CN110916728A - Puncture biopsy method and device based on optical fiber transmission type fluorescence life guidance - Google Patents
Puncture biopsy method and device based on optical fiber transmission type fluorescence life guidance Download PDFInfo
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- A61B10/02—Instruments for taking cell samples or for biopsy
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- A61B17/34—Trocars; Puncturing needles
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- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
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- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
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Abstract
The invention discloses a puncture biopsy method and a device based on optical fiber transmission type fluorescence life guidance, comprising (a) an optical fiber transmission type fluorescence life detection device irradiates pulse laser on a living tissue to be detected after the pulse laser is transmitted by an excitation optical fiber; (b) a fluorescence signal excited from the living tissue to be detected is received by a collecting optical fiber of the detection device and enters a fluorescence detection module to obtain fluorescence life information and fluorescence spectrum information; (c) identifying whether the tissue is pathological or not; (d) moving the detection device to detect the position, repeating the steps a to c, and detecting the position of the lesion tissue; (e) and performing puncture biopsy, selecting a proper puncture point, adjusting the position, the angle and the direction of the biopsy device, triggering the biopsy device to puncture, and taking out the pathological tissue. The invention carries out high-sensitivity detection on the weak autofluorescence spectrum and the fluorescence life of the living tissue, can distinguish the pathological change tissue and the normal tissue from the microscopic level of cell pathophysiology and the like, and then triggers the puncture biopsy to obtain the pathological change tissue.
Description
Technical Field
The invention belongs to the technical field of biomedicine, and relates to a biopsy device based on tissue chemical component detection of a living body.
Background
With the development of science and technology and the increasing demand of people on quality of life, the demand of people on medical technology is increasing day by day. The conventional needle biopsy device is basically implemented based on personal experience of a doctor, and certain missed detection may be caused due to small focus or insufficient experience of the doctor, which may result in failed needle biopsy and even affect the treatment of a patient. Therefore, the pathological tissue and the normal tissue can be distinguished through real-time imaging, the pathological tissue is accurately positioned, and then the puncture biopsy is triggered to obtain the pathological tissue, so that the method has important significance for diagnosis and treatment of patients.
The following are commonly used in the current image-guided biopsy device clinically: (1) the puncture path navigation equipment under the indication of visible light or infrared light is mainly used for path planning and indication, and an optical image cannot distinguish a pathological tissue from a normal tissue from microscopic levels of cell pathophysiology and the like; (2) the puncture device under the ultrasonic guidance can perform real-time imaging, but the image resolution of ultrasonic imaging is not high, and the accurate positioning of the puncture device can be influenced by the sound shadow of the needle point; (3) the puncture under the CT guide is provided with puncture equipment, and although the puncture under the CT guide can accurately display the anatomical structure of tissues, the puncture under the CT guide cannot display the pathophysiological change of pathological change tissues, cannot acquire images in real time and also has ionizing radiation; (4) magnetic resonance guided puncture is also increasingly applied to clinic, but magnetic resonance can carry out functional imaging, but the real-time imaging cannot be carried out, the imaging time is long, all instruments need to be compatible with magnetic resonance, and therefore the application scene is greatly influenced, and the cost is high.
Multispectral spectroscopic detection and analysis has become a fundamental measurement tool in fundamental research due to its very high sensitivity, molecular specificity and non-contact measurement properties. The spectrum detection and analysis are widely applied to the related industrial fields of component detection of various pollutants, food component detection, industrial raw material component detection, petroleum crude oil component detection and the like. In the medical field, the fluorescence spectrum technology and the fluorescence microscopic imaging technology provide a brand new direction for real-time imaging and detection of tumors and cancers, and are expected to be developed into medical technologies suitable for clinical diagnosis. It follows that spectroscopic detection techniques are a very practical and promising means of optical detection. Generally, fluorescence measurement or imaging techniques mostly measure fluorescence intensity, which is easily affected by many factors such as excitation light intensity, sample quenching, and fluorescent dye distribution and concentration, and thus it is difficult to perform quantitative measurement. In contrast, fluorescence lifetime measurement is not affected by factors such as excitation light intensity, fluorophore concentration, photobleaching, and the like, is not limited by other factors that limit intensity measurement, and is only closely related to the microenvironment in which the fluorophore is located. Since the fluorescence lifetime and the fluorescence spectrum are sensitive to the microenvironment in which the fluorescent molecule is located and the type of fluorophore molecule, respectively. If the two can be combined, complementary functional information can be provided for biomedical detection and analysis.
Most of fluorescent detection instruments in the market at present adopt cameras to detect fluorescent information with different wavelengths, and have the problems of low sensitivity, portability and the like. Particularly, when the autofluorescence lifetime of a substance to be detected is measured, the fluorescence signal emitted by each pulse is very weak, and the fluorescence lifetime is also very short, so that a spectrum detection instrument based on a camera for measuring fluorescence information cannot capture the weak and short fluorescence information at all. If used for needle biopsy, it must be considered that the instrument is portable and that the detection process is rapid and simple.
Disclosure of Invention
In order to solve the technical problems, the invention provides a needle biopsy method and a device based on optical fiber transmission type fluorescence lifetime guidance, which can detect the weak autofluorescence spectrum and fluorescence lifetime of living tissues with high sensitivity, can distinguish diseased tissues and normal tissues from the microscopic level of cell pathophysiology and the like, and then trigger the needle biopsy to obtain the diseased tissues.
The above purpose is realized by the following technical scheme:
the biopsy aspiration method based on the optical fiber transmission type fluorescence life guidance is characterized in that:
(a) the optical fiber transmission type fluorescence life detection device transmits pulse laser through an excitation optical fiber and irradiates the living tissue to be detected;
(b) the fluorescence signal excited from the living tissue to be detected is received by the collecting optical fiber of the optical fiber transmission type fluorescence lifetime detection device and enters the fluorescence detection module to obtain fluorescence lifetime information and fluorescence spectrum information of the living tissue to be detected;
(c) identifying whether the living tissue to be detected is a pathological tissue or not according to the detected fluorescence lifetime information and fluorescence spectrum information;
(d) moving the optical fiber transmission type fluorescence life detection device to detect the position, repeating the steps a to c, and detecting the position of the pathological change tissue;
(e) and performing puncture biopsy according to the detected position of the pathological tissue, selecting a proper puncture point, adjusting the position, the angle and the direction of the biopsy device, triggering the biopsy device to puncture, and taking out the pathological tissue.
Preferably, when the optical fiber transmission type fluorescence lifetime detecting device detects whether the tissue to be detected is a lesion tissue, the steps are as follows:
(a) transmitting and irradiating pulse laser on the living body tissue to be detected through an excitation optical fiber, wherein one end of the excitation optical fiber receives the pulse laser coupled by a pulse laser light source, and the other end of the excitation optical fiber guides the pulse laser out and irradiates the living body tissue to be detected;
(b) transmitting fluorescence excited from living body tissue to be measured by collecting optical fiber, dividing into N paths by using light splitting system, filtering each divided path of fluorescence by band pass filters with different central wavelengths, wherein the central wavelength corresponding to each band pass filter is lambdanBand pass width of δ λnWherein N is 1,2, …, N;
(c) measuring the fluorescence light intensity information which changes along with time under the corresponding wavelength and is filtered by each band-pass filter by using N light intensity detectors respectively, and obtaining the fluorescence service life under the corresponding wavelength and a relative fluorescence intensity spectrum between different wavelengths after data analysis;
(d) and identifying whether the living tissue to be detected is the pathological change tissue or not by the fluorescence lifetime of the living tissue to be detected under different wavelengths and the relative fluorescence intensity spectrum information among different wavelengths.
Preferably, in identifying whether the living tissue to be tested is a diseased tissue: and identifying whether the living tissue to be detected is a pathological change tissue or not through the fluorescence life of the living tissue to be detected under different wavelengths.
Preferably, the excitation and collection fibers are replaced by fiber bundles: one optical fiber in the optical fiber bundle is used as an excitation optical fiber to transmit pulse laser, and the rest M optical fibers in the optical fiber bundle are used as collecting optical fibers to collect fluorescence excited from the substance to be detected; and the M optical fibers are transmitted and then combined at the tail ends, and the collected fluorescence is combined and then transmitted to the light splitting system.
Preferably, the excitation fiber, collection fiber and splitting system are replaced by a fiber bundle: one optical fiber in the optical fiber bundle is used as an excitation optical fiber to transmit pulse laser, the rest N optical fibers in the optical fiber bundle are used as collecting optical fibers to collect fluorescence excited from a substance to be detected, the N optical fibers are separated after transmission to obtain N paths of collected fluorescence, and then the N paths of collected fluorescence are filtered by N band-pass filters with different central wavelengths.
Preferably, the needle biopsy method adjusts the type, length, diameter, operation mode and material of the biopsy needle of the needle biopsy device according to the classification of tissues, biopsy sites, the size of a sample obtained from a material and the biopsy sampling mode during the needle biopsy, and provides a longitudinally extending fluid channel connection by means of a joint at the operation end of the handle.
Puncture biopsy device based on guide of optic fibre transmission type fluorescence life-span, its characterized in that: the device comprises an optical fiber transmission type fluorescence life detection device and a biopsy device, wherein the optical fiber transmission type fluorescence life detection device comprises a pulse laser, an excitation optical fiber, a collection optical fiber and a fluorescence detection module; the biopsy device includes a handle and a biopsy needle comprised of a core and a coaxial catheter.
Preferably, the fluorescence detection module of the optical fiber transmission type fluorescence lifetime detection device comprises a light splitting system, N band-pass filters, N light intensity detectors, a signal collector and a computer; the fluorescence signal entering the fluorescence detection module is divided into N paths by a light splitting system, N band-pass filters respectively filter N paths of fluorescence, N light intensity detectors respectively detect fluorescence light intensity information which is filtered by each band-pass filter and changes along with time under corresponding central wavelength, the signal collector converts the fluorescence light intensity information detected by each light intensity detector and transmits the converted fluorescence light intensity information to the computer, and the computer analyzes the converted fluorescence light intensity information to obtain the fluorescence life and relative fluorescence intensity spectrum between different wavelengths.
Preferably, the excitation and collection fibers are replaced by fiber bundles: one optical fiber in the optical fiber bundle is used as an excitation optical fiber to transmit pulse laser, and the rest M optical fibers in the optical fiber bundle are used as collection optical fibers to collect fluorescence excited from a substance to be detected; the M optical fibers are transmitted and then merged at the tail end, and the collected fluorescence is merged and then transmitted to the fluorescence detection module.
Preferably, the excitation fiber, collection fiber and splitting system are replaced by a fiber bundle: one optical fiber in the optical fiber bundle is used as an excitation optical fiber to transmit pulse laser, the rest N optical fibers in the optical fiber bundle are used as collecting optical fibers to collect fluorescence excited from a substance to be detected, the N optical fibers are separated after transmission to obtain N paths of collected fluorescence, and then the N paths of collected fluorescence are filtered by N band-pass filters with different central wavelengths.
The invention has the following innovation points:
1. the service life of fluorescence under different wavelengths is detected by using a plurality of light intensity detectors, and the detection speed and the accuracy of the system are obviously improved based on the detection of histochemical components and pathological characteristics of the living body;
2. the invention simultaneously detects the fluorescence life of the substance to be detected under different wavelengths and the relative fluorescence intensity spectrum information among different wavelengths, has large information amount and can obviously improve the accuracy rate;
3. the optical fiber and the optical fiber bundle are utilized to guide the pulse laser and transmit the excited fluorescence, so that the flexibility of the detection system is improved, and the sensor can be conveniently operated in a handheld manner in different application scenes;
4. the invention utilizes the autofluorescence of the tissue to be detected to analyze the chemical components and the pathophysiological characteristics of the tissue, and a fluorescent marker is not needed in the detection process, so the detection process is very convenient.
Drawings
FIG. 1 is a schematic diagram of a fiber-optic transmission-based fluorescence lifetime-guided needle biopsy method of the present invention;
FIG. 2 is a schematic diagram of a beam splitting system of the present invention with a collimating lens, a beam splitter and a mirror;
FIG. 3 is a schematic diagram of a spectroscopy system of the present invention utilizing a collimating lens and a grating;
FIG. 4 is a schematic diagram of an embodiment of a needle biopsy method based on fiber-optic transmission-type fluorescence lifetime guidance;
wherein: 1-biopsy needle, 2-optical fiber, 3-excitation optical fiber, 4-collection optical fiber, 5-handheld handle, 6-manual trigger biopsy button, 7-pulse laser light source, 8-light splitting system, 9-computer and image display module, 10-collimating lens, 11-first spectroscope, 12-second spectroscope, 13-third spectroscope, 14-reflector, 15-grating, 16-tissue, 17-fluid channel joint, 18-needle core, 19-coaxial catheter, 20-sample groove and 21-fluid channel.
Detailed Description
The present invention will be described in further detail with reference to the following drawings and examples, it being understood that the specific embodiments described herein are merely illustrative of the invention and are not to be construed as limiting the invention. In addition, the technical features of the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
Example 1
When biopsy is performed during operation, the present example uses four photomultiplier tubes as light intensity detectors to detect fluorescence signals of multiple wavelengths such as 400nm, 450nm, 530nm, and 580nm, respectively, which are excited from the living tissue to be measured, and identifies the components of the substance to be measured according to the measured fluorescence lifetime and the relative fluorescence intensity spectrum information between different wavelengths. In the present embodiment, the needle biopsy method based on fiber-optic transmission fluorescence lifetime guidance is implemented as the device shown in fig. 1, and the identification of the property of the tissue to be detected is performed as follows.
(a) The pulse laser light source 7 emits pulse laser, which is coupled by a coupling mirror and enters the excitation fiber 3 with the core diameter of 400 μm. In order to save space, the excitation optical fiber 3 and the collection optical fiber 4 are gathered or separated at the tail end of the handheld handle 5, the optical fibers are not affected with each other and are shaped outside the handheld handle 5 and the sleeve main body of the biopsy device.
(b) After the pulse laser irradiates the living body tissue 16 to be measured, fluorescence is excited, and the excited fluorescence is received and transmitted by the collection optical fiber 4. At the other end of the collection fiber 4, the fluorescent light beam is output and enters the spectroscopic system 8. The fluorescence entering the light splitting system 8 is collimated into parallel light beams by a collimating lens 10, the parallel light beams are divided into two paths after passing through a first dichroic beam splitter 11, wherein the wavelength range of the reflected light beams is 360 nm-430 nm, and the wavelength range of the transmitted light beams is 430 nm-700 nm; the light beam transmitted from the first spectroscope 11 passes through the second spectroscope 12 and then is divided into two paths, wherein the wavelength range of the reflected light beam is 430 nm-480 nm, and the wavelength range of the transmitted light beam is 480 nm-700 nm; the light beam transmitted from the second beam splitter 12 passes through the third beam splitter 13 and then is divided into two paths, wherein the wavelength range of the reflected light beam is 480 nm-550 nm, and the wavelength range of the transmitted light beam is 550 nm-700 nm; the light beam transmitted from the third beam splitter 13 is reflected by a mirror 14.
(c) The collected fluorescence information is transmitted to the computer and image display module 9 after signal conversion. The computer and image display module 9 processes the fluorescence signals varying with time at different central wavelengths to obtain fluorescence lifetime and fluorescence peak intensity information corresponding to each wavelength. Wherein the fluorescence lifetime corresponding to the central wavelength of 400nm is 10.5ns, and the fluorescence peak intensity is 5.6 muW; the fluorescence lifetime corresponding to the central wavelength of 450nm is 7.3ns, and the fluorescence peak intensity is 8.4 muW; the fluorescence lifetime corresponding to the central wavelength of 530nm is 13.3ns, and the fluorescence peak intensity is 4.5 muW; the fluorescence lifetime corresponding to a central wavelength of 580nm is 6.7ns, and the fluorescence peak intensity is 9.8. mu.W. Further, the relative fluorescence intensity spectrums of the living body lesion tissues to be detected under different wavelengths of 400nm, 450nm, 530nm, 580nm and the like are 0.57:0.86:0.46: 1.
(d) According to the fluorescence lifetime of the fluorescence of the normal living tissue under each wavelength, the fluorescence lifetime corresponding to the wavelength of 400nm is between 9ns and 13ns, the fluorescence lifetime corresponding to the wavelength of 450nm is between 10ns and 14ns, the fluorescence lifetime corresponding to the wavelength of 530nm is between 12ns and 15ns, and the fluorescence lifetime corresponding to the wavelength of 580nm is between 11ns and 14 ns; fluorescence lifetime of tumor biopsy fluorescence at each wavelength: the fluorescence lifetime corresponding to the wavelength of 400nm is between 8ns and 11ns, the fluorescence lifetime corresponding to the wavelength of 450nm is between 6ns and 8ns, the fluorescence lifetime corresponding to the wavelength of 530nm is between 13ns and 16ns, and the fluorescence lifetime corresponding to the wavelength of 580nm is between 5ns and 7 ns; it is possible to obtain the fluorescence lifetime information excited from the living tissue 16 to be measured which completely matches the fluorescence lifetime of the tumor living tissue, and thus it is possible to judge that the living tissue 16 to be measured is the tumor living tissue. Meanwhile, according to the fact that the peak fluorescence spectrum of the normal living tissue in the four-wavelength fluorescence information is 530nm, and the peak fluorescence spectrum of the tumor living tissue in the four-wavelength fluorescence information is 580nm, it can be further determined that the living tissue 16 to be measured is the tumor living tissue. And c, moving the fluorescence lifetime detection device based on optical fiber transmission to detect the position, and repeating the steps a to c. When the living tissue 16 to be measured is judged as a tumor living tissue, marking as a potential puncture point; if the living tissue 16 to be measured is judged to be a normal living tissue, the tissue is discarded as a potential puncture point.
(e) Before triggering biopsy, the patient should be confirmed to have no puncture biopsy contraindication, the operation should be terminated in time for the patient with severe bleeding tendency and extreme exhaustion which cannot tolerate the operation, the puncture points closely related to the large blood vessels and nerves are excluded, the puncture points possibly causing other serious complications are also excluded, and the optimal puncture point and puncture path are selected from the marked potential puncture points. The position, angle and direction of the biopsy needle 1 are adjusted by holding the handle 5, and then the biopsy button 6 is pressed by hand, the needle core 18 of the biopsy needle 1 rapidly enters the tumor living tissue through the coaxial catheter 19, and the tumor tissue is cut in the sample groove 20. After the puncture is completed, the biopsy device is pulled out along the original puncture path, and the tumor tissue sample is taken out from the sample groove 20 and placed into a sample bottle. The materials can be repeatedly taken along the original puncture point by adjusting different angles and directions so as to ensure the full sampling. After the operation is finished, the puncture point is pressed for 15 minutes or an elastic bandage is used when necessary, and the postoperative care is taken to the patients whether bleeding and nerve injury exist or not and operation-related complications occur, and if so, the patients are treated in time.
(f) In addition, fluid can be selectively injected into the longitudinally extending fluid passage 21 and connected to the fluid passage connector 17 at the operating end of the handle 5, at least one fluid outlet or return passage, as desired, and the fluid passage can be blocked from air ingress immediately after each aspiration.
Throughout the procedure, if all or part of the instrument is disposable, the disposable element is discarded, and if the device is not disposable, it is disassembled, cleaned, sterilized and repackaged in a conventional manner.
Example 2
When the device is used for the cavity access needle biopsy, different from the embodiment 1, the length, the diameter, the shape and the material of the needle biopsy device based on the optical fiber transmission type fluorescence life guidance are customized according to the actual requirement, and the device is used for the channels of the larynx, the trachea, the bronchus, the esophagus, the gastrointestinal tract, the vagina uterus and the like of the human. In this case, the front end of the needle biopsy device based on the fiber-optic transmission fluorescence lifetime guidance is mostly designed as a long and flexible fiber optic shaft, and only the working area near the distal end is designed as a small rigid section for facilitating biopsy and for accommodating a blade of biopsy tissue. When the cavity access needle biopsy is carried out, the area range of the needle biopsy can be increased by using a mechanical control device and a driving device, and the mechanical complexity is increased in a noted manner.
Example 3
When used for puncture biopsy of harder tissue, different from embodiment 1, the optical fibers outside the main body of the coaxial catheter 19 of the biopsy device are all placed in a hard sleeve so as to penetrate the harder tissue, the head ends of the optical fibers are made of transparent hard glass to keep the light path unobstructed, and the longitudinal fluid channel 21 is designed to ensure that the head ends of the optical fibers are not shielded.
Example 4
Different from embodiment 1, as shown in fig. 3, the light splitting system is composed of a collimating lens 10 and a grating 15, the collimating lens 10 collimates the light beam emitted from the collecting fiber 4, and the grating 15 splits the collimated light beam to obtain four paths of fluorescent light beams.
While the invention has been described in connection with specific embodiments thereof, it will be understood that these should not be construed as limiting the scope of the invention, which is defined in the appended claims, any modifications to which this invention pertains being applicable being within the scope of the invention defined in the following claims.
Claims (10)
1. The biopsy aspiration method based on the optical fiber transmission type fluorescence life guidance is characterized in that:
(a) the optical fiber transmission type fluorescence life detection device transmits pulse laser through an excitation optical fiber and irradiates the living tissue to be detected;
(b) the fluorescence signal excited from the living tissue to be detected is received by the collecting optical fiber of the optical fiber transmission type fluorescence lifetime detection device and enters the fluorescence detection module to obtain fluorescence lifetime information and fluorescence spectrum information of the living tissue to be detected;
(c) identifying whether the living tissue to be detected is a pathological tissue or not according to the detected fluorescence lifetime information and fluorescence spectrum information;
(d) moving the optical fiber transmission type fluorescence life detection device to detect the position, repeating the steps a to c, and detecting the position of the pathological change tissue;
(e) and performing puncture biopsy according to the detected position of the pathological tissue, selecting a proper puncture point, adjusting the position, the angle and the direction of the biopsy device, triggering the biopsy device to puncture, and taking out the pathological tissue.
2. The needle biopsy method based on the fiber transmission type fluorescence lifetime guidance according to claim 1, wherein: when the optical fiber transmission type fluorescence life detection device detects whether the tissue to be detected is pathological change tissue, the steps are as follows:
(a) transmitting and irradiating pulse laser on the living body tissue to be detected through an excitation optical fiber, wherein one end of the excitation optical fiber receives the pulse laser coupled by a pulse laser light source, and the other end of the excitation optical fiber guides the pulse laser out and irradiates the living body tissue to be detected;
(b) transmitting fluorescence excited from living body tissue to be measured by collecting optical fiber, dividing into N paths by using light splitting system, filtering each divided path of fluorescence by band pass filters with different central wavelengths, wherein the central wavelength corresponding to each band pass filter is lambdanBand pass width of δ λnWherein N is 1,2, …, N;
(c) measuring the fluorescence light intensity information which changes along with time under the corresponding wavelength and is filtered by each band-pass filter by using N light intensity detectors respectively, and obtaining the fluorescence service life under the corresponding wavelength and a relative fluorescence intensity spectrum between different wavelengths after data analysis;
(d) and identifying whether the living tissue to be detected is the pathological change tissue or not by the fluorescence lifetime of the living tissue to be detected under different wavelengths and the relative fluorescence intensity spectrum information among different wavelengths.
3. The needle biopsy method based on the fiber transmission type fluorescence lifetime guidance according to claim 1 or 2, characterized in that: when identifying whether the living tissue to be detected is a pathological tissue: and identifying whether the living tissue to be detected is a pathological change tissue or not through the fluorescence life of the living tissue to be detected under different wavelengths.
4. The needle biopsy method based on the fiber transmission type fluorescence lifetime guidance according to claim 2, wherein: replacing the excitation and collection fibers with fiber bundles: one optical fiber in the optical fiber bundle is used as an excitation optical fiber to transmit pulse laser, and the rest M optical fibers in the optical fiber bundle are used as collecting optical fibers to collect fluorescence excited from the substance to be detected; and the M optical fibers are transmitted and then combined at the tail ends, and the collected fluorescence is combined and then transmitted to the light splitting system.
5. The needle biopsy method based on the fiber transmission type fluorescence lifetime guidance according to claim 2, wherein: replacing the excitation fiber, collection fiber and splitting system with a fiber optic bundle: one optical fiber in the optical fiber bundle is used as an excitation optical fiber to transmit pulse laser, the rest N optical fibers in the optical fiber bundle are used as collecting optical fibers to collect fluorescence excited from a substance to be detected, the N optical fibers are separated after transmission to obtain N paths of collected fluorescence, and then the N paths of collected fluorescence are filtered by N band-pass filters with different central wavelengths.
6. The needle biopsy method based on the fiber transmission type fluorescence lifetime guidance according to claim 1, wherein: the biopsy needle method adjusts the type, length, diameter, operation mode and material of the biopsy needle device according to the classification of tissues, biopsy parts, the size of a sample obtained from a material and the biopsy sampling mode in the biopsy needle process, and provides a longitudinally extending fluid channel connection by means of a joint at the operation end of a handheld handle.
7. Puncture biopsy device based on guide of optic fibre transmission type fluorescence life-span, its characterized in that: the device comprises an optical fiber transmission type fluorescence life detection device and a biopsy device, wherein the optical fiber transmission type fluorescence life detection device comprises a pulse laser, an excitation optical fiber, a collection optical fiber and a fluorescence detection module; the biopsy device includes a handle and a biopsy needle comprised of a core and a coaxial catheter.
8. The fiber optic transmission-based fluorescence lifetime-guided needle biopsy device of claim 7, wherein: the fluorescence detection module of the optical fiber transmission type fluorescence life detection device comprises a light splitting system, N band-pass filters, N light intensity detectors, a signal collector and a computer; the fluorescence signal entering the fluorescence detection module is divided into N paths by a light splitting system, N band-pass filters respectively filter N paths of fluorescence, N light intensity detectors respectively detect fluorescence light intensity information which is filtered by each band-pass filter and changes along with time under corresponding central wavelength, the signal collector converts the fluorescence light intensity information detected by each light intensity detector and transmits the converted fluorescence light intensity information to the computer, and the computer analyzes the converted fluorescence light intensity information to obtain the fluorescence life and relative fluorescence intensity spectrum between different wavelengths.
9. The fiber optic transmission-based fluorescence lifetime-guided needle biopsy device of claim 7, wherein: replacing the excitation and collection fibers with fiber bundles: one optical fiber in the optical fiber bundle is used as an excitation optical fiber to transmit pulse laser, and the rest M optical fibers in the optical fiber bundle are used as collection optical fibers to collect fluorescence excited from a substance to be detected; the M optical fibers are transmitted and then merged at the tail end, and the collected fluorescence is merged and then transmitted to the fluorescence detection module.
10. The fiber optic transmission-based fluorescence lifetime-guided needle biopsy device of claim 7, wherein: replacing the excitation fiber, collection fiber and splitting system with a fiber optic bundle: one optical fiber in the optical fiber bundle is used as an excitation optical fiber to transmit pulse laser, the rest N optical fibers in the optical fiber bundle are used as collecting optical fibers to collect fluorescence excited from a substance to be detected, the N optical fibers are separated after transmission to obtain N paths of collected fluorescence, and then the N paths of collected fluorescence are filtered by N band-pass filters with different central wavelengths.
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