CN114540172A - Biological deodorization bacterium culture device and method - Google Patents

Biological deodorization bacterium culture device and method Download PDF

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Publication number
CN114540172A
CN114540172A CN202210177931.3A CN202210177931A CN114540172A CN 114540172 A CN114540172 A CN 114540172A CN 202210177931 A CN202210177931 A CN 202210177931A CN 114540172 A CN114540172 A CN 114540172A
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hole
guide pipe
block
fixed
fermentation
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高亚飞
吕相令
韩立
薛中华
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Anhui Yangdi Ecological Technology Co ltd
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Anhui Yangdi Ecological Technology Co ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04Seals
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • General Health & Medical Sciences (AREA)
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Abstract

One or more embodiments of the specification provide a biological deodorization bacteria culture apparatus and method, which relate to the field of deodorization bacteria and comprise a plurality of fermentation tanks, wherein the top ends of the fermentation tanks are provided with first through holes; the sealing blocks are matched with the first through holes and used for sealing the corresponding first through holes when the samples are not taken; the front end of the guide pipe is fixed on the top end surface of the sealing block, and a plurality of second through holes communicated with the interior of the guide pipe are formed in the side surface, close to the front end, of the guide pipe; the cylinder is fixed on the ground, and an output shaft of the cylinder is connected with the guide pipe; the fermentation tank comprises a collecting box and an air pump, wherein the inside of the collecting box is provided with storage cavities which correspond to the fermentation tanks one by one, the storage cavities are mutually independent, the top of each storage cavity is provided with a fourth through hole, and the diameter of the fourth through hole is equal to the outer diameter of a guide pipe; after the collecting box is placed on the ground, the fourth through hole is positioned right below the rear end of the corresponding guide pipe, and the air suction pump is communicated with the storage cavities through the guide pipes. By the invention, sampling of each fermentation tank can be completed quickly, and sampling time is saved.

Description

Biological deodorization bacterium culture device and method
Technical Field
One or more embodiments of the present disclosure relate to the field of deodorant bacteria, and more particularly, to a biological deodorant bacteria culture apparatus and method.
Background
The biological deodorization method has the advantages of being superior to high efficiency, environment-friendly and the like, is increasingly used for deodorization in livestock and poultry breeding, has wide market popularization prospect, adopts deodorization bacteria for deodorization in biological deodorization, and needs to pay attention to the conditions of flora in the fermentation process in the process of producing and culturing the deodorization bacteria in factories, so that a certain amount of fermentation liquor needs to be extracted for observation in the fermentation process.
The applicant found that: in mass production of deodorant bacteria, since a plurality of fermentation tanks are required, it takes a lot of time to sample one by one.
Disclosure of Invention
In view of the above, one or more embodiments of the present disclosure are directed to a culture apparatus and method for biological deodorant bacteria, which can solve the problem of the prior art that a lot of time is required for sampling the deodorant bacteria in one sampling because a plurality of fermentation tanks are required for mass production of the deodorant bacteria.
In view of the above objects, one or more embodiments of the present specification provide a culture device for bio-deodorant bacteria, the culture device including:
the fermentation tanks are fixed on the ground, and the top ends of the fermentation tanks are provided with first through holes;
the sealing block is matched with the first through hole and used for sealing the corresponding first through hole when no sample is taken;
the front end of the guide pipe is fixed on the top end surface of the sealing block, and a plurality of second through holes communicated with the interior of the guide pipe are formed in the side surface, close to the front end, of the guide pipe;
the output shaft of the cylinder is connected with the guide pipe and is used for driving the guide pipe to move upwards or downwards;
the fermentation tank comprises a collection box and an air pump fixed at the top of the collection box, wherein the interior of the collection box is provided with storage cavities which correspond to the fermentation tanks one by one, the storage cavities are mutually independent, the top of each storage cavity is provided with a fourth through hole, and the diameter of the fourth through hole is equal to the outer diameter of a guide pipe; after the collecting box is placed on the ground, the fourth through hole is positioned right below the rear end of the corresponding guide pipe, and the air suction pump is communicated with the storage cavities through the guide pipes.
Further, the culture apparatus further comprises: the plug block is matched with the fourth through hole, the elastic pull rope is located in the storage cavity, one end of the elastic pull rope is fixed to the bottom end of the plug block, the other end of the elastic pull rope is fixed to the top end face of the storage cavity, the rear end face of the guide pipe is sealed, and a fifth through hole is formed in the side surface, close to the rear end face, of the guide pipe.
Further, the culture apparatus further comprises: the bottom end of the fixed cylinder is fixed on the top end face of the fermentation tank, the limiting block is arranged on the inner wall of the fixed cylinder, and the fixed block is fixed on the outer side face of the guide pipe; when the top end face of the sealing block is contacted with the limiting block, the rear end of the guide pipe is separated from the fourth through hole; when the bottom end face of the fixing block is contacted with the limiting block, the rear end of the guide pipe extends into the storage cavity.
Further, culture apparatus still includes solenoid valve and first water pump, the fixed block is equipped with two third through-holes that run through its upper and lower terminal surface, be equipped with in the third through-hole the solenoid valve, the solenoid valve is used for controlling the break-make of third through-hole, one of them third through-hole with the delivery port intercommunication of first water pump, the water inlet of first water pump leads to pipe and cask intercommunication, and is equipped with the pure water in this cask.
Further, the culture apparatus further comprises:
the linkage block is provided with a through hole penetrating through the upper surface and the lower surface of the linkage block, the diameter of the through hole is equal to the outer diameter of the catheter, the rear end of the catheter penetrates through the through hole, and the side wall of the through hole is provided with a side hole penetrating through the outer side surface of the linkage block;
the first side block is fixed on the outer side surface of the guide pipe, one end of the first spring is fixed on the first side block, the other end of the first spring is fixed on the upper end surface of the linkage block, when the bottom end surface of the linkage block is separated from the collecting box, the fifth through hole enters the through hole, and the fifth through hole is positioned below the side hole;
and the water inlet of the second water pump is communicated with the side hole through a water pipe.
Further, the culture apparatus further comprises:
the lower end face of the top plate is fixedly connected with an output shaft of the air cylinder;
the second side block is fixed on the side surface of the catheter, the stop block is positioned above the first side block, and the second side block is positioned above the stop block;
one end of the second spring is fixedly connected with the second side block, and the other end of the second spring is fixed on the upper end face of the top plate;
one end of the connecting rod is fixedly connected with the outer side surface of the linkage block, and the connecting rod penetrates through a sixth through hole formed in the top plate;
and the fixed separation assembly is arranged in the sixth through hole and is used for fixing or separating the connecting rod and the top plate.
Further, the fixed separating assembly comprises:
the inner groove is arranged in the top plate and penetrates through the sixth through hole, and the inner groove comprises a front groove positioned on one side of the sixth through hole and a rear groove positioned on the other side of the sixth through hole;
the first electromagnet is fixed at the bottom of the rear groove;
the cross rod is connected with the inner groove in a sliding manner, and the second electromagnet is arranged in the cross rod;
the connecting rod is provided with a surface hole penetrating through the side surface thereof.
A method for culturing biological deodorization bacteria adopts the biological deodorization bacteria culture device, and the use method comprises the following steps:
the method comprises the following steps: before fermentation of the fermentation tank, the sealing block is positioned in the first through hole through the air cylinder, and the first through hole is sealed;
step two: putting fermentation substances required by the deodorant bacteria into a fermentation tank for fermentation;
step three: in the fermentation process, the collecting box is placed on the ground, so that the fourth through hole is positioned right below the rear end of the corresponding guide pipe;
step four: the cylinder is started to drive the guide pipe to move downwards, when the rear end of the guide pipe passes through the fourth through hole and enters the corresponding storage cavity, the fourth through hole at the front end of the guide pipe enters the fermentation liquid;
step five: starting the air pump to generate suction in each storage cavity, so that a part of fermentation liquor flows through the conduit to enter the storage cavity to finish sampling;
step six: the cylinder is restarted so that the sealing block reseals the first through hole.
The invention has the beneficial effects that: the invention relates to a culture device and a method for biological deodorization bacteria, wherein before fermentation of the deodorization bacteria, a sealing block seals a first through hole through an air cylinder, after the fermentation is finished, fermentation substances required by the deodorization bacteria are placed in each fermentation tank, and then fermentation is started, sampling is carried out after a certain time in the fermentation process so as to observe the deodorization bacteria condition in each fermentation tank, after the time required for sampling is reached, a collecting box is firstly placed on the ground, a fourth through hole is positioned right below the rear end of a corresponding guide pipe, then the air cylinder is started to drive the guide pipe to move downwards, when the rear end of the guide pipe passes through the fourth through hole and enters a corresponding storage cavity, a fourth through hole at the front end of the guide pipe enters the fermentation liquid, after the sampling is finished, an air pump is started to enable each storage cavity to generate suction, so that a part of the fermentation liquid flows through the guide pipe and enters the storage cavity, therefore, sampling is completed, after the sampling is completed, the air suction pump is powered off, then the air cylinder is started again, the sealing block enables the first through hole to be sealed again, the rear end of the guide pipe is separated from the fourth through hole at the moment, then the collecting box is taken out, and workers can take out fermentation liquor in the storage cavity in sequence to observe the state of the deodorant bacteria.
Drawings
In order to more clearly illustrate one or more embodiments or prior art solutions of the present specification, the drawings that are needed in the description of the embodiments or prior art will be briefly described below, and it is obvious that the drawings in the following description are only one or more embodiments of the present specification, and that other drawings may be obtained by those skilled in the art without inventive effort from these drawings.
FIG. 1 is a schematic structural diagram of an embodiment of the present invention;
FIG. 2 is an enlarged view of a stationary barrel in an embodiment of the present invention;
FIG. 3 is an enlarged view of a collection tank in an embodiment of the present invention;
FIG. 4 is an enlarged view of the periphery of a cylinder in an embodiment of the present invention;
fig. 5 is a schematic structural diagram of a top plate in an embodiment of the present invention.
Wherein, 1, a fermentation tank; 2. a fixed cylinder; 3. a conduit; 5. a collection box; 6. a storage chamber; 7. an air pump; 9. a cylinder; 10. a top plate; 11. a first through hole; 12. sealing blocks; 13. a limiting block; 14. a second through hole; 15. a fixed block; 16. a third through hole; 17. an electromagnetic valve; 18. a fourth via hole; 19. a chock block; 20. an elastic pull rope; 21. a linkage block; 22. a fifth through hole; 23. a through hole; 24. a side hole; 25. a first side block; 26. a first spring; 27. a stopper; 28. a second side block; 29. a second spring; 30. a connecting rod; 31. a sixth through hole; 32. an inner tank; 33. a first electromagnet; 34. a cross bar; 35. a second electromagnet; 36. surface pores.
Detailed Description
To make the objects, technical solutions and advantages of the present disclosure more apparent, the present disclosure is further described in detail below with reference to specific embodiments.
It is to be noted that unless otherwise defined, technical or scientific terms used in one or more embodiments of the present specification should have the ordinary meaning as understood by those of ordinary skill in the art to which this disclosure belongs. The use of "first," "second," and similar terms in one or more embodiments of the specification is not intended to indicate any order, quantity, or importance, but rather is used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that the element or item listed before the word covers the element or item listed after the word and its equivalents, but does not exclude other elements or items. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly.
In view of the above object, according to a first aspect of the present invention, there is provided an embodiment of a culture apparatus for bio-deodorant bacteria, as shown in fig. 1, 2 and 3, comprising:
a plurality of fermentation tanks 1 fixed on the ground, wherein the top ends of the fermentation tanks 1 are provided with first through holes 11;
the sealing blocks 12 are matched with the first through holes 11, and the sealing blocks 12 are used for sealing the corresponding first through holes 11 when sampling is not carried out;
the front end of the conduit 3 is fixed on the top end surface of the sealing block 12, and a plurality of second through holes 14 communicated with the interior of the conduit 3 are formed in the side surface, close to the front end, of the conduit 3;
the cylinder 9 is fixed on the ground, and an output shaft of the cylinder 9 is connected with the guide pipe 3 and used for driving the guide pipe 3 to move upwards or downwards;
the fermentation tank comprises a collecting box 5 and an air suction pump 7 fixed at the top of the collecting box 5, wherein the inside of the collecting box 5 is provided with storage cavities 6 corresponding to the fermentation tanks 1 one by one, the storage cavities 6 are mutually independent, the top of each storage cavity 6 is provided with a fourth through hole 18, and the diameter of each fourth through hole 18 is equal to the outer diameter of a guide pipe 3; after the collection box 5 is placed on the ground, the fourth through hole 18 is located right below the rear end of the corresponding conduit 3, and the suction pump 7 is communicated with each storage cavity 6 through the conduit 3.
In this embodiment, before fermentation of the deodorant bacteria, the sealing block 12 seals the first through hole 11 through the cylinder 9, after the fermentation is completed, the fermentation material required by the deodorant bacteria is placed in each fermentation tank 1, and then fermentation is started, during the fermentation process, sampling is performed after a certain time to observe the condition of the deodorant bacteria in each fermentation tank 1, after the time required for sampling is reached, the collection tank 5 is first placed on the ground, the fourth through hole 18 is located right below the rear end of the corresponding conduit 3, then the cylinder 9 is started to drive the conduit 3 to move downwards, when the rear end of the conduit 3 passes through the fourth through hole 18 and enters the corresponding storage cavity 6, the fourth through hole 18 at the front end of the conduit 3 enters the interior of the fermentation liquid, after the completion, the air suction pump 7 is started to generate suction in each storage cavity 6, so that a part of the fermentation liquid flows through the conduit 3 and enters the storage cavity 6, therefore, sampling is finished, after the sampling is finished, the air suction pump 7 is powered off, then the air cylinder 9 is started again, the sealing block 12 seals the first through hole 11 again, at the moment, the rear end of the guide pipe 3 is separated from the fourth through hole 18, then the collecting box 5 is taken out, and workers can take out fermentation liquor in the storage cavity 6 in sequence to observe the state of the deodorant bacteria.
In one embodiment, as shown in fig. 3 and 4, the culture apparatus further includes: the plug 19 is matched with the fourth through hole 18, the elastic pull rope 20 is positioned in the storage cavity 6, one end of the elastic pull rope 20 is fixed at the bottom end of the plug 19, the other end of the elastic pull rope 20 is fixed at the top end face of the storage cavity 6, the rear end face of the guide pipe 3 is sealed, and the side surface of the guide pipe 3 close to the rear end face is provided with a fifth through hole 22.
In this embodiment, when the rear end of the guide tube 3 is separated from the fourth through hole 18, the fourth through hole 18 will be sealed by the stopper 19 under the pulling force of the elastic pulling rope 20, so that the air in the external environment is prevented from entering the storage cavity 6 and polluting the sample during the process of transferring the collection box 5.
In one embodiment, as shown in fig. 1 and 2, the culture apparatus further includes: the bottom mounting is in fixed cylinder 2 of fermentation cylinder 1 top terminal surface, locate stopper 13 of fixed cylinder 2 inner wall and be fixed in the fixed block 15 of pipe 3 lateral surface, and the diameter of first through-hole 11 equals with the internal diameter of fixed cylinder 2, and the axis of first through-hole 11 coincides with the axis of fixed cylinder 2, and fixed cylinder 2 is passed to the front end of pipe 3, fixed block 15 and the inner wall sliding connection of fixed cylinder 2.
In the present embodiment, when the top end surface of the seal block 12 contacts the stopper 13, the rear end of the guide tube 3 is separated from the fourth through hole 18; when the bottom end face of the fixing block 15 contacts the stopper 13, the rear end of the guide tube 3 extends into the storage chamber 6.
As an implementation mode, as shown in fig. 1 and 2, the culture apparatus further includes an electromagnetic valve 17 and a first water pump, the fixed block 15 is provided with two third through holes 16 penetrating through upper and lower end surfaces thereof, the electromagnetic valve 17 is arranged in the third through hole 16, the electromagnetic valve 17 is used for controlling the on-off of the third through hole 16, one of the third through holes 16 is communicated with a water outlet of the first water pump, a water inlet of the first water pump is communicated with a water tank through a water pipe, and the water tank is filled with purified water.
In this embodiment, in the sampling process, the two electromagnetic valves 17 respectively close the corresponding third through holes 16, after sampling is completed, the two electromagnetic valves 17 respectively open the corresponding third through holes 16, then the first water pump is started, so that water is filled in the fixed cylinder 2, at this time, the electromagnetic valves 17 in the third through holes 16 which are not communicated with the first water pump close the third through holes 16, so that the water enters the conduit 3 and flows out of the conduit 3, and thus, the conduit 3 is cleaned.
In one embodiment, as shown in fig. 1 and 4, the culture apparatus further includes:
the linkage block 21, the linkage block 21 has through hole 23 which runs through its upper and lower surfaces, the diameter of the through hole 23 is equal to the external diameter of the conduit 3, and the rear end of the conduit 3 runs through the through hole 23, the sidewall of the through hole 23 has side hole 24 which runs through the lateral surface of the linkage block 21;
a first side block 25 fixed on the outer side surface of the conduit 3, and a first spring 26 with one end fixed on the first side block 25, wherein the other end of the first spring 26 is fixed on the upper end surface of the linkage block 21, when the bottom end surface of the linkage block 21 is separated from the collecting box 5, the fifth through hole 22 enters the through hole 23, and the fifth through hole 22 is positioned below the side hole 24;
and a water inlet of the second water pump is communicated with the side hole 24 through a water pipe.
In this embodiment, when the bottom end surface of the linkage block 21 is separated from the collection box 5, the fifth through hole 22 enters the through hole 23, and the fifth through hole 22 is located below the side hole 24, so that it can be avoided that external air enters the conduit 3 when no sampling occurs, thereby polluting the internal environment of the conduit 3, when sampling occurs, the rear end of the conduit 3 is driven to move downward by the cylinder 9, in the process of downward movement of the rear end of the conduit 3, the lower end surface of the linkage block 21 contacts the upper end surface of the collection box 5, and when the conduit 3 continues to move downward, the conduit 3 can inevitably overcome the elastic force of the first spring 26, thereby gradually entering the fifth through hole 22 into the storage cavity 6; after the sample is accomplished, before first water pump washs, through external force for side opening 24 is just to fifth through-hole 22, then first water pump washs, washs the completion back, and the second water pump starts, takes out remaining water in pipe 3 and the solid fixed cylinder 2.
In one embodiment, as shown in FIG. 1, FIG. 4, and FIG. 5, the culture apparatus further includes:
the lower end face of the top plate 10 is fixedly connected with an output shaft of the cylinder 9;
a second side block 28 and a stopper 27 fixed to a side surface of the guide tube 3, the stopper 27 being positioned above the first side block 25, the second side block 28 being positioned above the stopper 27;
a second spring 29 having one end fixedly connected to the second side block 28, and the other end of the second spring 29 being fixed to the upper end surface of the top plate 10;
a connecting rod 30 with one end fixedly connected with the outer side surface of the linkage block 21, wherein the connecting rod 30 passes through a sixth through hole 31 arranged on the top plate 10;
and a fixing and separating assembly provided in the sixth through hole 31 for fixing or separating the connecting rod 30 to or from the top plate 10.
In this embodiment, before the first water pump is cleaned, the fixing and separating assembly separates the connecting rod 30 from the top plate 10, so that when the cylinder 9 is started, the guide tube 3 is driven by the top plate 10 to move, and here, after the guide tube 3 moves downwards and the linkage block 21 contacts the collecting box 5, the top plate 10 further moves downwards against the contraction force of the second spring 29, at this time, the edge of the top plate 10 will contact with the stopper 27, so as to avoid the second spring 29 from being damaged, when the water tube needs to be cleaned, the fixing and separating assembly fixes the connecting rod 30 to the top plate 10, so that the cylinder 9 is started, the linkage block 21 is driven by the connecting rod 30 to move upwards for a certain distance, at this time, because the sealing block 12 contacts with the stopper 13, the top plate 10 will only compress the second spring 29, when the side hole 24 is opposite to the fifth through hole 22, the cylinder 9 stops driving, at this point, cleaning can be performed.
As an embodiment, as shown in fig. 4 and 5, the fixed separating assembly includes:
an inner groove 32 provided in the top plate 10, the inner groove 32 penetrating the sixth through hole 31, the inner groove 32 including a front groove located at one side of the sixth through hole 31 and a rear groove located at the other side of the sixth through hole 31;
a first electromagnet 33 fixed to the bottom of the rear groove;
a cross bar 34 slidably connected to the inner groove 32 and a second electromagnet 35 disposed in the cross bar 34;
the connecting rod 30 is provided with a surface hole 36 penetrating through a side surface thereof.
In this embodiment, when the fifth through hole 22 is contracted in the through hole 23 and the surface hole 36 crosses the inner groove 32, the first and second electromagnets 33 and 35 generate a force of attraction to each other so that a portion of the connecting rod 30 enters the rear groove, and at this time, the connecting rod 30 and the top plate 10 move in the same state, and when the first and second electromagnets 33 and 35 generate a force of repulsion to each other so that the connecting rod 30 completely enters the front groove, the connecting rod 30 is separated from the top plate 10.
A method for culturing biological deodorization bacteria adopts the biological deodorization bacteria culture device, and the use method comprises the following steps:
the method comprises the following steps: before fermentation of the fermentation tank 1, the sealing block 12 is positioned in the first through hole 11 through the air cylinder 9, and the first through hole 11 is sealed;
step two: putting fermentation substances required by the deodorant bacteria into a fermentation tank 1 for fermentation;
step three: during the fermentation process, the collection box 5 is placed on the ground, so that the fourth through hole 18 is positioned right below the rear end of the corresponding conduit 3;
step four: the cylinder 9 is started to drive the guide pipe 3 to move downwards, when the rear end of the guide pipe 3 passes through the fourth through hole 18 and enters the corresponding storage cavity 6, the fourth through hole 18 at the front end of the guide pipe 3 enters the fermentation liquid;
step five: the air pump 7 is started to generate suction in each storage cavity 6, so that a part of fermentation liquor flows through the conduit 3 into the storage cavities 6 to finish sampling;
step six: the cylinder 9 is activated again so that the sealing block 12 seals the first through hole 11 again.
Those of ordinary skill in the art will understand that: the discussion of any embodiment above is meant to be exemplary only, and is not intended to intimate that the scope of the disclosure, including the claims, is limited to these examples; within the spirit of the present disclosure, features from the above embodiments or from different embodiments may also be combined, steps may be implemented in any order, and there are many other variations of different aspects of one or more embodiments of the present description as described above, which are not provided in detail for the sake of brevity.
It is intended that the one or more embodiments of the present specification embrace all such alternatives, modifications and variations as fall within the broad scope of the appended claims. Therefore, any omissions, modifications, substitutions, improvements, and the like that may be made without departing from the spirit and principles of one or more embodiments of the present disclosure are intended to be included within the scope of the present disclosure.

Claims (8)

1. A culture device for biological deodorization bacteria, comprising:
the fermentation tanks (1) are fixed on the ground, and the top ends of the fermentation tanks (1) are provided with first through holes (11);
the sealing block (12) is matched with the first through hole (11), and the sealing block (12) is used for sealing the corresponding first through hole (11) when a sample is not taken;
the front end of the guide pipe (3) is fixed on the top end surface of the sealing block (12), and a plurality of second through holes (14) communicated with the interior of the guide pipe (3) are formed in the side surface, close to the front end, of the guide pipe (3);
the air cylinder (9) is fixed on the ground, and an output shaft of the air cylinder (9) is connected with the guide pipe (3) and used for driving the guide pipe (3) to move upwards or downwards;
the fermentation tank comprises a collection box (5) and an air suction pump (7) fixed at the top of the collection box (5), wherein the interior of the collection box (5) is provided with storage cavities (6) which correspond to the fermentation tanks (1) one by one, the storage cavities (6) are mutually independent, the top of each storage cavity (6) is provided with a fourth through hole (18), and the diameter of each fourth through hole (18) is equal to the outer diameter of a guide pipe (3); after the collecting box (5) is placed on the ground, the fourth through hole (18) is located under the rear end of the corresponding guide pipe (3), and the air suction pump (7) is communicated with the storage cavities (6) through the guide pipe (3).
2. The culture device of claim 1, further comprising: the plug block (19) is matched with the fourth through hole (18), the elastic pull rope (20) is located in the storage cavity (6), one end of the elastic pull rope (20) is fixed to the bottom end of the plug block (19), the other end of the elastic pull rope (20) is fixed to the top end face of the storage cavity (6), the rear end face of the guide pipe (3) is sealed, and a fifth through hole (22) is formed in the side surface, close to the rear end face, of the guide pipe (3).
3. The culture device of claim 2, further comprising: the bottom end of the fermentation tank is fixed on a fixed cylinder (2) of the top end face of the fermentation tank (1), a limiting block (13) arranged on the inner wall of the fixed cylinder (2) and a fixing block (15) fixed on the outer side face of the guide pipe (3), the diameter of the first through hole (11) is equal to the inner diameter of the fixed cylinder (2), the central axis of the first through hole (11) is superposed with the central axis of the fixed cylinder (2), the front end of the guide pipe (3) penetrates through the fixed cylinder (2), and the fixing block (15) is connected with the inner wall of the fixed cylinder (2) in a sliding mode; when the top end face of the sealing block (12) is in contact with the limiting block (13), the rear end of the guide pipe (3) is separated from the fourth through hole (18); when the bottom end face of the fixing block (15) is in contact with the limiting block (13), the rear end of the guide pipe (3) extends into the storage cavity (6).
4. The culture apparatus of claim 3, further comprising an electromagnetic valve (17) and a first water pump, wherein the fixed block (15) is provided with two third through holes (16) penetrating through the upper and lower end surfaces of the fixed block, the electromagnetic valve (17) is arranged in the third through hole (16), the electromagnetic valve (17) is used for controlling the on-off of the third through hole (16), one of the third through holes (16) is communicated with the water outlet of the first water pump, the water inlet of the first water pump is communicated with a water barrel through a water pipe, and purified water is filled in the water barrel.
5. The culture device of claim 4, further comprising:
the linkage block (21) is provided with a through hole (23) penetrating through the upper surface and the lower surface of the linkage block (21), the diameter of the through hole (23) is equal to the outer diameter of the catheter (3), the rear end of the catheter (3) penetrates through the through hole (23), and the side wall of the through hole (23) is provided with a side hole (24) penetrating through the outer side surface of the linkage block (21);
the first side block (25) is fixed on the outer side surface of the guide pipe (3), one end of the first spring (26) is fixed on the first side block (25), the other end of the first spring (26) is fixed on the upper end surface of the linkage block (21), when the bottom end surface of the linkage block (21) is separated from the collection box (5), the fifth through hole (22) enters the through hole (23), and the fifth through hole (22) is positioned below the side hole (24);
and a water inlet of the second water pump is communicated with the side hole (24) through a water pipe.
6. The culture device of claim 5, further comprising:
the lower end face of the top plate (10) is fixedly connected with an output shaft of the cylinder (9);
a second side block (28) and a stopper (27) fixed to the side surface of the guide tube (3), the stopper (27) being positioned above the first side block (25), the second side block (28) being positioned above the stopper (27);
a second spring (29) with one end fixedly connected with the second side block (28), and the other end of the second spring (29) is fixed on the upper end surface of the top plate (10);
one end of the connecting rod (30) is fixedly connected with the outer side face of the linkage block (21), and the connecting rod (30) penetrates through a sixth through hole (31) formed in the top plate (10);
and the fixing and separating assembly is arranged in the sixth through hole (31) and is used for fixing or separating the connecting rod (30) and the top plate (10).
7. The culture apparatus of claim 6, wherein the stationary separation assembly comprises:
the inner groove (32) is arranged in the top plate (10), the inner groove (32) penetrates through the sixth through hole (31), and the inner groove (32) comprises a front groove positioned on one side of the sixth through hole (31) and a rear groove positioned on the other side of the sixth through hole (31);
a first electromagnet (33) fixed to the bottom of the rear groove;
a cross bar (34) connected with the inner groove (32) in a sliding way and a second electromagnet (35) arranged in the cross bar (34);
the connecting rod (30) is provided with a surface hole (36) penetrating through a side surface thereof.
8. A method for culturing the biological deodorizing bacteria, characterized by comprising the use of the culture apparatus for the biological deodorizing bacteria according to claim 1, wherein the culture apparatus for the biological deodorizing bacteria is used in the following manner:
the method comprises the following steps: before fermentation of the fermentation tank (1), a sealing block (12) is positioned in the first through hole (11) through the air cylinder (9) to seal the first through hole (11);
step two: putting fermentation substances required by the deodorant bacteria into a fermentation tank (1) for fermentation;
step three: during the fermentation process, the collecting box (5) is placed on the ground, so that the fourth through hole (18) is positioned right below the rear end of the corresponding conduit (3);
step four: the cylinder (9) is started to drive the guide pipe (3) to move downwards, when the rear end of the guide pipe (3) passes through the fourth through hole (18) and enters the corresponding storage cavity (6), the fourth through hole (18) at the front end of the guide pipe (3) enters the fermentation liquid;
step five: the air pump (7) is started to generate suction in each storage cavity (6), so that a part of fermentation liquor flows through the conduit (3) into the storage cavity (6) to finish sampling;
step six: the cylinder (9) is activated again, so that the sealing block (12) seals the first through hole (11) again.
CN202210177931.3A 2022-02-25 2022-02-25 Biological deodorization bacterium culture device and method Pending CN114540172A (en)

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CN202210177931.3A CN114540172A (en) 2022-02-25 2022-02-25 Biological deodorization bacterium culture device and method

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Application Number Priority Date Filing Date Title
CN202210177931.3A CN114540172A (en) 2022-02-25 2022-02-25 Biological deodorization bacterium culture device and method

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115193237A (en) * 2022-09-14 2022-10-18 山东天弘化学有限公司 Hydrogenation reaction device and use method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115193237A (en) * 2022-09-14 2022-10-18 山东天弘化学有限公司 Hydrogenation reaction device and use method thereof
CN115193237B (en) * 2022-09-14 2022-11-22 山东天弘化学有限公司 Hydrogenation reaction device and use method thereof

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