CN216837995U

CN216837995U - Integrated nucleic acid rapid-extraction test tube and rapid-extraction and detection device

Info

Publication number: CN216837995U
Application number: CN202121181954.9U
Authority: CN
Inventors: 王德明; 周杰锋
Original assignee: Ningbo Kangcheng Deno Biomedical Co ltd
Current assignee: Ningbo Kangcheng Deno Biomedical Co ltd
Priority date: 2021-05-28
Filing date: 2021-05-28
Publication date: 2022-06-28
Anticipated expiration: 2031-05-28

Abstract

The utility model relates to a nucleic acid draws and detection area, especially relates to a test tube is carried soon to integration nucleic acid. The test tube comprises a main tube; the inner cavity of the main pipe is sequentially provided with a cracking area, a washing area and an elution area from top to bottom; hydrophobic sealing layers are respectively arranged between the cracking zone and the washing zone and between the washing zone and the elution zone for separation, and magnetic bead washing liquid and nucleic acid eluent are respectively arranged in the washing zone and the elution zone; the inner cavity of the main pipe is also provided with a magnetic track extending from the cracking area to the elution area, one end of the magnetic track is closed, and the other end of the magnetic track is provided with an opening for the magnetic rod to extend into. The test tube is provided with a magnetic track inside the main tube, and the magnetic rod is inserted into an inner hole of the magnetic track to drive the magnetic bead to be attached to the outer wall of the magnetic track and move along with the magnetic rod; therefore, the magnetic beads can move downwards without being limited by the number of magnetic bead channels, a large number of magnetic beads are driven at one time, and the nucleic acid extraction efficiency is improved; meanwhile, the magnetic beads are uniformly distributed on the outer wall of the magnetic track with larger area and are not stacked, so that the magnetic beads are more stable in movement.

Description

Integrated nucleic acid rapid-extraction test tube and rapid-extraction and detection device

Technical Field

The utility model relates to a nucleic acid extraction and detection area especially relate to a test tube is carried soon to integration nucleic acid.

Background

Along with the popularization of gene detection, personalized drug delivery, prenatal diagnosis and the like, the traditional DNA extraction method is more and more obviously limited today when high flux and automation are pursued in various fields of the biological industry. Because the magnetic bead method for extracting nucleic acid can realize automatic extraction and large-scale operation, and has simple operation and short time, the magnetic bead method for extracting nucleic acid is more and more emphasized.

The Chinese invention patent application (publication No. CN108796038B, published: 20191018) discloses a nucleic acid integrated detection method and a detection reagent tube, wherein a plurality of separating plugs which are arranged up and down are arranged in a main tube, and a liquid-phase or solid-phase hydrophobic layer is arranged on each separating plug, so that lysate, cleaning liquid and reaction liquid in the detection reagent tube are isolated; adding a sample into a lysis solution for uniform mixing and lysis, extracting nucleic acid in the sample by using nano magnetic beads, driving the nano magnetic beads to carry the nucleic acid to sequentially pass through each hydrophobic layer along a magnetic bead channel on the inner wall of a detection reagent tube by using an external magnetic body to enter cleaning solution and reaction solution, and realizing the cleaning and amplification of the nucleic acid, wherein a biological reagent required by the reaction solution is stored in a separation plug above the reaction solution, and finally, external equipment realizes the detection of the sample nucleic acid through optical detection, thereby realizing multiple steps of nucleic acid extraction, cleaning and amplification reaction in the same detection reagent tube. The utility model has the characteristics of can reduce the detection error and reduce the operation degree of difficulty.

The prior art has the following defects: the magnetic bead channel sets up and is being responsible for the inner wall and quantity less, and magnetic bead channel width is narrower, and the magnetic rod is less from the magnetic bead quantity that is responsible for the outer wall and once drives to cause nucleic acid extraction efficiency to be lower. Meanwhile, the width of the magnetic bead channel is narrow, so that the magnetic beads can be easily stacked and cannot be uniformly distributed on the inner wall of the magnetic bead channel, and the magnetic beads are not stable in movement.

Disclosure of Invention

The utility model aims at: aiming at the problems, the magnetic track is arranged in the main pipe, and the magnetic rod is inserted into the inner hole of the magnetic track to drive the magnetic bead to be attached to the outer wall of the magnetic track and move along with the magnetic rod; therefore, the magnetic beads can move downwards without being limited by the number of magnetic bead channels, a large number of magnetic beads are driven at one time, and the nucleic acid extraction efficiency is improved; simultaneously, the magnetic bead evenly distributed does not pile up each other on the great magnetic track outer wall of area for a more steady integration nucleic acid is carried test tube soon during the magnetic bead motion.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

an integrated nucleic acid rapid-extraction test tube, which comprises a main tube; the inner cavity of the main pipe is sequentially provided with a cracking area, a washing area and an elution area from top to bottom; hydrophobic sealing layers are respectively arranged between the cracking zone and the washing zone and between the washing zone and the elution zone for separation, and magnetic bead washing liquid and nucleic acid eluent are respectively arranged in the washing zone and the elution zone; the device is characterized in that the inner cavity of the main pipe is also provided with a magnetic track extending from the cracking area to the elution area, one end of the magnetic track is closed, and the other end of the magnetic track is provided with an opening for the magnetic rod to extend into.

Preferably, the magnetic track bottom end is integrally formed with the main tube and the magnetic track bottom end closes the top end opening, or the magnetic track bottom end is integrally formed with the main tube and the magnetic track bottom end opening top end is closed.

Preferably, the main pipe inner cavity is provided with a first separating plug and a second separating plug; annular gaps are formed among the outer wall of the magnetic track, the first separating plug and the second separating plug; the hydrophobic sealing layers are respectively positioned in the first separating plug and the second separating plug and fill and seal the inner cavity of the main pipe at the position.

Preferably, the hydrophobic sealing layer is a paraffin or liquid-phase hydrophobic layer; the first and second plugs are both of an elastomeric material.

Preferably, the first separating plug comprises a first plug body, a first lower conical surface in an inclined direction, a first upper conical surface in an inclined direction and a first supporting surface in a horizontal direction; the main pipe inner cavity is also provided with a first lower inclined plane in the inclined direction, a first upper inclined plane in the inclined direction and a first step surface in the horizontal direction; the first lower conical surface and the first upper conical surface are respectively attached to the first lower inclined surface and the first upper inclined surface, and the first supporting surface is attached to the first step surface.

Preferably, the main pipe inner cavity is further provided with a first protrusion protruding from the inner surface of the cracking zone inner cavity, and the first protrusion is located above the upper surface of the first partition plug.

Preferably, the second separating plug comprises a second plug body, a second supporting surface positioned in the horizontal direction of the bottom and a second taper surface in the inclined direction; the inner cavity of the main pipe is also provided with a second step surface in the horizontal direction and a second inclined surface in the inclined direction; the second supporting surface is attached to the second step surface, and the second conical surface is attached to the second inclined surface.

Preferably, the main pipe internal cavity is further provided with a second protrusion protruding from the inner surface of the washing zone internal cavity, and the second protrusion is located above the upper surface of the second partition plug.

Preferably, a plurality of partition plates are arranged in the elution area, and the partition plates are respectively connected with the outer wall of the magnetic track and the inner wall of the main pipe cavity at the two radial ends so as to divide the elution area into a plurality of branch pipes.

Preferably, the top end of the main pipe is provided with a first pipe cover, and the first pipe cover is screwed with the external thread of the outer wall of the main pipe through the internal thread; the first pipe cover is provided with a positioning hole which vertically penetrates through the first pipe cover, and the positioning hole is matched with the outer wall of the top opening of the magnetic track.

Preferably, the bottom of the elution area is provided with a second base; the upper end of the second base is provided with a matching hole corresponding to the shape of the outer wall of the elution area, and the matching hole is embedded with the outer wall of the elution area; the lower end of the second base is provided with a fixing hole which is embedded with a corresponding bulge of the main pipe fixing equipment.

Preferably, the elution area is detachably connected with the main pipe; the top height of the second base is aligned with the top height of the elution area, the outer wall of the second base is provided with threads, and the threads of the outer wall of the second base are screwed with the threaded cover.

Preferably, the top end of the main pipe is provided with a first pipe cover, and the first pipe cover comprises a cover body, a storage tank and a sealing cover; the sealing cover is positioned at the top end opening of the storage tank and seals the storage tank; the bottom of the storage tank is provided with a blanking hole, the storage tank is communicated with the main cracking area through the blanking hole, and the blanking hole is internally provided with tinfoil to seal the storage tank.

Preferably, the sealing cover is made of elastic material, and the sealing cover is in interference fit with the storage tank to close the top end opening of the storage tank.

Preferably, the bottom of the elution area is provided with a first base, and a liquid outlet at the bottom of the elution area is open and is communicated with the outside; the first base is provided with a collecting hole and a magnetic track connecting hole, the collecting hole is communicated with a liquid outlet at the bottom of the elution area, and the upper end of the collecting hole is embedded with the outer wall of the liquid outlet at the bottom of the elution area; the opening at the lower end of the magnetic track is communicated with the outside through a magnetic track connecting hole, and the magnetic track connecting hole is matched with the outer wall of the magnetic rod.

Preferably, the inner wall of the cracking zone is vertically and fixedly provided with a plurality of ribs which protrude out of the inner wall of the cracking zone.

Preferably, the first base outer wall is provided with threads, and the threads of the first base outer wall are screwed with the threaded cover.

Further, this application still discloses an integration nucleic acid is carried soon and detection device, include integration nucleic acid carry test tube and bar magnet soon, the bar magnet outer wall cooperatees with the magnetic track hole.

The utility model adopts the above technical scheme's an integration nucleic acid is carried test tube soon's advantage is:

the hydrophobic sealing layer separates cell lysis solution in the lysis zone from magnetic bead washing solution in the washing zone and separates magnetic bead washing solution in the washing zone from nucleic acid eluent in the elution zone; after the magnetic rod is placed into the inner hole of the magnetic track from the top opening of the magnetic track, the magnetic beads in the cracking area are sucked and attached to the outer wall of the magnetic track by using the magnetic force of the magnetic rod; the magnetic bar moves downwards to drive the magnetic beads to move downwards to penetrate through the hydrophobic sealing layer to enter a washing area and an elution area respectively for washing and elution so as to complete the extraction of nucleic acid; in the mode, the magnetic beads are attached to the outer wall of the magnetic track and move along with the magnetic rod, the outer wall of the whole magnetic track can be used for moving the magnetic beads without being limited by the number of magnetic bead channels, a large number of magnetic beads can be driven at one time, and the nucleic acid extraction efficiency is improved; meanwhile, the magnetic beads are uniformly distributed on the outer wall of the magnetic track with larger area and are not stacked, so that the magnetic beads are more stable in movement.

Drawings

Fig. 1 is a schematic structural view of embodiment 1.

Fig. 2 is a schematic structural view of the first separator plug.

Fig. 3 is a schematic structural view of the second separator plug.

Fig. 4 is a schematic structural view of embodiment 2.

Detailed Description

The following describes in detail embodiments of the present invention with reference to the accompanying drawings.

Example 1

An integrated nucleic acid rapid-lift cuvette as shown in FIG. 1, which comprises a main tube 1; the inner cavity of the main pipe 1 is sequentially provided with a cracking area 2, a washing area 4 and an elution area 6 from top to bottom; hydrophobic sealing layers are respectively arranged between the cracking zone 2 and the washing zone 4 and between the washing zone 4 and the elution zone 6 for separation, and magnetic bead washing liquid and nucleic acid eluent are respectively arranged in the washing zone 4 and the elution zone 6; the inner cavity of the main pipe 1 is also provided with a magnetic track 7 extending from the cracking zone 2 to the elution zone 6, one end of the magnetic track 7 is closed, and the other end of the magnetic track is provided with an opening for the magnetic rod to extend into. The bottom end of the magnetic track 7 is integrally formed with the main pipe 1 and the bottom end of the magnetic track 7 closes the top end opening. In this way, the hydrophobic sealing layer separates the cell lysis solution in the lysis zone 2 from the magnetic bead washing solution in the washing zone 4, and separates the magnetic bead washing solution in the washing zone 4 from the nucleic acid eluent in the elution zone 6; after a magnetic rod is placed into an inner hole of the magnetic track 7 from the top end opening of the magnetic track 7, magnetic beads in the cracking zone 2 are sucked and attached to the outer wall of the magnetic track 7 by using the magnetic force of the magnetic rod; the magnetic rod moves downwards to drive the magnetic beads to move downwards to penetrate through the hydrophobic sealing layer to enter the washing area 4 and the elution area 6 respectively for washing and elution, so that the extraction of nucleic acid is completed; in this way, the magnetic beads are attached to the outer wall of the magnetic track 7 and move along with the magnetic rod, the outer wall of the whole magnetic track 7 can be used for moving the magnetic beads without being limited by the number of magnetic bead channels, a large number of magnetic beads can be driven at one time, and the nucleic acid extraction efficiency is improved; meanwhile, the magnetic beads are uniformly distributed on the outer wall of the magnetic track with larger area and are not stacked, so that the magnetic beads are more stable in movement.

Additionally, the utility model also discloses an integration nucleic acid carries test tube soon, include integration nucleic acid carry test tube and bar magnet soon, the bar magnet outer wall cooperatees with 7 holes in the magnetic track.

The inner cavity of the main pipe 1 is provided with a first separating plug 3 and a second separating plug 5; annular gaps are formed between the outer wall of the magnetic track 7 and the first separating plug 3 and the second separating plug 5, so that the magnetic beads can be attached to the outer wall of the magnetic track 7 and move downwards through the annular gaps; the hydrophobic sealing layers are respectively positioned in the first separating plug 3 and the second separating plug 5 and fill and seal the inner cavity of the main pipe 1 at the positions so as to be beneficial to positioning the solid hydrophobic sealing layers.

The hydrophobic sealing layer is a paraffin or liquid-phase hydrophobic layer; the first and second partition plugs 3 and 5 are both of an elastic material. The first separating plug 3 comprises a first plug body 31, a first lower conical surface 32 in an inclined direction, a first upper conical surface 33 in an inclined direction and a first supporting surface 34 in a horizontal direction; the inner cavity of the main pipe 1 is also provided with a first lower inclined surface 11 in an inclined direction, a first upper inclined surface 12 in an inclined direction and a first step surface 13 in a horizontal direction; the first lower tapered surface 32 and the first upper tapered surface 33 respectively abut against the first lower inclined surface 11 and the first upper inclined surface 12, and the first support surface 34 abuts against the first step surface 13. The inner cavity of the main pipe 1 is further provided with a first protrusion 14, the first protrusion 14 protrudes out of the inner surface of the inner cavity of the cracking zone 2, and the first protrusion 14 is located above the upper surface of the first separating plug 3. The first step surface 13 and the first protrusion 14 are respectively contacted with the first supporting surface 34 and the upper surface of the first plug body 31 so as to limit the axial direction of the first separating plug 3; the first lower inclined surface 11 and the first upper inclined surface 12 in the inclined direction are respectively contacted with the first lower conical surface 32 and the first upper conical surface 33 so as to limit the radial direction of the first separating plug 3; so that the first separating plug 3 can be clamped on the inner wall of the main pipe 1 by using the elasticity of the first separating plug and the limit of the first separating plug and the inner wall of the main pipe 1.

The second separating plug 5 comprises a second plug body 51, a second supporting surface 52 positioned in the horizontal direction of the bottom and a second taper surface 53 in the inclined direction; the inner cavity of the main pipe 1 is also provided with a second step surface 15 in the horizontal direction and a second inclined surface 16 in the inclined direction; the second support surface 52 abuts the second step surface 15, and the second tapered surface 53 abuts the second inclined surface 16. The inner cavity of the main pipe 1 is further provided with a second protrusion 17, the second protrusion 17 protrudes from the inner surface of the inner cavity of the washing zone 4, and the second protrusion 17 is located above the upper surface of the second separating plug 5. The second step surface 15 and the second bulge 17 are respectively contacted with the second supporting surface 52 and the upper surface of the second plug body 51 so as to limit the axial direction of the second separating plug 5; the second inclined surface 16 in the inclined direction is in contact with the second conical surface 53 so as to limit the radial direction of the second separating plug 5; so that the second separating plug 5 can be clamped on the inner wall of the main pipe 1 by using the elasticity of the second separating plug and the limit of the second separating plug and the inner wall of the main pipe 1.

A plurality of clapboards are arranged in the elution area 6, and the radial two ends of the clapboards are respectively connected with the outer wall of the magnetic track 7 and the inner wall of the cavity of the main pipe 1 so as to divide the elution area 6 into a plurality of branch pipes. The elution liquid is divided into a plurality of mutually independent parts by the branch pipes, so that the control analysis can be simultaneously carried out on the plurality of parts of elution liquid, the influence of accidental factors is eliminated, and the accuracy of nucleic acid detection is improved.

The top end of the main pipe 1 is provided with a pipe cover 18, and the pipe cover 18 is screwed with the external thread of the outer wall of the main pipe 1 through the internal thread to prevent external pollutants from entering the main pipe 1; the tube cover 18 is provided with a positioning hole 181 which vertically penetrates through, and the positioning hole 181 is matched with the outer wall of the top opening of the magnetic track 7 so as to further limit the upper part of the magnetic track 7 and prevent the upper part from deviating.

The bottom of the elution zone 6 is provided with a base 61; the upper end of the base 61 is provided with a matching hole 62 corresponding to the shape of the outer wall of the elution area 6, and the matching hole 62 is embedded with the outer wall of the elution area 6 so as to enable the main pipe 1 to be connected with or separated from the base 61; the lower end of the base 61 is provided with a fixing hole 63, and the fixing hole 63 is embedded with a corresponding bulge of the main pipe fixing equipment. Thereby make the corresponding arch of being responsible for fixed equipment can be through driving the motion of base 61 and then drive and be responsible for 1 and move to different stations, conveniently be responsible for 1 and link up with other processing equipment mutually, be favorable to other processing equipment to be responsible for 1 and carry out automation and move operations such as.

The elution area 6 is detachably connected with the main pipe 1; the top height of the base 61 is aligned with the top height of the elution area 6, the outer wall of the base 61 is provided with threads, and the threads on the outer wall of the base 61 are screwed with the threaded cover. When the solution processed by the main pipe 1 needs to be taken out, the elution area 6 is detached from the main pipe 1, and then the threaded cover is screwed with the outer wall of the base 61, so that the opening of the elution area 6 is closed, and the elution area 6 is separated from the main pipe 1.

When in use: 1) firstly, a lysis solution and magnetic beads are preset in the lysis zone 2, sample cells to be detected are added into the lysis zone 2 after the tube cover 18 is unscrewed, and the sample cells are lysed by the lysis solution and release complete nucleic acids and are combined with the magnetic beads; or directly adding the solution containing the sample cells to be detected and the magnetic beads after the lysis to the lysis zone 2; 2) inserting a magnetic rod from the upper end opening of the magnetic track 7 and moving down to the washing area 4, wherein the magnetic rod attracts the magnetic beads to be attached to the outer wall of the magnetic track 7 and moves down to the washing area 4 through a hydrophobic sealing layer in the first separation plug 3 along with the magnetic rod; when the hydrophobic sealing layer is paraffin, the main pipe 1 is heated to melt the paraffin; 3) after the magnetic bead washing liquid removes some impurities such as protein and inorganic salt in the nucleic acid combined with the magnetic beads, the magnetic rod drives the magnetic beads to continuously move downwards to pass through the hydrophobic sealing layer in the second separating plug 5 to reach the elution area 6; 4) contacting the nucleic acid eluent with the washed magnetic beads to elute the nucleic acids combined on the magnetic beads into the nucleic acid eluent in the elution area 6; 5) carrying out PCR or isothermal amplification reaction on nucleic acid in nucleic acid eluent, introducing a molecular beacon into the nucleic acid eluent, enabling external equipment to observe the PCR or isothermal amplification reaction in real time through fluorescent quantitative detection, and obtaining the quantity of sample nucleic acid by analyzing a fluorescence value; or the elution area 6 is detached from the main pipe 1, the outer wall thread of the base 61 is screwed with the thread cover to close the opening of the elution area 6, and then the elution area is taken to other equipment for nucleic acid detection to complete the integrated nucleic acid quick extraction process.

Example 2

An integrated nucleic acid rapid-extraction tube as shown in FIG. 4, which comprises a main tube 1; the inner cavity of the main pipe 1 is sequentially provided with a cracking area 2, a washing area 4 and an elution area 6 from top to bottom; hydrophobic sealing layers are respectively arranged between the cracking zone 2 and the washing zone 4 and between the washing zone 4 and the elution zone 6 for separation, and magnetic bead washing liquid and nucleic acid eluent are respectively arranged in the washing zone 4 and the elution zone 6; the inner cavity of the main pipe 1 is also provided with a magnetic track 7 extending from the cracking zone 2 to the elution zone 6, one end of the magnetic track 7 is closed, and the other end of the magnetic track is provided with an opening for the magnetic rod to extend into. The bottom end of the magnetic track 7 is integrally formed with the main pipe 1 and the bottom end of the magnetic track 7 is open and closed. In this way, the hydrophobic sealing layer separates the cell lysis solution in the lysis zone 2 from the magnetic bead washing solution in the washing zone 4, and separates the magnetic bead washing solution in the washing zone 4 from the nucleic acid eluent in the elution zone 6; after the magnetic rod is placed into an inner hole of the magnetic track 7 from the bottom opening of the magnetic track 7, the magnetic beads in the cracking zone 2 are absorbed and attached to the outer wall of the magnetic track 7 by using the magnetic force of the magnetic rod; the magnetic rod moves downwards to drive the magnetic beads to move downwards to penetrate through the hydrophobic sealing layer to enter the washing area 4 and the elution area 6 respectively for washing and elution, so that the extraction of nucleic acid is completed; in this way, the magnetic beads are attached to the outer wall of the magnetic track 7 and move along with the magnetic rod, the outer wall of the whole magnetic track 7 can be used for moving the magnetic beads without being limited by the number of magnetic bead channels, a large number of magnetic beads can be driven at one time, and the nucleic acid extraction efficiency is improved; meanwhile, the magnetic beads are uniformly distributed on the outer wall of the magnetic track with larger area and are not stacked, so that the magnetic beads are more stable in movement.

The top end of the main pipe 1 is provided with a pipe cover 180, and the pipe cover 180 comprises a cover body 182, a storage tank 183 and a sealing cover 184; the sealing cover 184 is positioned at the top end of the storage groove 183 and is opened and closed; the bottom of the storing groove 183 is provided with a blanking hole 185, the storing groove 183 is communicated with the cracking zone 2 of the main pipe 1 through the blanking hole 185, and the tin foil is arranged in the blanking hole 185 to seal the same. The sealing cover 184 is made of elastic material, and the top end opening of the storage groove 183 is sealed by the interference fit of the sealing cover 184 and the storage groove 183. When the magnetic beads or the proteinase k or the mixture of the magnetic beads and the proteinase k are preset in the storage tank 183 and need to be added to the lysis zone 2 of the main pipe 1, the sealing cover 184 is opened and the tinfoil at the position of the blanking hole 185 is broken, so that the magnetic beads or the proteinase k or the mixture of the magnetic beads and the proteinase k fall into the lysis zone 2 along the blanking hole 185, and the addition of the magnetic beads or the proteinase k is completed. In this way, the magnetic beads or proteinase k can be preset in the storage tank 183 and can be added by directly breaking the tinfoil, so that the operator does not need to suck and move the magnetic beads or proteinase k again from other positions, and the operation steps are reduced. Meanwhile, the magnetic beads or proteinase k preset in the storage tank 183 can be transported and stored simultaneously with the main pipe 1 without being transported and stored separately, so that the transportation and storage processes are simplified, and the integrated extraction of nucleic acid is facilitated.

A base 60 is arranged at the bottom of the elution area 6, and a liquid outlet at the bottom of the elution area 6 is open and is communicated with the outside; the base 60 is provided with a collecting hole 64 and a magnetic track connecting hole 65, the collecting hole 64 is communicated with a liquid outlet at the bottom of the elution zone 6, and the upper end of the collecting hole is embedded with the outer wall of the liquid outlet at the bottom of the elution zone 6; the lower end opening of the magnetic track is communicated with the outside through a magnetic track connecting hole 65 and the magnetic track connecting hole 65 is matched with the outer wall of the magnetic rod. After the nucleic acid eluent in the elution area 6 contacts the washed magnetic beads to elute the nucleic acid combined on the magnetic beads to the nucleic acid eluent in the elution area 6, the external device detects the sample nucleic acid through optical detection or pulls the base 60 from the elution area 6 of the main pipe 1, and then collects the solution in the base 60 and takes the solution to other devices to perform nucleic acid detection, thereby completing the integrated nucleic acid fast-extraction process. A plurality of ribs 21 are vertically and fixedly arranged on the inner wall of the cracking zone 2, and the plurality of ribs 21 protrude out of the inner wall of the cracking zone 2, so that the plurality of ribs 21 can help uniformly mix when the solution in the cracking zone 2 is vibrated; the outer wall of the base 60 is provided with threads, and the threads on the outer wall of the base 60 are screwed with the threaded cover so as to close the opening of the disassembled elution area 6.

When in use: 1) presetting lysis solution in the lysis zone 2, presetting magnetic beads or proteinase k or a mixture of the magnetic beads and the proteinase k into a storage tank 183, opening a sealing cover 184, breaking a tinfoil at the position of a blanking hole 185, and adding sample cells to be detected into the lysis zone 2; the magnetic beads or the proteinase k or the mixture of the magnetic beads and the proteinase k also fall into the lysis zone 2 along the blanking hole 185, and the sample cells are lysed by the lysis solution to release complete nucleic acids and are combined with the magnetic beads; or the tinfoil at the 185 position of the blanking hole is directly broken and the solution containing the sample cells and magnetic beads to be detected after the lysis is added into the lysis zone 2 for the lysis; 2) inserting a magnetic rod into the cracking zone 2 from the opening at the lower end of the magnetic track 7, moving the magnetic rod downwards to the washing zone 4, attracting magnetic beads to be attached to the outer wall of the magnetic track 7 and moving the magnetic rod downwards to the washing zone 4 after passing through a hydrophobic sealing layer; when the hydrophobic sealing layer is paraffin, the main pipe 1 is heated to melt the paraffin; 3) after the magnetic bead washing liquid removes some impurities such as protein and inorganic salt in the nucleic acid combined with the magnetic beads, the magnetic rod drives the magnetic beads to continuously move downwards to pass through the hydrophobic sealing layer in the second separating plug 5 to reach the elution area 6; 4) contacting the nucleic acid eluent with the washed magnetic beads to elute the nucleic acids combined on the magnetic beads into the nucleic acid eluent in the elution area 6; 5) carrying out PCR or isothermal amplification reaction on nucleic acid in nucleic acid eluent, introducing a molecular beacon into the nucleic acid eluent, enabling external equipment to observe the PCR or isothermal amplification reaction in real time through fluorescent quantitative detection, and obtaining the quantity of sample nucleic acid by analyzing a fluorescence value; or after the base 60 is pulled out from the elution area 6 of the main pipe 1, the thread on the outer wall of the base 60 is screwed with the thread cover so as to close the opening of the elution area 6 and carry the nucleic acid to other equipment for nucleic acid detection, thereby completing the integrated nucleic acid rapid extraction process.

Claims

1. An integrated nucleic acid rapid-extraction test tube, which comprises a main tube (1); the inner cavity of the main pipe (1) is sequentially provided with a cracking area (2), a washing area (4) and an elution area (6) from top to bottom; hydrophobic sealing layers are respectively arranged between the lysis zone (2) and the washing zone (4) and between the washing zone (4) and the elution zone (6) for separating, and magnetic bead washing liquid and nucleic acid eluent are respectively arranged in the washing zone (4) and the elution zone (6); the device is characterized in that the inner cavity of the main pipe (1) is also provided with a magnetic track (7) extending from the cracking zone (2) to the elution zone (6), one end of the magnetic track (7) is closed, and the other end of the magnetic track is provided with an opening for the magnetic rod to extend into.

2. The integrated nucleic acid rapid-extraction tube according to claim 1, wherein the bottom end of the magnetic track (7) is integrally formed with the main tube (1) and the bottom end of the magnetic track (7) closes the top end opening, or the bottom end of the magnetic track (7) is integrally formed with the main tube (1) and the bottom end of the magnetic track (7) opens the top end closing.

3. The integrated nucleic acid rapid-extraction test tube according to claim 1, wherein the inner cavity of the main tube (1) is provided with a first separating plug (3) and a second separating plug (5); annular gaps are formed between the outer wall of the magnetic track (7) and the first separating plug (3) and the second separating plug (5); the hydrophobic sealing layer is respectively positioned in the first separating plug (3) and the second separating plug (5) and fills and seals the inner cavity of the main pipe (1) at the position.

4. The integrated nucleic acid rapid-extraction test tube according to claim 3, wherein the hydrophobic sealing layer is paraffin or a liquid-phase hydrophobic layer; the first separating plug (3) and the second separating plug (5) are made of elastic materials.

5. The integrated nucleic acid rapid-extraction tube according to claim 3, wherein the first separating plug (3) comprises a first plug body (31), a first lower tapered surface (32) in an inclined direction, a first upper tapered surface (33) in an inclined direction, and a first supporting surface (34) in a horizontal direction; the inner cavity of the main pipe (1) is also provided with a first lower inclined surface (11) in the inclined direction, a first upper inclined surface (12) in the inclined direction and a first step surface (13) in the horizontal direction; the first lower conical surface (32) and the first upper conical surface (33) are respectively attached to the first lower inclined surface (11) and the first upper inclined surface (12), and the first supporting surface (34) is attached to the first step surface (13).

6. The integrated nucleic acid rapid-lift tube according to claim 3, wherein the inner cavity of the main tube (1) is further provided with a first protrusion (14), the first protrusion (14) protrudes from the inner surface of the inner cavity of the lysis zone (2), and the first protrusion (14) is located above the upper surface of the first separating plug (3).

7. The integrated nucleic acid quick-extraction tube according to claim 3, wherein the second partition plug (5) comprises a second plug body (51), a second support surface (52) in the horizontal direction of the bottom and a second taper surface (53) in the inclined direction; the inner cavity of the main pipe (1) is also provided with a second step surface (15) in the horizontal direction and a second inclined surface (16) in the inclined direction; the second support surface (52) is attached to the second step surface (15), and the second taper surface (53) is attached to the second inclined surface (16).

8. The integrated nucleic acid rapid-extraction tube according to claim 3, wherein the inner cavity of the main tube (1) is further provided with a second protrusion (17), the second protrusion (17) protrudes from the inner surface of the inner cavity of the washing zone (4), and the second protrusion (17) is located above the upper surface of the second partition plug (5).

9. The integrated nucleic acid rapid-extraction test tube according to claim 1, wherein a plurality of partition plates are arranged in the elution area (6), and the plurality of partition plates are respectively connected with the outer wall of the magnetic track (7) and the inner wall of the cavity of the main tube (1) at two radial ends so as to divide the elution area (6) into a plurality of branch tubes.

10. The integrated nucleic acid rapid-extraction test tube according to claim 1, wherein the top end of the main tube (1) is provided with a first tube cover (18), and the first tube cover (18) is screwed with the external thread of the outer wall of the main tube (1) through internal threads; the first pipe cover (18) is provided with a positioning hole (181) which vertically penetrates through, and the positioning hole (181) is matched with the outer wall of the top opening of the magnetic track (7).

11. The integrated nucleic acid rapid-extraction test tube according to claim 1, wherein the bottom of the elution zone (6) is provided with a second base (61); the upper end of the second base (61) is provided with a matching hole (62) corresponding to the shape of the outer wall of the elution area (6), and the matching hole (62) is embedded with the outer wall of the elution area (6); the lower end of the second base (61) is provided with a fixing hole (63), and the fixing hole (63) is embedded with a corresponding bulge of the main pipe fixing equipment.

12. The integrated nucleic acid rapid-extraction tube according to claim 11, wherein the elution zone (6) is detachably connected with the main tube (1); the top end of the second base (61) is aligned with the top end of the elution area (6), the outer wall of the second base (61) is provided with threads, and the threads on the outer wall of the second base (61) are screwed with the threaded cover.

13. The integrated nucleic acid rapid-extraction test tube according to claim 1, wherein a first tube cover (180) is arranged at the top end of the main tube (1), and the first tube cover (180) comprises a cover body (182), a storage tank (183) and a sealing cover (184); the sealing cover (184) is positioned at the top end of the storage groove (183) and is closed; the bottom of the storage tank (183) is provided with a blanking hole (185), the storage tank (183) is communicated with the cracking area (2) of the main pipe (1) through the blanking hole (185), and the blanking hole (185) is internally provided with tinfoil to seal the blanking hole.

14. The integrated nucleic acid rapid-extraction test tube according to claim 13, wherein the sealing cover (184) is made of elastic material, and the sealing cover (184) is in interference fit with the storage groove (183) to close the top end opening of the storage groove (183).

15. The integrated nucleic acid rapid-extraction test tube according to claim 1, wherein a first base (60) is arranged at the bottom of the elution area (6), and a liquid outlet at the bottom of the elution area (6) is open and is communicated with the outside; the first base (60) is provided with a collecting hole (64) and a magnetic track connecting hole (65), the collecting hole (64) is communicated with a liquid outlet at the bottom of the elution area (6), and the upper end of the collecting hole is embedded with the outer wall of the liquid outlet at the bottom of the elution area (6); the lower end opening of the magnetic track is communicated with the outside through a magnetic track connecting hole (65), and the magnetic track connecting hole (65) is matched with the outer wall of the magnetic rod.

16. The integrated nucleic acid rapid-extraction test tube according to claim 15, wherein a plurality of ribs (21) are vertically and fixedly arranged on the inner wall of the lysis zone (2), and the plurality of ribs (21) protrude out of the inner wall of the lysis zone (2).

17. The integrated nucleic acid rapid-extraction test tube according to claim 15, wherein the outer wall of the first base (60) is provided with threads, and the threads on the outer wall of the first base (60) are screwed with the threaded cap.

18. An integrated nucleic acid rapid-extraction and detection device, characterized in that the device comprises an integrated nucleic acid rapid-extraction test tube and a magnetic rod according to any one of claims 1 to 17, wherein the outer wall of the magnetic rod is matched with the inner hole of a magnetic track (7).