ZA962836B - Method of reducing the formation of artifacts. - Google Patents

Method of reducing the formation of artifacts.

Info

Publication number
ZA962836B
ZA962836B ZA9602836A ZA962836A ZA962836B ZA 962836 B ZA962836 B ZA 962836B ZA 9602836 A ZA9602836 A ZA 9602836A ZA 962836 A ZA962836 A ZA 962836A ZA 962836 B ZA962836 B ZA 962836B
Authority
ZA
South Africa
Prior art keywords
pct
artifacts
formation
reducing
transcribing
Prior art date
Application number
ZA9602836A
Other languages
English (en)
Inventor
Peter Bauer
Arndt Dr Rolfs
Vera Dr Regit-Zagrosek
Eckart Prof Dr Fleck
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Publication of ZA962836B publication Critical patent/ZA962836B/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pyrane Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Lighting Device Outwards From Vehicle And Optical Signal (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
ZA9602836A 1995-04-11 1996-04-10 Method of reducing the formation of artifacts. ZA962836B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19513728A DE19513728A1 (de) 1995-04-11 1995-04-11 Verfahren zur Reduzierung der Bildung von Artefakten

Publications (1)

Publication Number Publication Date
ZA962836B true ZA962836B (en) 1997-10-10

Family

ID=7759479

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA9602836A ZA962836B (en) 1995-04-11 1996-04-10 Method of reducing the formation of artifacts.

Country Status (10)

Country Link
US (1) US6124093A (fr)
EP (1) EP0871769B1 (fr)
JP (1) JP4126348B2 (fr)
AT (1) ATE205544T1 (fr)
AU (1) AU5335596A (fr)
DE (2) DE19513728A1 (fr)
ES (1) ES2162044T3 (fr)
IL (1) IL117880A0 (fr)
WO (1) WO1996032501A1 (fr)
ZA (1) ZA962836B (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9716664D0 (en) * 1997-08-06 1997-10-15 Norwegian Inst Of Fisheries & A method of removing nucleic acid contamination reactions

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5561058A (en) * 1986-08-22 1996-10-01 Hoffmann-La Roche Inc. Methods for coupled high temperatures reverse transcription and polymerase chain reactions
US5798257A (en) * 1990-07-09 1998-08-25 Research Corporation Technologies, Inc. Nucleic acid encoding human MTS-1 protein
FR2690691B1 (fr) * 1992-04-29 1999-02-12 Bio Merieux Procede d'amplification d'arn necessitant une seule etape de manipulation.
WO1994012657A1 (fr) * 1992-11-20 1994-06-09 Katcher Harold L Methodes de pcr par adjonction directe de phenol, de transcription inverse (tr) et de pcr-tr
EP0632134B1 (fr) * 1993-07-01 2001-09-05 F. Hoffmann-La Roche Ag Réactif et procédé pour la transcription inverse à haute température suivi par la réaction en chaíne de la polymérase

Also Published As

Publication number Publication date
WO1996032501A1 (fr) 1996-10-17
IL117880A0 (en) 1996-12-05
US6124093A (en) 2000-09-26
AU5335596A (en) 1996-10-30
EP0871769B1 (fr) 2001-09-12
DE59607694D1 (de) 2001-10-18
DE19513728A1 (de) 1996-10-31
JP4126348B2 (ja) 2008-07-30
ATE205544T1 (de) 2001-09-15
ES2162044T3 (es) 2001-12-16
EP0871769A1 (fr) 1998-10-21
JPH11503325A (ja) 1999-03-26

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