CN109666580A - A kind of peripheral blood mononuclear cells automation extraction element and its extracting method - Google Patents
A kind of peripheral blood mononuclear cells automation extraction element and its extracting method Download PDFInfo
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- CN109666580A CN109666580A CN201811608553.XA CN201811608553A CN109666580A CN 109666580 A CN109666580 A CN 109666580A CN 201811608553 A CN201811608553 A CN 201811608553A CN 109666580 A CN109666580 A CN 109666580A
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Abstract
The invention discloses a kind of peripheral blood mononuclear cells automation extraction element and its extracting methods, its extraction element includes pedestal, stabilizer blade is fixedly installed on the bottom four corners position of the pedestal, and mounting groove is offered at the top of pedestal, it is fixedly installed with speed regulating motor and fixing seat respectively in the bottom interior wall of the mounting groove, the speed regulating motor is located at the side of fixing seat, and the top side of fixing seat is equidistantly rotatably connected to multiple second synchronizing wheels, is fixedly installed with the first synchronizing wheel on the output shaft of the speed regulating motor.Operation of the present invention is simple, by the way that centrifuge tube to be filled to peripheral blood mononuclear cells ontology and reagent respectively, starting driving motor drives multiple centrifuge tubes to be rotated later, it is centrifuged, the peripheral blood mononuclear cells ontology after the completion of centrifugation can be placed in different containers later, so as to replace manual operations, and batch can be carried out while being operated, there is certain simplicity.
Description
Technical field
The present invention relates to the field of medical instrument technology more particularly to a kind of peripheral blood mononuclear cells to automate extraction element
And its extracting method.
Background technique
Blood of human body is known as whole blood after anticoagulation, is broadly divided into three layers after whole blood centrifugation, is followed successively by from top to bottom
Plasma layer, tunica albuginea layer and red blood cell layer.Plasma layer mainly includes blood plasma, water, protein, salt and various ions etc.；Tunica albuginea layer
It mainly include blood platelet, lymphocyte, peripheral blood mononuclear cells and granulocyte, these tangible cell specific gravity are close, therefore,
It is gathered in tunica albuginea layer；Red blood cell layer can simply be divided into Young erythrocyte and normocyte, since the two is in red blood cell not
Same growth period, specific gravity slightly have difference.
Wherein, peripheral blood mononuclear cells detects lymphocyte, the immunization therapy of cancer individuation, stem cell in vitro at present
There is important application in treatment and relevant research, therefore, it will usually it is extracted and is detected, however current
The extraction of nucleus is all to be operated by manpower, and cannot extract in batches, so can have certain inconvenience, so I
Propose a kind of peripheral blood mononuclear cells automation extraction element and its extracting method, for solving above-mentioned proposed ask
Topic.
Summary of the invention
Technical problems based on background technology, the invention proposes a kind of automations of peripheral blood mononuclear cells to extract
Device and its extracting method.
A kind of peripheral blood mononuclear cells proposed by the present invention automates extraction element, including pedestal, the bottom of the pedestal
It is fixedly installed with stabilizer blade on portion's corner location, and offers mounting groove at the top of pedestal, in the bottom interior wall of the mounting groove
It is fixedly installed with speed regulating motor and fixing seat respectively, the speed regulating motor is located at the side of fixing seat, and the top side of fixing seat
Multiple second synchronizing wheels are equidistantly rotatably connected to, the first synchronizing wheel, institute are fixedly installed on the output shaft of the speed regulating motor
State and be rotatably connected to the same synchronous belt in the first synchronizing wheel and multiple second synchronizing wheels, be installed in second synchronizing wheel from
Heart pipe, and be symmetrically fixedly mounted on the side inner wall of mounting groove there are two limited post, lifting is slidably connected on the limited post
Column, and the top of pedestal is all extended at the top of two lifting columns, two lifting columns lateral roof close to each other is fixedly connected
There is the same mounting plate, multiple connecting tubes is fixedly connected on the mounting plate, and the top of connecting tube extends to mounting plate
Top, the bottom end of the connecting tube extend in centrifuge tube and are fixedly connected with restraining position ring block, and the inner wall of stop collar and centrifuge tube
In sealing contact, being sealedly and fixedly connected in the bottom interior wall of the centrifuge tube, there are three outlet tubes, and the bottom end of three outlet tubes point
Not Guan Chuan fixing seat and mounting groove bottom interior wall and extend to the lower section of pedestal, the top slide of the mounting plate is connected with branch
Fagging, and multiple placement racks are fixedly installed in support plate, the bottom of the placement rack is fixedly installed with communicating pipe, the connection
The bottom end of pipe extends in connecting tube and is slidably connected with the sealing of the inner wall of connecting tube.
Preferably, sealing is fixedly installed with fixed plate in the centrifuge tube, and three outlet tubes are fixedly mounted on fixation
On plate, it is fixedly connected with solenoid valve in the outlet tube, density biography is fixedly installed in the side bottom interior wall of the centrifuge tube
Sensor, and density sensor is electrically connected with three solenoid valves respectively.
Preferably, the limited post is equipped with mobile chamber, slidably connects first movement respectively in the mobile cavity room
Plate and the second movable plate, and the second movable plate is located at the top of first movement plate, the first movement plate and the second movable plate phase
Contact, the bottom end of the lifting column extend in mobile cavity room and be fixedly connected at the top of the second movable plate, the mobile cavity
Compressed spring is fixedly connected in the bottom interior wall of room, and the top of compressed spring is fixedly connected with the bottom of first movement plate.
Preferably, the top of the pedestal is symmetrically fixedly mounted there are two driving motor, and on the output shaft of driving motor
It is fixedly installed with semi-circle gear, the side of the lifting column is fixedly installed with rack gear, and rack gear is meshed with semi-circle gear.
Preferably, fixing sleeve is equipped with cleaning cotton on the stop collar, and the inner wall of cleaning cotton and centrifuge tube is in close contact.
Preferably, placing groove is offered at the top of the placement rack, and connection is offered in the bottom interior wall of the placing groove
Hole, and connecting hole is connected with communicating pipe, and pipe close, the pipe close and connecting tube movable sealing are fixedly installed in the connecting tube
It clamps.
Preferably, sealing slidably connects push plate in the placing groove, and push rod is fixedly installed at the top of the push plate, more
The top of a push rod all extends to the top of placement rack and is fixedly installed with the same connecting rod.
Preferably, the bottom of the connecting rod is fixedly connected with drawstring, and rotation is fixedly installed at the top of the support plate
The bottom end fixation of motor, the drawstring is wound around on the output shaft of rotary electric machine.
Preferably, the bottom symmetrical of the connecting rod is fixedly mounted there are two support column, symmetrically opens up in the support plate
There are two slide opening, the bottom end of the support column is slidably connected through slide opening and with the inner wall of slide opening, is arranged on the support column
Support spring, the top of the support spring are fixedly connected with the bottom of connecting rod, the bottom end of the support spring and support plate
Top be fixedly connected.
The invention also provides a kind of peripheral blood mononuclear cells to automate extracting method, comprising the following steps:
Multiple centrifuge tubes: being installed in the second synchronizing wheel by S1 correspondingly first, and outlet tube can be through installation at this time
The bottom interior wall of slot；
S2: starting driving motor drives semi-circle gear to be rotated, and when semi-circle gear is rotated, can move down
Mounting plate drives connecting tube to move down, until stop collar is moved in centrifuge tube；
S3: drawstring can be wound around on the output shaft of rotary electric machine by starting rotary electric machine, can drive connection at this time
Bar moves down, and when connecting rod moves down, push plate can be driven to move down by push rod, at this time can be by peripheral blood list
A nucleus bulk liquid pushes in centrifuge tube；
S4: starting speed regulating motor drives the first synchronizing wheel to be rotated, and when the first synchronizing wheel is rotated, can pass through
Synchronous belt drives multiple second synchronizing wheels to be rotated, and multiple centrifuge tubes will rotate in same direction simultaneously at this time, and human peripheral blood is single
Nucleus ontology is centrifuged；
S5: after centrifugation, stratification state can be presented in peripheral blood mononuclear cells ontology, later according to density sensor
Three solenoid valves can be successively respectively started in detection, at this time can be by the peripheral blood mononuclear cells bulk liquid after layering point
It is not placed in different containers.
The beneficial effects of the present invention are:
1, multiple centrifuge tubes are installed on correspondingly in the second synchronizing wheel first, outlet tube can run through mounting groove at this time
Bottom interior wall, later start driving motor drive semi-circle gear rotated, can be downward when semi-circle gear is rotated
Mobile mounting plate drives connecting tube to move down, until stop collar is moved in centrifuge tube, starting rotary electric machine later can be with
Drawstring is wound around on the output shaft of rotary electric machine, connecting rod can be driven to move down at this time, is moved down in connecting rod
When, push plate can be driven to move down by push rod, peripheral blood mononuclear cells bulk liquid can be pushed to centrifugation at this time
In pipe, after filling, starting speed regulating motor drives the first synchronizing wheel to be rotated, can when the first synchronizing wheel is rotated
To drive multiple second synchronizing wheels to be rotated by synchronous belt, multiple centrifuge tubes will rotate in same direction simultaneously at this time, to periphery
Blood mononuclear cell ontology is centrifuged, and after centrifugation, stratification state, Zhi Hougen can be presented in peripheral blood mononuclear cells ontology
Three solenoid valves can successively be respectively started according to the detection of density sensor, it at this time can be by the single core of peripheral blood after layering
Cellular entities liquid is individually placed in different containers.
2, operation of the present invention is simple, by the way that centrifuge tube to be filled to peripheral blood mononuclear cells ontology and reagent respectively, later
Starting driving motor drives multiple centrifuge tubes to be rotated, and is centrifuged, later can be by the single core of peripheral blood after the completion of centrifugation
Cellular entities are placed in different containers, so as to replace manual operations, and can be carried out batch while be operated, have one
Fixed simplicity.
Detailed description of the invention
Fig. 1 is the structural front view that a kind of peripheral blood mononuclear cells proposed by the present invention automates extraction element；
Fig. 2 is the limit rod structure main view that a kind of peripheral blood mononuclear cells proposed by the present invention automates extraction element
Figure；
Fig. 3 is the A schematic enlarged-scale view that a kind of peripheral blood mononuclear cells proposed by the present invention automates extraction element；
Fig. 4 is the B structure enlarged drawing that a kind of peripheral blood mononuclear cells proposed by the present invention automates extraction element；
Fig. 5 is the C-structure enlarged drawing that a kind of peripheral blood mononuclear cells proposed by the present invention automates extraction element.
In figure: 1 pedestal, 2 mounting grooves, 3 speed regulating motors, 4 first synchronizing wheels, 5 second synchronizing wheels, 6 synchronous belts, 7 centrifuge tubes,
8 limited posts, 9 lifting columns, 10 driving motors, 11 semi-circle gears, 12 mounting plates, 13 connecting tubes, 14 stop collars, 15 support plates, 16
Placement rack, 17 connecting rods, 18 rotary electric machines, 19 drawstrings, 20 support columns, 21 compressed springs, 22 fixed plates, 23 outlet tubes, 24 electricity
Magnet valve, 25 density sensors, 26 placing grooves, 27 push plates, 28 push rods, 29 communicating pipes, 30 pipe closes, 31 mobile chambers, 32 first are moved
Movable plate, 33 second movable plates, 34 support springs.
Specific embodiment
Combined with specific embodiments below the present invention is made further to explain.
Embodiment
With reference to Fig. 1-5, a kind of peripheral blood mononuclear cells automation extraction element, including pedestal are proposed in the present embodiment
1, stabilizer blade is fixedly installed on the bottom four corners position of pedestal 1, and the top of pedestal 1 offers mounting groove 2, mounting groove 2
Speed regulating motor 3 and fixing seat are fixedly installed in bottom interior wall respectively, speed regulating motor 3 is located at the side of fixing seat, and fixing seat
Top side be equidistantly rotatably connected to multiple second synchronizing wheels 5, it is synchronous that first is fixedly installed on the output shaft of speed regulating motor 3
4 are taken turns, the same synchronous belt 6 is rotatably connected in the first synchronizing wheel 4 and multiple second synchronizing wheels 5, is installed in the second synchronizing wheel 5
Centrifuge tube 7, and be symmetrically fixedly mounted on the side inner wall of mounting groove 2 there are two limited post 8, liter is slidably connected on limited post 8
Column 9 drops, and the top of two lifting columns 9 all extends to the top of pedestal 1, two lifting columns 9 lateral roof close to each other is solid
Surely it is connected with the same mounting plate 12, multiple connecting tubes 13 are fixedly connected on mounting plate 12, and the top of connecting tube 13 extends
To the top of mounting plate 12, the bottom end of connecting tube 13 extends in centrifuge tube 7 and is fixedly connected with restraining position ring block 14, and stop collar 14
It is in sealing contact with the inner wall of centrifuge tube 7, it is sealedly and fixedly connected in the bottom interior wall of centrifuge tube 7 there are three outlet tube 23, and three
The bottom end of outlet tube 23 extends through the bottom interior wall of fixing seat and mounting groove 2 and extends to the lower section of pedestal 1, mounting plate 12
Top slide is connected with support plate 15, and multiple placement racks 16 are fixedly installed in support plate 15, and the bottom of placement rack 16 is fixed
Communicating pipe 29 is installed, the bottom end of communicating pipe 29 extends in connecting tube 13 and is slidably connected with the sealing of the inner wall of connecting tube 13,
Multiple centrifuge tubes 7 are installed in the second synchronizing wheel 5 correspondingly first, outlet tube 23 can run through the bottom of mounting groove 2 at this time
Portion's inner wall starts driving motor 10 later and semi-circle gear 11 is driven to be rotated, when semi-circle gear 11 is rotated, Ke Yixiang
Lower mobile mounting plate 12 drives connecting tube 13 to move down, until stop collar 14 is moved in centrifuge tube 7, starting turns later
Drawstring 19 can be wound around on the output shaft of rotary electric machine 18 by dynamic motor 18, can drive connecting rod 17 to moving down at this time
It is dynamic, when connecting rod 17 moves down, push plate 27 can be driven to move down by push rod 28, it at this time can be single by peripheral blood
Nucleus bulk liquid pushes in centrifuge tube 7, and after filling, starting speed regulating motor 3 drives 4 turns of the first synchronizing wheel
It is dynamic, when the first synchronizing wheel 4 is rotated, multiple second synchronizing wheels 5 can be driven to be rotated by synchronous belt 6, it is more at this time
A centrifuge tube 7 will rotate in same direction simultaneously, and Cytokines in Peripheral Blood Mononuclear ontology is centrifuged, after centrifugation, peripheral blood list
Stratification state can be presented in a nucleus ontology, and three electromagnetism can successively be respectively started according to the detection of density sensor 25 later
Peripheral blood mononuclear cells bulk liquid after layering can be individually placed in different containers by valve 24 at this time, the present invention
It is easy to operate, by the way that centrifuge tube 7 is filled peripheral blood mononuclear cells ontology and reagent respectively, starts driving motor later and drive
Multiple centrifuge tubes 7 are rotated, and are centrifuged, and later can be placed on the peripheral blood mononuclear cells ontology after the completion of centrifugation not
In same container, so as to replace manual operations, and batch can be carried out while being operated, there is certain simplicity, adjust the speed
The model of motor 3: GM12-N20VA, the model of driving motor 10 and rotary electric machine 18: R6493.
Peripheral blood mononuclear cells ontology can be divided into three layers after centrifugation: its upper layer is blood plasma and Hank's, and lower layer is
Red blood cell and granulocyte, middle layer are lymphocyte separation medium.
In the present embodiment, sealing is fixedly installed with fixed plate 22 in centrifuge tube 7, and three outlet tubes 23 are fixedly mounted on
In fixed plate 22, it is fixedly connected with solenoid valve 24 in outlet tube 23, is fixedly installed with density in the side bottom interior wall of centrifuge tube 7
Sensor 25, and density sensor 25 is electrically connected with three solenoid valves 24 respectively, limited post 8 is equipped with mobile chamber 31, moves
First movement plate 32 and the second movable plate 33 are slidably connected respectively in dynamic chamber 31, and the second movable plate 33 is located at first movement
The top of plate 32, first movement plate 32 are in contact with the second movable plate 33, and the bottom end of lifting column 9 extends in mobile chamber 31 simultaneously
It is fixedly connected with the top of the second movable plate 33, compressed spring 34 is fixedly connected in the bottom interior wall of mobile chamber 31, and press
The top of contracting spring 34 is fixedly connected with the bottom of first movement plate 32, the top of pedestal 1 be symmetrically fixedly mounted there are two driving
Motor 10, and semi-circle gear 11 is fixedly installed on the output shaft of driving motor 10, the side of lifting column 9 is fixedly mounted with teeth
Item, and rack gear is meshed with semi-circle gear 11, fixing sleeve is equipped with cleaning cotton on stop collar 14, and cleaning cotton and centrifuge tube 7 is interior
Wall is in close contact, and the top of placement rack 16 offers placing groove 26, connecting hole is offered in the bottom interior wall of placing groove 26, and even
It connecing hole to be connected with communicating pipe 29, pipe close 30 is fixedly installed in connecting tube 13, pipe close 30 and 13 movable sealing of connecting tube clamp,
Sealing slidably connects push plate 27 in placing groove 26, and the top of push plate 27 is fixedly installed with push rod 28, the top of multiple push rods 28
It all extends to the top of placement rack 16 and is fixedly installed with the same connecting rod 17, the bottom of connecting rod 17 is fixedly connected with drawstring
19, the top of support plate 15 is fixedly installed with rotary electric machine 18, the fixed output for being wound around rotary electric machine 18 in the bottom end of drawstring 19
On axis, the bottom symmetrical of connecting rod 17 is fixedly mounted there are two support column 20, symmetrically opens up that there are two slide opening, branch in support plate 15
The bottom end of dagger 20 is slidably connected through slide opening and with the inner wall of slide opening, and support spring 34 is arranged on support column 20, supports bullet
The top of spring 34 is fixedly connected with the bottom of connecting rod 17, and the bottom end of support spring 34 is fixedly connected with the top of support plate 15,
Multiple centrifuge tubes 7 are installed in the second synchronizing wheel 5 correspondingly first, outlet tube 23 can run through the bottom of mounting groove 2 at this time
Portion's inner wall starts driving motor 10 later and semi-circle gear 11 is driven to be rotated, when semi-circle gear 11 is rotated, Ke Yixiang
Lower mobile mounting plate 12 drives connecting tube 13 to move down, until stop collar 14 is moved in centrifuge tube 7, starting turns later
Drawstring 19 can be wound around on the output shaft of rotary electric machine 18 by dynamic motor 18, can drive connecting rod 17 to moving down at this time
It is dynamic, when connecting rod 17 moves down, push plate 27 can be driven to move down by push rod 28, it at this time can be single by peripheral blood
Nucleus bulk liquid pushes in centrifuge tube 7, and after filling, starting speed regulating motor 3 drives 4 turns of the first synchronizing wheel
It is dynamic, when the first synchronizing wheel 4 is rotated, multiple second synchronizing wheels 5 can be driven to be rotated by synchronous belt 6, it is more at this time
A centrifuge tube 7 will rotate in same direction simultaneously, and Cytokines in Peripheral Blood Mononuclear ontology is centrifuged, after centrifugation, peripheral blood list
Stratification state can be presented in a nucleus ontology, and three electromagnetism can successively be respectively started according to the detection of density sensor 25 later
Peripheral blood mononuclear cells bulk liquid after layering can be individually placed in different containers by valve 24 at this time, the present invention
It is easy to operate, by the way that centrifuge tube 7 is filled peripheral blood mononuclear cells ontology and reagent respectively, starts driving motor later and drive
Multiple centrifuge tubes 7 are rotated, and are centrifuged, and later can be placed on the peripheral blood mononuclear cells ontology after the completion of centrifugation not
In same container, so as to replace manual operations, and batch can be carried out while being operated, there is certain simplicity.
In the present embodiment, multiple centrifuge tubes 7 are installed in the second synchronizing wheel 5 correspondingly first, at this time outlet tube
23 can run through the bottom interior wall of mounting groove 2, start driving motor 10 later and semi-circle gear 11 is driven to be rotated, in semi-circle gear
11 when being rotated, and can move down mounting plate 12, and connecting tube 13 is driven to move down, until by stop collar 14 be moved to from
In heart pipe 7, drawstring 19 can be wound around on the output shaft of rotary electric machine 18 by starting rotary electric machine 18 later, at this time can band
Dynamic connecting rod 17 moves down, and when connecting rod 17 moves down, push plate 27 can be driven to move down by push rod 28, at this time
Peripheral blood mononuclear cells bulk liquid can be pushed in centrifuge tube 7, after filling, push down on support plate 15, make
It obtains communicating pipe 29 and 30 phase of pipe close clamps, centrifuge tube 7 can be made to be in sealing state at this time, start 3 band of speed regulating motor later
Dynamic first synchronizing wheel 4 is rotated, and when the first synchronizing wheel 4 is rotated, multiple second can be driven to synchronize by synchronous belt 6
Wheel 5 is rotated, and multiple centrifuge tubes 7 will rotate in same direction simultaneously at this time, and Cytokines in Peripheral Blood Mononuclear ontology is centrifuged,
After centrifugation, stratification state can be presented in peripheral blood mononuclear cells ontology, later can be according to according to the detection of density sensor 25
It is secondary that three solenoid valves 24 are respectively started, the peripheral blood mononuclear cells bulk liquid after layering can be individually placed to not at this time
In same container, after centrifugation, needs to clean centrifuge tube 7, launch clear water into placing groove 26 first, later successively
Start rotary electric machine 18, driving motor 10 and speed regulating motor 3, so that it may the clear water in placing groove 26 is pushed in centrifuge tube 7,
And centrifuge tube 7 can be made to be rotated, while stop collar 14 can be made to carry out longitudinally reciprocal movement, by cleaning cotton to from
The inner wall of heart pipe 7 is cleaned, and is flowed out later by three outlet tubes 23.
The invention also provides a kind of peripheral blood mononuclear cells to automate extracting method, comprising the following steps:
S1: multiple centrifuge tubes 7 are installed in the second synchronizing wheel 5 correspondingly first, outlet tube 23 can run through at this time
The bottom interior wall of mounting groove 2；
S2: starting driving motor 10 drives semi-circle gear 11 to be rotated, when semi-circle gear 11 is rotated, Ke Yixiang
Lower mobile mounting plate 12 drives connecting tube 13 to move down, until stop collar 14 is moved in centrifuge tube 7；
S3: drawstring 19 can be wound around on the output shaft of rotary electric machine 18 by starting rotary electric machine 18, at this time can band
Dynamic connecting rod 17 moves down, and when connecting rod 17 moves down, push plate 27 can be driven to move down by push rod 28, at this time
Peripheral blood mononuclear cells bulk liquid can be pushed in centrifuge tube 7；
S4: starting speed regulating motor 3 drives the first synchronizing wheel 4 to be rotated, can be with when the first synchronizing wheel 4 is rotated
Multiple second synchronizing wheels 5 are driven to be rotated by synchronous belt 6, multiple centrifuge tubes 7 will rotate in same direction simultaneously at this time, to periphery
Blood mononuclear cell ontology is centrifuged；
S5: after centrifugation, stratification state can be presented in peripheral blood mononuclear cells ontology, later according to density sensor 25
Detection three solenoid valves 24 can successively be respectively started, at this time can be by the peripheral blood mononuclear cells bulk liquid after layering
Body is individually placed in different containers.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (10)
1. a kind of peripheral blood mononuclear cells automates extraction element, including pedestal (1), which is characterized in that the pedestal (1)
It is fixedly installed with stabilizer blade on bottom four corners position, and offers mounting groove (2) at the top of pedestal (1), the mounting groove (2)
Speed regulating motor (3) and fixing seat are fixedly installed in bottom interior wall respectively, the speed regulating motor (3) is located at the side of fixing seat,
And the top side of fixing seat is equidistantly rotatably connected to multiple second synchronizing wheels (5), consolidates on the output shaft of the speed regulating motor (3)
Dingan County is equipped with the first synchronizing wheel (4), is rotatably connected on first synchronizing wheel (4) and multiple second synchronizing wheels (5) same
Synchronous belt (6) is installed with centrifuge tube (7) on second synchronizing wheel (5), and symmetrically fixes on the side inner wall of mounting groove (2)
There are two installations limited post (8), slidably connects lifting column (9) on the limited post (8), and the top of two lifting columns (9)
The top of pedestal (1) is all extended to, two lifting column (9) lateral roofs close to each other are fixedly connected with the same mounting plate
(12), it is fixedly connected with multiple connecting tubes (13) on the mounting plate (12), and the top of connecting tube (13) extends to mounting plate
(12) top, the bottom end of the connecting tube (13) extend in centrifuge tube (7) and are fixedly connected restraining position ring block (14), and limit
Ring (14) and the inner wall of centrifuge tube (7) are in sealing contact, and being sealedly and fixedly connected in the bottom interior wall of the centrifuge tube (7), there are three go out
Liquid pipe (23), and the bottom end of three outlet tubes (23) extends through the bottom interior wall of fixing seat and mounting groove (2) and extends to bottom
The lower section of seat (1), the top slide of the mounting plate (12) is connected with support plate (15), and is fixedly installed in support plate (15)
The bottom of multiple placement racks (16), the placement rack (16) is fixedly installed with communicating pipe (29), the bottom end of the communicating pipe (29)
It extends in connecting tube (13) and is slidably connected with the sealing of the inner wall of connecting tube (13).
2. a kind of peripheral blood mononuclear cells according to claim 1 automates extraction element, which is characterized in that it is described from
Sealing is fixedly installed with fixed plate (22) in heart pipe (7), and three outlet tubes (23) are fixedly mounted on fixed plate (22), institute
It states and is fixedly connected with solenoid valve (24) in outlet tube (23), is fixedly installed in the side bottom interior wall of the centrifuge tube (7) close
It spends sensor (25), and density sensor (25) is electrically connected with three solenoid valves (24) respectively.
3. a kind of peripheral blood mononuclear cells according to claim 1 automates extraction element, which is characterized in that the limit
Position column (8) is equipped with mobile chamber (31), slidably connects first movement plate (32) and the in the mobile chamber (31) respectively
Two movable plates (33), and the second movable plate (33) is located at the top of first movement plate (32), the first movement plate (32) and the
Two movable plates (33) are in contact, the bottom end of the lifting column (9) extend in mobile chamber (31) and with the second movable plate (33)
Top be fixedly connected, be fixedly connected with compressed spring (34) in the bottom interior wall of the mobile chamber (31), and compressed spring
(34) top is fixedly connected with the bottom of first movement plate (32).
4. a kind of peripheral blood mononuclear cells according to claim 1 automates extraction element, which is characterized in that the bottom
The top of seat (1) is symmetrically fixedly mounted there are two driving motor (10), and be fixedly installed on the output shaft of driving motor (10)
Semi-circle gear (11), the side of the lifting column (9) is fixedly installed with rack gear, and rack gear is meshed with semi-circle gear (11).
5. a kind of peripheral blood mononuclear cells according to claim 1 automates extraction element, which is characterized in that the limit
Fixing sleeve is equipped with cleaning cotton on position ring (14), and cleaning cotton and the inner wall of centrifuge tube (7) are in close contact.
6. a kind of peripheral blood mononuclear cells according to claim 5 automates extraction element, which is characterized in that described to put
It sets and offers placing groove (26) at the top of seat (16), connecting hole is offered in the bottom interior wall of the placing groove (26), and connect
Hole is connected with communicating pipe (29), is fixedly installed with pipe close (30) in the connecting tube (13), the pipe close (30) and connecting tube
(13) movable sealing clamps.
7. a kind of peripheral blood mononuclear cells according to claim 6 automates extraction element, which is characterized in that described to put
It sets sealing in slot (26) to slidably connect push plate (27), be fixedly installed with push rod (28) at the top of the push plate (27), it is multiple to push away
The top of bar (28) all extends to the top of placement rack (16) and is fixedly installed with the same connecting rod (17).
8. a kind of peripheral blood mononuclear cells according to claim 7 automates extraction element, which is characterized in that the company
The bottom of extension bar (17) is fixedly connected with drawstring (19), is fixedly installed with rotary electric machine (18) at the top of the support plate (15),
The bottom end fixation of the drawstring (19) is wound around on the output shaft of rotary electric machine (18).
9. a kind of peripheral blood mononuclear cells according to claim 7 automates extraction element, which is characterized in that the company
The bottom symmetrical of extension bar (17) is fixedly mounted there are two support column (20), symmetrically opens up that there are two sliding on the support plate (15)
The bottom end in hole, the support column (20) is slidably connected through slide opening and with the inner wall of slide opening, is arranged on the support column (20)
The top of support spring (34), the support spring (34) is fixedly connected with the bottom of connecting rod (17), the support spring
(34) it is fixedly connected at the top of bottom end and support plate (15).
10. a kind of peripheral blood mononuclear cells automates extracting method, which comprises the following steps:
S1: multiple centrifuge tubes (7) are installed on the second synchronizing wheel (5) correspondingly first, outlet tube (23) can pass through at this time
Wear the bottom interior wall of mounting groove (2)；
S2: starting driving motor (10) drives semi-circle gear (11) to be rotated, can be with when semi-circle gear (11) is rotated
It moves down mounting plate (12), connecting tube (13) is driven to move down, until stop collar (14) is moved in centrifuge tube (7)；
S3: drawstring (19) can be wound around on the output shaft of rotary electric machine (18) by starting rotary electric machine (18), at this time
Drive connecting rod (17) move down, when connecting rod (17) moves down, can by push rod (28) drive push plate (27) to
Lower movement at this time can push to peripheral blood mononuclear cells bulk liquid in centrifuge tube (7)；
S4: starting speed regulating motor (3) drives the first synchronizing wheel (4) to be rotated, can when the first synchronizing wheel (4) is rotated
To drive multiple second synchronizing wheels (5) to be rotated by synchronous belt (6), multiple centrifuge tubes (7) will turn in the same direction simultaneously at this time
Dynamic, Cytokines in Peripheral Blood Mononuclear ontology is centrifuged；
S5: after centrifugation, stratification state can be presented in peripheral blood mononuclear cells ontology, later according to density sensor (25)
Three solenoid valves (24) can be successively respectively started in detection, at this time can be by the peripheral blood mononuclear cells bulk liquid after layering
Body is individually placed in different containers.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110840156A (en) * | 2019-09-23 | 2020-02-28 | 赵树龙 | Intelligent mattress |
| CN113684122A (en) * | 2021-09-10 | 2021-11-23 | 江苏省肿瘤医院 | Equipment for rapidly separating and extracting peripheral blood mononuclear cells from blood sample |
| CN113680542A (en) * | 2021-08-25 | 2021-11-23 | 曹莹 | Adipose-derived stem cell collection device |
| CN114112567A (en) * | 2021-10-29 | 2022-03-01 | 郑州金域临床检验中心有限公司 | Preparation method of tuberculosis infection T cell spot test sample |
| CN115011557A (en) * | 2022-07-18 | 2022-09-06 | 泰州赛特生物医药科技有限公司 | Experimental identification device for non-blood-borne nucleated cells and application method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20010027156A1 (en) * | 1999-06-03 | 2001-10-04 | Yair Egozy | Core for blood processing apparatus |
| US20060021952A1 (en) * | 2003-09-11 | 2006-02-02 | Skinkle David W | Apparatus for separating blood components |
| CN102061260A (en) * | 2010-12-01 | 2011-05-18 | 汪华 | Medical automatic cell production device and production method thereof |
| CN202131307U (en) * | 2011-06-21 | 2012-02-01 | 中国人民解放军第四军医大学 | Device for separating peripheral blood mononuclear cells |
| CN205974510U (en) * | 2016-06-27 | 2017-02-22 | 江苏伯纳德生物科技发展有限公司 | Multistage blood breakaway connector |
| CN208136228U (en) * | 2018-03-19 | 2018-11-23 | 福建三一造血技术有限公司 | Full-automatic mononuclearcell discrete memory device |
-
2018
- 2018-12-27 CN CN201811608553.XA patent/CN109666580A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20010027156A1 (en) * | 1999-06-03 | 2001-10-04 | Yair Egozy | Core for blood processing apparatus |
| US20060021952A1 (en) * | 2003-09-11 | 2006-02-02 | Skinkle David W | Apparatus for separating blood components |
| CN102061260A (en) * | 2010-12-01 | 2011-05-18 | 汪华 | Medical automatic cell production device and production method thereof |
| CN202131307U (en) * | 2011-06-21 | 2012-02-01 | 中国人民解放军第四军医大学 | Device for separating peripheral blood mononuclear cells |
| CN205974510U (en) * | 2016-06-27 | 2017-02-22 | 江苏伯纳德生物科技发展有限公司 | Multistage blood breakaway connector |
| CN208136228U (en) * | 2018-03-19 | 2018-11-23 | 福建三一造血技术有限公司 | Full-automatic mononuclearcell discrete memory device |
Non-Patent Citations (1)
| Title |
|---|
| 李凌等: "《生物化学与分子生物学实验指导》", 31 August 2015, 人民军医出版社 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110840156A (en) * | 2019-09-23 | 2020-02-28 | 赵树龙 | Intelligent mattress |
| CN113680542A (en) * | 2021-08-25 | 2021-11-23 | 曹莹 | Adipose-derived stem cell collection device |
| CN113680542B (en) * | 2021-08-25 | 2023-12-12 | 湖南同年控股有限公司 | Adipose-derived stem cell collection device |
| CN113684122A (en) * | 2021-09-10 | 2021-11-23 | 江苏省肿瘤医院 | Equipment for rapidly separating and extracting peripheral blood mononuclear cells from blood sample |
| CN114112567A (en) * | 2021-10-29 | 2022-03-01 | 郑州金域临床检验中心有限公司 | Preparation method of tuberculosis infection T cell spot test sample |
| CN115011557A (en) * | 2022-07-18 | 2022-09-06 | 泰州赛特生物医药科技有限公司 | Experimental identification device for non-blood-borne nucleated cells and application method thereof |
| CN115011557B (en) * | 2022-07-18 | 2024-04-26 | 泰州赛特生物医药科技有限公司 | Non-blood-source nucleated cell experimental identification device and using method thereof |
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Application publication date: 20190423 |