CN107790298A - A kind of sampled plasma device and its control method - Google Patents

A kind of sampled plasma device and its control method Download PDF

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Publication number
CN107790298A
CN107790298A CN201610748006.6A CN201610748006A CN107790298A CN 107790298 A CN107790298 A CN 107790298A CN 201610748006 A CN201610748006 A CN 201610748006A CN 107790298 A CN107790298 A CN 107790298A
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CN
China
Prior art keywords
plasma
haemocyte
blood
layering
centrifuge
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CN201610748006.6A
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Chinese (zh)
Inventor
董亚东
王晓宁
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Matches If Mr (shanghai) Biological Technology Co Ltd
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Matches If Mr (shanghai) Biological Technology Co Ltd
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Priority to CN201610748006.6A priority Critical patent/CN107790298A/en
Publication of CN107790298A publication Critical patent/CN107790298A/en
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B5/00Other centrifuges
    • B04B5/04Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B13/00Control arrangements specially designed for centrifuges; Programme control of centrifuges
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B15/00Other accessories for centrifuges
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B5/00Other centrifuges
    • B04B5/12Centrifuges in which rotors other than bowls generate centrifugal effects in stationary containers

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  • Investigating Or Analysing Biological Materials (AREA)
  • External Artificial Organs (AREA)

Abstract

The invention discloses a kind of sampled plasma device and its control method,It is related to medical instruments field,Including centrifuge and the centrifugation cabin for being arranged on centrifuge top half,The Centrifuge Cup for wrapping up connection from inside to outside successively is provided with the centrifugation cabin,Blood plasma is layered and haemocyte layering,The Centrifuge Cup is connected with Centrifuge Cup and enters blood vessel,The blood plasma layering is connected with Centrifuge Cup grout outlet,Haemocyte detector is provided with the middle part of the Centrifuge Cup grout outlet,The Centrifuge Cup grout outlet other end is connected with plasma collecting bag,Alms bowl photoelectric sensor is fixed with the centrifugation cabin inwall,Alms bowl photoelectric sensor is located at before haemocyte detector,In order to lift the quality control level of centrifugal separation collection blood plasma,Reduce blood cell composition in blood plasma collecting bag,The present invention proposes a kind of sampled plasma device and its control method,Blood cell composition in blood plasma collecting bag can be greatly decreased,Lift plasma purity.

Description

A kind of sampled plasma device and its control method
Technical field
The present invention relates to medical instruments field, more particularly to a kind of sampled plasma device and its control method.
Background technology
Blood plasma is a kind of important component in blood of human body, while is also the important source material of some blood products, such as:People Blood albumin, blood albumin of human placenta, human immunoglobulin(HIg) used for intravenous injection, intramuscular injection human immunoglobulin(HIg), histamine people are immunized Globulin, specific immunoglobulin, hepatitis B immunoglobulin, rabies immune globulin, tetanus immune globulin, The blood systems such as human blood coagulation factor VII I, Human Factor Ⅸ Complex, human fibrinogen, Zetbulin Product.
Can not be mixed into according to relevant regulations and requirement, in the raw blood plasma of collection excessive blood cell composition (including:Blood is small The visible components such as plate, leucocyte, red blood cell).In order to which plasma fraction is gathered out, it is necessary to take physics mode to enter from blood Row collection, at present, the acquisition technique of blood plasma can be generally divided into two kinds, respectively centrifugal separation and membrane filter method.The present invention Blood plasma is gathered using centrifugal separation.
Because the consumptive material technical threshold and cost of membrane filter method are higher, so less using the method collection blood in the world Slurry, and more is using centrifugal separation collection blood plasma.
Blood plasma technology is gathered for centrifugal separation, blood is centrifuged after entering the Centrifuge Cup of rotation at a high speed, from Blood after the heart can form haemocyte layering and blood plasma layering, and the blood plasma in blood plasma layering is conveyed successively by Centrifuge Cup grout outlet To blood plasma collecting bag, as the reduction of blood plasma in Centrifuge Cup and the increase of haemocyte, blood cell composition gradually reach grout outlet, it is Prevent the blood cell composition from entering blood plasma collecting bag, domestic common practice:Between Centrifuge Cup grout outlet and blood plasma collecting bag Set a haemocyte detector to carry out detection haemocyte, when haemocyte detector finds haemocyte, stop collection blood plasma, go forward side by side Enter also defeated process, arrive blood donor's human body by the haemocyte in Centrifuge Cup is also defeated.The shortcomings that the method is:Due to haemocyte detector It is limited to the minor constituent detectivity level in haemocyte, when it detects blood cell composition, one in blood cell composition Small component slightly has passed over haemocyte detector and enters blood plasma collecting bag, such as:Blood platelet, leukocyte component etc..So use The method monitoring blood cell composition is limited to the controlled level of plasma purity, there is a large amount of blood platelets and white thin in the blood plasma gathered The blood cell compositions such as born of the same parents.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of sampled plasma device and its control method, existing to solve Caused above-mentioned multinomial defect in technology.
To achieve the above object, the present invention provides following technical scheme:A kind of sampled plasma device and its control method, Including centrifuge and the centrifugation cabin for being arranged on centrifuge top half, it is provided with the centrifugation cabin and wraps from inside to outside successively Centrifuge Cup, blood plasma layering and the haemocyte layering of connection are wrapped up in, the Centrifuge Cup is connected with Centrifuge Cup and enters blood vessel, the blood plasma layering Centrifuge Cup grout outlet is connected with, haemocyte detector, the Centrifuge Cup grout outlet other end are provided with the middle part of the Centrifuge Cup grout outlet Plasma collecting bag is connected with, is fixed with alms bowl photoelectric sensor on the centrifugation cabin inwall, alms bowl photoelectric sensor is located at haemocyte Before detector.
Preferably, be additionally provided with control button on the centrifugation cabin outer wall, the control button using binary-coded decimal switch, Potentiometer or toggle switch.
Preferably, an opening is provided with the centrifuge surface, the opening is provided with drawer.
Preferably, current velocity controller is provided with the middle part of the Centrifuge Cup grout outlet, the current velocity controller is thin in blood Before born of the same parents' detector.
Preferably, the alms bowl photoelectric sensor is used to detect layering circle of haemocyte layering and blood plasma layering in Centrifuge Cup Face, the opportunity for detecting layering interfaces judge that blood plasma adopts slurry amount in advance, and the mode of judgement can be divided into two kinds:Platelet-poor plasma is adopted Integrated mode (PPP patterns) and fresh frozen plasma drainage pattern (FFP patterns).
Preferably, the platelet-poor plasma drainage pattern (PPP patterns):It is needed by alms bowl light during gathering blood plasma Collection capacity when electric transducer and haemocyte detector separately detect haemocyte determines a reference value (difference).
Preferably, the fresh frozen plasma drainage pattern (FFP patterns):Alms bowl photoelectric sensor detects haemocyte layering Be layered with blood plasma after interface gather again it is certain adopt slurry amount, this adopts slurry amount and can be set and be changed by user.
Preferably, if event matches certain condition occurs during adopting slurry, this fresh frozen plasma drainage pattern (FFP Pattern) platelet-poor plasma drainage pattern (PPP patterns) can be converted into, the certain condition is:If alms bowl photoelectric sensor is visited After measuring haemocyte and blood plasma layering interfaces, then the plasma volume gathered also be not up to Y value when haemocyte detector just detect Haemocyte, it will also stop adopting slurry, into also defeated process, meanwhile, reference value X is also determined, subsequent cycle is by according to PPP patterns Control mode is run.
Preferably, the sampled plasma control method also has haemocyte detection mode, and the haemocyte detection mode is protected Stay when only haemocyte detector detects haemocyte or single cycle adopts the maximum collection of single cycle that slurry amount reaches user's setting During amount, just stop adopting slurry, into also defeated process.
Preferably, the alms bowl photoelectric sensor light direct irradiation of the alms bowl photoelectric sensor transmitting is mapped to Centrifuge Cup neck I.e. alms bowl photoelectric sensor particular location is above Centrifuge Cup neck, on centrifugation cabin inwall.
Beneficial effect using above technical scheme is:Structure sampled plasma device of the present invention and its control method, this hair Bright structure sampled plasma device and its control method, using platelet-poor plasma drainage pattern (PPP patterns) and fresh food frozen blood When starching drainage pattern (FFP patterns) collection blood plasma, the method for the collection capacity by prejudging blood plasma in advance, in blood cell composition also not Stop adopting slurry when reaching Centrifuge Cup grout outlet, can effectively prevent blood cell composition and enter blood plasma collecting bag, lifting centrifuges Method gathers the quality control level of blood plasma, ensures plasma purity.Particularly fresh frozen plasma drainage pattern (FFP patterns) is to blood The lifting for starching quality is particularly evident.
Brief description of the drawings
Fig. 1 is the structural representation of sampled plasma device of the present invention and its control method centrifugation cabin.
Fig. 2 is sampled plasma device of the present invention and its front view of control method.
Fig. 3 is sampled plasma device of the present invention and its operating diagram of control method.
Wherein, 1- centrifuges, 2- centrifugation cabins, 3- alms bowl photoelectric sensors, 4- haemocyte detectors, 5- Centrifuge Cups enter blood Pipe, 6- Centrifuge Cup grout outlets, 7- plasma collecting bags, 8-LED LCDs, 9- control buttons, 10- drawers, 11- Centrifuge Cups, 12- alms bowl photoelectric sensor light, the layering of 13- haemocytes, the layering of 14- blood plasma, 15- current velocity controllers.
Embodiment
The preferred embodiment of the invention will now be described in detail with reference to the accompanying drawings sampled plasma device and its control method.
Fig. 1-Fig. 3 shows the embodiment of sampled plasma device of the present invention and its control method:A kind of sampled plasma Device and its control method, including centrifuge 1 and the centrifugation cabin 2 for being arranged on the top half of centrifuge 1, the centrifugation cabin 2 Inside it is provided with the Centrifuge Cup 11 for wrapping up connection from inside to outside successively, blood plasma layering 14 and haemocyte layering 13, the Centrifuge Cup 11 It is connected with Centrifuge Cup and enters blood vessel 5, the blood plasma layering 14 is connected with Centrifuge Cup grout outlet 6, and the middle part of Centrifuge Cup grout outlet 6 is set Haemocyte detector 4 is equipped with, the other end of Centrifuge Cup grout outlet 6 is connected with plasma collecting bag 7.Blood is from Centrifuge Cup when gathering blood plasma Enter blood vessel 5 and enter Centrifuge Cup, enter in Centrifuge Cup and centrifuge, the plasma fraction isolated is entered by Centrifuge Cup grout outlet 6 Enter to blood plasma collecting bag 7.Alms bowl photoelectric sensor 3 is fixed with the centrifugation inwall of cabin 2, it is thin that alms bowl photoelectric sensor 3 is located at blood Before born of the same parents' detector 4.Its alms bowl photoelectric sensor light 12 sent of alms bowl photoelectric sensor 3 is radiated at centrifugation by Centrifuge Cup neck On cup 11, when haemocyte layering 13 is layered 14 interfaces with blood plasma reaches this alms bowl photoelectric sensor 12 point of irradiation of light, due to 13 translucency of haemocyte layering are poor, block the reflection of alms bowl photoelectric sensor light 12, the light quantity that alms bowl photoelectric sensor 3 receives Degree declines, and the output state of alms bowl photoelectric sensor 3 changes, and will output signal to master control system, and master control system receives alms bowl During the output signal of photoelectric sensor 3, then it is assumed that be that alms bowl photoelectric sensor 3 detects haemocyte layering 13 and blood plasma layering 14 Interface.
In the present embodiment, control button 9 is additionally provided with the centrifugation outer wall surface of cabin 2, it is responsible for setting each seed ginseng Number, the control button 9 is using binary-coded decimal switch, potentiometer or toggle switch.
In the present embodiment, an opening is provided with the surface of centrifuge 1, the opening is provided with drawer 10, The medical aid material of some small portables can be stored, adds the storage function of itself.
In the present embodiment, the middle part of Centrifuge Cup grout outlet 6 is provided with current velocity controller 15, the flow control dress 15 are put before haemocyte detector 4, effectively controls the rate of outflow of blood plasma.
The technical scheme of sampled plasma device of the present invention and its control method is as follows:Due to before Centrifuge Cup grout outlet 6 Increase by an alms bowl photoelectric sensor 3, for detecting the layering interfaces of haemocyte layering 13 and blood plasma layering 14 in Centrifuge Cup 11, pass through The opportunity that this sensor detects layering interfaces judges that blood plasma adopts slurry amount in advance, and the mode of judgement can be divided into two kinds:Weary blood platelet Sampled plasma pattern (PPP patterns) and fresh frozen plasma drainage pattern (FFP patterns).
In the present embodiment, the direct irradiation of alms bowl photoelectric sensor light 12 that the alms bowl photoelectric sensor 3 is launched is mapped to The neck of Centrifuge Cup 11 is the particular location of alms bowl photoelectric sensor 3 above the neck of Centrifuge Cup 11, on the centrifugation inwall of cabin 2.
In the present invention, alms bowl photoelectric sensor 3 detect haemocyte layering 13 and blood plasma layering 14 layering interfaces when adopt Integrate amount and difference when haemocyte detector 4 detects haemocyte between collection capacity is reference value X, X is a variable;One program is set Permanent several A, a program setting constant B, a user set variable Y;Reference value X is less than constant B or single cycle is adopted slurry amount and reached During the single cycle maximum collection capacity of user's setting, reference value X is invalid.
Platelet-poor plasma drainage pattern (PPP patterns):Reference value X is determined during slurry in adopting for first circulation, it is next When slurry is adopted in circulation, first judge upper one circulates effective reference value X whether is determined, however, it is determined that effective reference value X, then, In this circulation, after alms bowl photoelectric sensor 3 detects the layering interfaces of haemocyte layering 13 and blood plasma layering 14, then the blood of slurry is adopted Slurry amount is that the reference value X that upper one circulation determines subtracts constant A, and the reference value X that X-A value is circulated as this;If upper one Circulation is without the effective reference value X of determination, then, this circulation will redefine effective reference value X.No matter a upper circulation is It is no that effective reference value X is determined, occur stopping because haemocyte detector 4 detects haemocyte if slurry process is adopted in this circulation When adopting the situation of slurry, subsequent cycle will use the reference value X that this circulation redefines.
Fresh frozen plasma drainage pattern (FFP patterns):When alms bowl photoelectric sensor 3 detects haemocyte layering 13 and blood plasma Layering 14 layering interfaces after, then the plasma volume gathered reach user set variable Y value when, stopping adopt slurry, into also defeated process. After if alms bowl photoelectric sensor 3 detects the layering interfaces of haemocyte layering 13 and blood plasma layering 14, then the plasma volume gathered is also not Haemocyte detector 4 has just detected haemocyte when reaching Y value, will also stop adopting slurry, into also defeated process, meanwhile, also determine Reference value X, subsequent cycle will be run according to the control mode of PPP patterns.
In view of some special circumstances, the present invention also retains the Working mould that haemocyte is only detected with haemocyte detector 4 Formula, i.e., when only haemocyte detector 4 detects haemocyte or single cycle adopts the single cycle maximum that slurry amount reaches user's setting During collection capacity, just stop adopting slurry, into also defeated process.
Based on above-mentioned, structure sampled plasma device of the present invention and its control method, structure sampled plasma device of the present invention and Its control method, adopted using platelet-poor plasma drainage pattern (PPP patterns) and fresh frozen plasma drainage pattern (FFP patterns) When collecting blood plasma, the method for the collection capacity by prejudging blood plasma in advance, stop when blood cell composition does not also reach Centrifuge Cup grout outlet 6 Slurry is only adopted, can effectively prevent blood cell composition and enter blood plasma collecting bag 7, the quality control of lifting centrifugal separation collection blood plasma Level, ensure plasma purity.Particularly lifting of the fresh frozen plasma drainage pattern (FFP patterns) to plasma purity is particularly bright It is aobvious.
Above-described is only the preferred embodiment of the present invention, it is noted that for one of ordinary skill in the art For, without departing from the concept of the premise of the invention, various modifications and improvements can be made, these belong to the present invention Protection domain.

Claims (10)

1. a kind of sampled plasma device and its control method, including centrifuge and the centrifuge for being arranged on centrifuge top half Storehouse, it is characterised in that:It is thin that the Centrifuge Cup for wrapping up connection from inside to outside successively, blood plasma layering and blood are provided with the centrifugation cabin Born of the same parents are layered, and the Centrifuge Cup is connected with Centrifuge Cup and enters blood vessel, and the blood plasma layering is connected with Centrifuge Cup grout outlet, the Centrifuge Cup Haemocyte detector is provided with the middle part of grout outlet, the Centrifuge Cup grout outlet other end is connected with plasma collecting bag, the centrifugation cabin Alms bowl photoelectric sensor is fixed with inwall, alms bowl photoelectric sensor is located at before haemocyte detector.
2. a kind of sampled plasma device according to claim 2 and its control method, it is characterised in that the centrifugation cabin Control button is additionally provided with outer wall, the control button is using binary-coded decimal switch, potentiometer or toggle switch.
3. a kind of sampled plasma device and its control method according to claim 1-3, it is characterised in that the centrifuge An opening is provided with surface, the opening is provided with drawer.
4. a kind of sampled plasma device according to claim 1 and its control method, it is characterised in that the Centrifuge Cup goes out Current velocity controller is provided with the middle part of slurry pipe, the current velocity controller is before haemocyte detector.
A kind of 5. control method of sampled plasma device according to claim 1, it is characterised in that:The alms bowl photoelectric sensing Device is used to detect the layering interfaces of haemocyte layering and blood plasma layering in Centrifuge Cup, and the opportunity for detecting layering interfaces judges in advance Blood plasma adopts slurry amount, and the mode of judgement can be divided into two kinds:Platelet-poor plasma drainage pattern and fresh frozen plasma drainage pattern.
A kind of 6. control method of sampled plasma device according to claim 5, it is characterised in that:The weary blood platelet blood Starch drainage pattern:It needs to separately detect haemocyte by alms bowl photoelectric sensor and haemocyte detector during gathering blood plasma When collection capacity determine a reference value.
A kind of 7. control method of sampled plasma device according to claim 5, it is characterised in that:The fresh food frozen blood Starch drainage pattern (FFP patterns):Alms bowl photoelectric sensor gathers necessarily again after detecting haemocyte layering and blood plasma layering interface Adopt slurry amount, this adopts slurry amount and can be set and be changed by user.
A kind of 8. control method of sampled plasma device according to claim 5, it is characterised in that:If during adopting slurry During generation event matches certain condition, this fresh frozen plasma drainage pattern can be converted into platelet-poor plasma drainage pattern, The certain condition is:After if alms bowl photoelectric sensor detects haemocyte and blood plasma layering interfaces, then the plasma volume gathered is also not Haemocyte detector has just detected haemocyte when reaching Y value, will also stop adopting slurry, into also defeated process;Meanwhile also determine Reference value X, subsequent cycle will be run according to the control mode of PPP patterns.
A kind of 9. control method of sampled plasma device according to claim 5-8, it is characterised in that:The sampled plasma Control method also has haemocyte detection mode, when the haemocyte detection mode is that only haemocyte detector detects haemocyte Or single cycle adopt slurry amount reach user setting single cycle maximum collection capacity when, just stopping adopt slurry, into also defeated process.
10. a kind of sampled plasma device according to claim 1, it is characterised in that the alms bowl photoelectric sensor transmitting Alms bowl photoelectric sensor light direct irradiation is mapped to Centrifuge Cup neck i.e. alms bowl photoelectric sensor particular location on Centrifuge Cup neck In side, centrifugation cabin inwall.
CN201610748006.6A 2016-08-29 2016-08-29 A kind of sampled plasma device and its control method Pending CN107790298A (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109142013A (en) * 2018-07-18 2019-01-04 中国人民解放军陆军军医大学第附属医院 A kind of separation method of blood plasma chylomicron
CN109395893A (en) * 2018-11-30 2019-03-01 江苏伯纳德生物科技发展有限公司 A kind of cup type centrifugal device being suitable for different disposal blood volume
CN110124880A (en) * 2019-05-23 2019-08-16 四川南格尔生物科技有限公司 A kind of centrifugation cup structure and haemocyte washing and separation method
CN111992339A (en) * 2020-07-28 2020-11-27 刘肖琳 Centrifugal separation device, and method for separating and culturing mixed liquid
CN115007331A (en) * 2022-04-21 2022-09-06 菲尔德森(江苏)生物技术有限公司 Blood component separator and method of use
WO2023019611A1 (en) * 2021-08-16 2023-02-23 赛若麦得(上海)生物技术有限公司 High-reliability cell detector

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CN2703526Y (en) * 2004-04-06 2005-06-08 山东威高集团医用高分子制品股份有限公司 Blood-cell separator
CN104491945A (en) * 2014-12-31 2015-04-08 成都市佳颖医用制品有限公司 Blood plasma collecting device
CN106267426A (en) * 2016-08-15 2017-01-04 董稳 A kind of centrifugal separation sampled plasma device

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Publication number Priority date Publication date Assignee Title
CN2393550Y (en) * 1999-09-09 2000-08-30 浙江博朗生物工程有限公司 Automatic blood separator
CN2394222Y (en) * 1999-09-09 2000-08-30 浙江博朗生物工程有限公司 Centrifuger for blood separation
CN2703526Y (en) * 2004-04-06 2005-06-08 山东威高集团医用高分子制品股份有限公司 Blood-cell separator
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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109142013A (en) * 2018-07-18 2019-01-04 中国人民解放军陆军军医大学第附属医院 A kind of separation method of blood plasma chylomicron
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CN109395893A (en) * 2018-11-30 2019-03-01 江苏伯纳德生物科技发展有限公司 A kind of cup type centrifugal device being suitable for different disposal blood volume
CN110124880A (en) * 2019-05-23 2019-08-16 四川南格尔生物科技有限公司 A kind of centrifugation cup structure and haemocyte washing and separation method
CN111992339A (en) * 2020-07-28 2020-11-27 刘肖琳 Centrifugal separation device, and method for separating and culturing mixed liquid
CN111992339B (en) * 2020-07-28 2022-02-11 刘肖琳 Centrifugal separation device, and method for separating and culturing mixed liquid
WO2023019611A1 (en) * 2021-08-16 2023-02-23 赛若麦得(上海)生物技术有限公司 High-reliability cell detector
CN115007331A (en) * 2022-04-21 2022-09-06 菲尔德森(江苏)生物技术有限公司 Blood component separator and method of use

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