CN111992339B

CN111992339B - Centrifugal separation device, and method for separating and culturing mixed liquid

Info

Publication number: CN111992339B
Application number: CN202010741366.XA
Authority: CN
Inventors: 刘肖琳; 丁晓华; 赵明; 于起峰; 陈鸣; 周玥
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-07-28
Filing date: 2020-07-28
Publication date: 2022-02-11
Anticipated expiration: 2040-07-28
Also published as: CN111992339A

Abstract

The application provides a centrifugal separation device, includes: a centrifuge tank for centrifuging a mixed solution, the mixed solution including at least two monomer liquids; one end of the liquid guide pipeline is communicated with the inner cavity of the centrifugal tank and is used for guiding the mixed liquid into the inner cavity of the centrifugal tank or guiding the mixed liquid out of the inner cavity of the centrifugal tank; the storage tank is connected with the centrifugal tank and is provided with a connecting cavity and a storage cavity, the connecting cavity is communicated between the storage cavity and an inner cavity of the centrifugal tank, and the storage cavity is used for storing monomer liquid separated from the mixed liquid; the switch assembly is connected with the storage tank and used for controlling the connection cavity to be conducted or plugged; and one end of the pipette is communicated with the storage chamber and is used for leading out the monomer liquid in the storage chamber. The application also provides a mixed liquid separation and culture method. The application provides a centrifugal separation device can improve mixed liquid separation efficiency, purity and stability.

Description

Centrifugal separation device, and method for separating and culturing mixed liquid

Technical Field

The application relates to the technical field of centrifugal separation, in particular to a centrifugal separation device and a mixed liquid separation and culture method.

Background

Cell specific gravity separation technology is one of the most widely used cell separation technologies. At present, the current application situation of the similar technology is almost that the technical operations of cell separation, cell proliferation culture, cell washing and the like are completed separately, so that the efficiency of in vitro cell preparation is greatly reduced, and the possibility of external pollution of a separated sample is greatly increased. In the existing extremely limited integrated treatment technology of cell separation and cell proliferation culture, the defects of complex structure of consumable materials used for cell separation culture, high standard of manufacturing materials, large difficulty of manufacturing process, high production cost of products and the like exist, and the cell separation efficiency, purity and stability of the cell separation culture also need to be further improved.

Disclosure of Invention

The application provides a centrifugal separation device capable of improving separation efficiency, purity and stability, and a mixed liquid separation and culture method.

In a first aspect, an embodiment of the present application provides a centrifugal separation device, including: a centrifuge tank for centrifuging a mixed liquor, the mixed liquor comprising at least two monomer fluids;

one end of the liquid guide pipeline is communicated with the inner cavity of the centrifugal tank and is used for guiding the mixed liquid into the inner cavity of the centrifugal tank or guiding the mixed liquid out of the inner cavity of the centrifugal tank;

the storage tank is connected with the centrifugal tank and is provided with a connecting cavity and a storage cavity, the connecting cavity is communicated between the storage cavity and an inner cavity of the centrifugal tank, and the storage cavity is used for storing monomer liquid separated from the mixed liquid;

the switch assembly is connected with the storage tank and used for controlling the connection cavity to be conducted or blocked; and

and one end of the pipette is communicated with the storage cavity and is used for leading out the monomer liquid in the storage cavity.

In a second aspect, the mixed liquid separating and culturing method provided in the embodiment of the present application is applied to a centrifugal separation device, where the centrifugal separation device includes at least one liquid guiding pipe, a centrifugal tank, at least one storage tank, at least one switch component, and at least one pipette, and an inner cavity of the centrifugal tank is in communication with one end of the liquid guiding pipe, and is used for centrifuging the mixed liquid; the storage tank is connected with the centrifugal tank, the storage tank is provided with a connecting cavity and a storage cavity, and the connecting cavity is communicated between the storage cavity and an inner cavity of the centrifugal tank; the switch assembly is connected with the storage tank; one end of the pipette is communicated with the storage cavity; the method comprises the following steps:

injecting the mixed solution into the centrifugal tank through the liquid guide pipeline;

driving the centrifugal tank to rotate;

detecting whether the mixed liquid forms various layered monomer liquids along the inner side wall of the centrifugal tank or not, and if so, determining a layer where a target monomer liquid is located in the multiple layers of monomer liquids;

controlling the switch assembly to switch on or off the storage cavity and the inner cavity of the centrifugal tank so as to lead out the monomer liquid on the outer layer of the target monomer liquid from the storage tank and the pipette, store the target monomer liquid into the storage tank, and lead out the monomer liquid on the inner layer of the target monomer liquid from the centrifugal tank through the liquid guide pipeline;

delivering the target monomer fluid to the centrifuge tank;

and injecting a culture solution into the centrifugal tank through the liquid guide pipeline so as to culture the target monomer liquid.

According to the centrifugal separation device provided by the embodiment of the application, the inner cavity of the centrifugal tank is communicated with one end of the liquid guide pipeline, the connecting cavity of the storage tank is communicated between the storage cavity and the inner cavity of the centrifugal tank, the switch assembly is connected with the storage tank to control the connection and disconnection of the connecting cavity, one end of the pipette is communicated with the storage cavity, and mixed liquid is injected into the centrifugal tank through the liquid guide pipeline; driving the centrifugal tank to rotate to centrifuge the mixed solution; detecting whether the mixed solution forms various layered monomer solutions along the inner side wall of the centrifugal tank, and if so, determining the layer of the target monomer solution in the multilayer monomer solution; the control switch assembly is used for switching on or off the storage cavity and the inner cavity of the centrifugal tank so as to lead out the monomer liquid on the outer layer of the target monomer liquid through the storage tank and the pipette, store the target monomer liquid into the storage tank and lead out the monomer liquid on the inner layer of the target monomer liquid from the centrifugal tank through the liquid guide pipeline; conveying the target monomer liquid to a centrifugal tank; injecting the culture solution into a centrifugal tank through a solution guide pipeline to culture the target monomer solution; the centrifugal separation device can realize the integration of operations such as the separation of monomer liquid in mixed liquid, the culture of target monomer liquid, the washing of the centrifugal separation device and the like; when the mixed liquid is blood, the separation purity and the separation efficiency of various cell suspension samples with small specific gravity difference can be improved, the position stability of various cells with different types (specific gravity) in the separation process is continuously kept, and the phenomenon of secondary mixing in the separation process is avoided, so that the separation purity is influenced; and the micro target cells can be accurately separated and extracted, so that the possibility that the sample is separated due to the pollution of the external environment in the separation process is avoided.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.

FIG. 1 is a schematic structural diagram of a centrifugal separation apparatus provided in an embodiment of the present application;

FIG. 2 is a schematic partially broken away illustration of a centrifugal separation apparatus provided in FIG. 1;

FIG. 3 is a cross-sectional view of a centrifugal separation device provided in FIG. 1;

FIG. 4 is a partially enlarged view showing the first centrifugal separator device shown in FIG. 3;

FIG. 5 is a schematic structural diagram of a centrifuge tank provided in an embodiment of the present application;

FIG. 6 is a schematic structural diagram of a centrifugal separation apparatus provided in the second embodiment of the present application;

FIG. 7 is a schematic partially broken away view of a centrifugal separation device provided in FIG. 6;

FIG. 8 is a cross-sectional view of a centrifugal separation device provided in FIG. 7;

FIG. 9 is a schematic diagram of another centrifuge tank according to an embodiment of the present disclosure;

FIG. 10 is a schematic diagram of a centrifuge tank according to an embodiment of the present disclosure;

FIG. 11 is a second enlarged partial schematic view of a centrifugal separation device provided in FIG. 3;

FIG. 12 is a flowchart of a mixed solution separation and culture method according to an embodiment of the present application.

Detailed Description

The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. The embodiments listed in the present application may be appropriately combined with each other.

The centrifugal separation device 100 provided by the embodiment of the present application is applied to separating mixed liquid. The mixed solution comprises a plurality of monomer liquids with different specific gravities. The mixed solution includes, but is not limited to, blood, cell mixed solution, various bacteria culture solution, etc. The present application exemplifies the separation of blood by the centrifugal separation apparatus 100.

Referring to fig. 1 and 2, the centrifugal separation apparatus 100 includes at least one liquid guiding pipe 1, a centrifugal tank 3, at least one storage tank 5, at least one switch assembly 7, and at least one pipette 9.

Referring to fig. 2, the liquid guiding pipe 1 is a thin pipe. One port of the liquid guiding pipe 1 is an inlet for injecting the mixed liquid into the centrifugal separation device 100. The other end of the liquid guide pipeline 1 is communicated with the inner cavity 31 of the centrifugal tank 3. The liquid guiding pipe 1 is used for guiding and transferring the mixed liquid to the inner cavity 31 of the centrifugal tank 3.

The inner cavity 31 of the centrifuge tank 3 is a closed cavity. The inner chamber 31 of the centrifuge tank 3 is used for storing the mixed liquid. In this embodiment, the mixed solution is blood. It is understood that the centrifugal separation device 100 further comprises a driving motor connected to the centrifugal tank 3 for driving the centrifugal tank 3 to rotate to centrifuge the mixed liquid so that the mixed liquid is layered along the inner wall of the centrifugal tank 3 under the centrifugal force.

Referring to fig. 3 and 4, the storage tank 5 is located outside the centrifuge tank 3 and connected to the centrifuge tank 3. The storage tank 5 has a connection chamber 51 and a storage chamber 52. The connecting chamber 51 opens between the storage chamber 52 and the inner chamber 31 of the centrifuge tank 3. The storage chamber 52 is used to store the monomer liquid separated from the mixed liquid. A storage chamber 52 stores a single body fluid. In this embodiment, the mixed solution is blood, and the monomer solution may be white blood cells, red blood cells, plasma, or the like in blood. It will be appreciated that the volume of the storage chamber 52 is less than the volume of the internal chamber 31 of the centrifuge tank 3.

Referring to fig. 3 and 4, the switch assembly 7 is connected to the storage tank 5. The switch assembly 7 is used for controlling the connection cavity 51 to be conducted or sealed. The switch assembly 7 includes, but is not limited to, a pneumatic switch valve, an electric switch valve, a manual switch valve, and the like. The structure of the pneumatic switch valve is approximately the same as that of the electric switch valve, and the main difference is that the pushing force for pushing the sliding block in the valve body to change the valve position is different. The pneumatic switch valve is characterized in that control air in an air passage (pipe) is sent to an operating cylinder in the valve to push a piston to move so as to drive a sliding block to change the valve position of a valve body; the electric switch valve generates electromagnetic attraction force through the spiral coil to drive the sliding block to change the valve position of the valve body. The manual valve opening and closing is realized by changing the valve position of the valve body through manually rotating, pushing or pulling the sliding block. The embodiment of the present application is exemplified by an electric on-off valve, and will be described in detail later.

Referring to fig. 4, the pipette 9 is a thin tube, and one end of the pipette 9 is connected to the storage chamber 52 for guiding out the monomer liquid in the storage chamber 52.

Optionally, the number of the liquid guiding pipes 1 is one, the number of the storage tanks 5 is one, the number of the switch assemblies 7 is one, and the number of the pipettes 9 is one. In this embodiment, referring to fig. 1 and fig. 3, the number of the liquid guiding pipes 1 is one, the number of the storage tanks 5 is plural, the number of the switch assemblies 7 is plural, and the number of the switch assemblies 7 is equal to the number of the storage tanks 5. The number of pipettes 9 is plural, and the number of pipettes 9 may be equal to the number of storage tanks 5, or the number of pipettes 9 may be smaller than the number of storage tanks 5. Of course, in other embodiments, the number of the liquid guide pipes 1 may be multiple.

The centrifugal separation device 100 provided by the embodiment of the application is provided with the inner cavity 31 of the centrifugal tank 3 and one end of the liquid guide pipeline 1 which are communicated, the connecting cavity 51 of the storage tank 5 is communicated between the storage cavity 52 and the inner cavity 31 of the centrifugal tank 3, the switch component 7 is connected with the storage tank 5 to control the connection and disconnection of the connecting cavity 51, one end of the pipette 9 is communicated with the storage cavity 52, and mixed liquid is injected into the centrifugal tank 3 through the liquid guide pipeline 1; driving the centrifugal tank 3 to rotate to centrifuge the mixed liquid; detecting whether the mixed solution forms various layered monomer solutions along the inner side wall of the centrifugal tank 3, and if so, determining the layer of the target monomer solution in the multilayer monomer solution; the control switch assembly 7 is used for switching on or off the storage cavity 52 and the inner cavity 31 of the centrifugal tank 3 so as to lead out the monomer liquid on the outer layer of the target monomer liquid from the storage tank 5 and the pipette 9, store the target monomer liquid into the storage tank 5 and lead out the monomer liquid on the inner layer of the target monomer liquid from the centrifugal tank 3 through the liquid guide pipeline 1; conveying the target monomer liquid to a centrifugal tank 3; injecting the culture solution into a centrifugal tank 3 through a liquid guide pipeline 1 to culture the target monomer liquid; the centrifugal separator 100 described above can integrate operations such as separation of a monomer liquid in a mixed solution, culture of a target monomer liquid, and washing of the centrifugal separator 100; when the mixed liquid is blood, the separation purity and the separation efficiency of various cell suspension samples with small specific gravity difference can be improved, the position stability of various cells with different types (specific gravity) in the separation process is continuously kept, and the phenomenon of secondary mixing in the separation process is avoided, so that the separation purity is influenced; and the micro target cells can be accurately separated and extracted, so that the possibility that the sample is separated due to the pollution of the external environment in the separation process is avoided.

Referring to fig. 1 and 2, the centrifuge tank 3 includes a tank 32 and a top cover 33 covering the tank 32. The material and capacity of the can 32 are not specifically limited in this application. The tank 32 has a large capacity so that the mixed liquid can be centrifuged in the tank 32 to form an annular layered structure on the inner wall of the tank 32.

Referring to fig. 2, in the present embodiment, the radial dimension of at least a portion of the inner cavity of the can 32 gradually changes along the axial direction of the can 32.

Specifically, the axial direction of the can 32 is the height direction of the can 32. The radial direction is a direction lying in a horizontal plane perpendicular to the axial direction. The horizontal cross section of the side wall of the inner cavity of the tank 32 can be circular, polygonal, irregular, etc.

Referring to fig. 3 and 4, the sidewall of the inner cavity of the can 32 includes a liquid-fetching surface 321 in a ring shape. The liquid-taking surface 321 is provided with at least one communicating hole 322. The through hole 322 is in communication with the connection chamber 51 of the storage tank 5. The number of through holes 322 is equal to the number of storage tanks 5. When the number of the via holes 322 is plural, the plural via holes 322 are axially arranged. Each of the via holes 322 communicates with one of the connection chambers 51.

The configuration of the inner cavity of the can 32 includes, but is not limited to, the following embodiments.

Optionally, referring to fig. 3 and 4, the liquid-taking surface 321 is located at the end of the sidewall of the inner cavity of the tank 32. In other words, the liquid-extracting surface 321 is connected to the top cover 33. The radial dimension of the liquid taking surface 321 is larger than the radial dimension on the side of the liquid taking surface 321 in the axial direction. Specifically, the pickup surface 321 can be near the bottom of the can 32 or away from the bottom of the can 32. In other words, the inner cavity of the can 32 is tapered in a forward or reverse direction (see fig. 5).

Further, referring to fig. 1, the top opening of the can 32 may have a polygonal shape, for example, N-shaped edges such as 5, 6, 7, 8, 9, 10, 11. In one embodiment, the bottom surface of the can 32 is circular and the top opening of the can 32 has a perimeter that is an 8-sided shape. The tank body 32 is a frustum centrifugal tank 3 which is transited from a bottom circle to an 8-edge tank top. In another embodiment, the bottom surface of the can 32 is an 8-sided polygon, and the top opening of the can 32 has a surrounding edge with an 8-sided polygon. The tank 32 is a frustum centrifugal tank 3 with the bottom of the tank 32 and the top of the tank 32 both in an 8-edge shape. In still another embodiment, the top opening of the can 32 has a circular peripheral edge, and the bottom surface of the can 32 may have a circular or polygonal shape. Referring to fig. 6 to 8, the top opening of the can 32 has a circular peripheral edge, and the bottom of the can 32 has a circular bottom.

Optionally, referring to fig. 9, the liquid-taking surface 321 is located in the middle of the sidewall of the inner cavity of the tank 32. The radial dimension of the liquid taking surface 321 is larger than the radial dimension of one side of the liquid taking surface 321 in the axial direction and equal to the radial dimension of the other side of the liquid taking surface 321. Specifically, a portion of the sidewall of the inner cavity of the can 32 is tapered, and is defined as a tapered wall 323; the other portion is cylindrical and defines a cylindrical wall 324. The liquid surface 321 is the cylindrical wall surface 324 or a portion of the cylindrical wall surface 324 near the tapered wall surface 323. In one embodiment, tapered wall 323 connects between cylindrical wall 324 and the bottom of can 32, where can 32 has a tapered can 32 below and a cylindrical can 32 above. Further, the dimension of the cylindrical can 32 in the axial direction is much smaller than the dimension of the conical can 32 in the axial direction. For example, the dimension of the cylindrical can 32 in the axial direction is slightly larger than the dimension of the via hole 322. In another embodiment, a cylindrical wall 324 is connected between the conical wall 323 and the bottom of the can 32, in which case the can 32 is a conical can 32 above and a cylindrical can 32 below. Further, the dimension of the cylindrical can 32 in the axial direction is much smaller than the dimension of the conical can 32 in the axial direction. For example, the dimension of the cylindrical can 32 in the axial direction is slightly larger than the dimension of the via hole 322.

Optionally, referring to fig. 10, the liquid-taking surface 321 is located in the middle of the sidewall of the inner cavity of the tank 32. The radial dimension of the liquid taking surface 321 is larger than the radial dimensions of both sides of the liquid taking surface 321 in the axial direction. Specifically, the upper and lower portions of the sidewall of the inner cavity of the can 32 are both tapered, the middle portion of the sidewall of the inner cavity of the can 32 is cylindrical, and the liquid-taking surface 321 is located in the middle portion of the sidewall of the inner cavity of the can 32. Further, the dimension of the tapered portion of the can 32 in the axial direction is much smaller than the dimension of the cylindrical portion of the can 32 in the axial direction. For example, the dimension of the tapered portion of the can 32 in the axial direction is slightly larger than the dimension of the via hole 322.

The above embodiments are the embodiments of the cavity structure of the can 32 listed in the examples of the present application, and of course, the embodiments of the cavity structure of the can 32 are not limited thereto, and those skilled in the art can also form the embodiments by stacking and deforming the embodiments provided in the present application, which also belong to the scope of protection of the present application.

In the above embodiments, the radial dimension of the liquid-taking surface 321 of the tank body 32 of the centrifugal tank 3 is designed to be the largest, so that the centrifugal force at the liquid-taking surface 321 is the largest; under the centrifugal force, the outer layer cells with the highest specific gravity are distributed along the inclined wall of the centrifugal tank 3 and concentrated towards the liquid taking surface 321 with the high centrifugal force, and when the liquid taking surface 321 is positioned at the top of the tank body 32, the outer layer cells with the highest specific gravity finally form a ring shape at the top of the tank body 32; the cells with the lower specific gravity form a ring along the inner circle of the cells on the outer layer; analogize in turn, blood is under the centrifugal force, is the multilayer cyclic annular at getting the laminating of liquid level 321, gets liquid level 321 and is equipped with via hole 322, opens through control switch subassembly 7 to make via hole 322 and storage chamber 52 of storage jar 5 switch on, and then make the outer cell of the biggest proportion automatically enter storage chamber 52 of storage jar 5 under the centrifugal force, this mode need not the suction, laborsaving, economizes the structure.

Referring to fig. 1, the centrifugal separation apparatus 100 further includes a light source 21, a detection camera 22 and a photoelectric sensor (not shown) disposed on the top cover 33. The light source 21, the detection camera 22 and the photoelectric sensor may be disposed inside the top cover 33. The light source 21 is used to illuminate the interior chamber 31 of the centrifuge cup 3. The light source 21 is controlled to operate when the centrifugal separation apparatus 100 is rotated. The photoelectric sensor is configured to send a trigger signal to the detection camera 22 when detecting that the monomer liquid is separated from the mixed liquid, so as to trigger the detection camera 22 to start detection. For example, the mixed solution is blood, the photoelectric sensor can be used to detect color, and when the photoelectric sensor detects that the blood is color-layered, a trigger signal is sent to the detection camera 22 to trigger the detection camera 22 to start detection. The detection camera 22 is used to detect whether the mixed liquor is in a completely layered state. The mixed solution is in a completely layered state, which means that the monomer liquids having different specific gravities are in a stable layered state.

The inner surface of the top cover 33 is also provided with a scale (not shown) which will appear in the picture taken by the inspection camera 22. By referring to the scale, the volume of the monomer fluid of the different layers can be calculated.

Referring to fig. 1 and 2, a top cover 33 covers the top of the can 32. The storage tank 5 may be provided on the top cover 33. In other embodiments, the storage tank 5 may be provided on the outer side of the tank 32. In the present embodiment, the storage tank 5 is disposed on the top cover 33, and the liquid level 321 is disposed on the top of the tank 32.

The storage tank 5 is plural in number. A plurality of storage tanks 5 are provided along the periphery of the top cover 33.

Referring to fig. 3 and 4, the connection cavity 51 of the storage tank 5 includes a first cavity 511 and a second cavity 512, which extend in a crossing direction and are in communication with each other. The first chamber 511 communicates with the inner chamber 31 of the centrifuge tank 3. The second chamber 512 communicates with the storage chamber 52. In particular, the connection chamber 51 is "L" shaped. The first cavity 511 is a horizontally extending cavity, and the second cavity 512 is a vertically extending cavity. Wherein the first cavity 511 and the second cavity 512 are located at the side of the top of the can 32. The storage chamber 52 of the storage tank 5 is located on the top cover 33 or on the periphery of the top cover 33. It will be appreciated that the storage tank 5 will rotate with the centrifuge tank 3.

Referring to fig. 4, the storage tank 5 further includes a receiving cavity 53. The accommodating cavity 53 is located on a side of the second cavity 512 away from the storage cavity 52 and is in conduction with the second cavity 512 to form a slide for the sliding of the switch assembly 7. The housing chamber 53 is a vertically extending chamber. The slide extends in a vertical direction. The outer peripheral wall of the switch component 7 is in sliding connection with the inner peripheral wall of the slide way and is in clearance fit with the inner peripheral wall of the slide way.

Referring to fig. 1, the centrifugal separation apparatus 100 further includes a driving member 4. The driving member 4 is connected to the switch assembly 7 by means including, but not limited to, electrical connection, magnetic connection, and direct contact connection. The driving member 4 is used for driving the switch assembly 7 to slide along the slideway, so that the switch assembly 7 blocks the opening of the first cavity 511 or the first cavity 511 is communicated with the storage cavity 52. The driving means of the driving member 4 includes, but is not limited to, a motor driving means, an electromagnetic driving means, and the like.

Referring to fig. 2 to 4, the switch assembly 7 includes a sliding column 71 and an elastic member 72 sleeved on the sliding column 71. The outer peripheral wall of the first end of the sliding column 71 is in sliding connection and clearance fit with the inner peripheral wall of the slideway. The second end of the slide column 71 is elastically abutted with the tank 5 in the sliding direction by an elastic member 72 outside the tank 5. In particular, the switch assembly 7 may be an electric valve or a solenoid valve. The sliding post 71 is a valve spool, and the elastic member 72 is a valve spring.

Optionally, referring to fig. 1, the sliding column 71 is made of a magnetic material. The driving member 4 includes a magnetic member 41 disposed opposite to the sliding column 71. At least one of the sliding column 71 and the magnetic member 41 is an electromagnet. The sliding column 71 is adapted to slide under the magnetic attraction or repulsion of the magnetic member 41.

In the first embodiment, the sliding column 71 is a permanent magnet or the sliding column 71 is made of a magnetically permeable material, and the magnetic member 41 is an electromagnet. The magnetic member 41 may be provided on a side of the sliding column 71 facing away from the storage tank 5. Referring to fig. 1, the centrifugal separator 100 further includes an electromagnet base 42. The electromagnet base 42 is annularly disposed around the centrifuge tank 3 and spaced from the centrifuge tank 3. The magnetic member 41 is provided on the electromagnet base 42. The magnetic member 41 has a ring shape. The magnetic member 41 is enclosed around the outer periphery of the centrifuge tank 3. The magnetic member 41 includes a plurality of magnet blocks arranged in a ring shape in sequence to facilitate assembly.

When the detection camera 22 detects that the mixed liquid is in a completely layered state, the controller of the centrifugal separation device 100 controls the magnetic member 41 to magnetically attract the sliding column 71 of one of the storage tanks 5, the sliding column 71 moves toward the magnetic member 41 (i.e., moves downward) under the magnetic attraction force until the first cavity 511 is communicated with the second cavity 512, the elastic member 72 is compressed, thereby leading the inner cavity 31 of the centrifugal tank 3 to be communicated with the storage cavity 52 of the storage tank 5, and at the moment, the monomer liquid with the highest specific gravity automatically enters the storage cavity 52 under the centrifugal force, when the monomer liquid with the highest specific gravity completely enters the storage cavity 52, the controller controls the magnetic member 41 to magnetically repel the sliding column 71 of the storage tank 5, and the sliding column 71 moves away from the magnetic member 41 under the magnetic attraction force (i.e., moves upward) until the separation between the inner cavity 31 of the centrifuge tank 3 and the storage cavity 52 of the storage tank 5 is realized.

By arranging the magnetic member 41 in a ring shape, magnetic force can be generated simultaneously for the plurality of sliding columns 71, and the opening and closing of the inner chamber 31 and the storage chamber 52 of the centrifuge tank 3 can be controlled.

Of course, in other embodiments, the magnetic member 41 may be disposed on the storage tank 5 and opposite to the sliding column 71. Since the magnetic member 41 is rotated with the centrifugation tank 3, a conductive slip ring may be installed on the electrical connection line between the magnetic member 41 and the power source to prevent the electrical connection line from being twisted during the rotation. In this embodiment, the electromagnet base 42 is not required, simplifying the overall construction of the centrifugal separation apparatus 100 and reducing the overall volume of the centrifugal separation apparatus 100; meanwhile, the distance between the sliding column 71 and the magnetic member 41 is relatively small, and the electromagnetic control efficiency is improved.

In the second embodiment, different from the first embodiment, the slide column 71 is an electromagnet, and the magnetic member 41 is an electromagnet. The positions and structures of the sliding column 71 and the magnetic member 41 can refer to the first embodiment, and are not described herein again.

In the third embodiment, unlike the first embodiment, the slide column 71 is an electromagnet, and the magnetic material 41 is a permanent magnet or a material that is permeable to the magnetic material 41. The positions and structures of the sliding column 71 and the magnetic member 41 can refer to the first embodiment, and are not described herein again.

In other embodiments, the material of the sliding column 71 may not be magnetic. The driving member 4 includes a pair of oppositely disposed magnetic members, one of which is located at a first end or a second end of the sliding column 71.

In the present embodiment, the slide column 71 is specifically described as a permanent magnet.

Optionally, referring to fig. 4, the first end of the sliding column 71 has a through hole 711. The through hole 711 has an L-shape. The first opening 711a of the through hole 711 faces and communicates with the second cavity 512. The second opening 711b of the through hole 711 is provided in the outer peripheral wall of the slide column 71. When the sliding column 71 slides to the second opening 711b to communicate with the first cavity 511 under the action of the driving member 4, the storage cavity 52 communicates with the inner cavity 31 of the centrifuge tank 3. When the sliding column 71 slides under the action of the driving member 4 until the peripheral wall of the sliding column 71 closes the opening of the first cavity 511, the storage chamber 52 is disconnected from the inner cavity 31 of the centrifuge tank 3.

Alternatively, the sliding column 71 may be a solid cylindrical structure. When the sliding post 71 slides away from the opening of the first chamber 511 by the driving member 4, the storage chamber 52 is in communication with the inner chamber 31 of the centrifuge tank 3. When the sliding column 71 slides under the action of the driving member 4 until the peripheral wall of the sliding column 71 closes the opening of the first cavity 511, the storage chamber 52 is disconnected from the inner cavity 31 of the centrifuge tank 3.

The storage tank 5 is plural in number. The south and north poles of the sliding columns 71 to which all the storage tanks 5 are connected are arranged in the same direction. For example, all the sliding columns 71 have their south poles facing downward and their north poles facing upward. Alternatively, all the slide columns 71 have their north poles facing downward and their south poles facing upward.

The plurality of storage tanks 5 are divided into at least two groups, south poles and north poles of the sliding columns 71 connected with the storage tanks 5 in the same group are arranged in the same direction, and south poles and north poles of the sliding columns 71 connected with the storage tanks 5 in different groups are arranged in the opposite direction. For example, the south poles of the sliding columns 71 to which the storage tanks 5 of one group are connected face downward, and the south poles of the sliding columns 71 to which the storage tanks 5 of the other group are connected face upward. So that the poles of the sliding columns 71 of different groups are opposite, the opening and closing of the storage tanks 5 of different groups can be controlled alternately. In other words, the magnetic member 41 can be controlled to generate magnetic attraction to the sliding column 71 of one set of storage tanks 5, and the magnetic member 41 can be controlled to generate magnetic repulsion to the sliding column 71 of the other set of storage tanks 5, so that the one set of storage tanks 5 can be controlled to be communicated with the centrifugal tank 3, and the other set of storage tanks 5 can be controlled to be disconnected from the centrifugal tank 3, so that different types of monomer liquids can be stored in the two sets of storage tanks 5 respectively, thereby preventing the monomer liquids between the different types from interfering with each other and improving the purity of the monomer liquid required to be extracted.

Referring to fig. 6 to 8, the set of storage tanks 5 includes a plurality of first storage tanks 54. The plurality of first storage tanks 54 are evenly arranged around the circumferential direction of the centrifuge tank 3. The other set of storage tanks 5 comprises a plurality of second storage tanks 55. The plurality of second storage tanks 55 are evenly arranged around the circumferential direction of the centrifugal tank 3. Further, the outer peripheral surface of the first storage tank 54 and the outer peripheral surface of the second storage tank 55 are connected to form a dual-chamber storage tank 56. It should be noted that the storage chamber 52 of the first storage tank 54 is not communicated with the storage chamber 52 of the second storage tank 55. The pipette 9 communicated with the first storage tank 54 and the pipette 9 communicated with the second storage tank 55 are converged into one pipe, so that the first storage tank 54 and the second storage tank 55 can be removed by using one pipette 9. It should be noted that the first storage tank 54 and the second storage tank 55 store the monomer liquid at different time periods, respectively.

Referring to fig. 11, the top cover 33 has a center hole 331 formed at the geometric center thereof. The middle shaft hole 331 communicates the inner cavity of the centrifugal tank 3 with the outside air.

Referring to fig. 11, the liquid guiding tube 1 includes a first liquid guiding tube 11, a rotary connecting tube 12 and a second liquid guiding tube 13 which are sequentially connected. The first liquid guide tube 11 is arranged outside the centrifuge tank 3. One end of the first liquid guide tube 11 is arranged outside the centrifugal separation device 100, and the other end of the first liquid guide tube 11 is connected to one end of the rotary connecting tube 12 extending out of the centrifugal tank 3. The rotary connection pipe 12 is inserted into the center shaft hole 331. A second liquid conduit 13 is arranged in the centrifuge tank 3. One end of the second liquid guide pipe 13 is connected with the other end (the end extending into the centrifuge tank 3) of the rotary connecting pipe 12, and the other end of the second liquid guide pipe 13 is close to the bottom surface of the tank body 32. In this way, the mixed liquid can be introduced into the cavity 31 of the centrifuge tank 3 through the first liquid guide tube 11, the rotary joint tube 12, and the second liquid guide tube 13.

Referring to fig. 11, the centrifugal separation device 100 further includes a first bearing 61 and a first seal ring 62. The first bearing 61 is provided between the outer periphery of the rotation connecting pipe 12 and the inner wall of the middle shaft hole 331. The first seal ring 62 is disposed coaxially with the first bearing 61, and the first seal ring 62 is disposed between the outer periphery of the rotary joint pipe 12 and the inner wall of the center shaft hole 331.

In the present embodiment, the storage tank 5 is provided in the top cover 33 as an example. Referring to fig. 11, the storage tank 5 is plural in number. A plurality of storage tanks 5 are provided along the periphery of the top cover 33. The number of pipettes 9 is plural. A pipette 9 communicates with at least one storage tank 5. In other words, one pipette 9 may communicate with one storage tank 5 or with a plurality of storage tanks 5. One ends of the plurality of pipettes 9 are connected to the storage tank 5, and the other ends of the plurality of pipettes 9 extend in the radial direction of the top cover 33 and surround the periphery of the end of the rotary connection pipe 12 extending out of the top cover 33, so that the other ends of the plurality of pipettes 9 are collected to form a convex end 69.

Referring to fig. 11, the centrifugal separation apparatus 100 further includes a sleeve 63, a cover 64, a second bearing 65, a second sealing ring 66, a third sealing ring 67, and a third liquid guiding tube 68. One end of the sleeve 63 is sleeved on the outer periphery of the convex end 69 so as to enable the plurality of pipettes 9 to be communicated with the inner cavity of the sleeve 63. A second bearing 65 is provided between the male end 69 and the sleeve 63. The second seal ring 66 and the third seal ring 67 are provided coaxially with the second bearing 65. The second seal ring 66 and the third seal ring 67 are both connected between the convex end 69 and the sleeve 63 in a sealing manner. The second seal ring 66 and the third seal ring 67 are provided on opposite sides of the second bearing 65, respectively. The first liquid guide tube 11 passes through the sleeve 63 from outside the centrifugal separation device 100 to be connected to the rotary connection tube 12. A cap 64 covers the other end of the sleeve 63. One end of the third liquid guide tube 68 is communicated with the inner cavity of the sleeve 63, and the other end of the third liquid guide tube 68 is communicated with the outside of the centrifugal separation device 100.

The storage chamber 52, the pipette 9, the sleeve 63 and the third liquid guide tube 68 form a communication passage for guiding out the monomer liquid in the storage chamber 52.

Referring to fig. 12, the embodiment of the present application further provides a method for separating and culturing a mixed solution, which is applied to a centrifugal separation apparatus 100. Referring to fig. 1 to 11, the centrifugal separation apparatus 100 includes at least one liquid guiding pipe 1, a centrifugal tank 3, at least one storage tank 5, at least one switch assembly 7, and at least one pipette 9. The inner cavity 31 of the centrifugal tank 3 is communicated with one end of the liquid guide pipeline 1 and used for centrifuging the mixed liquid. The storage tank 5 is connected to the centrifuge tank 3, the storage tank 5 has a connecting chamber 51 and a storage chamber 52, and the connecting chamber 51 is connected between the storage chamber 52 and the inner chamber 31 of the centrifuge tank 3. The switch assembly 7 is connected to the storage tank 5. One end of the pipette 9 opens the storage chamber 52. Other structural features of the centrifugal separation device 100 can be found in any of the above embodiments, and will not be described again.

The mixed solution separation and culture method comprises the following steps.

Step 100: the mixed solution is injected into a centrifuge tank 3 through a liquid guide pipe 1.

Specifically, the catheter tube 1 includes a first catheter tube 11 and a second catheter tube 13. One end of the first liquid guide tube 11 penetrates the sleeve 63, and the other end of the first liquid guide tube 11 is communicated with one end of the rotary connecting tube 12. The other end of the rotary connecting tube 12 is communicated with a second liquid guide tube 13. The second liquid guide tube 13 is arranged in the centrifuge tank 3 and close to the bottom surface of the centrifuge tank 3. The mixed liquid can be injected into the centrifuge tank 3 through the first liquid guide tube 11, the rotary connecting tube 12 and the second liquid guide tube 13.

Step 200: the centrifuge tank 3 is driven to rotate.

Specifically, the centrifugal tank 3 can be connected with a high-speed motor, and the motor drives the centrifugal tank 3 to rotate at a high speed, so that multiple monomer liquids in the mixed liquid form multiple layered monomer liquids due to different specific gravities.

Step 300: detecting whether the mixed liquid forms various layered monomer liquids along the inner side wall of the centrifugal tank 3, and if so, determining the layer of the target monomer liquid in the multilayer monomer liquid.

Specifically, whether the monomer liquid is separated from the mixed liquid is detected by the photoelectric sensor, and when the monomer liquid is separated from the mixed liquid, a trigger signal is sent to the detection camera 22, and the detection camera 22 detects whether the mixed liquid is in a completely layered state. The mixed solution is in a completely layered state, which means that the monomer liquids having different specific gravities are in a stable layered state. And the detection camera 22 can determine the layer of the multi-layer monomer liquid where the target monomer liquid is located.

Step 400: the control switch assembly 7 switches on or off the storage cavity 52 and the inner cavity 31 of the centrifugal tank 3, so as to lead out the monomer liquid on the outer layer of the target monomer liquid through the storage tank 5 and the pipette 9, store the target monomer liquid into the storage tank 5, and lead out the monomer liquid on the inner layer of the target monomer liquid out of the centrifugal tank 3 through the liquid guide pipeline 1.

Specifically, when the target monomer liquid level is at the outermost layer, after the mixed liquid is in a completely layered state, the control switch assembly 7 conducts the storage cavity 52 and the inner cavity 31 of the centrifugal tank 3, so that the target monomer liquid automatically enters the storage cavity 52 under the centrifugal force; the remaining mixture in centrifuge tank 3 is then removed via drain 1.

Specifically, when the target monomer liquid level is in the middle layer, after the mixed liquid is in a completely layered state, the control switch assembly 7 conducts the storage cavity 52 and the inner cavity 31 of the centrifugal tank 3, so that the monomer liquid on the outer layer of the target monomer liquid automatically enters the storage cavity 52 under the centrifugal force and is moved out through the pipette 9; the storage cavity 52 and the inner cavity 31 of the centrifugal tank 3 are conducted through the switch component 7, and the target monomer liquid automatically enters the storage cavity 52 under the centrifugal force; the remaining mixture in centrifuge tank 3 is then removed via drain 1.

Step 500: the target monomer liquid is transferred to the centrifugal tank 3.

After the target monomer liquid automatically enters the storage chamber 52 under centrifugal force, the target monomer liquid is transferred to the centrifugal tank 3.

Specifically, when the liquid level of the target monomer is at the innermost layer, after the mixed liquid is in a completely layered state, the control switch assembly 7 conducts the storage cavity 52 and the inner cavity 31 of the centrifugal tank 3, so that the monomer liquid at the outer layer of the target monomer liquid automatically enters the storage cavity 52 under the centrifugal force and is moved out through the pipette 9; the target monomer liquid was retained in the centrifuge tank 3.

Step 600: the culture solution is injected into the centrifuge tank 3 through the drainage tube 1 to culture the objective monomer solution.

When the mixed solution is a blood sample, the blood sample separation and culture method includes the following steps.

(1) Injecting cell separation liquid through the first liquid guide pipe 11 and the second liquid guide pipe 13, starting to rotate the centrifugal tank 3, and slowly adding cell suspension to be separated after the cell separation liquid is uniformly distributed along the tank wall at a certain rotating speed.

(2) The centrifugal tank 3 is rotated continuously at a certain acceleration, the mixed liquid is layered according to the specific gravity under the action of centrifugal force, and the cell layer with the highest specific gravity is finally distributed at the upper end of the centrifugal tank 3 and is annularly concentrated.

(3) The detection light source 21 at the top of the centrifuge tank 3 irradiates the liquid in the centrifuge tank 3 for layering, the layering detection camera 22 identifies, the separation state is judged by the visual detection system, and the volume of each layering can be calculated according to the scale of the top cover 33 of the centrifuge tank 3.

(4) When the blood sample reaches a stable and satisfactory layering state according to the specific gravity, the electromagnet base 42 is electrified, the elastic piece 72 is compressed, the sliding column 71 rises, the first storage tank 54 in the double-cavity storage tank is controlled to be communicated with the centrifugal tank 3, and the outer-layer monomer liquid enters the first storage tank 54;

(5) after the cell layer with the largest specific gravity completely enters the first storage tank 54, controlling the sliding column 71 to descend, and disconnecting the first storage tank 54 from the centrifugal tank 3; during this process the spin pot 3 is rotated to maintain sufficient centrifugal force to maintain the stratification state stable. The cell liquid in the first storage tank 54 is drawn by the pipette 9 and the third liquid guide 68.

(6) The cell layer of lower specific gravity will be collected in a ring shape at the top of the centrifugal tank 3, and the valve of the second storage tank 55 in the dual-chamber storage tank can be opened, and the cell layer of lower specific gravity will be introduced into the second storage tank 55.

(7) The cell culture solution is injected into the centrifugal tank 3 through the first liquid guide pipe 11 and is separated to the first storage tank through rotation, so that the centrifugal tank 3 is cleaned, the impurity cell residue in the centrifugal tank 3 is avoided, and the purity of the next cell culture is kept.

(8) Assuming that the cells in the second storage tank 55 are the target cells, the electromagnet base 42 is energized, the elastic member 72 of the second storage tank 55 is compressed, the sliding column 71 of the second storage tank 55 is raised, the conduction between the second storage tank 55 and the centrifugal tank 3 is controlled, and the target cells in the second storage tank 55 are flushed back to the centrifugal tank 3 by the cell culture fluid, so that the residue of the target cells in the second storage tank 55 is reduced, thereby ensuring a satisfactory target cell yield.

When the target cells are cultured in the centrifugal tank 3 for a certain period of time and the cell culture solution needs to be replaced (replenished):

(9) the spin centrifuge tank 3 is activated to separate the target cells into the second storage tank 55 and the waste liquid is removed through the drain conduit 1.

(10) Repeating the steps (8) - (9) to replace (supplement) the cell culture solution as required until the cell culture is completed, and washing and recovering the cell preparation.

The invention can complete the cell separation and culture process for many times according to the requirement under the condition of no manual intervention in the whole process in the same device.

In the centrifugal separation device 100 provided by the embodiment of the application, the centrifugal tank 3 is designed to be conical, the layered state of the mixed solution is changed, and the monomer liquid with high specific gravity is concentrated at the vertex angle of the tank body 32 and distributed annularly; by designing the double-cavity storage tank with the control switch assembly 7 at the outer edge of the top of the centrifugal tank 3, the monomer liquid separated to the top end of the tank body 32 can be automatically extracted into the double-cavity storage tank by utilizing the principle that the monomer liquid with high specific gravity is concentrated at the top corner end of the tank body 32 by centrifugal force; after the target cells are obtained by separation, the target cells are temporarily placed in a storage tank, a centrifugal tank 3 is washed, culture solution is injected, the target cells flow back to the centrifugal tank 3 from the storage tank for culture, and the chance that the target cells are polluted by the environment in the transfer culture process can be eliminated, so that one-tank integrated separation and culture can be realized; the double-cavity storage tank is provided with a pipette 9 for extracting the monomer liquid in the double-cavity storage tank to a specified position; 3 central seal structure of centrifuging tank adopts central double bearing structure, and the terminal surface adopts the sealing washer sealed about the bearing, and whole 3 inside complete sealings of centrifuging tank are in aseptic environment, get rid of external environmental pollution's chance, are connected with two bearings through rotatory switching pipe, realize centrifuging tank 3 and access & exit passageway and be connected.

The foregoing is a partial description of the present application, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present application, and these modifications and decorations are also regarded as the protection scope of the present application.

Claims

1. A centrifugal separation device, comprising:

a centrifuge tank for centrifuging a mixed liquor, the mixed liquor comprising at least two monomer fluids;

the storage tank is connected with the centrifugal tank, the storage tank is provided with a connecting cavity and a storage cavity, the side wall of the inner cavity of the centrifugal tank comprises an annular liquid taking surface, the liquid taking surface is provided with at least one conducting hole, the conducting hole is communicated with the connecting cavity, the connecting cavity is communicated between the storage cavity and the inner cavity of the centrifugal tank, and the storage cavity is used for storing monomer liquid separated from the mixed liquid;

2. The centrifugal separation device of claim 1, wherein the connecting chamber comprises a first chamber and a second chamber which extend in a crossed manner and are communicated with each other, the first chamber communicates with the inner chamber of the centrifugal tank, and the second chamber communicates with the storage chamber; the storage jar is still including acceping the chamber, it is located to accept the chamber the second cavity is kept away from one side of storage chamber and with the second cavity switches on, in order to form and be used for the gliding slide of switch module, switch module's periphery wall with the internal perisporium clearance fit of slide.

3. The centrifugal separation device of claim 2, further comprising a driving member connected to the switch assembly, wherein the driving member is configured to drive the switch assembly to slide along the slide to block the opening of the first chamber or to communicate the first chamber with the storage chamber.

4. The centrifugal separation device of claim 3, wherein the switch assembly comprises a sliding column and an elastic member sleeved on the periphery of the sliding column, the outer circumferential wall of the first end of the sliding column is slidably connected with the inner circumferential wall of the slideway and is in clearance fit with the inner circumferential wall of the slideway, and the second end of the sliding column is elastically abutted with the storage tank outside the storage tank along the sliding direction through the elastic member; the driving piece comprises a magnetic piece which is arranged opposite to the sliding column and has a magnetic effect with the sliding column, the magnetic piece is arranged on one side of the sliding column, which is far away from the storage cavity, and is annular, and the magnetic piece is arranged around the periphery of the centrifugal tank and is arranged at intervals with the centrifugal tank; at least one of the sliding column and the magnetic part is an electromagnet, and the sliding column is used for sliding under the magnetic attraction force or the repulsion force of the magnetic part.

5. The centrifugal separation apparatus as claimed in claim 4, wherein said plurality of storage tanks are divided into at least two groups, the south and north poles of said sliding columns connected to said storage tanks of the same group are arranged in the same direction, and the south and north poles of said sliding columns connected to said storage tanks of different groups are arranged in the opposite direction; it is a set of the storage jar includes a plurality of first storage jar, and is a plurality of first storage jar winds the circumference of centrifugation jar is evenly arranged, and another group the storage jar includes a plurality of second storage jar, and is a plurality of the second storage jar winds the circumference of centrifugation jar is evenly arranged, the outer peripheral face of first storage jar with the outer peripheral face of second storage jar is connected, and communicates first storage jar the pipette and the intercommunication the second storage jar the pipette assembles into a pipeline.

6. The centrifugal separation device of claim 4, wherein the first end of the sliding column has a through hole, a first opening of the through hole faces and communicates with the second cavity, and a second opening of the through hole is arranged on the outer peripheral wall of the sliding column, and when the sliding column slides to the second opening to communicate with the first cavity under the action of the magnetic member, the storage cavity communicates with the inner cavity of the centrifugal tank; when the sliding column slides to the outer peripheral wall of the sliding column under the action of the magnetic part to block the opening of the first cavity, the storage cavity is disconnected with the inner cavity of the centrifugal tank.

7. The centrifugal separation device as claimed in any one of claims 1 to 6, wherein the centrifugal tank comprises a tank body and a top cover covering the tank body, and a central shaft hole is formed in the geometric center of the top cover; the liquid guide pipeline comprises a first liquid guide pipe, a rotary connecting pipe and a second liquid guide pipe which are sequentially communicated, one end of the first liquid guide pipe, which is far away from the rotary connecting pipe, is arranged outside the centrifugal separation device, the rotary connecting pipe is arranged in the middle shaft hole in a penetrating manner, one end of the second liquid guide pipe, which is far away from the rotary connecting pipe, is close to the bottom surface of the tank body, the centrifugal separation device further comprises a first bearing, and the first bearing is arranged between the periphery of the rotary connecting pipe and the inner wall of the middle shaft hole;

the storage tank is in a plurality of numbers, and the storage tanks are arranged along the periphery of the top cover; the number of the pipettes is multiple, one pipette is communicated with at least one storage tank, and the pipettes extend along the radial direction of the top cover and are arranged around the periphery of the end part of the rotary connecting pipe extending out of the top cover to form an outer convex end;

the centrifugal separation device further comprises a sleeve, a sealing cover, a second bearing and a third liquid guide pipe, wherein one end of the sleeve is sleeved on the periphery of the convex end so as to enable the plurality of pipettes to be communicated with the inner cavity of the sleeve; the second bearing is arranged between the convex end and the sleeve; the first liquid guide pipe penetrates through the sleeve pipe from the outside of the centrifugal separation device to be connected to the rotary connecting pipe; the sealing cover covers the other end of the sleeve; one end of the third liquid guide pipe is communicated with the inner cavity of the sleeve, and the other end of the third liquid guide pipe is communicated with the outside of the centrifugal separation device.

8. The centrifuge of claim 7, further comprising a light source, a photosensor and a detection camera on the top cover, wherein the light source is configured to illuminate the centrifuge tank, and the photosensor is configured to send a trigger signal to the detection camera to trigger the detection camera to start detection when the separation of the monomer liquid from the mixed liquid is detected; the detection camera is used for detecting whether the mixed liquid is in a complete layering state or not.

9. The centrifugal separation device of claim 7, wherein the extraction surface is located at an end of a sidewall of the tank interior, the extraction surface having a radial dimension in the axial direction that is greater than a radial dimension of a side of the extraction surface; or the radial dimension of the liquid taking surface is larger than the radial dimension of one side of the liquid taking surface in the axial direction and is equal to the radial dimension of the other side of the liquid taking surface; or the radial dimension of the liquid taking surface is larger than the radial dimensions of two sides of the liquid taking surface in the axial direction.

10. A mixed liquid separation and culture method is applied to a centrifugal separation device and is characterized in that the centrifugal separation device comprises at least one liquid guide pipeline, a centrifugal tank, at least one storage tank, at least one switch component and at least one pipette, wherein an inner cavity of the centrifugal tank is communicated with one end of the liquid guide pipeline and is used for centrifuging the mixed liquid; the storage tank is connected with the centrifugal tank, the storage tank is provided with a connecting cavity and a storage cavity, and the connecting cavity is communicated between the storage cavity and an inner cavity of the centrifugal tank; the switch assembly is connected with the storage tank; one end of the pipette is communicated with the storage cavity; the method comprises the following steps:

driving the centrifugal tank to rotate;

delivering the target monomer fluid to the centrifuge tank;