CN113925023A - Method for breeding black soldier fly commercial larvae by mixing chicken manure and lysimachia foenum-graecum as feed - Google Patents
Method for breeding black soldier fly commercial larvae by mixing chicken manure and lysimachia foenum-graecum as feed Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a method for breeding black soldier fly commercial larvae by mixing chicken manure and lysimachia foenum-graecum as feed, which comprises the following steps: carrying out dehydration treatment on the pure chicken manure; adding the chicken manure into rice bran or rice fine shell, adding a small amount of lactic acid bacteria and a manure feed leavening agent, and carrying out anaerobic fermentation on the chicken manure; hatching the hermetia illucens eggs to hatch early-stage hermetia illucens larvae; feeding the early-stage black soldier fly larvae, and supplementing chicken manure and fresh grass pulp of the leaf-eating grass in real time in the feeding process; after the culture maturity time is reached, the mature hermetia illucens commercial larvae are separated out. The method for breeding the commercial black soldier fly larvae enables the breeding environment temperature of the black soldier fly larvae to be controlled between 30 ℃ and 40 ℃ after 3 days, the black soldier fly larvae cannot be damaged, the defect of nutritional ingredients of the black soldier fly larvae bred by pure chicken manure is overcome, the organic matter content of excrement of the black soldier fly larvae reaches 93.6%, and the fertility index of the black soldier fly larvae is higher than the national ingredient standard for organic fertilizers.
Description
Technical Field
The invention relates to a method for breeding black soldier fly commercial larvae, in particular to a method for breeding black soldier fly commercial larvae by mixing chicken manure and herbivore as feed.
Background
At present, the black soldier fly larvae are raised by taking pure chicken manure as feed at home and abroad, but the existing raising technology has the following defects and shortcomings:
(1) after the black soldier fly larvae grow to 3 days, the pure chicken manure feed generates a high temperature higher than 50 ℃, so that the phenomenon that the black soldier fly larvae die due to overhigh temperature is easily caused, and the growth of the larvae is seriously influenced;
(2) the nutrition ingredients of the black soldier fly larvae fed by the pure chicken manure feed are insufficient, and the contents of protein, various amino acids, crude fiber and fat in the chicken manure are mainly insufficient, so that the growth and development of the black soldier fly larvae are greatly influenced;
(3) the content of nitrogen, phosphorus and potassium in the excrement pulled out by the larvae of the hermetia illucens larvae is not high, and if the excrement of the hermetia illucens larvae is used for preparing an organic fertilizer, the fertilizer efficiency is not high.
Disclosure of Invention
The purpose of the invention is as follows: the method for breeding the hermetia illucens commercial larvae by mixing the chicken manure and the lysimachia foenum-graecum as the feed has the advantages of high survival rate, high growth efficiency and high manure recycling value.
The technical scheme is as follows: the invention relates to a method for feeding hermetia illucens commercial larvae by mixing chicken manure and herbivorous herbs as feeds, which comprises the following steps:
step 1, carrying out dehydration treatment on pure chicken manure;
step 2, adding rice bran or rice fine shells into the chicken manure after dehydration treatment, and then adding a small amount of lactic acid bacteria and a manure feed leavening agent to perform anaerobic fermentation on the chicken manure;
step 3, hatching the hermetia illucens eggs, and hatching early-stage hermetia illucens larvae;
step 4, placing the anaerobically fermented chicken manure into a culture pond, adding the fresh grass pulp of the leaf-eating grass into the culture pond, placing the hatched small black soldier fly larvae in the initial stage into the culture pond for breeding, and supplementing the chicken manure and the fresh grass pulp of the leaf-eating grass into the culture pond in real time in the breeding process;
and 5, after the culture maturity time is reached, carrying out worm and sand separation, and separating out mature hermetia illucens commercial larvae.
Further, in the step 1, when the pure chicken manure is dehydrated, a dry-wet separator is used for dehydrating, and the water content of the chicken manure is controlled between 65% and 70%.
Further, in the step 2, when the chicken manure is added into the rice bran or the rice fine shell, the chicken manure accounts for 8-12% of the total weight.
Further, in step 2, during anaerobic fermentation, if the current time is summer, the fermentation time is 2-4 days, and if the current time is spring, autumn or winter, the fermentation time is 4-7 days.
Further, in the step 3, when the hermetia illucens eggs are incubated, the hermetia illucens eggs are placed in the incubation plastic boxes for incubation, and the egg placing amount in each incubation plastic box is 250-260 g/m2。
Further, in the step 3, when the hermetia illucens eggs are hatched, the bran serving as the base material and the fresh grass pulp serving as the leaf-eating grass are mixed and then laid at the bottom of the hatching plastic box as the hatching base material, and the laying thickness of the hatching base material is 4-6 cm.
Furthermore, the mass ratio of the bran as the base material to the fresh grass pulp of the leafy grass in the hatching base material is 3:2, and the moisture of the hatching base material is 65-75 percent.
Further, in the step 3, when the hermetia illucens eggs are incubated, the incubation temperature is set to be 28-32 ℃, and the whole incubation period is 3.8-4.2 days.
Further, in the step 4, when the chicken manure and the fresh grass pulp of the leafy grass which are subjected to anaerobic fermentation are added into the culture pond, the chicken manure and the fresh grass pulp of the leafy grass are added according to the mass ratio of 4: 1.
Further, in step 5, the cultivation maturation time is as follows: the duration of the method is 5-7 days in summer, and the duration of the method is 7-10 days in spring, autumn and winter.
Compared with the prior art, the invention has the beneficial effects that: (1) the method can solve the problem that the internal temperature of the breeding environment of the hermetia illucens larvae is controlled to be between 30 and 40 ℃ after 3 days, and the hermetia illucens larvae cannot be damaged by high temperature; (2) the method can completely solve the nutritional ingredient defect of the black soldier fly larvae bred by pure chicken manure, the protein content in the chicken manure is not high, generally about 25%, about 8.5% of various amino acids, about 1.76% of crude fat, about 13% of crude fiber, about 3% of the sum of three types of nitrogen, phosphorus and potassium, the dry matter in the leaf eating grass contains 48.7% of protein, about 7.8% of fat, about 26.4% of total dietary fiber, about 35.4% of various amino acids, about 6% of the sum of three types of nitrogen, phosphorus and potassium, and the leaf eating grass contains abundant beneficial minerals, SOD, isoflavonides and the like, so the quality and the yield of the black soldier fly larvae are greatly improved by adding the fresh grass pulp of the leaf eating grass into the chicken manure according to the method; (3) the organic matters of the excrement pulled out by the hermetia illucens larvae bred by the method reach 93.6 percent, the nitrogen reaches 3.08 percent, the phosphorus reaches 2.92 percent and the potassium reaches 3.03 percent, and the fertility index of the excrement is higher than the national organic fertilizer ingredient standard.
Drawings
FIG. 1 is a flow chart of the method of the present invention.
Detailed Description
The technical solution of the present invention is described in detail below with reference to the accompanying drawings, but the scope of the present invention is not limited to the embodiments.
The black soldier fly bred by the method is also called hermetia illucens of hermetia illucens, and is an insect of hermetia illucens of hermetiaceae of diptera. The life cycle of the hermetia illucens is in the development stage of 4 insect states of eggs, larvae, pupae and adults, and the time from the eggs to the adults is about 35 days. The hermetia illucens larvae contain animal protein and high fat, can be used as a large amount of feed for aquatic land-born poultry, completely replaces fish meal, and contains a large amount of antibacterial peptide, so that the fed animals are not easy to get ill, and the disease resistance rate is improved by over 95 percent.
The content of the nutrient components of the chicken manure which is one of the feeding raw materials of the invention is as follows:
one of the breeding raw materials of the invention is the leaf-eating grass which is also called proteinic grass and amino acid grass. Perennial root herbaceous plants, Polygonaceae Rumex genus. The proteinic grass (proteinic grass) is a nutrient plant with high protein, high nutrition, high yield and high benefit, has comprehensive values of nutrition, edibility, applicability, adaptability, yield performance and the like, and can be developed and developed in diversified fields of grain, food, health care, medicine, cultivation, feed, fertilizer, soil improvement, environmental protection and the like. The leaf-eating grass (proteinic grass) contains 36 to 48.7 percent of protein, 35.40 percent of total amount of eighteen amino acids, 40.0mg/100g of vitamin E and 47.3mg/100g of ascorbic acid; is rich in beta-carotene, chlorophyll, emodin, isoflavone, SOD, selenium, potassium, calcium, iron, zinc, phosphorus, magnesium and other trace elements and beneficial mineral substances, has the nutrient elements required by human bodies, and has the highest content and the most complete elements. Therefore, the new location of the leaf eating grass (the proteinic grass) — the 'plant gold mine' and the 'plant diamond'.
The embodiment of the invention comprises the following steps:
as shown in figure 1, the method for feeding the commercial black soldier fly larvae by mixing the chicken manure and the leaf-eating grass as the feed comprises the following steps:
step 1, carrying out dehydration treatment on pure chicken manure;
step 2, adding the dehydrated chicken manure into rice bran or rice fine shells, further enhancing the bulkiness and air permeability of the chicken manure by using the rice bran or the rice fine shells, and adding a small amount of lactic acid bacteria and a manure feed leavening agent to perform anaerobic fermentation on the chicken manure;
step 3, hatching the hermetia illucens eggs in the chicken manure fermentation process, and hatching early-stage hermetia illucens larvae;
step 4, placing the anaerobically fermented chicken manure into a culture pond, adding the fresh grass pulp of the phylloxera micranthus into the culture pond, placing the hatched small larvae of the hermetia illucens at the initial stage into the culture pond for breeding, and supplementing the chicken manure and the fresh grass pulp of the phylloxera micranthus in real time into the culture pond in the breeding process, wherein the aim of adding the fresh grass pulp of the phylloxera micranthus is to effectively reduce the high temperature generated when the pure chicken manure is used for breeding the hermetia illucens larvae, effectively adjust the dryness and humidity of the breeding environment, and effectively supplement the nutrient components such as plant protein, amino acid, fat and the like for breeding the hermetia illucens larvae;
and 5, after the cultivation maturity time is reached, carrying out worm and sand separation, and separating out mature hermetia illucens commercial larvae, wherein the commercial larvae can be directly feeds for aquatic products and land-grown poultry, and can be used for drying animal protein sources for various feeds.
Further, in the step 1, when the pure chicken manure is dehydrated, a dry-wet separator is used for dehydrating, and the water content of the chicken manure is controlled between 65% and 70%. The dehydration treatment by using the dry-wet separator can control the dehydration water content, adjust the structure of an oil film on the surface layer of the chicken manure, enhance the bulkiness and the air permeability of the pure chicken manure, accelerate the feeding of the black soldier fly larvae in the early stage and build a good culture environment;
further, in step 2, when the chicken manure is added into the rice bran or the rice fine shell, the chicken manure accounts for 8-12% of the total weight, and preferably 10%.
Further, in step 2, during the anaerobic fermentation, if the current time is summer, the fermentation time is 2-4 days, preferably 3 days, and if the current time is spring, autumn or winter, the fermentation time is 4-7 days.
Further, in the step 3, when the hermetia illucens eggs are incubated, the hermetia illucens eggs are placed in the incubation plastic boxes for incubation, and the egg placing amount in each incubation plastic box is 250-260 g/m2Preferably, 50 g of ova are put into a hatching plastic box of 40cm multiplied by 50 cm.
Further, in the step 3, when the hermetia illucens eggs are hatched, bran serving as a base material and fresh grass pulp serving as a leaf-eating grass are mixed and then laid at the bottom of the hatching plastic box as a hatching base material, and the laying thickness of the hatching base material is 4-6 cm, preferably 5 cm.
Furthermore, the mass ratio of the bran as the base material to the fresh grass pulp of the leafy grass in the hatching base material is 3:2, and the moisture of the hatching base material is 65-75%, preferably 70%.
Further, in the step 3, when the hermetia illucens eggs are incubated, the incubation temperature is set to be 28-32 ℃, and the whole incubation period is 3.8-4.2 days, preferably 4 days.
Further, in the step 4, when the chicken manure and the fresh grass pulp of the leafy grass which are subjected to anaerobic fermentation are added into the culture pond, the chicken manure and the fresh grass pulp of the leafy grass are added according to the mass ratio of 4: 1.
Further, in step 5, the cultivation maturation time is as follows: the duration of the summer is 5-7 days, preferably 6 days, and the duration of the spring, the autumn and the winter is 7-10 days.
The method for feeding the commercial black soldier fly larvae by mixing the chicken manure and the lysimachia foenum-graecum as the feed has the advantages that:
(1) the method can solve the problem that the internal temperature of the breeding environment of the hermetia illucens larvae is controlled to be between 30 and 40 ℃ after 3 days, and the hermetia illucens larvae cannot be damaged by high temperature;
(2) the method can completely solve the nutritional ingredient defect of the black soldier fly larvae bred by pure chicken manure, the protein content in the chicken manure is not high, generally about 25%, about 8.5% of various amino acids, about 1.76% of crude fat, about 13% of crude fiber, about 3% of the sum of three types of nitrogen, phosphorus and potassium, the dry matter in the leaf eating grass contains 48.7% of protein, about 7.8% of fat, about 26.4% of total dietary fiber, about 35.4% of various amino acids, about 6% of the sum of three types of nitrogen, phosphorus and potassium, and the leaf eating grass contains abundant beneficial minerals, SOD, isoflavonides and the like, so the quality and the yield of the black soldier fly larvae are greatly improved by adding the fresh grass pulp of the leaf eating grass into the chicken manure according to the method;
(3) the organic matters of the excrement pulled out by the hermetia illucens larvae bred by the method reach 93.6 percent, the nitrogen reaches 3.08 percent, the phosphorus reaches 2.92 percent and the potassium reaches 3.03 percent, and the fertility index of the excrement is higher than the national organic fertilizer ingredient standard.
As noted above, while the present invention has been shown and described with reference to certain preferred embodiments, it is not to be construed as limited thereto. Various changes in form and detail may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (10)
1. A method for breeding hermetia illucens commercial larvae by mixing chicken manure and herbivore as feeds is characterized by comprising the following steps:
step 1, carrying out dehydration treatment on pure chicken manure;
step 2, adding rice bran or rice fine shells into the chicken manure after dehydration treatment, and then adding a small amount of lactic acid bacteria and a manure feed leavening agent to perform anaerobic fermentation on the chicken manure;
step 3, hatching the hermetia illucens eggs, and hatching early-stage hermetia illucens larvae;
step 4, placing the anaerobically fermented chicken manure into a culture pond, adding the fresh grass pulp of the leaf-eating grass into the culture pond, placing the hatched small black soldier fly larvae in the initial stage into the culture pond for breeding, and supplementing the chicken manure and the fresh grass pulp of the leaf-eating grass into the culture pond in real time in the breeding process;
and 5, after the culture maturity time is reached, carrying out worm and sand separation, and separating out mature hermetia illucens commercial larvae.
2. The method for rearing black soldier fly larvae with feed comprising chicken manure and lysimachia foenum-graecum according to claim 1, wherein in the step 1, the pure chicken manure is dehydrated by a dry-wet separator, and the water content of the chicken manure is controlled to be 65-70%.
3. The method for rearing black soldier fly larvae with chicken manure mixed with leaf-eating grass as feed according to claim 1, wherein in the step 2, the chicken manure is added to the rice bran or the rice chaff so that the chicken manure accounts for 8-12% of the total weight.
4. The method for rearing black soldier fly larvae with feed comprising chicken manure and lysimachia foenum-graecum as claimed in claim 1, wherein in step 2, when anaerobic fermentation is performed, the fermentation time is 2-4 days in summer and 4-7 days in spring, autumn or winter.
5. The method for rearing black soldier fly commercial larvae with chicken manure mixed with lysimachia foenum-graecum as feed according to claim 1, wherein in the step 3, when the eggs of black soldier fly are hatched, the eggs of black soldier fly are placed in hatching plastic boxes for hatching, and the egg laying amount in each hatching plastic box is 250-260 g/m2。
6. The method for feeding hermetia illucens commercial larvae with chicken manure mixed with phylloxera micrantha as feed according to claim 1, wherein in the step 3, when hermetia illucens eggs are hatched, the mixture of bran serving as a base material and fresh grass pulp serving as a phylloxera micrantha is laid at the bottom of a hatching plastic box as a hatching base material, and the laying thickness of the hatching base material is 4-6 cm.
7. The method for rearing black soldier fly larvae with chicken manure mixed with herbivore as feed according to claim 6, wherein the mass ratio of the bran as the base material to the pulp of the fresh herbivore in the hatching base material is 3:2, and the moisture content of the hatching base material is 65-75%.
8. The method for rearing black soldier fly larvae with chicken manure mixed with lysimachia foenum-graecum as feed according to claim 1, wherein in the step 3, when eggs of black soldier fly are hatched, the hatching temperature is set to be between 28 and 32 ℃, and the whole hatching period is 3.8 to 4.2 days.
9. The method for rearing black soldier fly commercial larvae with feed prepared by mixing chicken manure and lysimachia foenum-graecum as claimed in claim 1, wherein in the step 4, when the anaerobically fermented chicken manure and the fresh lysimachia foenum-graecum grass pulp are added into the rearing pond, the ratio of the mass ratio of the chicken manure to the mass ratio of the fresh lysimachia foenum-graecum grass pulp is 4: 1.
10. The method for rearing hermetia illucens commercial larvae with chicken manure mixed with lysimachia foenum-graecum as feed according to claim 1, wherein in the step 5, the rearing maturity time is as follows: the duration of the method is 5-7 days in summer, and the duration of the method is 7-10 days in spring, autumn and winter.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114538969A (en) * | 2022-02-22 | 2022-05-27 | 江苏函夏新农业科技有限公司 | Organic fertilizer production method using chicken manure for breeding hermetia illucens as raw material |
CN115316570A (en) * | 2022-08-11 | 2022-11-11 | 四川农业大学 | Biological feed for breeding hermetia illucens and preparation method thereof |
WO2024072318A1 (en) * | 2022-09-29 | 2024-04-04 | Nutrition Technologies Research Pte. Ltd. | Fermentation methods and systems of making insect feed |
Citations (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104817346A (en) * | 2015-05-19 | 2015-08-05 | 刘金花 | Method for treating excrement by using hermetia illucens and organic fertilizer prepared by method |
CN104824457A (en) * | 2015-05-19 | 2015-08-12 | 刘金花 | Treatment method of excrements for feeding larvae |
CN105433066A (en) * | 2015-12-25 | 2016-03-30 | 广东省农业科学院蚕业与农产品加工研究所 | Method for producing insect protein feed by treating excrements of livestock by virtue of Hermetia illucens |
CN105859332A (en) * | 2016-04-01 | 2016-08-17 | 刘德江 | Excrement biological treatment method for livestock and poultry breeding farm |
CN105906390A (en) * | 2016-07-04 | 2016-08-31 | 湖南楚贤环保科技有限责任公司 | Method for treating kitchen waste |
CN106070067A (en) * | 2016-06-08 | 2016-11-09 | 天津农垦渤海农业集团有限公司 | The method utilizing black soldier flies and white star brevitarsis complete converting animal-poultry faeces step by step |
CN106305631A (en) * | 2016-08-25 | 2017-01-11 | 仇颖超 | Breeding method of high-protein Hermetia illucens pupae |
CN107912613A (en) * | 2017-11-29 | 2018-04-17 | 柏绿山 | With the feed and its production method of food leaf grass production |
CN108450710A (en) * | 2018-03-20 | 2018-08-28 | 广州壹号食品有限责任公司 | A kind of chicken manure concocting method suitable for black soldier flies cultivation |
CN108902448A (en) * | 2018-06-25 | 2018-11-30 | 鲁东大学 | A kind of method of feces of livestock and poultry deodorization |
CN109007284A (en) * | 2018-08-09 | 2018-12-18 | 湖南百宜牧业科技有限公司 | A method of protein feed is prepared using black soldier flies processing pig manure |
CN110078541A (en) * | 2019-05-10 | 2019-08-02 | 云南农业大学 | Black soldier flies are improved to the method for cow dung treatment effeciency using peanut powder |
CN110140694A (en) * | 2019-05-23 | 2019-08-20 | 广东省农业科学院蚕业与农产品加工研究所 | A method of protein feed is produced using pig manure cultivation black soldier flies |
CN111084160A (en) * | 2020-01-22 | 2020-05-01 | 渑池县迈安达农业科技有限公司 | Feeding method for improving black soldier fly yield by utilizing kitchen waste and chicken manure |
CN111406714A (en) * | 2020-05-08 | 2020-07-14 | 华南农业大学 | Treatment method of fresh chicken manure |
-
2021
- 2021-10-18 CN CN202111208836.7A patent/CN113925023A/en active Pending
Patent Citations (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104817346A (en) * | 2015-05-19 | 2015-08-05 | 刘金花 | Method for treating excrement by using hermetia illucens and organic fertilizer prepared by method |
CN104824457A (en) * | 2015-05-19 | 2015-08-12 | 刘金花 | Treatment method of excrements for feeding larvae |
CN105433066A (en) * | 2015-12-25 | 2016-03-30 | 广东省农业科学院蚕业与农产品加工研究所 | Method for producing insect protein feed by treating excrements of livestock by virtue of Hermetia illucens |
CN105859332A (en) * | 2016-04-01 | 2016-08-17 | 刘德江 | Excrement biological treatment method for livestock and poultry breeding farm |
CN106070067A (en) * | 2016-06-08 | 2016-11-09 | 天津农垦渤海农业集团有限公司 | The method utilizing black soldier flies and white star brevitarsis complete converting animal-poultry faeces step by step |
CN105906390A (en) * | 2016-07-04 | 2016-08-31 | 湖南楚贤环保科技有限责任公司 | Method for treating kitchen waste |
CN106305631A (en) * | 2016-08-25 | 2017-01-11 | 仇颖超 | Breeding method of high-protein Hermetia illucens pupae |
CN107912613A (en) * | 2017-11-29 | 2018-04-17 | 柏绿山 | With the feed and its production method of food leaf grass production |
CN108450710A (en) * | 2018-03-20 | 2018-08-28 | 广州壹号食品有限责任公司 | A kind of chicken manure concocting method suitable for black soldier flies cultivation |
CN108902448A (en) * | 2018-06-25 | 2018-11-30 | 鲁东大学 | A kind of method of feces of livestock and poultry deodorization |
CN109007284A (en) * | 2018-08-09 | 2018-12-18 | 湖南百宜牧业科技有限公司 | A method of protein feed is prepared using black soldier flies processing pig manure |
CN110078541A (en) * | 2019-05-10 | 2019-08-02 | 云南农业大学 | Black soldier flies are improved to the method for cow dung treatment effeciency using peanut powder |
CN110140694A (en) * | 2019-05-23 | 2019-08-20 | 广东省农业科学院蚕业与农产品加工研究所 | A method of protein feed is produced using pig manure cultivation black soldier flies |
CN111084160A (en) * | 2020-01-22 | 2020-05-01 | 渑池县迈安达农业科技有限公司 | Feeding method for improving black soldier fly yield by utilizing kitchen waste and chicken manure |
CN111406714A (en) * | 2020-05-08 | 2020-07-14 | 华南农业大学 | Treatment method of fresh chicken manure |
Non-Patent Citations (3)
Title |
---|
柏绿山 等: ""食叶草氨基酸蛋白肥料的应用与效果分析"", 《中国果菜》 * |
柏绿山 等: ""食叶草的研究与开发"", 《中国果菜》 * |
王子维 等: ""鸡粪饲养黑水虻的密度初探"", 《山东畜牧兽医》 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114538969A (en) * | 2022-02-22 | 2022-05-27 | 江苏函夏新农业科技有限公司 | Organic fertilizer production method using chicken manure for breeding hermetia illucens as raw material |
CN115316570A (en) * | 2022-08-11 | 2022-11-11 | 四川农业大学 | Biological feed for breeding hermetia illucens and preparation method thereof |
CN115316570B (en) * | 2022-08-11 | 2023-09-01 | 四川农业大学 | Biological feed for culturing hermetia illucens and preparation method thereof |
WO2024072318A1 (en) * | 2022-09-29 | 2024-04-04 | Nutrition Technologies Research Pte. Ltd. | Fermentation methods and systems of making insect feed |
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