JP2000178168A - Elastase activity inhibitor and cosmetic composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a new and safe elastase activity inhibitor, and to obtain a cosmetic compisition. SOLUTION: The elastase activity inhibitor and cosmetic composition each comprise one or more kinds of plant oils extracted from the plant group consisting of Magnolia Bark, Schisandra chinensis, Bupleurum chinense, Magnolia liliflora, Angelica acutiliba, Sinomeni caulis et Rhizoma, and forsythia. The inhibitor and composition each provided with an elastase activity inhibitory action can provide skin with gloss and stretchability, prevent the skin from ageing, reduce general wrinkles and fine wrinkles and keep the skin fresh.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to an elastase activity inhibitor containing one or more plant extracts selected from Kokuboku, Domishi, Psycho, Shini, Touki, Bowie, Forsythia, and a pharmaceutical or quasi-drug. The present invention relates to application to various cosmetic compositions in the cosmetics field.

[0002] The field of application is, for example, all kinds of external preparations (including preparations used for animals), and specifically, ampules, capsules, pills, tablets, powders, Granular, solid, liquid, gel or foamed 1) pharmaceuticals, 2) quasi-drugs, 3) topical or systemic skin cosmetics (e.g., lotions, emulsions, creams, ointments, Basic cosmetics such as lotions, oils and packs, facial cleansers and skin cleansers, massage agents, cleansing agents, hair removers, depilatory agents, shaving treatments, after-shave lotions, pre-show lotions, shaving creams, etc.), 4) Medicinal or / and cosmetic preparations applied to the scalp / hair (eg, shampoos, rinses, treatments, permanent liquids, hair dyes, hair dressings, hair tonics, hair growth Such as hair tonic fee), 5) bath agents to invest in use in the bath water, 6) Other, liquid odor deodorant agents and hygiene products, sanitary cotton acids, such as wet tissue, and the like.

[0003]

2. Description of the Related Art A typical change in human skin aging is wrinkles and lumps, which have become one of the major concerns for elderly people and women. However, at present, there are few effective treatments for skin wrinkles and tarmi. At present, various studies on aging are beginning to take place in an aging society.

At present, it is considered that the formation of wrinkles and lumps on human skin is caused by a decrease in the elasticity of the skin, and the factors are related to aging, drying, oxidation, and the effects of sunlight (ultraviolet rays). However, specific histological phenomena include:
Changes in extracellular matrix components such as elastin, collagen, and glycosaminoglycan in the dermis are mentioned, and it is known that changes in elastin are particularly important.

[0005] In other words, it is thought that the decrease in skin elasticity that occurs during the formation of wrinkles and talls involves elastase produced and secreted from dermal fibroblasts being involved in the denaturation of the three-dimensional structure due to elastin damage. It is important to suppress the activity of elastase and to prevent denaturation of elastin, which maintains the elasticity and firmness of the skin, in order to prevent the formation of wrinkles and tarmi, that is, to prevent the aging of the skin.

[0006]

Therefore, the present invention suppresses the activity of elastase, restores and maintains the firmness and elasticity of the skin, thereby preventing the aging of the skin, and provides a youthful skin without wrinkles and lumps. It is an object of the present invention to provide an elastase activity inhibitor (an anti-aging agent) having an effect of maintaining the activity.

[0007]

In view of these circumstances, the present inventors have developed a useful plant having an elastase activity inhibitory effect as a theme of development, and as a result, the present invention has shown that Kouboku, Gomeshi, Psycho, Shiny, Touki, Bowie, Forsythia It was confirmed that at least one plant extract selected from the group significantly suppressed the activity of elastase. And to give the skin gloss and firmness. Alternatively, the present invention completes the present invention by providing a highly safe elastase activity inhibitor and a cosmetic composition which can maintain the skin without wrinkles and skin thinning because it prevents denaturation of elastin which is an elastic fiber. Reached.

[0008]

BEST MODE FOR CARRYING OUT THE INVENTION The term "Koboku: magnolia" used in the present invention means "Magnolaceac" or a plant of the genus Magnolia: Magnolia obovata Thun
berg), Colorado (Magnolia officinalis Rehder et Wil)
sons), Magnolia sprengeri
Pamp.) Or other related plants (Labiatae).

[0009] "Gomishi: Gomiko" is a member of the family Aphididae (Sch
isadraceae), plants of the genus Kadsura juss .: Kadsura japonica L., genus Schisadr
a Michx.) Plant: Datura (Schisadra chines)
is (Turcz.) Baill.) or other dried mature seeds of the same genus plant can be used.

[0010] "Psycho: Saiko" means Umbellife
ra (= Apiaceae)), a plant of the genus Bupleurum L .: Bupleurum scorzoneraefolium Will
d.var.stenophyllum Nakai) or other congener plant roots,
Rhizomes or root bark can be used.

"Shinyi: Shinyi" means Magnolia family (Magno)
laceac), Magnolia L. plant: magnolia (M
agnolia.liliflora Desr.), white magnolia (M.denudata
Desr.) Or other dried flower buds of the same genus plant.

"Touki: Toki" means Umbellife
ra (= Apiaceae)), plants of the genus Angelica (Angelica L.): dried corn (Angelica acutiloba Sieb. et Zucc. Kitagawa.) or other plants of the same genus can be used.

"Bowie: remedy" refers to the family Lacidae (Men
ispermaceae), a plant of the genus Sinomenium Diels: Sinomenium acutum Thumb.Rehd.
et Wils), Plants of the genus Cocculus DC .: Use those obtained from the roots and stems of Cocculus trilobus Thunb. DC. or other congener plants, but other plants of the genus Stephania. L. Hasnokazura
(S.japonica.Thunb.Miers.) Or other roots of the same genus plant,
Those obtained from stems can also be used.

"Forsythia: Forsythia" refers to the Oleaceae family (O
leaceae), Forsythia Vaahl plants: Forsythia suspensa Vahl, Chinese Forsythia (Fo)
rsythia viridissima Lindl.), fruits, pericarp, and those obtained from the spikes, but other species of the same species, such as yamatorenkyo (F. japonica Makino) and kosenrenkyo (F.
koreana (Rehd.)) and the fruit, pericarp, and spikes of F.ovata Nakai can also be used.

The extracts of Koboku, Domishi, Psycho, Shini, Touki, Bowie and Forsythia used in the present invention are various parts of each plant (rhizome, root bark, root, tuber,
Seeds, stems, branches, bark, flowers, flower buds, fruits, pericarp, spikes, etc.) as they are or crushed and then squeezed. Alternatively, it is extracted with a solvent as it is or after pulverization.

Examples of the extraction solvent include water, alcohols (eg, lower alcohols such as methanol, anhydrous ethanol and ethanol, or polyhydric alcohols such as propylene glycol and 1,3-butylene glycol), ketones such as acetone, and diethyl. Esters such as ether, dioxane, acetonitrile and ethyl acetate, and organic solvents such as xylene, benzene and chloroform can be used alone or in any combination of two or more. It can be used even when the objects are combined.

Also, Koboku, garbage, psycho, Shiny,
The plant extracts of touki, bowi, and forsythia may be optionally adjusted by drying, concentrating, or diluting depending on the elastase activity inhibitor to be applied and the dosage form and form of the cosmetic composition.

Although the production method is not particularly limited, it may be usually within the range of the boiling point of the solvent at normal temperature to normal pressure. After extraction, filtration or use of an ion exchange resin is carried out.
A solution, paste, gel, or powder may be obtained by decoloring and purifying. In many cases, it can be used as it is, but if necessary, purification treatment such as deodorization and decolorization may be added as far as the effect is not affected. An activated carbon column or the like may be used, and ordinary means generally applied to the extracted substance may be arbitrarily selected.

Each of the plant extracts of the present invention, i.e., kokuboku, garbage, psycho, shiny, touki, bowi, and forsythia, can be incorporated into elastase activity inhibitors and cosmetic compositions, and the amount thereof is not particularly limited. However, it slightly varies depending on the elastase activity inhibitor, the type and quality of the cosmetic composition, and the expected degree of action, and is usually 0.001% by weight or more (hereinafter, expressed as% by weight), preferably 0.1 to 0.1% by weight.
30% is good. If the amount is less than 0.001%, the effect cannot be expected sufficiently.

In addition, the elastase activity inhibitor and the cosmetic composition of the present invention may be used as pharmaceuticals, quasi-drugs, and cosmetics, if necessary, in addition to the above-mentioned essential components, as long as the effects of the present invention are not impaired. It can be produced by arbitrarily selecting and using components and additives used in the preparation such as the above.

(1) Various fats and oils Avocado oil, almond oil, fennel oil, perilla oil,
Olive oil, orange oil, orange luffer oil, sesame oil,
Cocoa butter, chamomile oil, carrot oil, cucumber oil, tallow fatty acid, kukuinut oil, safflower oil, shea butter oil, soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil , Mink oil, egg yolk oil, palm oil, palm kernel oil, mokuro, coconut oil, tallow, lard, or hydrogenated products of these oils (hardened oil, etc.).

(2) Waxes Beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax,
Montan wax, shellac wax, rice wax, squalene, squalane, pristane, etc.

(3) Mineral oil Liquid paraffin, vaseline, paraffin, ozokeride, ceresin, microcrystalline wax and the like.

(4) Fatty acids Natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, isononanoic acid, caproic acid , 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2
-Synthetic fatty acids such as ethylhexanoic acid and isopentanoic acid.

(5) Alcohols Ethanol, isopyropanol, lauryl alcohol,
Natural alcohols such as cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, and phytosterol; and synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, and 2-octyldodecanol.

(6) Polyhydric alcohols Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol , Polypropylene glycol, 1,3-butylene glycol, glycerin, pentaerythritol, sorbitol, mannitol and the like.

(7) Esters Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyl dodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, Myristyl lactate, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, and the like.

(8) Metal soaps Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate and the like.

(9) Gum, saccharides and water-soluble polymer compounds Arabic gum, benzoin gum, dammar gum, guaiac oil, Irish moss, karaya gum, tragacanth gum,
Carob gum, quinseed, agar, casein, lactose,
Fructose, sucrose and its esters, trehalose and its derivatives, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or hydroxyalkyl (C2-C4) to which alkylene (C2-C4) oxide such as ethylene oxide is added. ) Chitin or chitosan, low molecular weight chitin or chitosan, chitosan salt, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid and its salts, hyaluronic acid and its salts, chondroitin sulfate and its salts, heparin, ethyl cellulose, methyl cellulose, carboxy Methylcellulose, carboxyethylcellulose, sodium carboxyethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, nitrocellulose, crystalline cellulose, Vinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylic acid salts, polyalkylene oxide or crosslinked polymer thereof such as polyethylene oxide or polypropylene oxide, carboxyvinyl polymers, such as polyethyleneimine.

(10) Surfactant Anionic surfactant (alkyl carboxylate, alkyl sulfonate, alkyl sulfate, alkyl phosphate), cationic surfactant (alkyl amine salt, alkyl quaternary ammonium salt) ), Amphoteric surfactant:
Carboxylic acid type amphoteric surfactant (amino type, betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate type amphoteric surfactant, nonionic surfactant (ether type nonionic) Surfactants, ether ester type nonionic surfactants, ester type nonionic surfactants, block polymer type nonionic surfactants, nitrogen-containing nonionic surfactants), other surfactants (natural surfactants) , Derivatives of protein hydrolysates,
Polymer surfactant, surfactant containing titanium and silicon,
Fluorocarbon surfactants).

(11) Various vitamins Vitamin A group: retinol, retinal (vitamin A
1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (vitamin B1), riboflavin (vitamin B2), pyridoxine (vitamin B)
6), cyanocobalamin (vitamin B12), folic acids,
Nicotinic acids, pantothenic acids, biotins, choline,
Inositols, vitamin C group: ascorbic acid and its derivatives, vitamin D group: ergocalciferol (vitamin D2), cholecalciferol (vitamin D
3), dihydrotaxerol, vitamin E group: tocopherol and its derivatives, ubiquinones, vitamin K
Group: phytonadione (vitamin K1), menaquinone (vitamin K2), menadione (vitamin K3), menadiol (vitamin K4), other essential fatty acids (vitamin F), carnitine, ferulic acid, γ-oryzanol, orotic acid, vitamin Ps (Rutin, eriocitrin, hesperidin), vitamin U, etc.

(12) Various amino acids Valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid , Hydroxylysine, arginine, ornithine, histidine, and the like, and their sulfates, phosphates, nitrates, citrates, and amino acid derivatives such as pyrrolidone carboxylic acid.

(13) Various additives derived from plant or animal raw materials These are usually processed according to the type and form of the product to be added (for example, grinding, milling, washing, hydrolysis, Fermentation, purification, squeezing, extraction, fractionation, filtration, drying, pulverization, granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc. You can choose to serve.

The solvent to be used for the extraction may be selected in consideration of the purpose and type of the product to be provided, and the processing to be performed later. However, usually, water and a water-soluble organic solvent (for example, ethanol) are used. , Propylene glycol, 1,3-butylene glycol, etc.).
However, when the content of the organic solvent is not preferable depending on the application, only water may be used, or ethanol which is easily removed after the extraction may be used, and may be used alone or in an arbitrary mixed solution with water. Exploited extract may be used.

In addition, additives derived from plant or animal raw materials are
When applied to systemic or topical external preparations and cosmetics, including protection of skin and hair, moisturizing, improvement of feel and texture,
Gives flexibility, relieves irritation, relieves stress due to aroma, activates cells (prevents cell aging), suppresses inflammation, improves skin and hair quality, prevents and improves rough skin, hair growth, hair growth, hair loss prevention, gloss Application, cleaning effect, alleviation of fatigue, promotion of blood flow,
In addition to cosmetic effects such as a warm bath effect, effects such as flavoring, deodorizing, thickening, preserving, and buffering can be expected.

Specific plants (crude drugs) as raw materials include
For example, almond (Hen peach), eye (Aiba), blue oak
(Seifu Fuji), Aoki (Aoki), Aogiri Kenashiogiri (Otogi), Acacia, Akasho (Akashoma), Akasugi Fruit, Akagrape, Akaminoki (Logwood), Akamegawiwa (Akabu Kashiwa), Akane (Akane grass root),
Jio (Jihuang), Agi (Awei), Akinire (Lauyu skin), Akebi
(Kidori), Asa (Jin Asako), Malva Asagao or Asagao
(Toshiko), hydrangea (hydrangea), amacha (sweet tea), ashitaba (morning leaves), amana (koujimitsu), azuki (red azuki bean),
Acerola, Asenyaku (Asenyaku), Anise, Abemaki fruit, Avocado, Ama, Amacha (sweet tea), Amachaul,
Amadokoro (Tamatake), Amaranthus (Hyuyu, Hageito,
Hymenium spp., Senninkoku, Sugimorigeitou, Hosoaoigaitou, Aogeitou, Haribiyu, Amaranthus hippochondriax), Arigasa lily Bimo (shellfish), Althea, Arnica, Alpinia katsumadai (Souzuk), Aloe, Rokae, aloe vera, aloe vera
Anzu Honganzu (Anjin), Ansokkou (Benzo), Ikakozolina (Biliary Head), Epimedium Yachimataikarisou (Inyokaku), Igusa (Wicked Grass), Knotweed (Tiger root), Yew (1st), Strawberry, Fig ( Figurine or its leaves, Ichihatsu (primary), Ichibi (Aoi-ko), Ichiyakusou (Ichiyaku herb), Ginkgo (Ginkgo leaves, Ginkgo), Itohimemegi (Enshi), Carob, Inu-zuna (Tailexi),
Inubeyu (Honagainubiyu), rice seed or seed coat is seed coat, Inondo seed, Ibuki-kosou, nettle, ylang-ylang, iwa-tobacco (Iwatochichi), iwahiba-iwawamatsu (makikashiki), oolong tea, wikyo (fume), ukiyagara (mishaki), Uguisukagura fruit, Himeuikyo, Turmeric (Utsukin), Duckweed (Kyokan), Usubasaishin / Keirinsaishin / Aushousaishin (Spicy), Usvenia oy, Usvenitachiaoi, Utsubogusa (summer hay), Udo or Shishido (Qui-yi) Independent enthusiasts, Japanese ume, or their pulp, Vulgaris occidentalis, Uwaurushi (Kusaco peach), Unshurikan (Chen skin), Estragon, Ezocogi (Ezo Goka), Echinashi (Hosobamurasakibarengiku), Echinida, Enokitake, Enotake Ebisugusa / Cassia
Tola (Keiko), Elderberry fruit, Elemi, Elingy (Pre-Lotus Elinge), Endu (Gossip, Gossip rice), Ogi-kibana-ohgi (Ohgi), Ouren (Yoren),
Giant crows (caronin), giant guruma (civil engineering incense), giant psyllium (car hors, car hors grass), toucan hazel (Haruko), giant horned okera (white aphid), fruit of hayabaya shabushi, eucalyptus hawthorn (Yamakoko), eusay (narco lily,
Kagi Kurumabanarukoyuri), Sasanoyuki, Sasanoyuki, Barley (barley), Ochosigusa (Grain sperm), Okazeri (Snake bed), Oguma (Wrapping), Okra fruit, Panax ginseng and Caribbean (Carrot), Brassica napus St. John's wort (brother's cut grass), St. John's wort (intermittent), Onomimomi (blue ears), Onigurumi, Onidokoro Tokoro Nagadokoro (Hikai), Oninoyagara
(Tianhe), Onyuri Sasayuri Hakatayuri (Yuri), Ononis, Halibut (Hihayu), Ominaeshi (Loose), Dutch mustard (Cresson), Dutch jelly, Dutch honey, Olive (fruit, seed, leaf), oregano , Orange fruit or pericarp, lycaenium (ripe yellow), cacao fruit, pericarp,
Seeds, oysters or their leaves (persimmons), Kagikazura (crop hooks), persimmons (canthus), cassia, kasinaki fruits (peony), gajutsu (shabatsutsutsu), kashiwa (pajuju), Cascarilla, Cascara sagrada, Kanixa (Kinosato), Valerian (Kisosane), Birch or Weeping birch (White birch), Pumpkin, Capokinoki seeds, Kahokokusansho (shopper), Gama (Kamao), Chamomile, Roman chamomile, Camillade Weeds), Kamkamu / Kamo-duck, Calla-uri / Sinara-ka-souri (Our gourd), Rasabi-shaku (Midsummer), Raspberry, Guarana seed, Karaya, Karin (Gourd), Garcinia, Kawamidori, Kawara Psycho , Kawaratake, Kawaranadeshiko (Ishitake), Ezokawaranadeshiko (Qumugi, Quumiko), Caroline carrot (Qingkoo), Kawarayogi (Inchinko), Kansui (Kansu), Japanese elephant (licorice), cantaraasa, candelilla, cantola, canna, raspberry (ezo strawberry, dutch strawberry, shrimp strawberry, nawashiro strawberry, maple strawberry, european strawberry), kiwi fruit, kikarasuuri (uryu root), kikyo (bellflower, kikyo root) (Chrysanthemum flower, olive citrus, artichoke), catalpa (Azumitsu), daisies (yotei), pheasant (komitsu), kizuta, kidachi aloe, kidachi mint, kina, chinoki (shinkona, akakinoki), yellowfin (yellowwood),
Gymnema sylvesta, cabbage, immature cabbage, cucumber, gyoryu (Nishikawayanagi / Teriyanagi), Kiraja saponaria, Kiraya, Kylansou (acne rush), kumquat fruit, betel, kinmizukiki (sengan grass), guava fruit, guayule, Quercus Infectoria (Garlic), Kukui Nuts, Kukui (Gugi, Gyugia, Gyugia Leaves, Skeletal Bark), Kusagi Kazura (Tenmen Fuyu), Kuzu (Kakkon), Camphor Tree, Gooseberry Fruit, Xining Ginseng, Gardenia (Gardenia), Kunugi (Bokusou), Kumazasa, Kumatsuzura (Echispera), Clara (Bitterfruit), Cranberry fruit, chestnut or its astringent skin, Curculigo latifolia fruit, Grapefruit, Buckthorn, Black squirrel, Rescue Cloves (cloves, cloves), Gumbinazuna (sekimei, sekimeishi), keiga B
Celosia (cow flower, rooster), bay laurel, kenashisarutoribara (Tsubukuryo, Yamakiko), gentian,
Gennoshoko (Old treasured grass), Kemponashi (Kigushi), Koukisekikoku, Kishikukan / Koji / Tachibana / Ovenimican / Fukulemican / Sagamikouji / Ponkan / Santara (Tachibana bark), Koshinbara (Monthly flower), Kosuihakka, Kozo fruit, Kozocha (Tea), kouhone (river bone), kouhon (straw and karawara), kouriang, kourikyo
(Kogaro), Cilantro fruit, Kourouen (Kohren), Koganebana (Yellow Gon), Kokemomo (Koshibachi), Coconut fruit, Goshuyu (Gusyuyu), Goshostrawberry (Ubonashi), Pepper (pepper), Copaiba balsam, Coffee beans , Kobushi, Burdock
(Burdock, burdock), crocodile, sesame (sesame), sesame clover, stalk beetle, vine, wheat, wheat, rice, rice bran (red bran, white bran), rice oil, cola.
Acuminata seed, Cola vera seed, Fenugreek fruit, Colombo, Kondulango, Kombu, Konjac, Comfrey
(Final-grown grass), sisal (sisalasa), saxima button-pear cinnamon-powder shinsensensou (weird sensin), cherry tree (oshima-zakura, yamazakura, oyamazakura),
Leaf, flower, fruit, bark (cherry bark), cherry, pomegranate, sasa, sasanqua, sajiomodachi, sweet potato, sweet potato , Sugar beet, jujube jujube (acid jujube), saffron (red safflower, western safflower), pomelo fruit, savonso, salmonberry fruit, sarasinosa (mamasa), salvia (sage), sawagikyo (sangansai), sawagurumi (sangan peach) ), Panax ginseng, Panax ginseng (Sax carrot), Sanshuyu (sanshuyu), Sansho (Sansho), Sandskon (Yamazone), Shea
(Carite), cyanoki fruit, shiitake mushroom (shiitake mushroom), shion (shion), dikitaris, shikunishi (mikomiko), perilla, aodiso, chirimendiso, katamendiso (shiso leaf, shiso), rosewood, linden, cinnamonoki, cinnamonium ),
Shimeji (Hinshimeji, Shakashimeji, Hatakeshimeji, Oshiroishishimeji, Bunashimeji, Shirotamogitake), Shimotsukesou, potato, Peony (Shakuyaku), Shajin (Shasan), Janohige (Motomonto), Shukushamitsu (Sandin, Sand sand), Juzuma , Palm fruit, ginger (ginger), ginseng hydrangea (Tsuneyama), shobu (iris, iris root), shizuku fruit, shirakashi seed, shirogoshi butterfly seed, white azuki bean (chicken bone grass), white iris (small iris), white blossom , Shirominanten (Nantami Minami), Cinchonasakusylbra, Jingju (Mizuka, Mizuka, Shencho), Cinnamon, Watermelon (Watermelon), Honeysuckle (Golden Flower, Ninwinter), Watermelon (Omigami), Swimberry Fruit, Stevia, Strawberry Fruit, Suzu Psycho (Lord Xu Chang), Horsetail
(Thorn), Purslane (Horse Tongue, Hen Tonen), Plum Fruit, Scots Pine Cones, Larix sylvestris, Scots Kizuta, Scotch Worm, Scots Pear, Scots Dandelion, Horse Chestnut (Maroniye), Scots Pear Atlantic snowfall, Atlantic elderberry (elder), Japanese shrew (Juniper / Morimatsu), Yarrow (milfoil), Yeoubara, Yeouenbokuboku, Yeodori dorigi, Yeohwakae, Yeahwase, Oysters Mushroom, mallow, hirohasenega, senega, seri, celery, senkyu (river cucumber), senshinren (perforated reed), sendan, senburi (drug), senna fruit or leaves, senninso (great tate), souka (grass) ),
Buckwheat seed, soybean potato, rhubarb (daisou), radish, soybean, daidai (orange peel, kikomi), takasaburo (dry lotus),
Takatodai (Daigei), Takawarabi (Inu), Dark Sweet Cherry Fruit, Tachiaoi, Tachijakosou (Thyme, Hyakurika), Tachidokoro (Hikai), Tachiyanagi, Tamarind Seeds, Onion, Taranoki or its root bark, Tanjin (tansan), Dandelion (Kaba Koei) or White dandelion
Moko dandelion, dammar, cherry fruit, chiyagaya or its root (chinese), chikusetsu carrot (bamboo ginseng), chicory,
Datura (Rhuanghuang), Korean ginseng (Wuyi), Korean ginseng (Ginseng), Artemisia mosaic (Aiba), Choreimaitake (Irei), Tsukimiso, Tsukutake mushroom (Mushroom), Camellia, Camellia, Tsumekusa (Lacquer grass) ,
Twigweed (duckling grass), vine adzuki (red azuki bean), tsuldokudami
(Hanshu crow), Crucian carp (Ban apricot), Crucian carrot (Four leaf ginseng), Tsubaki, Deiko, Teuchigurumi, Duberry fruit, Tengusa, Tencha (Tencha), Tendaiyaku (Warner), Touga
(Winter gourd), lizard sparrow, capsicum (pepper), calendula (marigold), tunavena (river cut), corn or corn hair (southern hair), horn rat mochi
(Female Sadako)
Tokusa (pirate), Dokudami (jujutsu), Tokon (root), Toshishi, Mamedaoshi and Neashikazura, Eucommia (Tonaka, Tonakaha), Ash (Cowhide), Tomato, Tragant, Triacanthus seed, Tormentilla, Doronoki, Troroaoi, Nai Zeriaberry fruit, yam, yam (Yamayaku), squid (butcher bloom), naginata kouju, nazuna, rapeseed, natame beetle (sword bean), natsumikan, jujube (daijutsu), nanawaibara (gold cherry), navena (continued) ), Nameko, narco lily (yellow), peanut
(Peanuts), Nanten (Minami Tenmi), Namban Karamushi (ramie), Nigaki (Maki), Nigamogi (Gumen), Nikuzuku, Kay Nikkei Ceylon Nikkei Yabnikkei (Caesar)
・ Fruits of Keishi (Keishi), Chinese chive (Nirako), elderberry (bone-attached tree), flower or foliage, garlic (garlic), nulde (fivefold child), leek, nemoki, neb, nebi, nemlinoki, jigokubana
(Healing), Nomi Thistle (Daichi), Noibara (Fruit), Yarrow, Nodake (Mae-hu), Nobara, Nomomo, Palm, Pineapple Fruit, Hibiscus (Bussouge, Furin-Bussouge, Roselle), Hakamauraboshi (Bone Crushing), Hakusen
(Ringworm skin), huckleberry fruit, chickweed (red), hazel
(Haruko), Hasiridoro (Roth root), Basil, Lotus (Lotus,
Lotus meat, Renko, Parsley (Netherland jelly), Naked mussels, Batata, Hachik madake (Bamboo boiled), Patchouli, Mint (Light load, Light load leaves), Barley (Yokinin), Hanasuge (Chimo), Banana, Hanahakka, Hanabishi (Siturishi, Shishitsuri), Vanilla bins, Papaya, Scotch grass (Rabbit malt grass), Habu grass, Paprika, Hamagou, Mitsubahamago (Thunboshi), Hamasuge (Kazukiko), Hamabishi (Situriko), Hamanas (Maikai flower), Hamabofu (beach storm), hamamelis, rose (rose), agaric (aratake, white-bellied agaric, uskimorinocasa), haran, parietaria, harunire (yellow, yu-white skin, yuba), handaikai (gyutai),
Breadfruit, Hiougi (sun-dried), Lycopodium carrot (party party), pecan nut, giganbana (stone garlic, mulberry flower),
Hikiokoshi (prolonged life grass), Hishi (Hishimi), pistachio, beet, Atsuba (Ishire), Atsuba Echinida, Hinata Kozuchi (cow knee), Hinoki, Hiba, Castor, Sunflower, Bell pepper, Himeuzu (Hollyhock), Himegama ( Kabama), Himematsutake (Kawariharatake, Hiromatsutake), Pimenta fruit, Juniper, Bjakuyu, Hydrangea fruit, Beloyd mallow, Hiroha okinagusa (Shiratooka), Loquat (Loquat, Loquat leaf), Betel wax (Large garment, Betel nut), Southern rattan), butterbur, butterflies (winter flowers, winter leaves), fujibakama (orchid grass), wisteria beans (flat beans), grape fruit or pericarp, seeds and leaves, beech, beetle, bamboo shoots, Brazilian liquorice, Brazil carrot, Black currant fruit, blackberry, plum fruit, flucellaria, blueberry (Prunus avium), prunes, broth De psyllium, Bundou (mung beans), loofah, safflower (safflower), Heneken, belladonna, berry fruit, Pell Lesbian Berg, pinnata, Ikikusa (Keiten), boysenberry fruit, kochia,
Niwaxa, Nendo, Hahagigi, Kokia (Dermatology), Balsam (Hossen, Acute, Boneless), Bofu (Windbreak), Spinach, Physalis (Torone), Bokeh (Gourd), Hosobanaokera (Sojutsu), Hosobano Giraffe (Sancheong Jingtian), Hosobabarengiku, Bodaiju (Bodai tree), Button (peony, peony skin), Hop, Jojoba, Whiteberry berry fruit, Hongonik (Nikujiryou / Daigei), Hongshimeji, Hongsekoku
(Steel Stone Stone, Sparrow Mountain Stone Mouse), Meisulten Nanshaw (Tennan Star), Maitake (Maitake), Ephedra (Maoh), Macadamia Nuts, Macri (Seaweed), Magwa (Mulberry White Skin, Mulberry), Magnolia Splengeri, Matatabi (Kinten Tate), Matsukasa, Matsuba, Matsuhodo (Bukuryo), Mayorum (Hanamint), Malvanozadine (Bittersweet), Mulberry Fruit, Quince, Mango, Mangosteen, Mansugurumi, Mandarin Fruit, Mannentake (Reishi), Fruits of Citrus genus (Kikomi), Psyllium mizuasagao (Ryugoshi), Mizokukushi (Hanabe lotus), Misohagi (Chikuna), Mizunagi / Niwayanagi (Kenki), Mitsugashiwa, Mitsuba, Midorihakka, Mimosa, Myoga, Miracle Fruit fruit, Myrrh, Myrobaran, Mukuge (Mokuge), Mukunoki, Mokuroji, Murasaki
(Purple root), purple squirrel, purple pearl, purple corn, purple squirrel (Kunming chicken blood wisteria), red sea squirrel (mother grass), red seaweed, melaleuca,
Melissa, melilot, melon fruit, sagebrush, moso bamboo, mokkou (wood incense), maple blossom, peach leaf (peach leaf), fruit and seed (peach), bean sprouts, morello cherry fruit, moroheiya (yellow), yakawamurasakiimo , Yakuchi
(Masutoshi), cornflower (cornflower), cornflower mint, yashabushi (arrow car) / Himeyashabushi / Obayashishabushi fruit, peel, fruit ear, yachiyanagi, yatsude (octagonal lantern), mistletoe (willow parasite), willow (kawayanagi, tayanagi, Weeping willow, red willow willow, pussy willow, inukoriyanagi, kinuyanagi, kolyanagi, unryuyanagi, miyayamanagi, yamayanagi, shirabanyanagi, tyrikkinuyanagi, foxeye willow, drone, purple tobacco, golden tobacco ), Pokeweed (land), alder
(Yamaharu), Bayberry (Plum skin), Bayberry, Eucalyptus,
Yukinoshita (Tiger ear grass), Yucca flevifolia, Yuzu fruit, Lily, Yologusa, Artemisia (Aiba), Lygan (Rainuma), Lime fruit, Rye, Ranka fruit, Raspberry leaf / fruit, Ratania (Clameria), Rakkyo Eshaloto (Scallop), lavender, longan (longan flesh), agave (Aon agave, fukurin agave), ryocha (green tea), apple fruit, gentian, rubus sabisimsims (tenryu), litchi (lichi, lichi nucleus), lettuce (chisha) , Red currant fruit, lemon fruit, lemongrass, astragalus, wax palm, locon (reed: Root), loganberry fruit, rosemary
(Mannenrou), Rosehip (Nobara), Wasabi and Waremokou (Jiyu).

In addition, other seawaters, for example, seawater salt, dried seawater, inorganic salts obtained from the Dead Sea or the Atlantic or Pacific Ocean (sodium chloride, magnesium chloride, potassium chloride, etc.), sea mud / mud (fango) Sea mud or mud (silicon dioxide, silicon dioxide, etc.) such as Italian fango, German fango, Eifelfango, Freiburgfango, etc.
Titanium dioxide, aluminum oxide, iron oxide, manganese oxide, sodium oxide, potassium oxide, magnesium oxide, calcium oxide, strontium oxide, sodium, potassium, magnesium, calcium, chromium,
Iron, copper, nickel, zinc, lead, manganese, arsenic, water, vitamin B, vitamin A, phytohormone "diberlin,
Cytokinin, absciscin ")

Examples of the seaweeds include seaweeds [green algae: Chlorella vulgaris, Chlorella pyrenoidsa, Chlorella ellipsoidia, Aonori (Usbaaonori, Susiaonori, Hiraaonori, Bowaoonori, Hosoedaoonori)], and seaweed [brown alga: Kombu algae: brown algae: brown seaweed: , Resiliconibu, hosomekobu, mishitishibu), seaweed, hirome, blue seaweed, giant kelp (macrocystis pilifera, macrocystis integrifolia, neocitys luetokeana), hijiki, hibamata], seaweed [red algae: hijirimen, maxa ( Tengsa),
Hirakusa, Onyxa, Obakusa, Kataobaxa, Yatabegusa, Yuikiri, Shimatengusa, Tosakanori, Togekirinsai, Akumasakirinsai, Kirinsai, Byaksinkirinsai, Tsunomata, Obatsunomata, Tochaka, Tohkanota, Tojika , Ibotsunomata, Yaleus Banori, Kagius Banori,
Suzius Banori, Hyus Banori, Akamomininori] and the like.

Other algae such as green algae (Chlamydomonas spp .: Chlamydomonas sp., Akayukimo, Dunaliella spp .: Dunaliella spp., Chlorococcus spp .: Chlorococcus, Kwanomimo spp .: Kwanomimo spp., Volvox spp. Spirogyra: Hizaori, Spirogyra, Tulsimidro, Hibimidro: Hibimidoro, Aosa: Anaoasa, Amiosa, Nagaosa, Kawanori: Kawanori,
Genus Fricciella, genus genus: medusa, asamidori ogusa, kawashiogusa, marimo, valonia: tamagobaronia, tamabaronia, magnum: tamatamamo, iwata: husaiwata, suricogita, heraiwirata, crocodile, crocus Sakibutimimil, Nagamil, Hiramil, and Casanori:
Casa nori, Juzumo
Mizojuzumo, Mizuazumimo, Korekaete, Tsutsumimo, Kikkogusa: Kikkogusa, Hitoexa: Hitoexa, Hirohanohiegusa, Usuhitoexa, Motsuhitoe, Sayamidro, Genus spp.

Blue-green algae (Genus L .: L., L., L., L .: L., mushrooms,
(Hassai, Yuremo, Racenmo (Spirulina) genus: Spirulina, Trichodesmium (Aiacacio), etc.).

Brown algae (Piraella spp .: Piraella spp., Shiomidoro spp .: Namiami sp., Isogrape spp .: Isogrape,
Genus Isogawara, genus Isogasula, genus genus: Gunsenkurogashira, genus Kashirazaki, genus Kashirazaki, genus Muchimo: genus Muchimo, Hiramutimo, Kebelligsa, genus Amis. Artemisia spp.
Genus Matsumo: genus Matsumo, genus matsumo: genus sylvestris, genus okinawa, genus okinawa-mozuku, genus niseftomozuku: niseftomozuku, futomozuku: genus futomozuku, ishimozuku: ishimozuku, kuromo: kuromo, nisemozuku: genus, spruce, spruce Ishige, Irolo, Icmegasa: Ichimegasa, Keyari: Keyari, Umibos: Umibos, Urushigusa: Urushigusa,
Species of the genus Coleoptera, Tobacco planta, Laminaria genus: Laminaria genus: Habanomodo genus: Habanomodo genus, Habanori genus: Havanori, genus Habanori: genus Habanori, genus bukuro: common bukuro, Ezobukuro genus: Ezobukuro, Fukuronori genus: Fukuronori, Watanabe Genus Kagomenori: Kagomenori, Muraridori: Murachidori, Shakusugusa: Sharks,
Genus Genus: Genus Genus, Genus Genus: Genus Genus: Genus Genus, Genus Kayamonori, Genus Kayamono, Genus Genus: Genus Ligustrum, Genus Tsurumo: Genus Turrum, Genus Anana: Genus Anastasia, Genus Ligustrum: Sujime, Genus Missiscoma: Genus Genus Ligustrum, G. , Onikombu, Goheikombu, Nagakombu, Pea kelp, Ochimikombu, Torokombu genus: Torokombu, Antokume genus: Antokume, Kajime: Kajime, Tsuruarame, Kurome, Kikuishikomu: Genus Kikuishi, Nejirekomu: Neshilecomb, Kuroshiomeko : Nekoshikambu,
Alame: Alame, Ainuwakame: Ainuwakame, Chigaiso, Oniwakameezoishige: Ezoishige, Yabanemoku: Yabanemoku, Rapamoku: Rapamoku, Jiromoku: Uganomok, Jiromoku, Hiemomoku, Honamomaku, Tamamomaku, Tamamomoku Akamoku, Shidamoku, Hondawara, Nejimoku, Narasamo, Mametawara, Tatsukuri, Yasumatamoku, Umitranoo, Oobamoku, Fushizushimoku, Hahakimokum, Togemoku, Jolemoku, Saw-mokum, Obanoki-moku, Genus genus: Genus
Bulukimo, genus cynomolgus: cynomolgus etc.).

Red algae (Genus genus: Ushikenori, Funorinoushike, Genus Amanori: Asakusanori, Susabinori,
Genus Upirinori, Onimanori, Tasa, Filitasa, Benitasa, Rhodocorton: Milnoveni, Akebonomozuku: Akebonomozuku, Konahada: Haikonahada, Yogorekonahada, Aokonahada, Sea Species: Acer genus : Larch, Fusanori: Fusanori, Nisefusanori: Nisefusanori,
Sodegarami: sodegarami, rattles: rattle, hiraragara, hirohatamataki: hirohatamatamadaki, tamamaidaki: tamaitadaki, kakenokenori: kaginori, kakenenori, tengusa, kurobana, kurobana, swordfish, swordfish, squirrel Genus: hibiroudo, himehibiroudo,
Genus genus: genus isensis, genus genus:
Genus spruce, Ryumonsou: Ryumonsou, Hera Ryumon, Nisekarequigusa: Nisekarequigusa, Okitsubara genus: Obaokitsubara, Akaba: Akaba, Malvaacaba, Naminohana: Genus Hosobanamino, Naminohanagosa, Coralthus Genus Ikawanokawa, Genus Kanokawa: Kinokawa, Genus Kaninote: Kaninote, Coralum: Coralum, Genus Mucadenori: Mucadenori, Sujimukade, Catanori, Hiramukade, Kyonohimo, Sakuranori, Nikunukade, Tambanori, Tsurutsuru,
Genus Isonohana: Nuracusa, Chronulaxa, Stinging genus, Hirakintoki Genus: Hirakintoki, Matabou: Matabou, Kintoki: Chabokintoki, Kintoki, Matsunori, Komenori, Tosakamatsu, Aedes pine, Kakureto: Genus Otokinari, Genus genus Genus Nagaobane, Genus Funori: Hanafunori, Fukuronori, Makhunori, Karenikusa Genus: Kalexigusa, Tosakamaki Genus: Hosobanotokamaki, Hirohanotosakamodoroki, Yatsudegatotoka, Kurotosakamodoki, Nezashinokidoka, Nezashinokinusakada Genus: Ezotosaka, Tsukasanori: Enashikarimenia, Otsukasanori, Hanagatakarimenia, Honoo: Honoo, Hikagenoito: Hikagenoito, Usuginu, Nikuhono: Nikuhono Oh, the genus Venice: genus Venice, genus Suskabeni: genus Suskebeni, genus Okamuragusa:
Yamadagusa, Mirin genus: Mirin, Hosobamilin, Tosakanori genus: Kikutosaka, Ezonameshi: Ezonameshi, Isomokka: Isomocca, Yukari: Yukari, Hosoyukari, Ibaranori: Ibaranori, Saidaibara, Tachiibara, Kagiinobaliso, Phyllophorus Isanthus, Atsubanori: Atsubanori,
Ogonori: Ogonori, Tsurushiramo, Shiramo, Ogogonori, Mizokonori, Kabanori, Tengusamodoki: Hachijotengusamodoki, Fushikrenori: Fushikenori, Namiiwatake: Namiiwatake, Spiraea:
Sponge, genus: ssp.
Genus: crocodile, saimi, crocodile, crocus genus: genus genus: moss genus, genus cynosum: squid moss, hosoibonori, nobonori, kurohaginnansou genus: kurohaginnansou, akabaginnansou genus: akabaginnansou, genus genus: genus serrata The genus Thaliosa: The genus Thalassium, Hanasakura, The genus Scrophulari: The genus Scrophulari, Schikonori, The genus Hananoeda: Hanaeda, The genus Hirata: The genus Hirata goat, The genus Darus: The darth, Masagoshibari, Anadarus, The genus Webagusa, Benifukunori
Genus Fusinagi: Fussushinagi, Himefusushinagi, Hirohafushinagisagi, Satsumagiso Genus: Hirawaginagiso, Usubawaginagiso, Igis Genus: Igis, Keigisu, Harigigissu, Haneigisu, Amigusa, Egonomida, Chrysanthemum Genus, Genus genus Family: Habutaenori, Konohanori, Suzuronori, Usveni genus: Usbeni, Hasujiginu: Hasujiginu, Nagakonohanori: Nagakonohanori, Suzuginu: Sujiginu, Atubastinu, Hyasubanori: Kagiusubanori, Jaureusubanoli, Jareusubanoli
Genus Ayanishiki: Genus Ayanishiki, Genus Ayaginu: Genus Ayaginu, Genus Dadia: Enashidazia, Genus Shimadagia: Isohagi, Simadadia, Dazimodoki: Genus Dazimomodoki, Genus Itogusa: Moleutogusa, Genus Genus: Makuri, Yanaginori, Hanayana, Rana Salix var. Genus Isomegusa: Isomegusa, Suzinasugusa: Suzinasugusa, Isobashou Genus: Isobashou, Fujimatsumo Genus: Fujimatsumo, Sawtooth Lilium: Scutellaria Rihiba, genus: genus kawamozuku, okamomozuku, himekamozuku, genus idiomyme: ideyugomegome, genus okitamozuku: genus okitimozuku,
Genus genus, genus chinorimo: chinorimo, genus genus: chisinori).

The axle algae (Characta spp., Rhododendron spp.,
Hossiturimo: Hossiturimo, Lichnotumnus, Flassica: Himefuramo, Chaboflasmo, Tripella, etc., Yellow algae (Hikarimo: Hikarimo etc.) and the like.

Materials derived from animal raw materials include a cockscomb extract, a cow / human placenta extract, a pig / cattle stomach, duodenum or intestinal extract or a decomposition product thereof, and a pig / cattle spleen extract. Products or their degradation products, extracts of swine or bovine brain tissue, water-soluble collagen, collagen derivatives such as acylated collagen, collagen hydrolysates, elastin, elastin hydrolysates, water-soluble elastin derivatives, keratin and its decomposition products Or a derivative thereof, a silk protein and its degradation product or a derivative thereof, a pig / bovine blood cell protein degradation product (globin peptide), a pig / bovine hemoglobin degradation product (hemin, hematin, heme, protohem, heme iron, etc.), milk , Casein and its decomposition products or their derivatives, skim milk powder and its decomposition products or their derivatives, lactoferrin or its decomposition products, chicken egg ingredients Such as fish meat decomposition products.

(14) Microbial culture metabolites Yeast metabolites, yeast extract, rice ferment extract, rice bran ferment extract, euglena extract, lactic acid fermentation of raw milk and skim milk powder, trehalose or derivatives thereof.

(15) α-hydroxy acid Glycolic acid, citric acid, malic acid, tartaric acid, lactic acid and the like.

(16) Inorganic pigments Silicic anhydride, magnesium silicate, talc, kaolin,
Bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide,
Titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, red iron oxide, black iron oxide, gunjou, chromium oxide,
Chromium hydroxide, carbon black, calamine, etc.

(17) UV absorbing / blocking agent p-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound,
Benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives,
Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives,
Vitamin B6 derivatives, benzophenones, oxybenzone, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, neoheliopan, urocanic acid derivatives, escalol, zinc oxide, talc, kaolin and the like.

(18) Whitening agent p-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound,
Benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives,
Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives,
Vitamin B6 derivatives, oxybenzone, benzophenone, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine and the like.

(19) Tyrosinase activity inhibitor Ascorbic acid and its derivatives, hydroquinone and its glycosides, kojic acid and its derivatives, tocopherol and its derivatives, N-acetyltyrosine and its derivatives,
Glutathione, hydrogen peroxide, zinc peroxide, hydroquinone monobenzyl ether, placental extract, silk extract,
Plant extracts (mulberry, corn, smelt, Clara, mugwort, honeysuckle, yellowfin, dokudami, matsuhodo, adlay barley, edulis, hops, hawthorn, eucalyptus, achillea millefolium, althea, keihi, mankeishi, hamamerisu, yamaguwa, enmeshi, kyoto , Continued spouse,
Sun-dried, mao, sengoku, dokkatsu, psycho, boufu, hamabofu, ougon, peony skin, peonies, gennoshoko, kakkon, licorice, quintoko, aloe, ginger, safflower, green tea, black tea, asenyaku, etc.).

(20) Melanin dye reduction / decomposition Hydroquinone monobenzyl ether, phenylmercury hexachlorophene, mercuric oxide, mercurous chloride, aqueous hydrogen peroxide, zinc peroxide and the like.

(21) Acceleration of turnover / cell activation Hydroquinone, lactic acid bacteria extract, placenta extract, reishi extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract, fermented milk extract, plant extract ( Aloe, ogon, horsetail, gentian, burdock,
Sicon, carrot, hamamelis, hop, yokunin,
Odikoso, Assemble, Touki, Toukensenka, Amacha, Hypericum, Cucumber, Periwinkle,
Rosemary, parsley, etc.).

(22) Astringent Succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcinol, ferric chloride, tannic acid (including catechin compound) Such.

(23) Active oxygen scavenger SOD, catalase, glutathione peroxidase and the like.

(24) Antioxidants ascorbic acid and its salts, stearic acid esters, tocopherol and its ester derivatives, nordihydroguaseletenic acid, butylhydroxytoluene (BHT),
Butylhydroxyanisole (BHA), hydroxytyrosol, parahydroxyanisole, propyl gallate, sesamol, sesamolin, gossypol and the like.

(25) Lipid peroxide production inhibitor β-carotene, plant extract (cultured sesame cells, amateur,
Hypericum, Hamamelis, Clove, Melissa, Emmeiso, Birch, Sage, Rosemary, Minami Minami,
Kina, age, ginkgo, green tea, etc.).

(26) Anti-inflammatory agent Ictamole, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d or dl-
Camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid and its salts, glycyrrhetinic acid and its salts, licorice extract components, sicon extract, age extract extract and the like.

(27) Antibacterial, bactericidal and disinfecting agents acrinol, sulfur, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or its hydrolysates, alkyl diaminoethyl glycine chloride solution, triclosan, sodium hypochlorite, Chloramine T, salad powder, iodine compound,
Iodoform, sorbic acid or a salt thereof, salicylic acid, dehydroacetic acid, parahydroxybenzoic acid esters, undecylenic acid, phenol, cresol, p-chlorophenol, p-chloro-m-xylenol, p-chloro-
m-cresol, thymol, phenethyl alcohol, o
Phenylphenol, Irgasan CH3565, halocarban, hexachlorophen, chlorohexidine,
Ethanol, methanol, isopropyl alcohol, benzyl alcohol, ethylene glycol, propylene glycol, phenoxyethanol, chlorobutanol,
Isopropyl methyl phenol, nonionic surfactants (polyoxyethylene lauryl ether, polyoxyethylene nonyl phenyl ether, polyoxyethylene octyl phenyl ether, etc.), amphoteric surfactants, anionic surfactants (sodium lauryl sulfate, potassium lauroyl sarcosine, etc.) ), Cationic surfactants (cetyltrimethylammonium bromide, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride), formaldehyde, hexamine, brilliant green,
Malachite Green, Crystal Violet, Jamal, Photosensitive Element No. 101, Photosensitive Element No. 201, Photosensitive Element 401
, N-long-chain acyl basic amino acid derivatives and their acid addition salts, zinc oxide, hinokitiol, kudin and the like.

(28) Humidifier Glycerin, propylene glycol, 1,3-butylene glycol, hyaluronic acid and its salts, polyethylene glycol, chondroitin sulfate and its salts, water-soluble chitin or chitosan derivatives, pyrrolidone carboxylic acid and its salts, lactic acid Sodium, Mini Sasanishiki extract, etc.

(29) Inhibitors of elastase activity diisopropyl fluorophosphate, plant extracts (Ougon, Hypericum perforatum, Clara, mulberry leaves, calyx, gennoshoko, comfrey, sage, seaweed, bollige, buttonpi) and seaweed extracts.

(30) Hair preparations Selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine, thianthol, castal tincture, ginger tincture, capsicum tincture, quinine hydrochloride, strong aqueous ammonia, potassium bromate, bromate Sodium, thioglycolic acid, etc.

(31) Antiandrogens Estrogen (estrone, estradiol, ethinylestradiol, etc.), isoflavones, oxendrone, etc.

(32) Peripheral vascular blood flow promoter Vitamin E and its derivatives, assembly extract, garlic extract, ginseng extract, aloe extract, gentian extract, touki extract, cepharanthin, carpronium chloride, minoxidil and the like.

(33) Topical stimulants Pepper tincture, vanylamide nonylate, tincture tincture, tincture tincture, peppermint oil, l-menthol, camphor, benzyl nicotinate and the like.

(34) Metabolic activators Photosensitizer No. 301, hinokitiol, pantothenic acid and its derivatives, allantoin, placental extract, biotin, glyceride pentadecanoate, and the like.

(35) Antiseborrheic agents pyridoxine and its derivatives, sulfur, vitamin B6 and the like.

(36) Keratolytic agents Resorcin, salicylic acid, lactic acid and the like.

(37) Oxidizing agent Hydrogen peroxide solution, sodium persulfate, ammonium persulfate, sodium perborate, urea peroxide, sodium percarbonate, sodium tripolyphosphate, sodium bromate, potassium bromate, pyrroline peroxide Sodium citrate, sodium orthophosphate, sodium silicate hydrogen peroxide adduct, sodium sulfate sodium chloride hydrogen peroxide adduct, β-tyrosinase enzyme solution, mushroom extract and the like.

(38) Hair remover Strontium sulfate, sodium sulfide, barium sulfide,
Inorganic reducing agents such as calcium sulfide; thioglycolic acid or salts thereof (calcium thioglycolate, sodium thioglycolate, lithium thioglycolate, magnesium thioglycolate, strontium thioglycolate, etc.).

(39) Hair swelling agent Ethanolamine, urea, guanidine and the like.

(40) Dye agent 5-aminoorthocresol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 1-
Amino-4-methylaminoanthraquinone, 3,3'-
Iminodiphenol, 2,4-diaminophenoxyethanol hydrochloride, 2,4-diaminophenol hydrochloride, toluene-2,5-diamine hydrochloride, nitroparaphenylenediamine hydrochloride, paraphenylenediamine hydrochloride, N-phenylparaphenylenediamine hydrochloride, meta-hydrochloride Phenylenediamine, orthoaminophenol, N-phenylparaphenylenediamine acetate, 1,4-diaminoanthraquinone, 2,6-diaminopyridine, 1,5-dihydroxynaphthalene, toluene-2,5-diamine, toluene
3,4-diamine, nitroparaphenylenediamine, paraaminophenol, paranitroorthophenylenediamine, paraphenylenediamine, paramethylaminophenol, piclamic acid, sodium piclamate,
N, 'N-bis (4-aminophenyl) -2,5-diamino-1, -4-quinonediimine, 5- (2-hydroxyethylamino) -2-methylphenol, N-phenylparaphenylenediamine, metaamino Phenol, metaphenylenediamine, 5-aminoorthocresol sulfate, 2-amino-5-nitrophenol sulfate, orthoaminophenol sulfate, orthochloroparaphenylenediamine sulfate, 4,4'-diaminodiphenylamine sulfate,
2,4-diaminophenol sulfate, toluene-2 sulfate,
5-diamine, nitroparaphenylenediamine sulfate, paraaminophenol sulfate, paranitroorthophenylenediamine sulfate, paranitrometaphenylenediamine sulfate, paraphenylenediamine sulfate, paramethylaminophenol sulfate, metaaminophenol sulfate, metaphenylenediamine sulfate, catechol , Diphenylamine, α
-Naphthol, hydroquinone, pyrogallol, phloroglucin, gallic acid, resorcin, tannic acid, sodium 2-hydroxy-5-nitro-2 ', 4'-diaminoazobenzozen-5'-sulfonate, hematein and the like.

(41) Flavors Natural animal flavors such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, ylang-ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, Guayakwood essential oil, cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, gingergrass essential oil, cedar essential oil, spearmint essential oil, western Mentha essential oil, Great Fengxiang essential oil, Tuberose essential oil, T-shaped essential oil, Orange flower essential oil, Winter green essential oil, Truval sam essential oil, Batturi essential oil, Rose essential oil, Palmarosa essential oil, Cypress essential oil, Hiba essential oil,
Sandalwood essential oil, Petit glen essential oil, Bay essential oil, Vetiva essential oil, Bergamot essential oil, Peruvian balsam essential oil, Boad rose essential oil, Camphor essential oil, Mandarin essential oil, Eucalyptus essential oil, Lime essential oil, Lavender essential oil, Linaloe essential oil, Lemongrass essential oil, Lemon essential oil, Rosemary Vegetable flavors such as essential oils and Japanese peppermint essential oils, and other synthetic flavors.

(42) Dyes / colorants Red cabbage dye, red rice dye, madder dye, anato dye, squid dye, turmeric dye, endu dye, krill dye, persimmon dye, caramel, gold, silver, gardenia dye, corn dye , Onion pigment, tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin, etc.

In addition, humectants, hormones, sequestering agents, pH adjusters, chelating agents, preservatives / anti-binders, antibacterial agents, cooling agents, stabilizers, emulsifiers, animal and vegetable proteins and their decomposed products , Animal and plant polysaccharides and their degradation products,
Vegetable glycoproteins and their degradation products, blood flow enhancers, anti-inflammatory agents / anti-inflammatory agents / anti-allergic agents, cell activators, keratolytic agents,
Includes wound healing agents, hair removers, foam enhancers, thickeners, oral agents, deodorants and deodorants, bitters, seasonings, enzymes, etc. Various effects can be expected.

Further, the dosage form of the elastase activity inhibitor and the cosmetic composition of the present invention is arbitrary, and may be capsule-like, powder-like,
It can be used in the form of granules, solids, liquids, gels, foams, emulsions, creams, ointments, sheets, and other pharmaceuticals, quasi-drugs, and cosmetics for skin and hair.

Specifically, for example, external preparations, basic cosmetics such as lotions, emulsions, creams, ointments, lotions, oils, packs, facial cleansers and skin cleansers, massage agents, cleansing agents, aftershaves Lotions, pre-show lotions, shaving creams, shampoos, rinses, hair treatments, hair styling products, perming agents, hair tonics, hair dyes, hair growth / hair restorations, and other hair / scalp cosmetics, foundations, lipsticks,
Makeup cosmetics such as blusher, eyeshadow, eyeliner, mascara, etc., perfumes, bath preparations, other toothpastes, mouth fresheners / gargles, deodorants / deodorants, sanitary cotton, wet tissues, etc. It can also be used for general foods and drinks.

The method for adding the elastase activity inhibitor and the cosmetic composition of the present invention to the cosmetic composition may be added in advance or during the production, and may be appropriately selected in consideration of workability. good.

[0078]

EXAMPLES The present invention will now be described with reference to production examples, test examples, and prescription examples, but the present invention is not limited to these examples.

(Production Example 1)
100 g of each plant of Shinyi, Touki, Bowie and Forsythia were each extracted with warming in purified water (about 80 ° C.) for about 5 hours, and filtered to obtain an extract (dry solid content: about 0.1 to 2.5% by weight). 1.
Get 0kg.

(Production Example 2)
100 g of each plant of Shiny, Touki, Bowie and Forsythia was immersed in a 50% ethanol solution, extracted at room temperature for 5 days and nights, and then filtered to obtain an extract (dry solid content: about 0.1 to 2.5
% By weight).

(Production Example 3)
100 g of each plant of Shiny, Touki, Bowie and Forsythia are immersed in 70% ethanol solution or 70% 1,3-butylene glycol solution, or 60% propylene glycol solution, or purified water (about 30 ° C) and brought to room temperature. And extracted for 5 days and nights, and then filtered to extract (dry solid content: about 0.1-3.0% by weight)
About 1.0 kg.

(Test 1) Elastase activity inhibitory activity test "Test method and evaluation method" The elastase activity inhibitory activity of the plant extract was determined by measuring the activity of pancreatic elastase and synthetic substrate N-
Evaluation was performed using succinyl-ala-ala-ala-p-nitroanilde. The substrate was adjusted to 0.1 M with dimethyl sulfoxide, and a 1 mM solution diluted 100-fold with a buffer at the time of use was used. The enzyme was adjusted to 0.05 unit / mL with a buffer. 100 μL of substrate was reacted with 50 μL of enzyme and 50 μL of inhibitor at 37 ° C. for 30 minutes, and then 405 nm
The absorbance was measured by using the above formula, and the inhibition rate was calculated by the following equation (1). The buffer used was a 0.2 M Tris-HCl buffer (pH 8.8). The results are shown in FIG. a. Sample The sample for the elastase activity inhibitory activity test was the extract obtained in Production Example 2 of the present invention (adjusted to a solid content of 0.1%).
Was used for the test. As a comparative control, the same concentration of dipotassium glycyrrhizinate was used.

[0083]

## EQU1 ## Inhibition rate (%) = 100− (presence of inhibitor / no inhibitor) × 100

(Test Results) As shown in FIG. 1, the results of the present invention are as follows.
It was confirmed that each plant extract of Forsythia had an effect of significantly suppressing elastase activity.

(Test 2) Safety test (1) Primary skin irritation test Various plant extracts obtained in Production Examples 1 to 3 were prepared with purified water so that the dry solid concentration was about 0.5% by weight. Japanese rabbits with female back shaved (female, 3 per group, weight 2.3kg)
Before and after). The judgment was made 24, 48, and 72 hours after application by the primary irritation scoring method using erythema and edema as indices. The result was negative in all animals without any erythema and edema.

(Test 3) Safety test (2) Acute toxicity test Similarly, powders obtained by concentrating and drying various plant extracts obtained in Production Examples 1 to 3 (dry solid content: about 1.0% by weight) )
Was orally administered to ddy mice (male and female, 5 mice / group, 5 weeks old), which had been fasted for 4 hours before the test, in an amount of 2,000 mg / kg.
The appearance and degree of toxicity symptoms were observed over time. As a result, no abnormality was observed in all mice for 14 days,
In addition, there was no abnormality in the dissection result. Therefore, LD50 is 2,
It was determined to be 000 mg / kg or more.

(Prescription Examples) Production of Elastase Activity Inhibitor and Cosmetic Composition In accordance with the above evaluation results, the following prescription examples are shown. Each prescription example may be one produced by a conventional method in the production of each product. , Only the blending amount is shown. Further, the present invention is not limited to these.

(Formulation Example 1) Emulsion weight% 1. Squalane 5.0 2. Olive oil 5.0 3. Jojoba oil 5.0 4. Cetyl alcohol 1.5 5. Glycerin monostearate 2.0 6. Polyoxyethylene (20) cetyl ether 3.0 7. Poly Oxyethylene (20) soorbitan monooleate 2.0 8.1,3-butylene glycol 1.0 9. Glycerin 2.0 10. A: Koboku hot water extract B: Gomishi hot water extract C: Psycho hot water extract D: Shiny hot water Extract E: Touki hot water extract F: Bowie hot water extract G: Forsythia hot water extract * Any one of A to G 5.0 11. Appropriate perfume and preservative 12. Purified water 100

(Formulation Example 2) Peel-off pack (% by weight) 1. Glycerin 5.0 2. Propylene glycol 4.0 3. Polyvinyl alcohol 15.0 4. Ethanol 8.0 5. Polyoxyethylene glycol 1.0 6. Garnish 50% ethanol extract 3.0 7. Shiny 50 % Ethanol extract 3.0 8. Appropriate amount of fragrance and preservative 9. Residue to purified water 100

(Formulation Example 3) Cold cream weight% 1. Beetle wax 11.0 2. Liquid paraffin 22.0 3. Lanolin 10.0 4. Almond oil 15.0 5. Borax 0.5 6. Psycho 50% ethanol extract 2.0 7. Forsythia 50% Ethanol extract 2.0 8. Appropriate amount of fragrance and preservative 9. Residue to 100 with purified water

(Formulation Example 4) Body soap weight% 1. Potassium laurate 15.0 2. Potassium myristate 5.0 3. Propylene glycol 5.0 4. Kokuboku 60% ethanol extract 0.5 5. Gomishi 60% 1,3-butylene glycol extract Liquid 0.5 6. Shiny 50% ethanol extract 0.5 (Ethanol: 1,3-butylene glycol = 1: 1 extract) 7. Appropriate amount of pH adjuster 8. Appropriate amount of preservative 9. Purified water 100

(Formulation Example 5) Shampoo wt% 1. Triethanolamine lauryl sulfate 5.0 2. Sodium polyoxyethylene lauryl ether sulfate 12.0 3.1,3-butylene glycol 4.0 4. Diethanolamide laurate 2.0 5. Disodium edetate 0.1 6. Bowie hot water extract 1.0 7. Kokuboku hot water extract 1.0 8. Garbage hot water extract 1.0 9. Appropriate amount of fragrance and preservative 10. Residue to 100 purified water

(Formulation Example 6) Rinse weight% 1. Stearyl trimethyl ammonium chloride 2.0 2. Cetostearyl alcohol 2.0 3. Polyoxyethylene lanolin ether 3.0 4. Propylene glycol 5.0 5. Bowie 30% ethanol extract 1.0 6. Touki heat Water extract 1.0 7. Appropriate amount of pH adjuster 8. Appropriate amount of preservative 9. Residual to make purified water 100

(Formulation Example 7) Hair liquid% by weight 1. Ethanol 29.0 2. Polyoxypropylene butyl ether phosphoric acid 10.0 3. Polyoxypropylene monobutyl ether 5.0 4. Triethanolamine 1.0 5. Garnish hot water extract 1.0 6. Shinyi Hot water extract 1.0 7.Appropriate preservative 8.Purified water 100 residue

(Formulation Example 8) Hair tonic weight% 1. Ethanol 40.0 2. Ethyl oleate 1.0 3. Polyoxyethylene (40) hydrogenated castor oil 2.0 4. Shiny 30% ethanol extract 1.0 5. Dish 30% ethanol extraction Liquid 1.0 6. Bowie 30% ethanol extract 1.0 7. Appropriate amount of fragrance and preservative

(Formulation Example 9) Granule bath agent weight% 1. Sodium bicarbonate 58.0 2. Anhydrous sodium sulfate 30.0 3. Borax 3.0 4. Dust hot water extract 5.0 5. Touki hot water extract 2.0 6. Psycho heat Water extract 2.0

(Formulation Example 10) Coating protective agent Gauze or liniment cloth was used to add hot water extract of Shinoki 30
Impregnated with a prescription solution containing an appropriate amount of ethanol extract, antibiotics, anti-inflammatory, etc., and attach it to the wound. Alternatively, the hot water extract of Touki and the 30% ethanol extract of Shiny may be directly sprayed locally and covered with gauze or the like.

(Test 4) Use Effect Test The effect when the cosmetic composition of the present invention was actually used was examined. Use test is 25 healthy adult women
A panel of 30 50-year-olds each day, morning and night
The washing was performed by applying an appropriate amount of the emulsion of Formulation Example 1 to the face over three months after washing the face. As a control, a preparation prepared by removing a plant extract of the present invention from the emulsion in the same manner was used. The evaluation was performed according to the following criteria. The results are as shown in Table 1 and the numerical values in the table represent the number of persons.
No one complained of skin abnormalities during the use period.

"Improvement effect of wrinkles and fine wrinkles" Effective: Wrinkles and fine wrinkles became less noticeable. Slightly effective: wrinkles and small wrinkles are slightly less noticeable. Ineffective: No change from before use.

"Effect of improving skin gloss and firmness" Effective: Skin gloss and firmness increased. Slightly effective: Gloss and firmness of skin increased slightly. Ineffective: No change from before use.

[0101]

[Table 1]

(Test results) As shown in Table 1, the results of the present invention are as follows.
It was confirmed that the latex containing the plant extracts of Forsythia significantly gave gloss and firmness to the skin and also reduced wrinkles and fine wrinkles.

[0103]

EFFECT OF THE INVENTION The elastase activity inhibitor or the cosmetic composition of the present invention containing one or more plant extracts selected from Kokuboku, Domishi, Psycho, Shini, Touki, Bowie and Forsythia has an elastase activity inhibitory effect. It is confirmed that the skin has luster and shine on the skin, can prevent aging, and can also reduce wrinkles and wrinkles and maintain a youthful skin condition. In addition, the present invention
It can also be used for general foods and drinks.

[Brief description of the drawings]

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1.
It is a figure which shows the elastase activity inhibition rate of each plant extract of touki, bowie, and forsythia.

Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat II (reference) A61K 7/50 A61K 7/50 A61P 17/00 31/00 617 43 / 00643D A61K 35/78 35/78 C N W F-term (reference) 4C083 AA082 AA111 AA112 AA122 AB152 AB312 AB352 AC022 AC072 AC102 AC122 AC182 AC242 AC422 AC432 AC442 AC532 AC542 AC642 AC692 AC782 AC902 AD042 AD512 BB51 CC01 CC02 CC05 CC07 CC23 CC25 CC32 CC37 CC38 CC39 DD12 DD12 DD16 DD12 DD16 DD12 AB64 AB65 AC03 AC04 AC05 AC06 AC11 AC13 BA08 BA09 BA10 MA07 MA63 NA14 ZA89 ZC20

Claims (2)

[Claims] (1) An elastase activity inhibitor comprising one or more plant extracts selected from Kokuboku, Domishi, Psycho, Shinyi, Touki, Bowie and Forsythia. 2. A cosmetic composition comprising the elastase activity inhibitor according to claim 1.