ZA9610456B - N-terminally extended proteins expressed in yeast - Google Patents

N-terminally extended proteins expressed in yeast

Info

Publication number
ZA9610456B
ZA9610456B ZA9610456A ZA9610456A ZA9610456B ZA 9610456 B ZA9610456 B ZA 9610456B ZA 9610456 A ZA9610456 A ZA 9610456A ZA 9610456 A ZA9610456 A ZA 9610456A ZA 9610456 B ZA9610456 B ZA 9610456B
Authority
ZA
South Africa
Prior art keywords
yeast
terminally extended
proteins expressed
dna
extended proteins
Prior art date
Application number
ZA9610456A
Other languages
English (en)
Inventor
Thomas Borglum Kjeldsen
Per Balschmidt
Annette Frost Pettersson
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of ZA9610456B publication Critical patent/ZA9610456B/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Diabetes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
ZA9610456A 1995-12-20 1996-12-12 N-terminally extended proteins expressed in yeast ZA9610456B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK144995 1995-12-20

Publications (1)

Publication Number Publication Date
ZA9610456B true ZA9610456B (en) 1997-06-20

Family

ID=8104944

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA9610456A ZA9610456B (en) 1995-12-20 1996-12-12 N-terminally extended proteins expressed in yeast

Country Status (11)

Country Link
EP (1) EP0868523B1 (zh)
JP (1) JP4180112B2 (zh)
CN (1) CN1125181C (zh)
AT (1) ATE295890T1 (zh)
AU (1) AU1093597A (zh)
CA (1) CA2241367C (zh)
DE (1) DE69634757T2 (zh)
ES (1) ES2242969T3 (zh)
IL (1) IL124939A (zh)
WO (1) WO1997022706A1 (zh)
ZA (1) ZA9610456B (zh)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1365028B1 (en) 1996-12-13 2009-03-18 Novartis Vaccines and Diagnostics, Inc. Method for expression of PDGF or IGF proteins in yeast
AU9162398A (en) * 1997-08-22 1999-03-16 Boehringer Mannheim Gmbh Process for producing insulin precursor proteins
DK1947187T5 (da) * 2000-02-28 2011-10-24 Novartis Vaccines & Diagnostic Hybrid ekspression af neisserial-proteiner
WO2002085932A2 (en) * 2001-04-24 2002-10-31 Innogenetics N.V. Constructs and methods for expression of recombinant hcv envelope proteins
US7595172B2 (en) 2001-07-24 2009-09-29 Novo Nordisk A/S Method for making acylated polypeptides
WO2005121333A1 (en) * 2004-06-14 2005-12-22 Novozymes A/S Signal peptide for producing a polypeptide
GB201008682D0 (en) 2010-05-25 2010-07-07 Vib Vzw Epitope tag for affinity based applications
CN111560379B (zh) * 2020-05-26 2022-09-13 北京惠之衡生物科技有限公司 密码子优化的门冬胰岛素前体基因、重组载体、基因工程菌及其应用
CN113249241B (zh) * 2021-06-21 2021-10-15 奥锐特药业(天津)有限公司 一种酿酒酵母蛋白酶缺失菌株的构建及其应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3382547D1 (de) * 1983-01-12 1992-05-27 Chiron Corp Sekretorische expression in eukaryoten.
FR2593518B1 (fr) * 1985-05-02 1989-09-08 Transgene Sa Vecteurs d'expression et de secretion de l'hirudine par les levures transformees
CA1340772C (en) * 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
DK105489D0 (da) * 1989-03-03 1989-03-03 Novo Nordisk As Polypeptid
DK82893D0 (da) * 1993-07-08 1993-07-08 Novo Nordisk As Peptid
US5639642A (en) * 1994-06-16 1997-06-17 Novo Nordisk A/S Synthetic leader peptide sequences
IL114160A (en) * 1994-06-17 2006-12-31 Novo Nordisk As Dna constructs encoding heterologous proteins and processes for the heterologous protein production in yeast

Also Published As

Publication number Publication date
ATE295890T1 (de) 2005-06-15
CA2241367C (en) 2007-02-06
JP2000501617A (ja) 2000-02-15
EP0868523A1 (en) 1998-10-07
IL124939A0 (en) 1999-01-26
DE69634757D1 (de) 2005-06-23
AU1093597A (en) 1997-07-14
EP0868523B1 (en) 2005-05-18
WO1997022706A1 (en) 1997-06-26
JP4180112B2 (ja) 2008-11-12
ES2242969T3 (es) 2005-11-16
CN1207773A (zh) 1999-02-10
CA2241367A1 (en) 1997-06-26
IL124939A (en) 2007-06-03
CN1125181C (zh) 2003-10-22
DE69634757T2 (de) 2006-02-02

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