CN111742889A - Breeding method of hermetia illucens - Google Patents
Breeding method of hermetia illucens Download PDFInfo
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- CN111742889A CN111742889A CN202010595543.8A CN202010595543A CN111742889A CN 111742889 A CN111742889 A CN 111742889A CN 202010595543 A CN202010595543 A CN 202010595543A CN 111742889 A CN111742889 A CN 111742889A
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- 238000009395 breeding Methods 0.000 title claims abstract description 71
- 241000709785 Hermetia illucens Species 0.000 title claims abstract description 51
- 235000013601 eggs Nutrition 0.000 claims abstract description 67
- 230000001488 breeding effect Effects 0.000 claims abstract description 40
- 238000000034 method Methods 0.000 claims abstract description 30
- 230000012447 hatching Effects 0.000 claims abstract description 16
- 230000008569 process Effects 0.000 claims abstract description 11
- 230000000694 effects Effects 0.000 claims abstract description 4
- 241000238631 Hexapoda Species 0.000 claims description 36
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 244000063299 Bacillus subtilis Species 0.000 claims description 16
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 16
- 230000032669 eclosion Effects 0.000 claims description 16
- 238000012216 screening Methods 0.000 claims description 15
- 239000000758 substrate Substances 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 7
- 230000003203 everyday effect Effects 0.000 claims description 6
- 230000000968 intestinal effect Effects 0.000 claims description 5
- 210000004681 ovum Anatomy 0.000 claims description 5
- 102000002322 Egg Proteins Human genes 0.000 claims description 4
- 108010000912 Egg Proteins Proteins 0.000 claims description 4
- 241000287828 Gallus gallus Species 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 2
- 238000005057 refrigeration Methods 0.000 claims description 2
- 241000382353 Pupa Species 0.000 abstract description 19
- 238000003306 harvesting Methods 0.000 abstract description 4
- 230000000052 comparative effect Effects 0.000 description 17
- 230000004083 survival effect Effects 0.000 description 15
- 230000017448 oviposition Effects 0.000 description 14
- 230000008901 benefit Effects 0.000 description 4
- 235000013339 cereals Nutrition 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 230000019617 pupation Effects 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 241000209149 Zea Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 235000013312 flour Nutrition 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 239000012254 powdered material Substances 0.000 description 2
- 230000007480 spreading Effects 0.000 description 2
- 241000255925 Diptera Species 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 241001481656 Stratiomyidae Species 0.000 description 1
- 230000036983 biotransformation Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000008144 egg development Effects 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000010815 organic waste Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
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- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a breeding method of hermetia illucens, and belongs to the technical field of hermetia illucens breeding. According to the invention, the eggs in the gastral period are selected for harvesting and hatching, so that the uniformity and activity of the larvae which are hatched initially are ensured, and the hatching rate of the eggs is over 80%; meanwhile, 5-day-old larva rotary disc brooding with the specification of more than 20mg and regular and active growth and 4-year-old larva with the specification of more than 130mg are screened for pre-pupa cultivation, the specification of the obtained larva is ensured to be more than 200mg, and the specification of pupa is more than 160 mg; the breeding process is standardized, so that the hermetia illucens can be stably bred in each batch.
Description
Technical Field
The invention belongs to the technical field of hermetia illucens breeding, and relates to a breeding method of hermetia illucens.
Background
Hermitia illucens (Hermitia illucens), also known as Hermitia illucens, also known as Black soldier fly, belongs to the Diptera family of Hermitiaceae. With the development of artificial breeding, hermetia illucens are distributed all over the country, wherein the distribution is the most in south China. The black soldier fly has four insect states, namely eggs, larvae, pupae and adults. The larvae have six instars, also called prepupa. The characteristics of nutrition, feeding amount, stress resistance and biotransformation capability of larvae and harmlessness of adults to human beings provide convenience for large-scale artificial breeding of hermetia illucens.
The general steps of the hermetia illucens breeding are as follows: collecting eggs and collecting eggs, enabling the larvae hatched by the eggs to crawl into feed, taking the feed to 3 years old, treating organic waste to prepupa by 3 instar insects, screening the prepupa into adult breeding chambers to pupate, enabling the pupae to eclosion and lay eggs, and collecting the eggs. However, in the prior art, due to the fact that survival rates of larvae are different, breeding is extensive, standardization of breeding cannot be achieved, the seed worm collecting time is long, the size of the obtained seed worm is small, and single-female spawning is low, so that the method for breeding the hermetia illucens with short seed worm collecting time, large size of the obtained seed worm and high single-female spawning is important.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention aims to provide a breeding method of hermetia illucens.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows: the breeding method of the hermetia illucens comprises the following steps:
(1) culturing ovum in 28-30 deg.C environment with humidity of 60-80% to original intestinal stage, and collecting ovum in original intestinal stage;
(2) hatching the eggs in the protogut period in an environment with the temperature of 28-30 ℃ and the humidity of 60-80%, and collecting the primarily hatched larvae;
(3) quantitatively placing the primarily hatched larvae on a culture substrate, culturing in an environment with the temperature of 28-30 ℃ and the humidity of 60-80%, and screening 5-day-old larvae with the specification of more than 20mg, regular growth and activity when the primarily hatched larvae grow to 5 days old to obtain qualified larvae;
(4) quantitatively transferring the qualified larvae to a culture bed to culture the larvae to 4 instar insects;
(5) screening 4 instar insects with the specification of more than 130 mg/insect, continuously culturing prepupa on a culture bed, and collecting prepupa when the prepupa rate reaches 50-100% to obtain seed insects;
(6) the breeding insects are intensively placed in a container capable of vibrating, the container is vibrated every day, pupae with pupae on the surface are quantitatively placed in an eclosion basin, the eclosion basin is placed in an adult breeding room, and eclosion is waited;
(7) after eclosion of the imagoes, controlling the temperature in the imagoes breeding room to be 25-30 ℃ and the humidity to be 40-80%, attracting the mated imagoes to lay eggs on egg collecting objects by placing trapping objects beside the egg collecting objects, collecting the eggs every day and replacing the egg collecting objects.
According to the breeding method, the eggs in the protogut stage are selected when the eggs are collected, the eggs in the protogut stage are in the most stable state of black soldier fly embryo development, the damage to the black soldier fly eggs is the lowest when the eggs are collected, the tidiness and the activity of the larvae which are hatched initially are ensured, the hatching rate of the eggs is over 80%, and the survival rate of the seedlings (the larvae which are hatched initially are grown to 5 days) is over 50%; screening 5-day-old larva with a size of more than 20 mg/larva, developing tidily and actively, carrying out rotary disc brooding and screening 4-year-old larva with a size of more than 130 mg/larva to carry out pre-pupa cultivation, and ensuring that the size of the obtained larva is more than 200 mg/larva and the size of pupa is more than 160 mg/larva; the breeding method has the advantages that the yield of the breeding insects is 40-60% after the larvae are developed from the initial hatching, the obtained breeding insects have excellent quality, the egg laying amount per female is remarkably increased from 400 eggs per female with 300 eggs and 1000 eggs per female with 800 eggs compared with the breeding insects obtained by the non-standardized method, the obtained eggs have excellent quality, and the breeding method can be further used for breeding the hermetia illucens; the breeding process is standardized, so that the hermetia illucens can be stably bred in each batch.
Preferably, the incubation in step (1) and the incubation in step (2) are both performed in a climatic chamber. The most fragile insect-state ova are incubated in a climatic chamber, the environment is controllable, and the incubation rate is high and can reach more than 90%.
Preferably, when hatching in the step (2), the eggs in the gastral period are flatly laid on a grading sieve of 40-80 meshes, the thickness of the flatly laid eggs is not more than 3mm, the grading sieve is arranged in a tray, and powdery substances are added to the periphery (namely the edge) in the tray. The powdered material, such as corn flour, is added around the tray to prevent larvae from escaping, and the larvae hatched just before escape easily, but in the powdered material, the water on the surface of the larvae hatched just before is adsorbed, so that the larvae crawl slowly and cannot climb out of the tray. More preferably, the classifying screen used in the step (2) is 60 mesh.
Preferably, in the step (3), the culture medium is mainly prepared by mixing and fermenting bran, clear water, brown sugar and a bacillus subtilis solution, wherein the weight ratio of the bran to the clear water to the brown sugar to the bacillus subtilis solution is bran: clear water: brown sugar: bacillus subtilis solution ═ 1: 2.5-3.5: 0.05-0.2: 0.005-0.02. The brown sugar is added into the surface culture substrate, so that the nutrition which can be effectively absorbed is provided for the newly hatched larvae with poor vitality, and the survival rate of the young seedlings can be obviously improved. When the weight ratio of the bran, the clear water, the brown sugar and the bacillus subtilis solution is within the specific range, the survival rate of the 5-day-old larvae is higher than that outside the range, the survival rate can reach more than 60%, and the specification is larger.
Preferably, in the step (3), before the cultivation, a cultivation substrate residue generated when the black soldier fly is hatched to 5 days old in the last batch is inoculated into the cultivation substrate; in the step (4) and the step (5), before cultivation, the cultivation residues generated in the previous step are respectively inoculated on a cultivation bed. If the breeding residues are not inoculated, the larvae eat slowly and are easy to escape, and the larvae are not beneficial to harvesting and screening at each stage; the culture residues contain beneficial microbial flora accumulated by the larvae in the feeding process, if the culture residues are not inoculated, the larvae need to re-culture the flora, and the inoculated culture residues provide a proper environment for the larvae, so that the larvae are prevented from generating stress reaction, the development of the larvae can be promoted, and the survival rate is improved.
Preferably, when the qualified larvae are transferred to a breeding bed to be cultivated to 4-year-old insects in the step (4), the feed used comprises kitchen pulp and chicken feed. The kitchen slurry and the chicken feed are used in a matching manner, so that more comprehensive and rich nutrient substances can be provided for the development of the hermetia illucens larvae, and the hermetia illucens larvae can thrive.
Preferably, in the step (5), the feeding is only carried out in the middle of the culture bed during the culture process from 4-year-old insects to prepupa, and the feeding is kept dry for four weeks. In the process of cultivating the prepupa, only the feed is supplemented in the middle of the cultivating bed, the periphery is kept dry, so that the prepupa is concentrated around the cultivating bed, and the 4-instar insects continue to take the feed in the middle of the cultivating bed, thereby being convenient for collecting the prepupa.
Preferably, the breeding method further comprises the following steps: and (4) carrying out repeated treatment of the steps (1) to (7) on the worm eggs collected in the step (7).
Preferably, in the step (7), the density of the imagoes in the imago breeding chamber is controlled to be 800-3The male-female ratio is female: male 1-1.2: 1. the single female egg laying amount is relatively high under the condition of maintaining the density and the sex ratio of the adult insects in the shed at the specific ratio.
Preferably, between the step (5) and the step (6), further comprising the step (5 a): adding sawdust into the seed worms obtained in the step (5), and then storing in a refrigeration house.
Preferably, the freezer temperature is 8 ℃.
Preferably, the pre-pupal emergence into adults is carried out in sawdust. In the process from pupa prepending to eclosion into adults, the sawdust is adopted to provide multiple benefits for the insects, and the sawdust water content adopted in the cultivation process from pupa prepending to pupa is preferably 40-70%, and the sawdust water content adopted in the cultivation process from pupa to eclosion into adults is preferably 30-50%.
Compared with the prior art, the invention has the following advantages and beneficial effects: the method collects the eggs in the original intestinal period to hatch, has the lowest damage to the hermetia illucens eggs, ensures that the larvae which are hatched initially are tidy and active, and ensures that the hatching rate of the eggs is more than 80 percent, even more than 90 percent; meanwhile, screening 5-day-old larva rotary disc brooding with survival rate of more than 60 percent, specification of more than 20 mg/larva, and regular and active growth and screening 4-year-old larva with specification of more than 130 mg/larva for pre-pupa cultivation, ensuring the obtained seed size to be more than 200 mg/larva, the pupa size to be more than 160 mg/larva, and the yield from the development of the newly hatched larva to the seed insect to be 40-60 percent; the breeding process is standardized, so that the hermetia illucens can be stably bred in each batch; in addition, the obtained seed worms are used for spawning, so that the single-female spawning amount is obviously increased from 300-400 grains/female to 800-1000 grains/female.
Detailed Description
To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples.
Example 1
The embodiment is an implementation manner of the breeding method of the hermetia illucens, and the breeding method of the hermetia illucens comprises the following steps:
(1) and (3) worm egg development: collecting eggs produced in one day by using an egg collecting plate, placing the egg collecting plate in a climate box with the temperature of 28-30 ℃ and the humidity of 60% -80%, and after 24h, enabling the eggs to grow to the gastral period;
(2) harvesting worm eggs: scraping the eggs in the protogut stage from an egg collecting plate and weighing;
(3) hatching worm eggs: quantitatively spreading the eggs of the insects in the protogut period on a 60-mesh grading sieve, wherein the spreading thickness is not more than 3mm, then placing the grading sieve in a tray, scattering a small amount of corn flour on the periphery of the tray, placing the tray in a climate box with the temperature of 28-30 ℃ and the humidity of 60% -80% for hatching, dropping the primarily hatched larvae in the tray, and collecting the primarily hatched larvae;
(4) inoculating insects: quantitatively placing the primarily hatched larvae on a culture substrate (the culture substrate is mainly formed by mixing and fermenting bran, clear water, brown sugar and a bacillus subtilis solution, wherein the weight ratio of the bran to the clear water to the brown sugar to the bacillus subtilis solution is 1: 2.5: 0.05: 0.005);
(5) inoculating bacteria: covering a culture substrate residue generated by hatching the larvae which are hatched to 5-day-old larvae in the previous batch of black soldier flies above the larvae which are hatched primarily;
(6) seedling cultivation: culturing the larvae which are hatched for the first time after the inoculation of the insects and the bacteria in a seedling culture room with the temperature of 28-30 ℃ and the humidity of 60-80% to 5-day-old larvae;
(7) and (3) determining the survival rate of the seedlings: screening 5-day-old larvae with survival rate of more than 60%, specification of more than 20mg, and regular and active growth to obtain qualified larvae;
(8) a rotary table: quantitatively transferring qualified larvae and culture residues generated in the step (7) to a culture bed with a larger bottom area;
(9) brooding: feeds rich in nutrient substances such as kitchen pulp, chicken feed and the like are used for breeding qualified larvae to 4 instar insects;
(10) screening: screening the 4 th instar insects by using a screening machine, and screening out the 4 th instar insects with the specification of more than 130 mg;
(11) cultivating pre-pupae: after the screened 4-year-old insects with the specification of 130 mg/above are inoculated with the culture residues generated in the step (9) with equal weight, the prepupa is continuously cultured on a culture bed, the feeding is only carried out in the middle of the culture bed in the prepupa culture process, and the periphery is kept dry and comfortable;
(12) pre-pupa collection: when the prepupa rate reaches 80%, the prepupa are all in dry and comfortable positions around, the prepupa at the positions are collected and screened, and the rest white insects are eliminated, so that the seed insects are obtained;
(13) pupating: adding sawdust with water content of 50% into the seed insect, placing in a vibratable container for pupation, or placing in a freezer at 8 deg.C after adding sawdust, storing in a vibratable container for pupation, vibrating the container every day, taking pupate and sawdust with pupation surface, placing in an eclosion basin, placing the eclosion basin in an adult shed in batches by adopting a multi-batch pupation method, waiting for eclosion, and controlling the adult density in the adult shed to be 1000 pieces/m3The male-female ratio is female: male 1-1.2: 1;
(14) eclosion and adult management: maintaining the temperature of the adult greenhouse at 25-30 ℃, the humidity of 40-80% and the moisture of sawdust of 30-50%, automatically eclosion pupae, changing air for one or more times every day, and spraying water for one or two times;
(15) producing worm eggs: the middle of the adult greenhouse is provided with an egg collecting plate, and after the adult insects mate, the collection objects below the egg collecting plate attract the female insects to lay eggs in the egg collecting plate.
According to the breeding method of the hermetia illucens, the hatching rate of eggs is 92.34%, the survival rate of seedlings is 78.45%, the obtained seed worms are 232 mg/egg, the pupa size is 184 mg/egg, the yield of the seed worms is 46.78% after the larvae are hatched initially, and the egg laying amount per female is 982.
Example 2
The embodiment is an implementation manner of the breeding method of the hermetia illucens, and the weight ratio of the solutions except for bran, clear water, brown sugar and bacillus subtilis is as follows: clear water: brown sugar: bacillus subtilis solution ═ 1: 3: 0.1: the procedure was repeated except for 0.01 in the same manner as in example 1.
According to the breeding method of the hermetia illucens, the hatching rate of eggs is 91.78%, the survival rate of seedlings is 71.03%, the obtained seed worms are 235 mg/egg, the pupa size is 185 mg/egg, the yield of the seed worms is 54.94% after the larvae are hatched initially, and the egg laying amount per female egg is 975.
Example 3
The embodiment is an implementation manner of the breeding method of the hermetia illucens, and the weight ratio of the solutions except for bran, clear water, brown sugar and bacillus subtilis is as follows: clear water: brown sugar: bacillus subtilis solution ═ 1: 3.5: 0.2: except for 0.02, the procedure was as in example 1.
According to the breeding method of the hermetia illucens, the hatching rate of eggs is 91.93%, the survival rate of seedlings is 72.02%, the obtained seed worms are 225 mg/egg, the pupa size is 178 mg/egg, the yield of the seed worms is 54.05% after the first hatching of the larvae, and the egg laying amount of a single female is 972 eggs/female.
Comparative example 1
The comparative example provides a breeding method of hermetia illucens, and the breeding method of the hermetia illucens in the comparative example comprises the following steps of removing bran, clear water, brown sugar and a bacillus subtilis solution by weight: clear water: brown sugar: bacillus subtilis solution ═ 1: 2.5: 0.02: 0.005, the same as example 1. The survival rate of seedlings is 52.16%, the obtained seed worms have a specification of more than 220 mg/worm, the pupa have a specification of 165 mg/worm, the yield of the seed worms is 40.78% after the larva is hatched initially, and the egg laying amount of single female is 981/worm.
Comparative example 2
The comparative example provides a breeding method of hermetia illucens, and the breeding method of the hermetia illucens in the comparative example comprises the following steps of removing bran, clear water, brown sugar and a bacillus subtilis solution by weight: clear water: brown sugar: bacillus subtilis solution ═ 1: 3.5: 0.5: 0.02, the same procedure as in example 1 was repeated. The survival rate of seedlings is 65.18%, the obtained seed worms have the specification of more than 225 mg/worm, the pupa have the specification of 168 mg/worm, the yield of the seed worms is 45.02% after the larva is hatched initially, and the egg laying amount of single female is 972 grains/female.
Comparative example 3
The comparative example provides a breeding method of hermetia illucens, which maintains the adult density in the shed to be 800-3The male-female ratio is female: male-0.8: the procedure of example 1 was repeated except that the above-mentioned procedure was repeated. The egg laying amount per female was 742 pieces/female.
Comparative example 4
The comparative example provides a breeding method of hermetia illucens, which maintains the adult density in the shed to be 800-3The male-female ratio is female: male 1.4: the procedure of example 1 was repeated except that the above-mentioned procedure was repeated. The egg laying amount per female is 785 eggs/female.
Comparative example 5
The comparative example provides a breeding method of hermetia illucens, which maintains the adult density in the shed to be 700-3Otherwise, the same procedure as in example 1 was repeated. The egg laying amount per female is 992 eggs/female. The single-female egg laying amount of the comparative example 5 is similar to that of the example 1, but the utilization rate of the adult greenhouse of the example 1 is higher, and the construction cost of the adult greenhouse and the labor cost for harvesting the eggs are reduced.
Comparative example 6
The comparative example provides a breeding method of hermetia illucens, which maintains the adult density in the shed to be 1050-3Otherwise, the same procedure as in example 1 was repeated. The egg laying amount per female is 842 grains/female. The density of the insects in the adult greenhouse cannot be too high, otherwise the egg laying amount of single female can be reduced.
Comparative example 7
The comparative example provides a breeding method of hermetia illucens, which is the same as that in example 1 except that inoculation treatment is not carried out. The survival rate of seedlings is 69.78%, the obtained seed worms are 208 mg/worm, the pupa size is 145 mg/worm, the yield of the seed worms is 40.78% after the larva is hatched initially, the egg laying amount per female per worm is 925, the pupa prepuperation period is prolonged by 6-8 days compared with that of example 1, and the difficulty in collecting the pupa prepupinations is increased.
It should be finally noted that the above embodiments are only intended to illustrate the technical solutions of the present invention and not to limit the scope of the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (10)
1. The breeding method of the hermetia illucens is characterized by comprising the following steps of:
(1) culturing ovum in 28-30 deg.C environment with humidity of 60-80% to original intestinal stage, and collecting ovum in original intestinal stage;
(2) hatching the eggs in the protogut period in an environment with the temperature of 28-30 ℃ and the humidity of 60-80%, and collecting the primarily hatched larvae;
(3) quantitatively placing the primarily hatched larvae on a culture substrate, culturing in an environment with the temperature of 28-30 ℃ and the humidity of 60-80%, and screening 5-day-old larvae with the specification of more than 20mg, regular growth and activity when the primarily hatched larvae grow to 5 days old to obtain qualified larvae;
(4) quantitatively transferring the qualified larvae to a culture bed to culture the larvae to 4 instar insects;
(5) screening 4 instar insects with the specification of more than 130 mg/insect, continuously culturing prepupa on a culture bed, and collecting prepupa when the prepupa rate reaches 50-100% to obtain seed insects;
(6) the breeding insects are intensively placed in a container capable of vibrating, the container is vibrated every day, pupae with pupae on the surface are quantitatively placed in an eclosion basin, the eclosion basin is placed in an adult breeding room, and eclosion is waited;
(7) after eclosion of the imagoes, controlling the temperature in the imagoes breeding room to be 25-30 ℃ and the humidity to be 40-80%, attracting the mated imagoes to lay eggs on egg collecting objects by placing trapping objects beside the egg collecting objects, collecting the eggs every day and replacing the egg collecting objects.
2. The method for breeding hermetia illucens according to claim 1, wherein the breeding in step (1) and the hatching in step (2) are performed in a climatic chamber.
3. The method for breeding hermetia illucens according to claim 1, wherein the eggs in the gastral stage are laid on a grading sieve of 40-80 meshes with a thickness of not more than 3mm when incubated in the step (2), the grading sieve is placed in a tray, and powdered substances are added to the periphery of the tray.
4. The breeding method of hermetia illucens according to claim 1, wherein in the step (3), the culture medium is mainly prepared by mixing and fermenting bran, clear water, brown sugar and a bacillus subtilis solution, wherein the weight ratio of the bran, the clear water, the brown sugar and the bacillus subtilis solution is 1: 2.5-3.5: 0.05-0.2: 0.005-0.02.
5. The breeding method of hermetia illucens according to claim 1, wherein in the step (3), before the breeding, breeding substrate residues generated when the hermetia illucens hatched in the first time of the previous batch are hatched to 5 days old are inoculated into the breeding substrate; in the step (4) and the step (5), before cultivation, the cultivation residues generated in the previous step are respectively inoculated on a cultivation bed.
6. The breeding method of hermetia illucens according to claim 1, wherein the feed used in the step (4) of transferring the qualified larvae to a breeding bed for breeding to 4-year-old insects comprises kitchen pulp and chicken feed.
7. The breeding method of hermetia illucens according to claim 1, wherein in the step (5), the feeding is performed only in the middle of the breeding bed during the breeding process from 4 instar to prepupa, and the feeding is kept dry for four weeks.
8. The method for breeding hermetia illucens according to claim 1, further comprising the steps of: and (4) carrying out repeated treatment of the steps (1) to (7) on the worm eggs collected in the step (7).
9. According toThe method for breeding hermetia illucens as claimed in claim 1, wherein in said step (7), the density of adult insects in said adult breeding chamber is controlled to be 800-3The male-female ratio is female: male 1-1.2: 1.
10. the method for breeding hermetia illucens according to claim 1, wherein between the step (5) and the step (6), the method further comprises the step (5 a): adding sawdust into the seed worms obtained in the step (5), and then storing in a refrigeration house.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112450156A (en) * | 2020-12-10 | 2021-03-09 | 河南农业大学 | Method for efficiently converting organic solid waste by using high-density hermetia illucens |
| CN112514856A (en) * | 2020-11-30 | 2021-03-19 | 广东省农业科学院动物卫生研究所 | Prevent insect feeding dish that heisui river horsefly larva escaped |
| CN113875701A (en) * | 2021-09-30 | 2022-01-04 | 广州安芮洁环保科技有限公司 | Method for adjusting proportion of hermetia illucens male and female prepupa by using feed |
| NO20210318A1 (en) * | 2021-03-11 | 2022-09-12 | David Manoocher Tehrani | A PROCEDURE FOR RAising BLACK SOLDIER FLY LARVAE |
| CN116114663A (en) * | 2023-02-22 | 2023-05-16 | 深圳安芮洁环保科技有限公司 | Black soldier fly breeding method |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101889629A (en) * | 2010-06-13 | 2010-11-24 | 中山大学 | Method for processing food waste by using black soldier fly larvae and material formula |
| CN101978823A (en) * | 2010-11-25 | 2011-02-23 | 广东省昆虫研究所 | Method for artificially feeding black soldier flies |
| CN102329157A (en) * | 2011-07-07 | 2012-01-25 | 中山大学 | Method for processing human excrement by utilizing black solider flies to prepare organic fertilizer |
| EP2646398A2 (en) * | 2010-09-03 | 2013-10-09 | Bioconversion SRL | Plant and method for organic waste bioconversion and municipal waste biostabilization by means of insect larvae |
| CN106305631A (en) * | 2016-08-25 | 2017-01-11 | 仇颖超 | Breeding method of high-protein Hermetia illucens pupae |
| CN106614398A (en) * | 2016-12-19 | 2017-05-10 | 江苏大学 | Method for performing pig manure resource treatment by utilizing hermetia illucens |
| CN107716508A (en) * | 2017-09-19 | 2018-02-23 | 广东省生物资源应用研究所 | A kind of method using black soldier flies bioconversion kitchen garbage |
| CN109757627A (en) * | 2019-03-26 | 2019-05-17 | 四川十分美环境卫生管理有限公司 | A kind of feed and preparation method that can promote the growth of black soldier flies larva |
| CN109874747A (en) * | 2019-02-28 | 2019-06-14 | 广州安芮洁环保科技有限公司 | Black soldier flies larva cultivation equipment and method |
| KR20200113274A (en) * | 2018-02-07 | 2020-10-06 | 인섹트 테크놀로지 그룹 리서치 유케이 리미티드 | Apparatus and method for breeding and collecting insect larvae |
-
2020
- 2020-06-24 CN CN202010595543.8A patent/CN111742889A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101889629A (en) * | 2010-06-13 | 2010-11-24 | 中山大学 | Method for processing food waste by using black soldier fly larvae and material formula |
| EP2646398A2 (en) * | 2010-09-03 | 2013-10-09 | Bioconversion SRL | Plant and method for organic waste bioconversion and municipal waste biostabilization by means of insect larvae |
| CN101978823A (en) * | 2010-11-25 | 2011-02-23 | 广东省昆虫研究所 | Method for artificially feeding black soldier flies |
| CN102329157A (en) * | 2011-07-07 | 2012-01-25 | 中山大学 | Method for processing human excrement by utilizing black solider flies to prepare organic fertilizer |
| CN106305631A (en) * | 2016-08-25 | 2017-01-11 | 仇颖超 | Breeding method of high-protein Hermetia illucens pupae |
| CN106614398A (en) * | 2016-12-19 | 2017-05-10 | 江苏大学 | Method for performing pig manure resource treatment by utilizing hermetia illucens |
| CN107716508A (en) * | 2017-09-19 | 2018-02-23 | 广东省生物资源应用研究所 | A kind of method using black soldier flies bioconversion kitchen garbage |
| KR20200113274A (en) * | 2018-02-07 | 2020-10-06 | 인섹트 테크놀로지 그룹 리서치 유케이 리미티드 | Apparatus and method for breeding and collecting insect larvae |
| CN109874747A (en) * | 2019-02-28 | 2019-06-14 | 广州安芮洁环保科技有限公司 | Black soldier flies larva cultivation equipment and method |
| CN109757627A (en) * | 2019-03-26 | 2019-05-17 | 四川十分美环境卫生管理有限公司 | A kind of feed and preparation method that can promote the growth of black soldier flies larva |
Non-Patent Citations (2)
| Title |
|---|
| 莫文艳等: "黑水虻卵胚胎发育过程及在生产上的应用", 《环境昆虫学报》 * |
| 靳任任等: "黑水虻繁育技术", 《农村新技术》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112514856A (en) * | 2020-11-30 | 2021-03-19 | 广东省农业科学院动物卫生研究所 | Prevent insect feeding dish that heisui river horsefly larva escaped |
| CN112450156A (en) * | 2020-12-10 | 2021-03-09 | 河南农业大学 | Method for efficiently converting organic solid waste by using high-density hermetia illucens |
| NO20210318A1 (en) * | 2021-03-11 | 2022-09-12 | David Manoocher Tehrani | A PROCEDURE FOR RAising BLACK SOLDIER FLY LARVAE |
| CN113875701A (en) * | 2021-09-30 | 2022-01-04 | 广州安芮洁环保科技有限公司 | Method for adjusting proportion of hermetia illucens male and female prepupa by using feed |
| CN116114663A (en) * | 2023-02-22 | 2023-05-16 | 深圳安芮洁环保科技有限公司 | Black soldier fly breeding method |
| CN116114663B (en) * | 2023-02-22 | 2024-04-09 | 深圳安芮洁环保科技有限公司 | Black soldier fly breeding method |
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