The results are shown in FIG. The electric conductivity in FIG.
Represents the average of the names. It was confirmed that the external preparation composition of the present invention can maintain a high moisturizing effect as compared with the comparative product. Furthermore, since a difference is observed in the effect due to the difference in the molecular weight of γ-PGA to be used, a higher effect is required in order to obtain a higher effect.
It is considered preferable to use those having a molecular weight of 000 or more.

[0095]

[Examples] Use test of various external preparation compositions (1) Procedure The lotion, bath agent A, shampoo,
Using the rinse as a test product, a usage test was conducted by male and female panelists (total of 10 persons). In the formulation of each external preparation composition as a comparative product, a preparation excluding the plant extract was prepared, and the test was started 1 to
The test product was used for 3 days, and then the comparative product was used for 4 to 6 days under the following conditions, and the results of the questionnaire were obtained.

(2) Usage conditions Lotion: applied to face immediately after washing in the morning, immediately after bathing at night. 3m each
L, 1 time / day Bath agent A: Bathing bath, 30g, 1 time / day Shampoo: Use during hair washing, 10mL, 1 time / day Rinse: Use immediately after shampoo, 10mL, 1 time / day

(3) Questionnaire Results The results of the questionnaire are shown in Table 1.

[Table 1]

[0098]

The external preparation composition of the present invention, when applied externally, promotes the formation of pyrrolidone carboxylic acid on the skin surface, and prevents skin dryness, rough skin, inflammatory diseases and the like.
It has a reducing or improving effect. In order to increase pyrrolidone carboxylic acid on the skin epidermis by a safe and natural process, the moisturizing effect can be enhanced and maintained without disturbing the quantitative balance and homeostasis of the moisturizing component inherent in the skin. Furthermore, it has an effect of giving elasticity to the skin and preventing rust, and an effect of suppressing creaking on hair and improving hair styling properties.

[Brief description of the drawings]

FIG. 1 shows the application of the external preparation composition of the present invention to human skin,
The result of quantifying pyrrolidone carboxylic acid extracted from the application site after day is shown.

FIG. 2 shows the improvement effect of the external preparation composition of the present invention on guinea pigs having rough skin. The table value is the total value of five animals based on the criteria, and the smaller the value, the higher the improvement effect.

FIG. 3 shows the change in electrical conductivity at the site of application after application of the external preparation composition of the present invention to human skin.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁶ Identification code FI A61K 7/50 A61K 7/50

Claims (1)

[Claims] 1. An external preparation composition comprising γ-polyglutamic acid or a salt thereof and a plant extract in combination.