CN115067880A - Fluorescent signal detection system and detection method - Google Patents
Fluorescent signal detection system and detection method Download PDFInfo
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- CN115067880A CN115067880A CN202210653274.5A CN202210653274A CN115067880A CN 115067880 A CN115067880 A CN 115067880A CN 202210653274 A CN202210653274 A CN 202210653274A CN 115067880 A CN115067880 A CN 115067880A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
Abstract
The invention discloses a fluorescence signal detection system and a detection method. The fluorescence excitation light source is used for generating excitation light required by excitation of a fluorescent material; the probe is used for receiving and emitting the exciting light and acquiring a fluorescent signal generated by exciting the fluorescent material; the detection unit is used for collecting the fluorescent signal acquired by the probe and converting the fluorescent signal into fluorescent information which can be displayed; the display unit is in communication connection with the detection unit and displays the fluorescence information. The fluorescence signal detection system of the invention can realize the detection of the focus part of the organism of a freely moving organism (living body, non-anesthesia) in a non-destructive way.
Description
Technical Field
The present invention relates to the field of fluorescence signal imaging technology, and more particularly, to a fluorescence signal detection system and method.
Background
As a new living body imaging means, the near infrared (NIR, 650-1700nm) fluorescence imaging technology not only has the advantages of rapid imaging, multi-channel signal acquisition, high sensitivity, no radiation and other optical imaging, but also overcomes the limitations of limited penetration depth and biological autofluorescence interference and the like of the traditional fluorescence imaging, and can provide structural and functional information with high in-vivo space-time resolution. Taking indocyanine green (ICG) as an example, the near-infrared fluorescence imaging technology has been widely applied in the fields of lymph node biopsy, surgical navigation, and the like. Further, with the rapid development of molecular imaging technology and nano-drug research, various composite near-infrared fluorescent probes also play an important role in accurate diagnosis and treatment in molecular detection and pathological typing of clinical samples. In addition, in many application scenes, indexes such as spectral information, fluorescence signal intensity and the like can also provide abundant and intuitive diagnosis bases for clinic.
Various fluorescence imaging devices developed at present are large in size, complex to operate and poor in portability, so that the imaging angle and method are limited, and higher requirements are provided for the matching degree of patients; in addition, the compound fluorescence image information still needs to be processed and analyzed in the later period, and the popularization of the fluorescence imaging technology in clinical application is limited.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.
Disclosure of Invention
The invention aims to provide a fluorescence signal detection system and a detection method, wherein the detection system can realize the detection of the focus part of a freely moving organism (living body and non-anesthesia) in a non-destructive mode.
In order to achieve the above object, an embodiment of the present invention provides a fluorescence signal detection system, which includes a fluorescence excitation light source, a probe, a detection unit, and a display unit.
The fluorescence excitation light source is used for generating excitation light required by excitation of a fluorescent material;
the probe is used for receiving and emitting the exciting light and acquiring a fluorescent signal generated by exciting the fluorescent material;
the detection unit is used for collecting the fluorescent signal acquired by the probe and converting the fluorescent signal into fluorescent information which can be displayed;
the display unit is in communication connection with the detection unit and displays the fluorescence information.
In one or more embodiments of the invention, the probe has an absorbent structure that is securable to a skin surface.
In one or more embodiments of the invention, the suction structure of the probe comprises a suction cup, a patch, or a clip.
In one or more embodiments of the invention, an optical lens group capable of homogenizing excitation light is arranged in the probe.
In one or more embodiments of the present invention, the system further includes a flexible bidirectional optical transmission module disposed between the probe, the fluorescence excitation light source, and the detection unit.
In one or more embodiments of the present invention, the bidirectional optical transmission module includes a flexible optical transmission body, and the optical transmission body is connected between the fluorescence excitation light source and the probe and between the probe and the detection unit, so as to transmit the excitation light to the probe and transmit the fluorescence signal acquired by the probe to the detection unit.
In one or more embodiments of the present invention, the system further includes a filter module positioned in front of the detection unit, and the filter module is configured to filter and remove the stray light signals mixed with the fluorescence signals acquired by the probe, so that the detection unit can acquire fluorescence signals with high signal-to-noise ratio.
In one or more embodiments of the present invention, the filter module includes a plurality of dielectric films or absorption films for filtering or absorbing the parasitic light signal, and the filter module can be used for passing through a single-wavelength or multi-wavelength fluorescent signal.
In one or more embodiments of the present invention, the system further includes a control unit, connected to the fluorescence excitation light source and the detection unit, for controlling a start-stop time, a pulse frequency and an optical power of the excitation light emitted from the fluorescence excitation light source, and controlling the detection unit to acquire the fluorescence signal at a certain time frequency.
In one or more embodiments of the present invention, the detection unit further includes an input/output module, and the input/output module is connected to the control unit and the display unit, and is configured to receive a control command sent by the control unit and transmit identifiable fluorescent information to the display unit.
In one or more embodiments of the present invention, the system further includes a trigger switch, where the trigger switch is connected to the control unit and is used to control the on/off of the signal of the control unit, so that the control unit can control the fluorescence excitation light source to emit excitation light, and the trigger switch includes a pedal switch, a push-button switch, and a trigger switch.
In one or more embodiments of the present invention, the fluorescence signal response band of the detection unit is 900-1700nm, and the detection unit can convert the fluorescence signal of the band into fluorescence information recognizable by the display unit, where the fluorescence information includes fluorescence image information, fluorescence intensity information, and fluorescence spectrum information.
In one or more embodiments of the present invention, the fluorescence excitation light source includes a laser, a solid-state light source, and an LED light emitting device, a coverage wavelength band of excitation light emitted by the fluorescence excitation light source is 350-1700nm, and the excitation light is pulse excitation light with a tunable wavelength.
The invention also provides a fluorescence signal detection method, which comprises the following steps:
applying a fluorescent material to the inside of the living body to mark a lesion site;
guiding exciting light to a focus part of an organism and exciting the fluorescent material;
collecting fluorescence signals generated by exciting the fluorescent materials, and converting the fluorescence signals into displayable fluorescence information.
In one or more embodiments of the present invention, the wavelength band of the excitation light is in the range of 500nm to 1550 nm.
In one or more embodiments of the invention, the method further comprises filtering the excitation light signal mixed in the fluorescence signal.
In one or more embodiments of the invention, the fluorescent signal is collected at a temporal frequency.
Compared with the prior art, the fluorescence signal detection system provided by the embodiment of the invention can realize nondestructive and in-situ lesion detection on organisms under the condition that the normal physiological state of the organisms is not influenced.
According to the fluorescence signal detection system provided by the embodiment of the invention, the probe is provided with the adsorption structure, so that the probe can be fixedly attached and contacted with a detected organism, and even under the condition of organism movement, the excitation and acquisition of a fluorescence signal can be more stable, and the focus detection accuracy is more facilitated.
The fluorescence signal detection system provided by the embodiment of the invention is provided with the flexible optical transmission body, has the characteristics of light weight and flexibility, can move along with the movement of the detected organism, and can realize the detection of the focus part of the organism of the freely moving organism (living body and non-anesthesia) in a non-destructive manner by matching with the probe with the adsorption structure.
The fluorescence signal detection system provided by the embodiment of the invention has the advantages of high penetration depth and high signal-to-noise ratio of 900-1700nm band fluorescence, and the acquired fluorescence information comprises imaging and spectrum, so that the focus information is more comprehensive.
The fluorescence signal detection system of the embodiment of the invention can realize the nondestructive focus detection of the organism, and the normal physiological activity of the organism does not influence the stability of the fluorescence signal in the detection process, so that the system has better and wider application prospect in the fields of clinical focus detection of the organism and the like.
Drawings
FIG. 1 is a schematic diagram of a fluorescent signal detection system according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a fluorescence signal detection method according to an embodiment of the present invention.
Detailed Description
The following detailed description of the present invention is provided in conjunction with the accompanying drawings, but it should be understood that the scope of the present invention is not limited to the specific embodiments.
Throughout the specification and claims, unless explicitly stated otherwise, the word "comprise", or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated element or component but not the exclusion of any other element or component.
As shown in fig. 1, an embodiment of the invention provides a fluorescence signal detection system, which includes a fluorescence excitation light source 10, a probe 20, a detection unit 30, a filter module 40, a light transmission structure 50, a control unit 60, and a display unit 70.
The fluorescence excitation light source 10 is used to generate excitation light required for exciting the fluorescent material, and the excitation light is pulse excitation light with adjustable wavelength. The excitation light wave band covers 350-1700nm, preferably 500-1550 nm. The fluorescence excitation light source 10 may be a laser, a solid state light source, and a narrow band LED light emitting device, such as 808 laser.
The probe 20 is connected to the fluorescence excitation light source 10 through the light transmission structure 50, and is used for transmitting the excitation light to the living body and acquiring a fluorescence signal in the living body excited by the excitation light. The probe 20 may have a detection housing with a light transmission port formed therein for passing the excitation light. An optical lens group for increasing the transmission of 400-1550 nm waveband can be arranged in the detection shell, and the optical lens group can uniformly and efficiently pass excitation light of 500-1550nm waveband through the light transmission port and irradiate the surface of an organism. The light transmission port of the detection shell is provided with an adsorption structure which can be fixedly attached and contacted with an organism, and the adsorption structure can be attached to the skin surface of the detected part of the organism in a harmless sucking disc or adhesion mode of the organism.
In one embodiment, the suction structure comprises a suction cup, a patch, or a clip. When the probe 20 is used, it is fixedly attached to the surface of the living body by the attaching structure. The biological surface is the surface of the skin of the living body at the nearest part of the lesion site of the living body marked by a fluorescent probe (fluorescent material). The probe 20 transmits excitation light to the biological lesion site marked by the fluorescent probe, and acquires a fluorescent signal of the biological lesion site marked by the fluorescent probe. Wherein, the focus site of the organism marked by the fluorescent probe is the site needing to be detected in the organism, such as organs like kidney, liver, mammary gland, carotid artery, etc., and the probe emitting the fluorescence of 900-1700nm wave band is specifically combined at the site by means of intravenous injection, in-situ injection, etc.
The detection unit 30 is used to collect the fluorescence signals acquired by the probe 20 and convert the fluorescence signals into fluorescence information that can be displayed. The fluorescence signal response band of the detection unit 30 is 900-1700nm, and the detection unit 30 can convert the fluorescence signal of the band into fluorescence information that can be recognized by the display unit 70, where the fluorescence information includes fluorescence image information, fluorescence intensity information, and fluorescence spectrum information. In a specific embodiment, the detecting unit 30 can be any device capable of converting the fluorescence signal with the wavelength band of 900-.
The detecting unit 30 further comprises an input/output module, which is connected to the control unit 60 and the display unit 70, and is used for receiving a control command sent by the control unit 60 and transmitting fluorescence information, such as fluorescence intensity image and fluorescence spectrum image information, which can be recognized by the display unit 70 to the display unit 70.
The optical filter module 40 is disposed in front of the detection unit 30, and the optical filter module 40 is used for filtering and removing the stray light signal mixed with the fluorescence signal acquired by the probe 30, so that the detection unit 30 can acquire the fluorescence signal with a high signal-to-noise ratio. The filter module 40 can be switched manually or electrically, and typically comprises a 900nm long-pass filter, a 1000nm long-pass filter, a 1300nm long-pass filter, etc. with a diameter of 10-55 mm. The optical filter module 40 includes a plurality of dielectric films or absorption films for filtering or absorbing the stray light signals, and can filter the excitation light obtained by the probe 20 and efficiently transmit the fluorescence signals. The filter module 40 can be used for the fluorescent signals of a single wave band or a plurality of wave bands to pass through.
The optical transmission structure 50 includes a flexible optical transmission body 51, the optical transmission body 51 is connected between the fluorescence excitation light source 10 and the probe 20, and between the probe 20 and the optical filter module 40 and the detection unit 30, so as to transmit the excitation light to the probe 20, and transmit the fluorescence signal obtained by the probe 20 to the optical filter module 40 and the detection unit 30. The two-way transmission of the excitation light and the fluorescence signal can be carried out in the same channel or in different directions along the side axis of the two channels. The flexible optical transmission body 51 is mainly characterized by light weight, flexibility, and capability of moving along with the movement of the detected organism, and can be matched with the probe 20 with an adsorption structure to realize the detection of the focus part of the organism of the freely moving organism (living body, non-anesthesia) in a non-destructive manner. The flexible optical transmission body 51 may be, for example, a quartz optical fiber bundle, and may have a single core diameter or a multi-core diameter.
The control unit 60 is connected to the fluorescence excitation light source 10 and the detection unit 30, and is configured to control the start-stop time, the pulse frequency, and the optical power of the excitation light emitted from the fluorescence excitation light source 10, and control the detection unit 30 to collect fluorescence signals at a certain time frequency. The control unit 60 is, for example, a multifunctional I/O device such as an acquisition card, and implements computer language control by software programming such as labview, and a plurality of electrical signal transmitting and receiving interfaces transmit and receive TTL signals and clock the transmit and receive TTL signals in sequence.
The control unit 60 is further connected with a trigger switch, the trigger switch is used for controlling the control unit 60 to send a trigger signal for generating exciting light to the fluorescence excitation light source 10, so that the control unit 60 can be controlled (manually controlled in the detection process) to control the fluorescence excitation light source 10 to emit exciting light, and the trigger switch can include a pedal switch, a key switch and a trigger switch.
The display unit 70 is communicatively connected to the detection unit 30, and is used for receiving and displaying the fluorescence information converted by the detection unit 30. The display unit 70 may also process the fluorescence information to facilitate medical diagnosis. The display unit 70 is, for example, a computer host or a computer display unit.
The invention also provides a fluorescence signal detection method, which comprises the following steps: applying a fluorescent material to the inside of the living body to mark a lesion site; guiding the exciting light with the covering wave band of 500nm-1550nm to the focus part of the organism and exciting the fluorescent material; collecting fluorescent signals generated by exciting the fluorescent material at a certain time frequency, filtering the exciting light signals mixed in the fluorescent signals, and converting the fluorescent signals into displayable fluorescent information.
Referring to FIG. 2, in the following embodiment, the fluorescence signal detection method of the present invention is explained in conjunction with FIG. 2.
The probe 20 is first fixed to the skin surface of the living body at the closest point to the site to be probed. Due to the presence of the adsorption structure of the probe 20, the adsorption process can ensure that the normal physiological characteristics of the organism are not affected.
The fluorescent probe a (such as ICG, Ag) with the wave band of 900-1700nm is injected by vein injection or in situ injection 2 S, etc.) is delivered into the organism, and the fluorescent probe a enables the focus g to be detected of the organism to be imaged.
The control program b (the control program b may be set manually so that the control unit 60 controls the corresponding components to cooperate with each other according to the control program b) enables the control unit 60 to send the TTL signal c to the fluorescence excitation light source 10 and send the TTL synchronous control signal d to the detection unit 30, so that the fluorescence excitation light source 10 and the detection unit 30 operate according to the working procedures designed by the control program b, and the specific working procedures are as follows:
the fluorescence excitation light source 10 generates excitation light h, the excitation light h is transmitted by optical fiber coupling and sequentially passes through the flexible light transmission body 51 and the probe 20, and the probe 20 uniformly irradiates a focus part g to be measured of an organism;
the focus part g to be detected of the organism is enriched with the 900-1700nm waveband fluorescent probe a, so the focus part g to be detected of the organism can generate a fluorescent signal e;
the fluorescence signal e is transmitted through the probe 20, the flexible optical transmission body 51 and the optical filter module 40 in sequence, and enters the detection unit 30(900-1700nm fluorescence signal detector) with high signal-to-noise ratio;
the detection unit 30(900-1700nm fluorescence signal detector) acquires the fluorescence signal e at a certain time interval (for example, 30s) under the instruction of the control unit 60, and converts the fluorescence signal e into fluorescence information f (such as fluorescence intensity image and fluorescence spectrum image) recognizable by the display unit 70;
the display unit 70 processes the acquired fluorescence information f with time interval and presents the processed fluorescence information f to a tester (doctor), and the tester (doctor) judges the focus condition of the to-be-detected part of the organism according to the difference of the fluorescence information f with the time interval.
Compared with the prior art, the fluorescence signal detection system provided by the embodiment of the invention can realize nondestructive and in-situ lesion detection on organisms under the condition that the normal physiological state of the organisms is not influenced.
According to the fluorescence signal detection system provided by the embodiment of the invention, the probe is provided with the adsorption structure, so that the probe can be fixedly attached and contacted with a detected organism, and even under the condition of organism movement, the excitation and acquisition of a fluorescence signal can be more stable, and the focus detection accuracy is more facilitated.
The fluorescence signal detection system provided by the embodiment of the invention is provided with the flexible optical transmission body, has the characteristics of light weight and flexibility, can move along with the movement of the detected organism, and can realize the detection of the focus part of the organism of the freely moving organism (living body and non-anesthesia) in a non-destructive manner by matching with the probe with the adsorption structure.
The fluorescence signal detection system provided by the embodiment of the invention has the advantages of high penetration depth and high signal-to-noise ratio of 900-1700nm band fluorescence, and the acquired fluorescence information comprises imaging and spectrum, so that the focus information is more comprehensive.
The fluorescence signal detection system of the embodiment of the invention can realize the nondestructive focus detection of the organism, and the normal physiological activity of the organism does not influence the stability of the fluorescence signal in the detection process, so that the system has better and wider application prospect in the fields of clinical focus detection of the organism and the like.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (10)
1. A fluorescence signal detection system, comprising:
the fluorescence excitation light source is used for generating excitation light required by the excitation of the fluorescent material;
the probe is used for receiving and emitting the exciting light and acquiring a fluorescent signal generated by exciting the fluorescent material;
the detection unit is used for acquiring the fluorescent signal acquired by the probe and converting the fluorescent signal into fluorescent information which can be displayed;
and the display unit is in communication connection with the detection unit and displays the fluorescence information.
2. The fluorescence signal detection system of claim 1, wherein said probe has an absorbent structure that is securable to a skin surface.
3. The fluorescence signal detection system of claim 2, wherein said attachment structure comprises a suction cup, a patch, or a clip.
4. The fluorescence signal detection system of claim 1, wherein said probe has an optical lens assembly disposed therein for homogenizing the excitation light.
5. The fluorescence signal detection system of claim 1, further comprising a flexible bi-directional light transmission module disposed between said probe, said fluorescence excitation light source, and said detection unit.
6. The fluorescence signal detection system of claim 1, further comprising a filter module disposed between the probe and the detection unit.
7. A method of detecting a fluorescent signal, comprising:
applying a fluorescent material to the inside of the living body to mark a lesion site;
guiding exciting light to a focus part of an organism and exciting the fluorescent material;
collecting fluorescence signals generated by exciting the fluorescent materials, and converting the fluorescence signals into displayable fluorescence information.
8. The method for detecting a fluorescent signal according to claim 7, wherein the wavelength band of the excitation light is in the range of 500nm to 1550 nm.
9. The fluorescence signal detection method according to claim 7, further comprising filtering the excitation light signal mixed in the fluorescence signal.
10. The method for detecting a fluorescent signal according to claim 7, wherein the fluorescent signal is collected at a certain temporal frequency.
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| CN202210653274.5A CN115067880A (en) | 2022-06-09 | 2022-06-09 | Fluorescent signal detection system and detection method |
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| CN202210653274.5A CN115067880A (en) | 2022-06-09 | 2022-06-09 | Fluorescent signal detection system and detection method |
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