CN106305631A - Breeding method of high-protein Hermetia illucens pupae - Google Patents
Breeding method of high-protein Hermetia illucens pupae Download PDFInfo
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- CN106305631A CN106305631A CN201610721560.5A CN201610721560A CN106305631A CN 106305631 A CN106305631 A CN 106305631A CN 201610721560 A CN201610721560 A CN 201610721560A CN 106305631 A CN106305631 A CN 106305631A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention relates to a breeding method of high-protein Hermetia illucens pupae, and belongs to the technical field of breeding. The breeding method comprises the following steps: prompting Hermetia illucens male pupae and female pupae to lay eggs; collecting Hermetia illucens eggs, and breeding to incubate larvae; breeding the larvae with kitchen waste; after breeding, cleaning; putting the bred larvae into a culture dish; adding substances such as peptone, discarded dead fish and corn flour; breeding at a controlled temperature; adding the peptone and skim milk every day till the breeding reaches a pre-pupa stage; collecting husked Hermetia illucens pupae; performing cryopreservation to obtain the high-protein Hermetia illucens pupae. The Hermetia illucens pupae bred by the method are 65 percent or above in protein content, are rich in nutrition, and have a wide application value on the aspects of facial care and foods; the breeding method has a high survival rate, and is easy to operate.
Description
Technical field
The present invention relates to the cultural method of a kind of high protein black soldier flies pupa, belong to cultural technique field.
Background technology
The dry worm of black soldier flies larva accounts for containing thick protein 42～44%, fat 33～35%, crude fibre 7.0%, ash and moisture
16%；Fresh worm is containing protein 17%, fat 12%.Black soldier flies amino acid content is the highest, similar to herring meal, it is necessary to aminoacid
Accounting for more than the 50% of total amino acid content, it is necessary to total amino acid content is 2～3 times of fish flour, methionine content is 2.7 times of fish flour, relies
The content of propylhomoserin is 2.6 times of fish flour.Therefore, black soldier flies not only can be as the living body biological bait needed for a lot of special cultivations
Material, it is also possible to Peru Fish Dietary is as the animal proteinum raw material of Feed Manufacturing.
Black soldier flies in terms of environmental protection in application quite varied, such as be used for process changing food waste, fowl and animal excrement, die of illness fowl
Poultry etc..Border is surveyed factually, and stratiomyiid larva is the highest to the transformation efficiency of changing food waste, and the changing food waste of 70% water content per ton can be given birth to
Produce the fresh worm of stratiomyiid of about 250kg.According to analysis, the black soldier flies larva dry in 4 ages is up to more than 35%, and wherein protein content reaches
50%, fat content is 20%, and multinomial nutritive index all exceeds bean cake.Stratiomyiid larva can be used directly to poultry, duck, fish, the frog, Testudinis
Deng animal, it is also possible to drying and crushing Peru Fish Dietary, bean cake etc. are as the protein feed source of animal.According to scientific research, at black soldier flies
During reason changing food waste, it is possible to the most corrupt composition in rapid digestion changing food waste, the potent pathogenic bacteria killed in rubbish.It addition, elder brother
The conversion of worm, can block the propagation in food chain of the homologous protein virus completely.
Summary of the invention
The technical problem to be solved: narrow for current black soldier flies larva culture technique, protein content improves
The problem of difficulty, the invention provides the cultural method of a kind of high protein black soldier flies pupa, and the present invention is first by black soldier flies hero pupa and female
Pupa is laid eggs, and carries out cultivating larvae, and cultivate larva first with changing food waste after collecting black soldier flies ovum, cultivation
After be carried out after, place in culture dish, and be sequentially added into the materials such as peptone, discarded dead fish, Semen Maydis powder, control temperature
Cultivating, every day all adds peptone and skim milk, until cultivation is to the prepupa stage, collects the black soldier flies pupa after decortication,
Freezen protective, i.e. can get high protein black soldier flies pupa, and the protein content of the black soldier flies pupa of present invention cultivation reaches more than 65%, battalion
Support abundant, can be with a wide range of applications in terms of beauty treatment, food, and the survival rate of cultural method is high, simple to operate.
For solving above-mentioned technical problem, the technical solution used in the present invention is:
(1) respectively 200～300 black soldier flies hero pupas and 200～300 female pupas of black soldier flies are put in gauze, in temperature simultaneously
It is 25～30 DEG C and 60～70% and carries out emerging adult under humidity, in gauze, put into container after emerging adult, and will container fill
Enter 5～8kg humidity be 60～70% peanut residue and 5～10kg humidity be 30～50% dead dead fish of shrimp, controlling temperature is 20～25 DEG C
It is 150～200 μm ol m with light intensity-2·s-1Under conditions of lay eggs, ovum trapper is arranged on container side and receives
Collection black soldier flies ovum；
(2) the black soldier flies ovum that above-mentioned collection obtains is placed in transparent plastic casing, and is moulding by black soldier flies ovum mass ratio 1:20
The feedstuff of uniform spreading last layer newly hatched larvae in magazine, controls temperature at 23～28 DEG C, and humidity is to carry out under conditions of 85～90%
Hatching, at plastic casing upper berth fly net during hatching, obtains black soldier flies larva after hatching；
(3) by above-mentioned black soldier flies larva by 50～100g/ tons add water content be 70～80% changing food waste in, and in temperature
It is 25～30 DEG C and humidity is to cultivate 3～5 days under conditions of 60～70%, the black soldier flies larva after cultivation is taken out, uses quality
Mark 0.1% potassium permanganate solution cleans 2～3 times with tap water after cleaning 2～5min again；
(4) choose the black soldier flies larva after 400～500 above-mentioned cleanings to put in 200cm × 200cm culture dish, and use nylon
Gauze seals culture dish mouth, be sequentially added in culture dish the discarded dead fish of 500～800g peptones, 1～2kg, 800～
1000g Semen Maydis powder, 1～3kg Testa Tritici, 1～2kg60～70 mesh wheat stalk powder and 3～5L skim milks, after stirring mixing,
Black soldier flies larva is cultivated at a temperature of 25～30 DEG C 5～7 days, in culture dish, just increased by 300～500g eggs subsequently every 1 day
White peptone and 100～200mL skim milks, cultivate and harden to polypide wall, stop taking food, enter the prepupa stage, finally collect decortication
After black soldier flies pupa, and put in refrigerator, preserve under the conditions of-10～-5 DEG C, can high protein black soldier flies pupa.
The feedstuff of described newly hatched larvae is to count by weight, chooses 30～50 parts of Semen sojae atricolor powder, 10～20 portions of fishes respectively
Powder, 5～10 parts of throw-out chopped meat, 20～30 parts of rye grass powder, 10～15 portions of oatmeals and 55～65 parts of distilled water, stirring mixing
Uniformly.
The protein content of black soldier flies pupa of present invention cultivation reaches more than 60%, the survival rate of cultural method more than 92%,
Can be with a wide range of applications in terms of beauty treatment, food.
The present invention is compared with additive method, and Advantageous Effects is:
(1) protein content of the black soldier flies pupa of present invention cultivation reaches more than 60%；
(2) the black soldier flies pupa that present invention cultivation obtains can be with a wide range of applications in terms of beauty treatment, food；
(3) survival rate of the cultural method of the present invention is high, more than 92%, and also simple to operate.
Detailed description of the invention
Respectively 200～300 black soldier flies hero pupas and 200～300 female pupas of black soldier flies are put in gauze, in temperature simultaneously
It is 25～30 DEG C and 60～70% and carries out emerging adult under humidity, in gauze, put into container after emerging adult, and will container fill
Enter 5～8kg humidity be 60～70% peanut residue and 5～10kg humidity be 30～50% dead dead fish of shrimp, controlling temperature is 20～25 DEG C
It is to lay eggs under conditions of 150～200 μm ol m-2 s-1 with light intensity, ovum trapper is arranged on container side and receives
Collection black soldier flies ovum；The black soldier flies ovum above-mentioned collection obtained is placed in transparent plastic casing, and presses black soldier flies ovum mass ratio 1:20
The feedstuff of uniform spreading last layer newly hatched larvae in plastic casing, control temperature is at 23～28 DEG C, under conditions of humidity is 85～90%
Hatch, at plastic casing upper berth fly net during hatching, after hatching, obtain black soldier flies larva；By above-mentioned black soldier flies larva by 50～
100g/ ton add water content be 70～80% changing food waste in, and at the bar that temperature is 25～30 DEG C and humidity is 60～70%
Under part cultivate 3～5 days, will cultivation after black soldier flies larva take out, with mass fraction 0.1% potassium permanganate solution clean 2～
Clean 2～3 times with tap water again after 5min；Take the black soldier flies larva after 400～500 above-mentioned cleanings and put into 200cm × 200cm
In culture dish, and seal culture dish mouth with nylon gauze, in culture dish, be sequentially added into 500～800g peptones, 1～2kg useless
Dead fish, 800～the 1000g Semen Maydis powder, 1～3kg Testa Tritici, 1～the 2kg60 that abandon～70 mesh wheat stalk powder and 3～5L defat cattle
Milk, after stirring mixing, cultivates black soldier flies larva 5～7 days at a temperature of 25～30 DEG C, subsequently every 1 day just in culture dish
Increase by 300～500g peptones and 100～200mL skim milks, cultivate and harden to polypide wall, stop taking food, enter prepupa rank
Section, finally collects the black soldier flies pupa after decortication, and puts in refrigerator, preserve under the conditions of-10～-5 DEG C, can high protein Heisui River
Horsefly pupa.
The feedstuff of described newly hatched larvae is to count by weight, chooses 30～50 parts of Semen sojae atricolor powder, 10～20 portions of fishes respectively
Powder, 5～10 parts of throw-out chopped meat, 20～30 parts of rye grass powder, 10～15 portions of oatmeals and 55～65 parts of distilled water, stirring mixing
Uniformly.
Example 1
Respectively 200 black soldier flies hero pupas and 200 female pupas of black soldier flies are put in gauze simultaneously, be 25 DEG C and 60% wet in temperature
Carry out emerging adult under degree, in gauze, put into container after emerging adult, and be 60% peanut residue by container loads 5kg humidity
Being the 30% dead dead fish of shrimp with 5kg humidity, ovum trapper is arranged on container side to collect black soldier flies ovum, controlling temperature is 20 DEG C
It is 150 μm ol m with light intensity-2·s-1Under conditions of lay eggs；The black soldier flies ovum above-mentioned collection obtained is placed in transparent
In plastic casing, and press black soldier flies ovum mass ratio 1:20 feedstuff of uniform spreading last layer newly hatched larvae in plastic casing, control temperature
At 23 DEG C, humidity is to hatch under conditions of 85%, at plastic casing upper berth fly net during hatching, obtains black soldier flies children after hatching
Worm；Above-mentioned black soldier flies larva is added in the changing food waste that water content is 70% by 50g/ ton, and is 25 DEG C and humidity is in temperature
Cultivate 3 days under conditions of 60%, the black soldier flies larva after cultivation is taken out, cleans with mass fraction 0.1% potassium permanganate solution
Clean 2 times with tap water again after 2min；Take the black soldier flies larva after 400 above-mentioned cleanings and put into 200cm × 200cm culture dish
In, and seal culture dish mouth with nylon gauze, in culture dish, it is sequentially added into 500g peptone, dead fish discarded for 1kg, 800g jade
Rice flour, 1kg Testa Tritici, 1kg60 mesh wheat stalk powder and 3L skim milk, after stirring mixing, by black soldier flies larva 25 DEG C of temperature
The lower cultivation of degree 5 days, just increased 300g peptone and 100mL skim milk subsequently in culture dish every 1 day, and cultivation is to polypide wall
Hardening, stops taking food, and enters the prepupa stage, finally collects the black soldier flies pupa after decortication, and put in refrigerator ,-10 DEG C of conditions
Lower preservation, can high protein black soldier flies pupa.
The feedstuff of described newly hatched larvae is to count by weight, choose respectively 30 parts of Semen sojae atricolor powder, 10 parts of fish flour, 5 parts thin
Carnis Sus domestica foam, 20 parts of rye grass powder, 10 portions of oatmeals and 55 parts of distilled water, be uniformly mixed.
The protein content of the black soldier flies pupa of present invention cultivation reaches 62%, and the survival rate of cultural method, can be in U.S. 93%
Hold, food aspect is with a wide range of applications.
Example 2
Respectively 250 black soldier flies hero pupas and 250 female pupas of black soldier flies are put in gauze simultaneously, be 27 DEG C and 65% wet in temperature
Carry out emerging adult under degree, in gauze, put into container after emerging adult, and be 65% peanut residue by container loads 6kg humidity
Being the 40% dead dead fish of shrimp with 7kg humidity, ovum trapper is arranged on container side to collect black soldier flies ovum, controlling temperature is 23 DEG C
It is 175 μm ol m with light intensity-2·s-1Under conditions of lay eggs；The black soldier flies ovum above-mentioned collection obtained is placed in transparent
In plastic casing, and press black soldier flies ovum mass ratio 1:20 feedstuff of uniform spreading last layer newly hatched larvae in plastic casing, control temperature
At 25 DEG C, humidity is to hatch under conditions of 88%, at plastic casing upper berth fly net during hatching, obtains black soldier flies children after hatching
Worm；Above-mentioned black soldier flies larva is added in the changing food waste that water content is 75% by 75g/ ton, and is 27 DEG C and humidity is in temperature
Cultivate 4 days under conditions of 65%, the black soldier flies larva after cultivation is taken out, cleans with mass fraction 0.1% potassium permanganate solution
Clean 2 times with tap water again after 3min；Take the black soldier flies larva after 450 above-mentioned cleanings and put into 200cm × 200cm culture dish
In, and seal culture dish mouth with nylon gauze, in culture dish, it is sequentially added into 650g peptone, dead fish discarded for 1.5kg, 900g
Semen Maydis powder, 2kg Testa Tritici, 1.5kg65 mesh wheat stalk powder and 4L skim milk, after stirring mixing, by black soldier flies larva 27
Cultivating at a temperature of DEG C 6 days, just increased 400g peptone and 150mL skim milk subsequently every 1 day in culture dish, cultivation is to worm
Body wall hardens, and stops taking food, and enters the prepupa stage, finally collects the black soldier flies pupa after decortication, and put in refrigerator, at-7 DEG C of bars
Preserve under part, can high protein black soldier flies pupa.
The feedstuff of described newly hatched larvae is to count by weight, choose respectively 40 parts of Semen sojae atricolor powder, 15 parts of fish flour, 8 parts thin
Carnis Sus domestica foam, 25 parts of rye grass powder, 13 portions of oatmeals and 60 parts of distilled water, be uniformly mixed.
The protein content of the black soldier flies pupa of present invention cultivation reaches 63%, and the survival rate of cultural method, can be in U.S. 95%
Hold, food aspect is with a wide range of applications.
Example 3
Respectively 300 black soldier flies hero pupas and 300 female pupas of black soldier flies are put in gauze simultaneously, be 30 DEG C and 70% wet in temperature
Carry out emerging adult under degree, in gauze, put into container after emerging adult, and be 70% peanut residue by container loads 8kg humidity
Being the 50% dead dead fish of shrimp with 10kg humidity, ovum trapper is arranged on container side to collect black soldier flies ovum, controlling temperature is 25 DEG C
It is 200 μm ol m with light intensity-2·s-1Under conditions of lay eggs；The black soldier flies ovum above-mentioned collection obtained is placed in transparent
In plastic casing, and press black soldier flies ovum mass ratio 1:20 feedstuff of uniform spreading last layer newly hatched larvae in plastic casing, control temperature
At 28 DEG C, humidity is to hatch under conditions of 90%, at plastic casing upper berth fly net during hatching, obtains black soldier flies children after hatching
Worm；Above-mentioned black soldier flies larva is added in the changing food waste that water content is 80% by 100g/ ton, and is 30 DEG C and humidity in temperature
It is to cultivate 5 days under conditions of 70%, the black soldier flies larva after cultivation is taken out, clear with mass fraction 0.1% potassium permanganate solution
Clean 3 times with tap water again after washing 5min；Take the black soldier flies larva after 500 above-mentioned cleanings and put into 200cm × 200cm culture dish
In, and seal culture dish mouth with nylon gauze, in culture dish, it is sequentially added into 800g peptone, dead fish discarded for 2kg, 1000g
Semen Maydis powder, 3kg Testa Tritici, 2kg70 mesh wheat stalk powder and 5L skim milk, after stirring mixing, by black soldier flies larva at 30 DEG C
At a temperature of cultivate 7 days, in culture dish, just increased 500g peptone and 200mL skim milk subsequently every 1 day, cultivation is to polypide
Wall hardens, and stops taking food, and enters the prepupa stage, finally collects the black soldier flies pupa after decortication, and put in refrigerator ,-5 DEG C of conditions
Lower preservation, can high protein black soldier flies pupa.
The feedstuff of described newly hatched larvae is to count by weight, choose respectively 50 parts of Semen sojae atricolor powder, 20 parts of fish flour, 10 parts thin
Carnis Sus domestica foam, 30 parts of rye grass powder, 15 portions of oatmeals and 65 parts of distilled water, be uniformly mixed.
The protein content of the black soldier flies pupa of present invention cultivation reaches 65%, and the survival rate of cultural method, can be in U.S. 96%
Hold, food aspect is with a wide range of applications.
Claims (2)
1. the cultural method of a high protein black soldier flies pupa, it is characterised in that concrete cultivation step is:
(1) respectively 200～300 black soldier flies hero pupas and 200～300 female pupas of black soldier flies are put in gauze, in temperature simultaneously
It is 25～30 DEG C and 60～70% and carries out emerging adult under humidity, in gauze, put into container after emerging adult, and will container fill
Enter 5～8kg humidity be 60～70% peanut residue and 5～10kg humidity be 30～50% dead dead fish of shrimp, controlling temperature is 20～25 DEG C
It is 150～200 μm ol m with light intensity-2·s-1Under conditions of lay eggs, ovum trapper is arranged on container side and receives
Collection black soldier flies ovum；
(2) the black soldier flies ovum that above-mentioned collection obtains is placed in transparent plastic casing, and is moulding by black soldier flies ovum mass ratio 1:20
The feedstuff of uniform spreading last layer newly hatched larvae in magazine, controls temperature at 23～28 DEG C, and humidity is to carry out under conditions of 85～90%
Hatching, at plastic casing upper berth fly net during hatching, obtains black soldier flies larva after hatching；
(3) by above-mentioned black soldier flies larva by 50～100g/ tons add water content be 70～80% changing food waste in, and in temperature
It is 25～30 DEG C and humidity is to cultivate 3～5 days under conditions of 60～70%, the black soldier flies larva after cultivation is taken out, uses quality
Mark 0.1% potassium permanganate solution cleans 2～3 times with tap water after cleaning 2～5min again；
(4) choose the black soldier flies larva after 400～500 above-mentioned cleanings to put in 200cm × 200cm culture dish, and use nylon
Gauze seals culture dish mouth, be sequentially added in culture dish the discarded dead fish of 500～800g peptones, 1～2kg, 800～
1000g Semen Maydis powder, 1～3kg Testa Tritici, 1～2kg60～70 mesh wheat stalk powder and 3～5L skim milks, after stirring mixing,
Black soldier flies larva is cultivated at a temperature of 25～30 DEG C 5～7 days, in culture dish, just increased by 300～500g eggs subsequently every 1 day
White peptone and 100～200mL skim milks, cultivate and harden to polypide wall, stop taking food, enter the prepupa stage, finally collect decortication
After black soldier flies pupa, and put in refrigerator, preserve under the conditions of-10～-5 DEG C, can high protein black soldier flies pupa.
The cultural method of a kind of high protein black soldier flies pupa the most according to claim 1, it is characterised in that: described first incubate children
The feedstuff of worm is to count by weight, choose respectively 30～50 parts of Semen sojae atricolor powder, 10～20 parts of fish flour, 5～10 parts of throw-out chopped meat, 20
～30 parts of rye grass powder, 10～15 portions of oatmeals and 55～65 parts of distilled water, it is uniformly mixed.
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Cited By (14)
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| CN107347811A (en) * | 2017-07-06 | 2017-11-17 | 江苏农牧科技职业学院 | A kind of indoor culture method of black soldier flies |
| RU2651303C1 (en) * | 2017-08-23 | 2018-04-19 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Российский экономический университет имени Г.В. Плеханова" | Method for obtaining the biologically active fodder additive for chicken-broilers |
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| CN108338129A (en) * | 2018-02-10 | 2018-07-31 | 华中农业大学 | A method of promote the flat angle stratiomyiid of speck to pupate and sprout wings |
| CN108669383A (en) * | 2018-03-28 | 2018-10-19 | 广州飞禧特生物科技有限公司 | A kind of black soldier flies feed and preparation method thereof |
| CN108925755A (en) * | 2018-03-26 | 2018-12-04 | 四川芬尼格斯科技有限公司 | A kind of breeding bait formula of black soldier flies larva and preparation method thereof |
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| CN110999875A (en) * | 2020-01-08 | 2020-04-14 | 贵州天迪绿水青山生物科技有限责任公司 | Method for breeding hermetia illucens by using biological garbage |
| CN111742889A (en) * | 2020-06-24 | 2020-10-09 | 汕头安芮洁环保科技有限公司 | Breeding method of hermetia illucens |
| CN111972570A (en) * | 2020-08-24 | 2020-11-24 | 普罗欧生物科技(武汉)有限公司 | Feed for improving protein content and activity of hermetia illucens and preparation method thereof |
| CN113925023A (en) * | 2021-10-18 | 2022-01-14 | 江苏函夏新农业科技有限公司 | Method for breeding black soldier fly commercial larvae by mixing chicken manure and lysimachia foenum-graecum as feed |
| CN115176766A (en) * | 2022-08-09 | 2022-10-14 | 深圳市中兴恒熙环保有限公司 | Method for feeding hermetia illucens larvae by using food waste and application |
| CN115943931A (en) * | 2022-12-07 | 2023-04-11 | 江苏省农业科学院 | Ammonia gas emission reduction method in black soldier fly bioconversion process |
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| CN111742889A (en) * | 2020-06-24 | 2020-10-09 | 汕头安芮洁环保科技有限公司 | Breeding method of hermetia illucens |
| CN111972570A (en) * | 2020-08-24 | 2020-11-24 | 普罗欧生物科技(武汉)有限公司 | Feed for improving protein content and activity of hermetia illucens and preparation method thereof |
| CN113925023A (en) * | 2021-10-18 | 2022-01-14 | 江苏函夏新农业科技有限公司 | Method for breeding black soldier fly commercial larvae by mixing chicken manure and lysimachia foenum-graecum as feed |
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