CN201042433Y

CN201042433Y - Living body tissue optical detector

Info

Publication number: CN201042433Y
Application number: CNU2007200371882U
Authority: CN
Inventors: 马剑文; 王成; 徐安成; 马博平
Original assignee: Individual
Current assignee: Individual
Priority date: 2007-04-28
Filing date: 2007-04-28
Publication date: 2008-04-02
Anticipated expiration: 2017-04-28

Abstract

The utility model relates to a biopsy device. The biopsy device includes a light source emitting unit, an optical fiber probe, a spectral analysis and data processor unit, an emitting optical fiber, a receiving fiber and an optical switch. The optical fiber probe is a chromatographic surface contact nondestructive optical fiber probe, distances between each optical fiber of emitting optical fibers and receiving optical fibers in the optical fiber probe are all or partially different, and the number of the different distances is larger than or equal to 2. Application method of the biopsy device in the utility model includes comparing light intensity information of each wavelength in the obtained diffuse reflection light of biopsy with light intensity standard to obtain a ratio sequence which is used as diffuse reflection spectrum signal, and controlling state of the switch to detect state of living tissue at different depth, thereby obtaining detection result. The utility model can perform chromatographic optical detection with high detection precision.

Description

The biological tissue optical detection apparatus

Technical field

This utility model relates to a kind of device that biological tissue is detected.Through detection resulting result can be for the medical worker to biological tissue in the optical detection of the tumor of carrying out etc.

Background technology

To human body particularly the tissue in the tissue carry out the noinvasive triage and survey, harmless, the quick diagnosis of the important meaning, particularly various tumors arranged on clinical medicine, make a definite diagnosis and to improve cancer patient's survival rate all significant early stage.

Mostly the doctor is by virtue of experience the diagnosis of cancer and the inspection of biological tissue section is realized having certain subjectivity and blindness, cause early lesion to be failed to pinpoint a disease in diagnosis easily or mistaken diagnosis.

Laser medicine is the new branch of science of modern medicine, and it combines the new and high technology in fields such as laser, spectroscopy, fibre optics, optical information processing, tissue optical property together, is applied to the diagnosis and the treatment of each disease of human body.The method of noinvasive or Wicresoft's live body detection mainly is the method that imaging detects at present, as: X-ray, CT, MIR, PET and CTPET etc. utilize radiation source and high-intensity magnetic field imaging mode.Research to optical imagery in recent years deepens continuously, and especially the optical imaging method to diagnosis of breast disease constantly occurs, as infrared thermal imaging, near infrared imaging.Infrared thermal imaging is that the characteristic of utilizing the body temperature of tumor locus to be higher than normal structure is carried out the Temperature Distribution imaging, because of its accuracy is not high, has not re-used substantially in recent years; The principle of near infrared imaging is to utilize heterogeneity in the tissue to the absorption characteristic of light, especially in the blood of tumor canceration the increase of deoxyhemoglobin and total blood volume increase to foundation, carry out the imaging of tumor locus, but it passes through transmission mode mostly, image resolution ratio is low, can only carry out imaging to external tissue.The diagnosis shortcoming of described CT, MRI imaging is all to have high-energy radiation, and human body is had certain injury, and detects the cost height, and ordinary people is difficult to bear this testing cost.

OCT is a kind of new optical detective technology, it also is to have utilized optical coherence that tissue is carried out the characteristic that tissue is analyzed in imaging, its shortcoming is the equipment operation complexity, cost an arm and a leg, date processing difficulty height, and major function is tomography, but the resolution of imaging is still waiting to improve.It is as the equipment that optical field is emerging, and cost performance is not high, mainly still will lean on doctor's clinical experience.

The diagnosing tumor method is also comprised the photodynamics diagnostic method, and it utilizes histofluorescence or external source fluorescence to carry out diagnosing tumor, is a kind of auxiliary diagnosis means behind the positioning tumor position, for autofluorescence, has only and could diagnose after producing fluorescence; And after the injection of external source fluorescent agent, patient needs lucifuge about one week, has brought inconvenience to the patient, and fluorescent agent is harmful mostly.

For example: Chinese patent literature Granted publication CN1256918 and Chinese patent literature publication number CN1418601 all disclose described photodynamics diagnostic method.

International Publication No. WO2005/092194 disclose a kind of in real time noninvasive based on the metamorphosis of elastic scattering spectrographic detection cancerous cell at body canceration diagnostic method, specifically disclose the equipment and the method for record back-scattering light in a kind of small angle range, small angle range is by the numerical aperture restriction of single fiber probe.Its used optic probe had both transmitted incident illumination, also transmitted the scattered light of collecting from tissue surface.The weak point of this technical scheme is: this scheme is only considered the scattering properties of light on histiocyte, does not consider that chromophore is to the absorption characteristic of light in the organizer, and this causes the reliability of its measurement result not high, causes easily failing to pinpoint a disease in diagnosis or mistaken diagnosis.

American documentation literature publication number US2006/0173352A1 discloses the method for the radiationless spectrographic detection pathological tissues of a kind of usefulness body, the incident structure that contains certain spectral region and the structure of detection from light this organizer transmission or reflection that have wherein comprised the selecteed organizer of irradiation mammal are to obtain transillumination or catoptrical spectrum.This spectrum has been represented one or more basic spectral components, the subscale mark coefficient of an error term and each basic spectral component.The subscale mark coefficient is calculating with the minimum error item.The subscale mark coefficient of each basic spectral component is revised with the pre-selected characteristics of the tissue of the known susceptibility to disease of having selected.The weak point of this scheme is: it mainly is to detect the characteristic that the mode of transmitted spectrum obtains to organize, detect similar with X-ray mammary gland, this mode will be compressed to mammary gland tissue and to a certain degree could begin to detect, bring certain misery to patient, and can not survey and diagnose in-vivo tissue.

U.S. Patent number US6091984A1 and International Patent Application WO 9214399 all disclose the system and method that a kind of method detection of biological of utilizing optical scattering is organized physical characteristic.This system uses fibre system to carry and collects from the next light of domain of dependence one or more physical characteristics of detection surface layer.This system comprises wideband light source, and fiber device is carried and/or collected from the light of organizing, the scattered light of monitor system monitoring from organizing, and the spectrum that computer analysis and storage monitor, and show test results.The light that lens are used for coming from light source is connected to the launching fiber of probe.Filter disc and lens combination are used for collection light is delivered to spectrograph effectively.Control light source with the controller that clock is connected with pulser.The weak point of this system is: the computer in this system is when work, the light that utilizes the monitor system to receive is analyzed the physical characteristic of the surface layer of surveying, simultaneously with the variation of light intensity as the light source feedback signal, stability by the output of controller control light source, this feedback control more complicated, signal of telecommunication control often feedback lags behind seriously, causes light source output unstable, and then causes the detection error of this system bigger.In addition, this system can't compare the light intensity of each wavelength of light source output and the light intensity that diffuses from tissue surface, the diffuse reflection spectrum deviation that can be used for of causing that computer obtains analyzed biological tissue's physical characteristic is big, even can't obtain available diffuse reflection spectrum, so the reliability of this system is not high.

Above-mentioned the deficiencies in the prior art part also is, only can carry out optical detection to the biological tissue of the single degree of depth, and practical situation is; the biological tissue of pathological changes has the certain horizontal area and the vertical degree of depth, through the biological tissue to the single degree of depth is carried out the situation that optical detection obviously can not accurately detect the biological tissue neoplastic lesion.

The utility model content

The technical problems to be solved in the utility model provides that a kind of can to carry out optical detection to the biological tissue of different depth be the higher biological tissue optical detection apparatus of chromatography optical detection and accuracy of detection.

For solving the problems of the technologies described above, realize that the technical scheme that a kind of biological tissue optical detection apparatus is provided in this utility model purpose is: checkout gear of the present utility model has the main apparatus main body of detection, detects main apparatus main body and comprises: light emitted unit, fibre-optical probe, spectrum analysis and data processing unit, launching fiber and reception optical fiber; The unitary light source outfan of preceding termination light emitted of launching fiber receives the back termination spectrum analysis of optical fiber and the reflected light input of data processing unit; Fibre-optical probe comprises the rear end of launching fiber and receives the front end of optical fiber; The light that the unitary light source outfan of light emitted is sent when being to use is through the outfan of launching fiber by fibre-optical probe directive outside, and the light that the reflected light input of spectrum analysis and data processing unit is received when being to use is from port outside and that this light transmits through reception optical fiber; Can handle the reflection ray that is received when spectrum analysis and data processing unit are to use and draw the intensity signal of wherein each wavelength and then obtain the device of reflected light spectral signal; It is characterized in that:

Detect main apparatus main body and also comprise photoswitch; Fibre-optical probe is the harmless probe of surface contact; Photoswitch has light input and two optical ports of light output end, and in these two optical ports: a port is the single channel end, and another port is the branch terminal that has 2 shunt at least; The then corresponding still chromatography type fibre-optical probe of fibre-optical probe also be that the distance between the every optical fiber in every optical fiber and the reception optical fiber in the launching fiber in the fibre-optical probe is all or part of inequality, and the quantity of these distances inequality is more than or equal to 2; Photoswitch by its optical port be connected on the unitary light source outfan of light emitted to the light path between the rear end of launching fiber or/and be connected on receive optical fiber front end to the light path between the reflected light input of spectrum analysis and data processing unit, also be that photoswitch is connected on the unitary light source outfan of light emitted to the light path between the reflected light input of spectrum analysis and data processing unit; When photoswitch is to use because of anastomosing shunt difference corresponding optical fiber in the launching fiber in the fibre-optical probe and the corresponding optical fiber that receives in the optical fiber are in the photoswitch that the turn-on and turn-off state is controlled.

Above-mentioned launching fiber is divided into leading portion and back segment or/and receive optical fiber, and the back segment of launching fiber is or/and the leading portion of reception optical fiber is the fibre bundle with at least 2 optical fiber; Wherein,

When photoswitch is connected on the light path of the unitary light source outfan of described light emitted between the rear end of launching fiber by its optical port, receiving optical fiber is single optical fiber, launching fiber is divided into leading portion and back segment, the leading portion of launching fiber has at least 1 optical fiber, and the back segment of launching fiber is the fibre bundle with at least 2 optical fiber; The unitary light source outfan of light emitted then links to each other with front end as the leading portion of the launching fiber of launching fiber front end, the single channel end of the back termination photoswitch of the leading portion of launching fiber, and the single channel end of this moment is then as the light input; Each of the branch terminal of photoswitch links to each other with the front end of a corresponding optical fiber in the back segment of launching fiber along separate routes, and branch terminal at this moment is then as light output end; Have nothing in common with each other as the rear end of each optical fiber in the back segment of the launching fiber of the rear end of launching fiber spacing with the front end that receives optical fiber;

When photoswitch is connected on the light path of front end between the reflected light input of spectrum analysis and data processing unit of described reception optical fiber by its optical port, launching fiber is single optical fiber, receive optical fiber and be divided into leading portion and back segment, the leading portion that receives optical fiber is the fibre bundle with at least 2 optical fiber, and the back segment that receives optical fiber has at least 1 optical fiber; Spacing as the rear end of the front end of each optical fiber in the leading portion of the reception optical fiber of the front end that receives optical fiber and launching fiber has nothing in common with each other; The rear end of each optical fiber in the leading portion of reception optical fiber links to each other with a corresponding shunt of the branch terminal of photoswitch, and the branch terminal of this moment is then as the light input; The single channel end of photoswitch links to each other with the front end of the back segment that receives optical fiber, and the single channel end of this moment is then as light output end; Rear end as the back segment of the reception optical fiber of the rear end that receives optical fiber then links to each other with the reflected light input of spectrum analysis and data processing unit;

When photoswitch is connected on the unitary light source outfan of light emitted to the light path between the rear end of launching fiber and when being connected on the light path of front end between the reflected light input of spectrum analysis and data processing unit that receives optical fiber by its optical port, the quantity of photoswitch is 2, and first photoswitch is connected on the unitary light source outfan of described light emitted to the light path between the rear end of launching fiber by its optical port, and second photoswitch is connected on the front end of described reception optical fiber to the light path between the reflected light input of spectrum analysis and data processing unit; Launching fiber is divided into leading portion and back segment, and the leading portion of launching fiber has at least 1 optical fiber, and the back segment of launching fiber is the fibre bundle with at least 2 optical fiber; The unitary light source outfan of light emitted then links to each other with front end as the leading portion of the launching fiber of launching fiber front end, the single channel end of back first photoswitch of termination of the leading portion of launching fiber, and the single channel end of this moment is then as the light input; Each of the branch terminal of first photoswitch links to each other with the front end of a corresponding optical fiber in the back segment of launching fiber along separate routes, and branch terminal at this moment is then as light output end; Receive optical fiber and also be divided into leading portion and back segment, the leading portion that receives optical fiber is the fibre bundle with at least 2 optical fiber, and the back segment that receives optical fiber has at least 1 optical fiber; Have nothing in common with each other as the rear end of each optical fiber in the back segment of the launching fiber of the rear end of launching fiber and spacing as the front end of each optical fiber in the leading portion of the reception optical fiber of the front end that receives optical fiber; The rear end of each optical fiber in the leading portion of reception optical fiber links to each other with a corresponding shunt of the branch terminal of second photoswitch, and the branch terminal of this moment is then as the light input; The single channel end of second photoswitch links to each other with the front end of the back segment that receives optical fiber, and the single channel end of this moment is then as light output end; Rear end as the back segment of the reception optical fiber of the rear end that receives optical fiber then links to each other with the reflected light input of spectrum analysis and data processing unit.

Above-mentioned light source incident unit sends the device that wave-length coverage is 400～1000 nanometer light when being to use, the light that sends when being to use specifically is the light of 2～4 wavelength of wavelength in 400～1000 nanometer wavelength range, or the waveband width of wavelength in 400～1000 nanometer wavelength range be the light of the continuous wavelength of 100～600 nanometers, or have successive white-light spectrum, its wave band is the light of visible light wave range and/or near infrared band.

Above-mentioned light emitted unit has light source, focuses on coupling device, light splitting piece, photoelectric switching circuit, drive circuit and power supply; Power supply links to each other with the power end of light source through control circuit; On the unitary light path of light emitted, be provided with light source successively, focus on coupling device, light splitting piece and photoelectric switching circuit; Behind the light line focus coupling device that sends from light source, a part of light passes light splitting piece and is coupled in the launching fiber, and another part light is incident upon the optical signal receiving terminal of photoelectric switching circuit after the surface reflectance of light splitting piece; The electrical signal of photoelectric switching circuit links to each other with the feedback signal input of drive circuit, with the intensity of stabilized light source output light.

Above-mentioned spectrum analysis and data processing unit also comprise a memorizer that stores each wavelength light strong basis standard of diffuse light; Then under the situation of reflection ray that when spectrum analysis and data processing unit use, is received as diffuse light, when also being to use, spectrum analysis and data processing unit can obtain the intensity signal of each wavelength in the diffuse light, intensity signal to this each wavelength is changed and the spectrum signal that obtains diffusing, the device of last this spectrum signal that diffuses of outwards output; Above-mentioned intensity signal conversion to each wavelength is meant: the light intensity benchmark with the respective wavelength in the intensity signal of this each wavelength and each wavelength light strong basis standard of being stored is compared the sequence that obtains a ratio, and this sequence of ratio values is handled the back as the spectrum signal or direct as the spectrum signal that diffuses that diffuses.

Above-mentioned spectrum analysis and data processing unit comprise fiber spectrometer, date processing and memory element and the display unit that is electrically connected successively; Fiber spectrometer has optical signal input and detection signal outfan, and the optical signal input of fiber spectrometer is the reflected light input of spectrum analysis and data processing unit; The memorizer that stores each wavelength light strong basis standard of diffuse light is arranged in date processing and the memory element; When being to use, fiber spectrometer can receive device from the light that receives optical fiber, can draw when fiber spectrometer also is to use with this reception light in the corresponding digital signal of light intensity of each wavelength, and then export the device of this digital signal by its detection signal outfan, and if this reception light is incident upon and can carries out irreflexive object to light to the outside for the light that this device sends, carry out diffuse-reflectance by this object again and when receiving optical fiber and transport to the light of fiber spectrometer, can draw when then fiber spectrometer is to use with this diffuse light in the corresponding digital signal of light intensity of each wavelength, and then export the device of this digital signal by its detection signal outfan; When being to use, date processing and memory element can receive the device of above-mentioned digital signal by its detection signal input; Can draw the device of the intensity signal of each wavelength in the corresponding light when date processing and memory element also are to use according to described digital signal, and date processing and memory element are changed and the device of the spectrum signal that obtains diffusing to the intensity signal of each wavelength still; Can receive the above-mentioned spectrum signal that diffuses when display unit is to use, again this signal be carried out device shown.

Checkout gear of the present utility model also comprises a memorizer that is used to store the spectra database of the same type biological tissue diffuse reflection spectrum of gathering in advance, and this memorizer is provided with separately or is arranged in spectrum analysis and the data processing unit; During spectrum analysis and data processing unit also are to use behind the spectrum signal that obtains diffusing again to described spectra database in each diffuse reflection spectrum of same type biological tissue carry out the similarity contrast and find out similar diffuse reflection spectrum among the data base, belong to the biological tissue of which kind of state and then draw comparison result by this similar diffuse reflection spectrum, the device of last this comparison result of outwards output.

Checkout gear of the present utility model also comprises the diffuse reflector that is independent of the main device of detection; The reflectance of this diffuse reflector is 12% to 30%, during being to use, this diffuse reflector will detect the parts of the light diffuse-reflectance back into optical fibers probe that the fibre-optical probe of main device sends, so that spectrum analysis and data processing unit carry out spectrum analysis to this diffuse light, draw each wavelength in the diffuse light of this moment intensity signal and as the light intensity benchmark of reflection ray.

Above-mentioned fibre-optical probe also has fixed block, and fixed block is provided with fixing hole, and the front end setting of each optical fiber of the rear end of each optical fiber of launching fiber and reception optical fiber also is fixed in the corresponding fixing hole of fixed block.

The utlity model has positive effect: the main device of detection in (1) this utility model device can carry out optical detection to the biological tissue of different depth; As long as photoswitch is connected on the unitary light source outfan of light emitted to the light path between the reflected light input of spectrum analysis and data processing unit, and is easy to install; Fibre-optical probe is the harmless probe of surface contact, directly contacts with the body surface of biological tissue to be measured during use to get final product, and human body is not had any damage.(2) photoswitch of this utility model device is suitable for the light of input single channel end is selected from each minute one of terminal output, or the light of selecting import one of described minute terminal exports from the single channel end, and is simple in structure, easy to install; Its light that sends was suitable for carrying out the biological tissue optical detection when (3) device of the present utility model used, and spectrum analysis is simple.(4) the light emitted cellular construction of this utility model device is simple and reliable, especially light splitting piece, photoelectric switching circuit and drive circuit are constituted the electro-optical feedback system, the output of monitoring light source in real time, so that light source output is stable, guaranteed the workability and the reliability of biological tissue tumor optical detection apparatus of the present utility model.Wherein, the reflectance of light splitting piece is 0.5% to 1.5%, and the resolving accuracy of photoelectric switching circuit is high more, and the reflectance of the light splitting piece that is adopted can be low more, and the light source control precision is just high more, and light source output is just stable more.(5) spectrum analysis of this utility model device and data processing unit are used for handling from the light that receives optical fiber and obtaining the reflected light spectral signal, and it is simple in structure, and is easy to use.(6) when being provided with the memorizer of the spectra database that is used to store the same type biological tissue diffuse reflection spectrum of gathering in advance in the device of the present utility model, each diffuse reflection spectrum that biological tissue can be diffused among spectrum signal and the data base carries out the similarity contrast, and obtain comparison result, last this comparison result of outwards output, easy to use.(7) in the method for work of the present utility model, before biological tissue is detected, the employing reflectance is 12% to 30% diffuse reflector, by the unitary output light of light emitted, through this diffuse reflector reflection, after receiving this diffuse light by spectrum analysis and data processing unit again it is handled, obtain light that this device sends after diffuse-reflectance in the intensity signal of each wavelength, and with the light intensity benchmark of this intensity signal as emission light.And different biological tissues for the diffuse reflectance of different wave length light usually about 2%～18%, be 12%～30% the resulting light intensity benchmark of diffuse reflector so adopt diffuse reflectance, help improving the reliability and the precision thereof of optical detection.Under the guaranteed prerequisite of spectrum analysis precision of spectrum analysis and data processing unit, the reflectance of the diffuse reflector that is adopted is low more, and certainty of measurement is then high more.(8) checkout gear of the present utility model can be applicable at body surface detection mammary gland diffuse reflection spectrum, has reduced in the prior art radiation hazradial bundle to patient.Fibre-optical probe in the checkout gear of the present utility model also can enter in the human body, carries out real-time, dynamic, noninvasive detection, and the probe profile of this moment can adopt " L " shape, so that inner chamber mucomembranous surfaces such as stomach, intestinal are detected.(9) comprise display unit and special memorizer is set and during corresponding program, display unit can intuitively show and corresponding pathology picture of the spectrum information that diffuses of biological tissue and doctor's advice when the spectrum analysis in the checkout gear of the present utility model and data processing unit; Also can show the content value of major parameter relevant such as blood volume, blood oxygen amount, water and fat, and can compare the testing result in a period of time, analyze the variation tendency of this biological tissue, reference is provided for doctor's diagnosis and treatment with tumor.(10) there is more composition in the tissue to visible light wave range and the absorption of near infrared light wave band,, deoxyhemoglobin white, fat, water etc. as the Oxygenated blood red eggs, and in the described cell component in the pathological tissues and structure and the normal structure many variations being arranged, these variations can obtain reflection on diffuse reflection spectrum; Biological tissue optical detection apparatus of the present utility model utilizes improper biological tissue with normal biological tissue the different absorption characteristics of light to be carried out optical detection just; draw the situation of biological tissue by analysis, have fast, in real time, accurately and reliably and painless effect diffuse reflection spectrum.

Description of drawings

Fig. 1 is a kind of structural representation of biological tissue optical detection apparatus of the present utility model;

Fig. 2 is the unitary structural representation of light emitted among Fig. 1;

Fig. 3 is the circuit block diagram of spectrum analysis and data processing unit among Fig. 1;

Fig. 4-1 is a kind of structural representation of fibre-optical probe among Fig. 1, photoswitch, launching fiber and reception optical fiber mutual relation; Fig. 4-2 is the another kind of structural representation of fibre-optical probe among Fig. 7, photoswitch, launching fiber and reception optical fiber mutual relation; Fig. 4-3 is the third structural representation of fibre-optical probe among Fig. 8, photoswitch, launching fiber and reception optical fiber mutual relation;

Fig. 5-1 is the structural representation that photoswitch links to each other with the optical fiber of the front and back section of launching fiber among Fig. 4-1; Fig. 5-2 is the structural representation that photoswitch links to each other with the optical fiber of the front and back section that receives optical fiber among Fig. 4-2; Fig. 5-3 is a kind of structural representation that photoswitch links to each other with the optical fiber of the front and back section of launching fiber among Fig. 4-3 structure and photoswitch link to each other with the optical fiber of the front and back section of reception optical fiber; Fig. 5-4 is the another kind of structural representation that photoswitch links to each other with the optical fiber of the front and back section of launching fiber among Fig. 4-3 structure and photoswitch link to each other with the optical fiber of the front and back section of reception optical fiber;

Fig. 6-1 is the structural representation of 6 kinds of fibre-optical probes of the present utility model to Fig. 6-6;

Fig. 7 is the another kind of structural representation of biological tissue optical detection apparatus of the present utility model;

Fig. 8 is the third structural representation of biological tissue optical detection apparatus of the present utility model;

Fig. 9 is the 4th a kind of structural representation of biological tissue optical detection apparatus of the present utility model;

Figure 10 is the FB(flow block) of the method for work of biological tissue optical detection apparatus of the present utility model;

Figure 11 judges the course block diagram of breast biological tissue for which kind of abnormal state in this utility model application examples 1.

Figure 12 is the diffuse reflection spectrum sketch map of normal gastric mucosa surface in this utility model application examples 2;

Figure 13 is the diffuse reflection spectrum sketch map of gastric cancer or adenocarcinoma mucomembranous surface in this utility model application examples 2;

Figure 14 is the diffuse reflection spectrum sketch map of normal esophagus in this utility model application examples 3;

Figure 15 is the diffuse reflection spectrum sketch map of canceration esophagus in this utility model application examples 3.

The specific embodiment

(embodiment 1)

See Fig. 1, the biological tissue optical detection apparatus of present embodiment has and detects main device and be independent of the diffuse reflector 6 that detects main device.Detect main device and comprise the main apparatus main body of detection; Detect main apparatus main body and have light emitted unit 1, fibre-optical probe 2, spectrum analysis and data processing unit 3, launching fiber 4, reception optical fiber 5 and photoswitch 7.

Light emitted unit 1 send when being to use the device that wave-length coverage is 400～1000 nanometer light (present embodiment emit beam when using have successive white-light spectrum, its wave band is visible light wave range and near infrared band, also can select to send the light emitted unit 1 of continuous visible light wave range, or the light emitted unit of selecting to send continuous near infrared band 1 is replaced); The light that the light source outfan of light emitted unit 1 is sent when being to use is through the outfan of launching fiber 4 by fibre-optical probe 2 directive outsides.

See Fig. 2, light emitted unit 1 has light source 11, focuses on coupling device 12, light splitting piece 13, photoelectric switching circuit 15, drive circuit 16 and power supply 17.Power supply 17 links to each other through the power end of control circuit 16 with light source 11; On the light path of light emitted unit 1, be provided with light source 11 successively, focus on coupling device 12, light splitting piece 13 and photoelectric switching circuit 15; Behind the light line focus coupling device 12 that sends from light source 11, a part of light passes light splitting piece 13 and is coupled in the launching fiber 4, and another part light is incident upon the optical signal receiving terminal of photoelectric switching circuit 15 after the surface reflectance of light splitting piece 13; The electrical signal of photoelectric switching circuit 15 links to each other with the feedback signal input of drive circuit 16, with the intensity of stabilized light source 11 output light.

Still see Fig. 1, in order to improve the efficiency of transmission of system, launching fiber 4 and reception optical fiber 5 adopt liquid-core optical fibres, also can adopt silica fibre to replace.Because the two ends of these optical fiber are branches of unmatched back, therefore, in use, arbitrary end of these optical fiber can be used as front end, and the remaining other end is then as the rear end.For launching fiber 4 and reception optical fiber 5, if these optical fiber are used for linking to each other with light emitted unit 1, then use as launching fiber 4, if these optical fiber are used for linking to each other with spectrum analysis and data processing unit 3, then use as reception optical fiber 5.The optional scope of the diameter of optical fiber is 0.1～2 millimeter millimeter, and the fibre diameter of present embodiment is 0.6 millimeter.

See Fig. 4-1 and Fig. 5-1, when photoswitch 7 is to use because of anastomosing shunt difference corresponding optical fiber in the launching fiber 4 in the fibre-optical probe 2 and the corresponding optical fiber that receives in the optical fiber 5 are in the optics that the turn-on and turn-off state is controlled.The quantity of the photoswitch 7 in the present embodiment is 1.This photoswitch 7 adopts 1 * 4 multimode optical switching of Shanghai Xiang light optical communication company.Photoswitch 7 is optic two terminal device, and the control of photoswitch 7 realizes by the control circuit that matches, and this optical switch control circuit can also can be controlled by circuit with manually control, photoswitch 7 is controlled by manually coming in the present embodiment; Photoswitch 7 has light input and two optical ports of light output end, and in these two optical ports: a port is a single channel end 71, this single channel end 71 has an optical fiber interface, another port of photoswitch 7 is the branch terminal 72 that has 2 shunt at least, and the branch terminal 72 of the photoswitch 7 of present embodiment has 4 optical fiber interfaces.

See Fig. 1, Fig. 4-1 and Fig. 5-1, in the present embodiment, launching fiber 4 is divided into two sections of front and back, the leading portion 41 of launching fiber 4 has only an optical fiber, the back segment 42 of launching fiber 4 (can be any probable value greater than 3 for the fibre bundle with 3 optical fiber in other embodiments, as 6,10,20 etc., also can be 2, the shunt quantity of the branch terminal 72 of corresponding photoswitch 7 then is not less than the radical of optical fiber of the back segment 42 of launching fiber 4); Receiving optical fiber 5 is single optical fiber.

Still see Fig. 1, Fig. 4-1 and Fig. 5-1, the front end of the leading portion 41 of launching fiber 4 is as the light source outfan of the preceding termination light emitted unit 1 of launching fiber 4, the optical fiber interface of the single channel end 71 of the back termination photoswitch 7 of the optical fiber of the leading portion 41 of launching fiber 4; At this moment, 71 at the single channel end of photoswitch 7 uses as the light input, its minute terminal then use as light output end.Each of the branch terminal 72 of photoswitch 7 optical fiber interface along separate routes is connected with the front end of a corresponding optical fiber of the back segment 42 of launching fiber 4, thereby make light source outfan that photoswitch 7 is connected on light emitted unit 1 by its optical port to as on the light path between the rear end of each optical fiber in the back segment 42 of the launching fiber 4 of the rear end of launching fiber 4, even also photoswitch 7 is connected on the light source outfan of light emitted unit 1 to the light path between the reflected light input of spectrum analysis and data processing unit 3.Receive the back termination spectrum analysis of optical fiber 5 and the reflected light input of data processing unit 3.

Fig. 4-1 and Fig. 6-1, fibre-optical probe 2 comprise launching fiber 4 back segment 42 each optical fiber the rear end, receive front end, shell 21 and the medical plastic fixed block 22 of optical fiber 5.Fixed block 22 is provided with 4 fixing holes.When making fibre-optical probe 2, the rear end of each optical fiber of the back segment 42 of launching fiber 4 and the front end that receives optical fiber 5 are penetrated in the corresponding fixing hole of fixed block 22, stick with glue agent optical fiber and fixed block 22 are fixedly bonded together, and the end face of end face and the front end that receives optical fiber 5 of rear end of each optical fiber of the back segment 42 of launching fiber 4 is positioned on the same plane; Then the package cover made from medical stainless steel as shell 21 fixed covers on fixed block 22; The length of described package cover 21 is about 25mm.Fibre-optical probe 2 is the chromatography type fibre-optical probe, be the spacing (all being as the criterion) inequality between the rear end face of every optical fiber in the back segment 42 of the launching fiber 4 in the fibre-optical probe 2 and the front end face that receives optical fiber 5, and the quantity of these distances inequality equal 3 with the centre distance between optical fiber and the optical fiber; Wherein, the distance between the adjacent fiber of the back segment 42 of launching fiber 4 is 5mm, and each root optical fiber on left side, centre and right side in figure of the back segment 42 of launching fiber 4 and the distance that receives between the optical fiber 5 are followed successively by 25mm, 20mm, 15mm; This set can make fibre-optical probe 2 in use can receive survey diffuse light on the different aspects in the biological tissue, be beneficial to this checkout gear the biological tissue at subcutaneous 4 to 5cm places carried out optical detection.

Still see Fig. 4-1 and Fig. 6-1, fibre-optical probe 2 is the harmless probes of surface contact, can directly contact with external surface, perhaps contacts with the body inner surface, to detect the situation of body surface undertissue.Outside launching fiber back segment 42 and reception optical fiber 5, be with medical grade rubber flexible pipe 8.

See Fig. 3, can handle and draw the intensity signal of wherein each wavelength and then obtain the device of reflected light spectral signal the reflection ray that is received when spectrum analysis and data processing unit 3 are to use, the light that the reflected light input of spectrum analysis and data processing unit 3 is received when being to use from outside and this light through receiving the ports that optical fiber 5 transmits.Spectrum analysis and data processing unit 3 comprise fiber spectrometer 31, date processing and memory element 32 and the display unit 33 that is electrically connected successively; Spectrum analysis and data processing unit 3 also comprise a memorizer that stores each wavelength light strong basis standard of diffuse light; Under the situation of reflection ray that when spectrum analysis and data processing unit 3 use, is received as the biological tissue diffuse light, spectrum analysis and data processing unit 3 can obtain the intensity signal of each wavelength in the diffuse light when also being to use, intensity signal to this each wavelength is changed and the spectrum signal that obtains diffusing, the device of last this spectrum signal that diffuses of outwards output; Above-mentioned intensity signal conversion to each wavelength is meant: the light intensity benchmark with the respective wavelength in the intensity signal of this each wavelength and each wavelength light strong basis standard of being stored is compared the sequence that obtains a ratio, and this sequence of ratio values is handled the back as spectrum signal or the direct spectrum signal that diffuses as biological tissue of diffusing.

Above-mentioned fiber spectrometer 31 has optical signal input and detection signal outfan, and the optical signal input of fiber spectrometer 31 is the reflected light input of spectrum analysis and data processing unit 3; The memorizer that stores each wavelength light strong basis standard of diffuse light is arranged in date processing and the memory element 32.When being to use, fiber spectrometer 31 can receive device from the light that receives optical fiber 5, can draw when fiber spectrometer 31 also is to use with this reception light in the corresponding digital signal of light intensity of each wavelength, and then export the device of this digital signal by its detection signal outfan, and if this reception light is incident upon and can carries out irreflexive object to light to the outside for the light that this device sends, carry out diffuse-reflectance by this object again and when receiving optical fiber 5 and transport to the light of fiber spectrometer 31, can draw when then fiber spectrometer 31 is to use with this diffuse light in the corresponding digital signal of light intensity of each wavelength, and then export the device of this digital signal by its detection signal outfan; When being to use, date processing and memory element 32 can receive the device of above-mentioned digital signal by its detection signal input; Can draw the device of the intensity signal of each wavelength in the corresponding light when date processing and memory element 32 also are to use according to described digital signal, and date processing and memory element 32 changed and the device of the spectrum signal that obtains diffusing to the intensity signal of each wavelength still; Can receive the above-mentioned spectrum signal that diffuses when display unit 33 is to use, again this signal be carried out device shown.

The AvaSpec-2048 FT or the USB2 type CCD spectrogrph of the Dutch Avantes of fiber spectrometer 31 employings company, this fiber spectrometer has comprised grating splitting system and other necessary optical element, assembly and array CCD detector.

Still see Fig. 3, date processing and memory element 32 are PC or embedded system; Fiber spectrometer 31 links to each other by the usb data line with date processing and memory element 32.Display unit 33 is a liquid crystal display.

Still see Fig. 1, be independent of the diffuse reflector 6 that is used to obtain the optical signal benchmark that detects main device, its reflectance is 20%.In the use, to diffuse reflector 6 emission light, the diffuse light of diffuse reflector 6 is sent into the reflected light input of spectrum analysis and data processing unit 3 through receiving optical fiber 5 by fibre-optical probe 2; 3 pairs of these diffuse lights of spectrum analysis and data processing unit carry out spectrum analysis, draw each wavelength in the diffuse light of this moment intensity signal and as the light intensity benchmark of reflection ray.

See Figure 10, the method for work of above-mentioned detection device comprises the steps:

1. obtain the light intensity benchmark., receive simultaneously from the diffuse light of diffuse reflector 6 and through receiving optical fiber 5 and send into the reflected light input of spectrum analysis and data processing unit 3 to diffuse reflector 6 emission light by fibre-optical probe 2; 3 pairs of these diffuse lights of spectrum analysis and data processing unit carry out spectrum analysis, and the intensity signal that draws each wavelength in this diffuse light deposits in the corresponding memory of spectrum analysis and data processing unit 3 then as the light intensity benchmark.

2. control the on off state of photoswitch 7.Connect the corresponding shunt of photoswitch 7, make the corresponding optical fiber in the launching fiber 4 in the fibre-optical probe 2 be in conducting state (being meant comprising light after launching fiber 4 penetrates the situation of the front end of the reception optical fiber 5 in the reflected back optical fiber probe 2) with the corresponding optical fiber that receives in the optical fiber 5.

3. draw the intensity signal of each wavelength in the diffuse light of biological tissue.The probe face of fibre-optical probe 2 is contacted with surface, biological tissue position to be detected, the output light of light emitted unit 1 through launching fiber 4 by fibre-optical probe 2 directive biological tissues, simultaneously, fibre-optical probe 2 receives the diffuse light of this biological tissue and transports to the reflected light input of spectrum analysis and data processing unit 3 by receiving optical fiber 5; 3 pairs of these diffuse lights of spectrum analysis and data processing unit carry out spectrum analysis, draw the intensity signal of each wavelength in the diffuse light of this biological tissue.

4. spectrum analysis and data processing unit 3 are changed the intensity signal from each wavelength in the diffuse light of tissue surface that 3. step obtains and the spectrum signal that obtains diffusing, the light intensity benchmark of the respective wavelength that 1. obtains from the intensity signal of each wavelength in the diffuse light of tissue surface and step that also to be spectrum analysis and data processing unit (3) 3. obtain step is compared, draw the sequence of a ratio, this sequence of ratio values is handled the diffuse spectrum signal or directly with this sequence of ratio values diffuse spectrum signal as biological tissue of back as biological tissue.

5. the spectrum signal that diffuses of spectrum analysis and data processing unit 3 biological tissue that 4. step is obtained is stored or is deposited in order to subsequent treatment, perhaps directly exports this spectrum signal that diffuses.

6. change photoswitch 7 on off state and to the corresponding optical fiber in the launching fiber 4 with receive corresponding optical fiber in the optical fiber 5 and be in the turn-on and turn-off state and change, thereby corresponding optical fiber in the launching fiber of conducting 4 and distance and the step distance in 2. that receives between the corresponding optical fiber in the optical fiber 5 are inequality, repeat then above-mentioned steps 3. to step 5., carry out optical detection with another position of locating different depth to biological tissue, also promptly obtain the spectrum signal that diffuses of biological tissue respective depth, then the spectrum signal that diffuses at this position is stored or deposited, in order to subsequent treatment, perhaps directly export this spectrum signal that diffuses.

7. thus repeated execution of steps 6., to finish optical detection to each degree of depth position of setting.

Above-mentioned steps 3. in, behind the light line focus coupling device 12 that the light source 11 of described light emitted unit 1 is sent, a part is passed light splitting piece 13 and is coupled in the launching fiber 4, and another part is incident upon the optical signal receiving terminal of photoelectric switching circuit 15 after the surface reflectance of light splitting piece 13; The electrical signal of photoelectric switching circuit 15 links to each other with the feedback signal input of drive circuit 16, with the intensity of stabilized light source 11 output light.

Above-mentioned steps 3. in, spectrum analysis and data processing unit 3 are when carrying out spectrum analysis, earlier by fiber spectrometer 31 or relevant device according to the diffuse light from the biological tissue that receives optical fiber 5 draw with this diffuse light in the corresponding digital signal of light intensity of each wavelength, deliver to date processing and memory element 32 or relevant device by its detection signal outfan then, thereby draw the intensity signal of each wavelength in this diffuse light by date processing and memory element 32 or relevant device according to described digital signal; Step 4. in then by data processing unit and store 32 or relevant device the intensity signal from each wavelength in the diffuse light of tissue surface that 3. step obtains is changed and the spectrum signal that obtains diffusing; The spectrum signal that diffuses of the biological tissue that 4. step is also obtained step by date processing and memory element 32 or relevant device in is 5. stored or is deposited in order to subsequent treatment, perhaps directly export this spectrum signal that diffuses, and step 5. also comprises by date processing and memory element 32 or optical detection result is delivered to display unit 33 to relevant device or relevant device shows.

(embodiment 2)

See Fig. 7, Fig. 4-2 and Fig. 5-2, all the other are identical with embodiment 1, difference is: in the present embodiment, launching fiber 4 changes single optical fiber into, receive 5 of optical fiber and be divided into two sections of front and back, the leading portion 51 that receives optical fiber 5 (can be any probable value greater than 3 for the fibre bundle with 3 optical fiber in other embodiments, as 6,10,20 etc., also can be 2, the shunt quantity of the branch terminal 72 of corresponding photoswitch 7 then is not less than the radical of optical fiber), the back segment 52 that receives optical fiber 5 is single optical fiber.The quantity of photoswitch 7 still is 1.

See Fig. 4-2 and Fig. 5-2, in the present embodiment then by the light source outfan of the preceding termination light emitted unit 1 of launching fiber 4; The rear end of each optical fiber in the leading portion 51 of reception optical fiber 5 links to each other with the optical fiber interface of a corresponding shunt of the branch terminal 72 of photoswitch 7, and 71 front ends with the back segment that receives optical fiber 5 of the single channel end of photoswitch 7 link to each other; At this moment, 71 at the single channel end of photoswitch 7 is as light output end, and the branch terminal 72 of photoswitch 7 is then as the light input; The rear end that receives the back segment of optical fiber 5 then links to each other with the reflected light input of spectrum analysis and data processing unit 3; Thereby make photoswitch 7 series connection be arranged on front end as each optical fiber in the leading portion 51 of the reception optical fiber 5 of the front end that receives optical fiber 5 to the light path between the reflected light input of spectrum analysis and data processing unit 3, even also photoswitch 7 is connected on the light source outfan of light emitted unit 1 to the light path between the reflected light input of spectrum analysis and data processing unit 3.

See Fig. 6-2,2 of the fibre-optical probes of present embodiment comprise launching fiber 4 the rear end, receive front end, shell 21 and the medical plastic fixed block 22 of each optical fiber of the leading portion 51 of optical fiber 5.Fixed block 22 still has 4 fixing holes.When making fibre-optical probe 2, penetrate in the corresponding fixing hole of fixed block 22 by the rear end of launching fiber 4 and the front end of each optical fiber that receives the leading portion 51 of optical fiber 5, stick with glue agent optical fiber and fixed block 22 are fixedly bonded together, and the end face of end face and the front end of each optical fiber of the leading portion 51 that receives optical fiber 5 of the rear end of launching fiber 4 is positioned on the same plane; In the fibre-optical probe 2, the spacing between the front end of each optical fiber of the leading portion 51 of reception optical fiber 5 and the rear end of launching fiber 4 is inequality, and the quantity of these distances inequality also equals 3; Wherein, the distance between the adjacent fiber of the leading portion 41 of reception optical fiber 5 is 5mm, and each root optical fiber on left side, centre and right side in figure of the leading portion 51 of launching fiber 4 and reception optical fiber 5 and the distance that receives between the optical fiber 5 are followed successively by 15mm, 20mm, 25mm; Distance between the end face of the end face of the front end of each optical fiber of the leading portion 51 of reception optical fiber 5 and the rear end of launching fiber 4 can be set in 10～30mm when making fibre-optical probe 2, be suitable for receiving survey diffuse light on the different aspects in the biological tissue, carry out optical detection with biological tissue to subcutaneous 4～6cm place.

(embodiment 3)

See Fig. 8, Fig. 4-3 and Fig. 5-3, all the other are identical with embodiment 1, and difference is: receive 5 of optical fiber and be divided into two sections of front and back, the leading portion 51 that receives optical fiber 5 is for having the fibre bundle of 2 optical fiber, and the back segment 52 that receives optical fiber 5 is single optical fiber.The quantity of photoswitch 7 is 2, and first photoswitch 7 be arranged on by the series connection of its optical port launching fiber 4 leading portion 41 optical fiber rear end and each optical fiber of the back segment 42 of launching fiber 4 front end with between, second photoswitch 7 is arranged on by its optical port series connection between the front end of optical fiber of back segment 52 of the rear end of each optical fiber of leading portion 51 of reception optical fiber 5 and reception optical fiber 5.

See Fig. 6-3,2 of the fibre-optical probes of present embodiment comprise launching fiber 4 back segment 42 each optical fiber the rear end, receive front end, shell 21 and the medical plastic fixed block 22 of each optical fiber of the leading portion 51 of optical fiber 5.Fixed block 22 has 5 fixing holes.When making fibre-optical probe 2, penetrate in the corresponding fixing hole of fixed block 22 by the rear end of the back segment 42 of launching fiber 4 and the front end that receives the leading portion 51 of optical fiber 5, stick with glue agent optical fiber and fixed block 22 are fixedly bonded together, and the end face of end face and each optical fiber front end of the leading portion 51 that receives optical fiber 5 of each optical fiber rear end of the back segment 42 of launching fiber 4 is positioned on the same plane; Spacing between the end face of each optical fiber rear end of the end face of each optical fiber front end of the leading portion 51 of reception optical fiber 5 and the back segment 42 of launching fiber 4 is inequality, and the quantity of these distances inequality equals 6; Wherein, the back segment 42 of launching fiber 4 in figure each root optical fiber and the reception optical fiber 5 on left side, centre and right side leading portion 51 in figure above an optical fiber between distance be about 25mm, 20mm, 15mm successively, and and receive optical fiber 5 leading portion 51 in figure below an optical fiber between distance be about 27.5mm, 22.5mm, 17.5mm successively, and the distance between the adjacent fiber of the back segment 41 of launching fiber 4 is about 5 millimeters, and the distance between two optical fiber of the leading portion 51 of reception optical fiber 5 also is about 5 millimeters.

(embodiment 4)

See Fig. 8, Fig. 4-3, Fig. 5-4 and Fig. 6-4, all the other are identical with embodiment 3, and difference is: the number of fibers of the back segment 42 of launching fiber 4 is 2, and the number of fibers that receives the leading portion 51 of optical fiber 5 is 3.The shape of the plastics fixed block 22 in the fibre-optical probe 2 is shown in Fig. 6-4.

See Fig. 6-4, being arranged on the left of the figure of the optical fiber that is arranged in the figure below of the back segment 42 of launching fiber 4 and the leading portion 51 of reception optical fiber 5, middle, distance between each root optical fiber of right side is about 15mm successively, 20mm, 25mm, and the optical fiber that is arranged in figure top of the back segment 42 of launching fiber 4 and the leading portion 51 that receives optical fiber 5 be arranged in the figure left side, middle, distance between each root optical fiber of right side is about 17.5mm successively, 22.5mm, 27.5mm, and the distance between two optical fiber of the back segment 41 of launching fiber 4 is about 5 millimeters, and the distance between the adjacent fiber of the leading portion 51 of reception optical fiber 5 also is about 5 millimeters.

(embodiment 5)

See Fig. 9, all the other are identical with embodiment 1, and difference is: light emitted unit 1 sends the device of dual wavelength light when being to use, and one of them wavelength is 660nm, and another wavelength is 940nm.Serve as light source by 2 light emitting diodes, in addition, light emitted unit 1 also has light source driving circuit and double pulse generator except that light source, but does not have focusing coupling device 12, light splitting piece 13 and photoelectric switching circuit 15 etc. among the embodiment 1.

In spectrum analysis in the present embodiment and the data processing unit 3, by the fiber spectrometer 31 in light sensitive diode, signal amplification circuit, analog to digital conversion circuit and the single-chip microcomputer alternate embodiment 1, computer then plays the effect of date processing and memory element 32, and then plays the effect of display unit 33 with the supporting display of computer.

In the present embodiment, the control of photoswitch 7 can adopt the electric pulse of time series to carry out automatic light path conversion.Photoswitch 7 in the foregoing description 1 to embodiment 4 also can adopt this mode to control.

(embodiment 6)

See Fig. 6-5, all the other are identical with embodiment 1, and difference is: the reception optical fiber 5 of present embodiment is the fibre bundle of three optical fiber, and three optical fiber combine.Though the fixed block 22 of fibre-optical probe 2 also is to have 4 fixing holes, but it is bigger to be used for fixing the fixing hole that receives optical fiber 5, can hold three optical fiber, the gap that is provided with in the fixing hole that receives optical fiber 5 front ends of fixed block 22 can be filled with the filler that has medical binding agent.

The fiber spectrometer 31 of the spectrum analysis of present embodiment and data processing unit 3 is the equipment with a plurality of optical fiber interfaces, and the rear end that receives each optical fiber of optical fiber 5 is connected with the corresponding optical fiber interface of fiber spectrometer 31 simultaneously.The checkout gear of present embodiment combines because receive three optical fiber of optical fiber 5 in use like this, so when this device receives diffuse light, can play the effect that increases luminous flux, helps improving the sensitivity of detection.

In the present embodiment, for the part of the fixed block that is arranged in fibre-optical probe 2 of three optical fiber that receive optical fiber 5, because they abut against together, so think that in design process the spacing of these compact arranged optical fiber is zero.

(embodiment 7)

See Fig. 6-6, all the other are identical with embodiment 2, and difference is: the launching fiber 4 of present embodiment is the fibre bundle of three optical fiber, and three optical fiber combine.Though the fixed block 22 of fibre-optical probe 2 also is to have 4 fixing holes, but the fixing hole that is used for fixing launching fiber 4 is bigger, can hold three optical fiber, the gap in the fixing hole that is provided with launching fiber 4 front ends of fixed block 22 can be filled with the filler that has medical binding agent.

The light emitted unit 1 of present embodiment is the equipment with a plurality of optical fiber interfaces, and the front end of each optical fiber of launching fiber 4 is connected with the corresponding optical fiber interface of light emitted unit 1 simultaneously.Like this checkout gear of present embodiment in use because three optical fiber of launching fiber 4 combine, thus when this device emission light, can play the effect that increases luminous flux, thereby help improving the sensitivity of detection.

In the present embodiment, for the part of the fixed block that is arranged in fibre-optical probe 2 of three optical fiber of launching fiber 4, because they abut against together, so think that in design process the spacing of these compact arranged optical fiber is zero.

(embodiment 8, biological tissue optical detection apparatus)

All the other are identical with embodiment 1, difference is: this checkout gear also comprises a memorizer that is used to store the spectra database of the same type biological tissue diffuse reflection spectrum of gathering in advance, and this memorizer is provided with separately or is arranged in the date processing and memory element 32 of spectrum analysis and data processing unit 3.During the date processing of spectrum analysis and data processing unit 3 and memory element 32 also are to use behind the spectrum signal that obtains diffusing again to described spectra database in each diffuse reflection spectrum of same type biological tissue carry out the similarity contrast and find out similar diffuse reflection spectrum among the data base, belong to the biological tissue of which kind of state and then draw the device of comparison result by this similar diffuse reflection spectrum, at last by outside these comparison results of output of the display unit 33 of spectrum analysis and data processing unit 3.

The method of work ground remainder of the biological tissue optical detection apparatus of present embodiment is identical with the method for work of the device of embodiment 1, difference is: step 5. in, the date processing of spectrum analysis and data processing unit 3 and memory element 32 or relevant device are deposited the spectrum signal that diffuses, again the spectrum signal of depositing that diffuses is carried out following post processing: each diffuse reflection spectrum of the same type biological tissue in will diffuse spectrum signal and the described spectra database carries out the similarity contrast and finds out similar diffuse reflection spectrum among the data base, belonged to the biological tissue of which kind of state and then drawn comparison result by this similar diffuse reflection spectrum, output is in the normal condition or the comparing result of abnormal condition then.

Above-mentioned similarity contrast is meant when the spectrum signal that diffuses that has at least the 3 sections characteristic spectral line variation tendencies and the checkout gear of present embodiment to detect for the same type biological tissue diffuse reflection spectrum of gathering in advance is identical, just is defined as having similarity.

(application examples 1)

See Figure 11, on the basis of the method for work of the device of embodiment 8, be further processed by the date processing of spectrum analysis and data processing unit 3 and memory element 32 or relevant device for the spectrum information that diffuses of the biological tissue that is in abnormal condition.If the type of biological tissue is a breast, then the various diffuse reflection spectrums of the breast tissue in the diffuse spectrum signal and the spectra database of breast compare, whether the characteristic spectral line of judging the spectrum information that diffuses of biological tissue successively conforms to the characteristic spectral line of characteristic spectral line, the characteristic spectral line of breast fibroadenoma, the carcinoid characteristic spectral line of breast and the breast carcinoma of cyclomastopathy, situation about being consistent for spectral line, then judge and specifically belong to any abnormal condition, make diagnosis then, show this diagnostic result by display unit 33 or relevant device at last; When if the feature of the various diffuse reflection spectrums of breast tissue does not all conform in diffuse spectrum signal and the described spectra database of described breast, then display unit 33 or relevant device display reminding information: suggestion detects or adopts other means detection again.

If checkout gear also comprises a memorizer that stores the tumor cases data base of the various biological tissues of gathering in advance, and this memorizer is when being provided with or being arranged in the date processing of spectrum analysis and data processing unit 3 and memory element 32 or the relevant device separately, after making above-mentioned diagnosis, read corresponding pathology picture and doctor's advice among the tumor cases data base according to comparing result, and show by the display unit 33 or the relevant device of spectrum analysis and data processing unit 3.

(application examples 2)

See Figure 12 and Figure 13, should on the basis of the method for work of the device of embodiment 8, carry out by use-case.Figure 12 is the diffuse reflection spectrum that is stored in the normal gastric mucosa surface in the memorizer of spectra database, Figure 13 is stored in the gastric cancer in the memorizer of spectra database or the diffuse reflection spectrum of adenocarcinoma mucomembranous surface, and obviously gastric cancer or adenocarcinoma mucomembranous surface are that light between the 600nm to 700nm absorbs more to wavelength.So,, then can draw the stomach of being surveyed and have gastric cancer if the Figure 13 in diffuse spectrum information and the spectra database that date processing and memory element 32 or relevant device draw is close to when the intravital gastric mucosa of people detects.

(application examples 3)

See Figure 14 and Figure 15, should on the basis of the method for work of the device of embodiment 8, carry out by use-case.Figure 14 is the diffuse reflection spectrum that is stored in the normal esophagus in the memorizer of spectra database, and Figure 15 is the diffuse reflection spectrum that is stored in the canceration esophagus in the memorizer of spectra database; Obviously it is balanced that normal esophagus is to wavelength that light between the 400nm to 800nm absorbs, and that the canceration esophagus is a light absorption difference between the 400nm to 800nm is bigger.So,, then can draw the esophageal-gastric of being surveyed and have canceration if the Figure 15 in diffuse spectrum information and the spectra database that date processing and memory element 32 or relevant device draw is close to when the intravital esophagus of people detects.

Date processing and memory element 32 or relevant device are scalable or dwindle the spectrum picture that display unit 33 or relevant device show in above-mentioned three application examples.

Date processing and memory element 32 or relevant device also can connect a printer, to print case report, so that the patient uses.Also can link to each other, be used to send the Email that contains case report with the Internet.

Obviously, the foregoing description of the present utility model only is for this utility model example clearly is described, and is not to be qualification to embodiment of the present utility model.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here need not also can't give exhaustive to all embodiments.And these belong to conspicuous variation or the change that spirit of the present utility model extended out and still are among the protection domain of the present utility model.

Claims

1. a biological tissue optical detection apparatus has the main apparatus main body of detection, detects main apparatus main body and comprises: light emitted unit (1), fibre-optical probe (2), spectrum analysis and data processing unit (3), launching fiber (4) and reception optical fiber (5); The light source outfan of the preceding termination light emitted unit (1) of launching fiber (4) receives the back termination spectrum analysis of optical fiber (5) and the reflected light input of data processing unit (3); Fibre-optical probe (2) comprises the rear end of launching fiber (4) and receives the front end of optical fiber (5); The light that the light source outfan of light emitted unit (1) is sent when being to use is through the outfan of launching fiber (4) by fibre-optical probe (2) directive outside, and the light that the reflected light input of spectrum analysis and data processing unit (3) is received when being to use is from port outside and that this light transmits through reception optical fiber (5); Spectrum analysis and data processing unit (3) can be handled the reflection ray that is received when being to use and draw the intensity signal of wherein each wavelength and then obtain the device of reflected light spectral signal; It is characterized in that:

Detect main apparatus main body and also comprise photoswitch (7); Fibre-optical probe (2) is the harmless probe of surface contact; Photoswitch (7) has light input and two optical ports of light output end, and in these two optical ports: a port is single channel end (71), and another port is the branch terminal (72) that has 2 shunt at least; The then corresponding still chromatography type fibre-optical probe of fibre-optical probe (2), they also be that every optical fiber in the launching fiber (4) in the fibre-optical probe (2) and the distance that receives between the every optical fiber in the optical fiber (5) are all or part of inequality, and the quantity of these distances inequality is more than or equal to 2; Photoswitch (7) is connected on light emitted unit (1) by its optical port light source outfan to the light path between the rear end of launching fiber (4) or/and be connected on receive optical fiber (5) front end to the light path between the reflected light input of spectrum analysis and data processing unit (3), also be that photoswitch (7) is connected on the light source outfan of light emitted unit (1) to the light path between the reflected light input of spectrum analysis and data processing unit (3); Photoswitch (7) when being to use because of anastomosing shunt difference corresponding optical fiber in the launching fiber (4) in the fibre-optical probe (2) and the corresponding optical fiber that receives in the optical fiber (5) are in the photoswitch that the turn-on and turn-off state is controlled.

2. biological tissue optical detection apparatus according to claim 1, it is characterized in that: launching fiber (4) is divided into leading portion and back segment or/and receive optical fiber (5), and the back segment of launching fiber (4) is or/and the leading portion of reception optical fiber (5) is the fibre bundle with at least 2 optical fiber; Wherein,

When photoswitch (7) is connected on the light path of light source outfan between the rear end of launching fiber (4) of described light emitted unit (1) by its optical port, receiving optical fiber (5) is single optical fiber, launching fiber (4) is divided into leading portion (41) and back segment (42), the leading portion (41) of launching fiber (4) has at least 1 optical fiber, and the back segment (42) of launching fiber (4) is for having the fibre bundle of at least 2 optical fiber; The light source outfan of light emitted unit (1) then links to each other with front end as the leading portion (41) of the launching fiber (4) of launching fiber (4) front end, the single channel end (71) of the back termination photoswitch (7) of the leading portion (41) of launching fiber (4), the single channel end (71) of this moment is then as the light input; Each of the branch terminal (72) of photoswitch (7) links to each other with the front end of a corresponding optical fiber in the back segment (42) of launching fiber (4) along separate routes, and branch terminal (72) at this moment is then as light output end; Have nothing in common with each other as the rear end of each optical fiber in the back segment (42) of the launching fiber (4) of the rear end of launching fiber (4) spacing with the front end that receives optical fiber (5);

When photoswitch (7) is connected on the light path of front end between the reflected light input of spectrum analysis and data processing unit (3) of described reception optical fiber (5) by its optical port, launching fiber (4) is single optical fiber, receive optical fiber (5) and be divided into leading portion (51) and back segment (52), the leading portion (51) that receives optical fiber (5) is for having the fibre bundle of at least 2 optical fiber, and the back segment (52) that receives optical fiber (5) has at least 1 optical fiber; Spacing as the rear end of the front end of each optical fiber in the leading portion (51) of the reception optical fiber (5) of the front end that receives optical fiber (5) and launching fiber (4) has nothing in common with each other; The rear end of each optical fiber in the leading portion (51) of reception optical fiber (5) links to each other with a corresponding shunt of the branch terminal (72) of photoswitch (7), and the branch terminal (72) of this moment is then as the light input; The single channel end (71) of photoswitch (7) links to each other with the front end of the back segment (52) that receives optical fiber (5), and the single channel end (71) of this moment is then as light output end; Rear end as the back segment (52) of the reception optical fiber (5) of the rear end that receives optical fiber (5) then links to each other with the reflected light input of spectrum analysis and data processing unit (3);

When photoswitch (7) is connected on light emitted unit (1) by its optical port light source outfan to the light path between the rear end of launching fiber (4) and when being connected on the light path of front end between the reflected light input of spectrum analysis and data processing unit (3) that receives optical fiber (5), the quantity of photoswitch (7) is 2, and first photoswitch (7) is connected on the light source outfan of described light emitted unit (1) to the light path between the rear end of launching fiber (4) by its optical port, and second photoswitch (7) is connected on the front end of described reception optical fiber (5) to the light path between the reflected light input of spectrum analysis and data processing unit (3); Launching fiber (4) is divided into leading portion (41) and back segment (42), and the leading portion (41) of launching fiber (4) has at least 1 optical fiber, and the back segment (42) of launching fiber (4) is for having the fibre bundle of at least 2 optical fiber; The light source outfan of light emitted unit (1) then links to each other with front end as the leading portion (41) of the launching fiber (4) of launching fiber (4) front end, the single channel end (71) of first photoswitch of back termination (7) of the leading portion (41) of launching fiber (4), the single channel end (71) of this moment is then as the light input; Each of the branch terminal (72) of first photoswitch (7) links to each other with the front end of a corresponding optical fiber in the back segment (42) of launching fiber (4) along separate routes, and branch terminal (72) at this moment is then as light output end; Receive optical fiber (5) and also be divided into leading portion (51) and back segment (52), the leading portion (51) that receives optical fiber (5) is for having the fibre bundle of at least 2 optical fiber, and the back segment (52) that receives optical fiber (5) has at least 1 optical fiber; Have nothing in common with each other as the rear end of each optical fiber in the back segment (42) of the launching fiber (4) of the rear end of launching fiber (4) and spacing as the front end of each optical fiber in the leading portion (51) of the reception optical fiber (5) of the front end that receives optical fiber (5); The rear end of each optical fiber in the leading portion (51) of reception optical fiber (5) links to each other with a corresponding shunt of the branch terminal (72) of second photoswitch (7), and the branch terminal (72) of this moment is then as the light input; The single channel end (71) of second photoswitch (7) links to each other with the front end of the back segment (52) that receives optical fiber (5), and the single channel end (71) of this moment is then as light output end; Rear end as the back segment (52) of the reception optical fiber (5) of the rear end that receives optical fiber (5) then links to each other with the reflected light input of spectrum analysis and data processing unit (3).

3. biological tissue optical detection apparatus according to claim 1, it is characterized in that: described light source incident unit (1) sends the device that wave-length coverage is 400～1000 nanometer light when being to use, the light that sends when being to use specifically is the light of 2～4 wavelength of wavelength in 400～1000 nanometer wavelength range, or the waveband width of wavelength in 400～1000 nanometer wavelength range be the light of the continuous wavelength of 100～600 nanometers, or have successive white-light spectrum, its wave band is the light of visible light wave range and/or near infrared band.

4. biological tissue optical detection apparatus according to claim 1 is characterized in that: described light emitted unit (1) has light source (11), focuses on coupling device (12), light splitting piece (13), photoelectric switching circuit (15), drive circuit (16) and power supply (17); Power supply (17) links to each other through the power end of control circuit (16) with light source (11); On the light path of light emitted unit (1), be provided with light source (11) successively, focus on coupling device (12), light splitting piece (13) and photoelectric switching circuit (15); Behind the light line focus coupling device (12) that sends from light source (11), part light passes light splitting piece (13) and is coupled in the launching fiber (4), and another part light is incident upon the optical signal receiving terminal of photoelectric switching circuit (15) after the surface reflectance of light splitting piece (13); The electrical signal of photoelectric switching circuit (15) links to each other with the feedback signal input of drive circuit (16), with the intensity of stabilized light source (11) output light.

5. biological tissue optical detection apparatus according to claim 1 is characterized in that: spectrum analysis and data processing unit (3) also comprise a memorizer that stores each wavelength light strong basis standard of diffuse light; Then under the situation of reflection ray that spectrum analysis and data processing unit (3) are received when using as diffuse light, spectrum analysis and data processing unit (3) can obtain the intensity signal of each wavelength in the diffuse light when also being to use, intensity signal to this each wavelength is changed and the spectrum signal that obtains diffusing, the device of last this spectrum signal that diffuses of outwards output; Above-mentioned intensity signal conversion to each wavelength is meant: the light intensity benchmark with the respective wavelength in the intensity signal of this each wavelength and each wavelength light strong basis standard of being stored is compared the sequence that obtains a ratio, and this sequence of ratio values is handled the back as the spectrum signal or direct as the spectrum signal that diffuses that diffuses.

6. biological tissue optical detection apparatus according to claim 5 is characterized in that: spectrum analysis and data processing unit (3) comprise fiber spectrometer (31), date processing and memory element (32) and the display unit (33) that is electrically connected successively; Fiber spectrometer (31) has optical signal input and detection signal outfan, and the optical signal input of fiber spectrometer (31) is the reflected light input of spectrum analysis and data processing unit (3); The memorizer that stores each wavelength light strong basis standard of diffuse light is arranged in date processing and the memory element (32); Fiber spectrometer (31) can receive the device from the light that receives optical fiber (5) when being to use, fiber spectrometer (31) can draw when also being to use with this reception light in the corresponding digital signal of light intensity of each wavelength, and then export the device of this digital signal by its detection signal outfan, and if this reception light is incident upon and can carries out irreflexive object to light to the outside for the light that this device sends, carry out diffuse-reflectance by this object again and when receiving optical fiber (5) and transport to the light of fiber spectrometer (31), then fiber spectrometer (31) can draw when being to use with this diffuse light in the corresponding digital signal of light intensity of each wavelength, and then export the device of this digital signal by its detection signal outfan; Date processing and memory element (32) can be received the device of above-mentioned digital signal by its detection signal input when being to use; Date processing and memory element (32) can draw the device of the intensity signal of each wavelength in the corresponding light according to described digital signal when also being to use, and date processing and memory element (32) are still changed and the device of the spectrum signal that obtains diffusing the intensity signal of each wavelength; Display unit (33) can receive the above-mentioned spectrum signal that diffuses, again this signal be carried out device shown when being to use.

7. biological tissue optical detection apparatus according to claim 5, it is characterized in that: also comprise a memorizer that is used to store the spectra database of the same type biological tissue diffuse reflection spectrum of gathering in advance, and this memorizer is provided with separately or is arranged in spectrum analysis and the data processing unit (3); During spectrum analysis and data processing unit (3) also are to use behind the spectrum signal that obtains diffusing again to described spectra database in each diffuse reflection spectrum of same type biological tissue carry out the similarity contrast and find out similar diffuse reflection spectrum among the data base, belong to the biological tissue of which kind of state and then draw comparison result by this similar diffuse reflection spectrum, the device of last this comparison result of outwards output.

8. according to the described biological tissue optical detection apparatus of one of claim 1 to 7, it is characterized in that: also comprise being independent of the diffuse reflector (6) that detects main device; The reflectance of this diffuse reflector (6) is 12% to 30%, during being to use, this diffuse reflector (6) will detect the parts of the light diffuse-reflectance back into optical fibers probe (2) that the fibre-optical probe (2) of main device sends, so that spectrum analysis and data processing unit (3) carry out spectrum analysis to this diffuse light, draw each wavelength in the diffuse light of this moment intensity signal and as the light intensity benchmark of reflection ray.

9. according to the described biological tissue optical detection apparatus of one of claim 1 to 7, it is characterized in that: fibre-optical probe (2) also has fixed block (22), fixed block (22) is provided with fixing hole, and the front end setting of each optical fiber of the rear end of each optical fiber of launching fiber (4) and reception optical fiber (5) also is fixed in the corresponding fixing hole of fixed block (22).