ZA200407146B - Melting temperature dependent dna amplification - Google Patents
Melting temperature dependent dna amplification Download PDFInfo
- Publication number
- ZA200407146B ZA200407146B ZA200407146A ZA200407146A ZA200407146B ZA 200407146 B ZA200407146 B ZA 200407146B ZA 200407146 A ZA200407146 A ZA 200407146A ZA 200407146 A ZA200407146 A ZA 200407146A ZA 200407146 B ZA200407146 B ZA 200407146B
- Authority
- ZA
- South Africa
- Prior art keywords
- nucleic acid
- target nucleic
- amplification
- melting temperature
- species
- Prior art date
Links
- 238000002844 melting Methods 0.000 title claims description 65
- 230000008018 melting Effects 0.000 title claims description 65
- 230000004544 DNA amplification Effects 0.000 title description 4
- 230000001419 dependent effect Effects 0.000 title description 2
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- 108020004707 nucleic acids Proteins 0.000 claims description 163
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- 230000003321 amplification Effects 0.000 claims description 130
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- 238000000034 method Methods 0.000 claims description 67
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AUPS0769A AUPS076902A0 (en) | 2002-02-26 | 2002-02-26 | Novel selective polymerase chain reaction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ZA200407146B true ZA200407146B (en) | 2006-07-26 |
Family
ID=3834368
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ZA200407146A ZA200407146B (en) | 2002-02-26 | 2004-09-07 | Melting temperature dependent dna amplification |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20080044812A1 (fr) |
| EP (1) | EP1485505A4 (fr) |
| JP (1) | JP2005518216A (fr) |
| CN (1) | CN1650028A (fr) |
| AU (1) | AUPS076902A0 (fr) |
| CA (1) | CA2477574A1 (fr) |
| WO (1) | WO2003072809A1 (fr) |
| ZA (1) | ZA200407146B (fr) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9487823B2 (en) | 2002-12-20 | 2016-11-08 | Qiagen Gmbh | Nucleic acid amplification |
| CA2579741A1 (fr) * | 2004-09-20 | 2006-03-30 | University Of Pittsburgh-Of The Commonwealth System Of Higher Education | Procede de refroidissement d'une reaction multiplexee multimode |
| EP1762627A1 (fr) | 2005-09-09 | 2007-03-14 | Qiagen GmbH | Procédé pour l'activation d'acides nucléiques pour effectuer une réaction d'une polymérase |
| EP2574681B1 (fr) | 2007-03-28 | 2016-03-23 | Signal Diagnostics | Système et procédé d'analyse à haute résolution d'acides nucléiques pour détecter des variations de séquence |
| JP5531367B2 (ja) * | 2007-08-01 | 2014-06-25 | ダナ−ファーバー キャンサー インスチテュート | 標的配列の濃縮 |
| WO2010001969A1 (fr) | 2008-07-02 | 2010-01-07 | アークレイ株式会社 | Procédé d'amplification d'une séquence d'acides nucléiques cible, procédé de détection de la mutation à l'aide du procédé, et réactifs en vue d'une utilisation dans les procédés |
| US10669574B2 (en) | 2008-11-18 | 2020-06-02 | XCR Diagnostics, Inc. | DNA amplification technology |
| KR101649179B1 (ko) | 2010-01-21 | 2016-08-19 | 아크레이 가부시키가이샤 | 표적 서열의 증폭 방법, 다형 검출 방법 및 그것에 사용하는 시약 |
| DK2545189T3 (en) | 2010-03-08 | 2018-04-23 | Dana Farber Cancer Inst Inc | COMPLETE COLD-PCR ENRICHMENT WITH REFERENCE BLOCKING SEQUENCE |
| EP3333269B1 (fr) | 2011-03-31 | 2021-05-05 | Dana-Farber Cancer Institute, Inc. | Procédés permettant d'activer un multiplexage froid-pcr |
| US9133490B2 (en) | 2012-05-16 | 2015-09-15 | Transgenomic, Inc. | Step-up method for COLD-PCR enrichment |
| US10913977B2 (en) | 2013-07-24 | 2021-02-09 | Dana-Farber Cancer Institute, Inc. | Methods and compositions to enable enrichment of minor DNA alleles by limiting denaturation time in PCR or simply enable enrichment of minor DNA alleles by limiting the denaturation time in PCR |
| WO2015054516A2 (fr) | 2013-10-09 | 2015-04-16 | Fluoresentric, Inc. | Sondes multiplexées |
| US20160273022A1 (en) | 2013-10-20 | 2016-09-22 | Trovagene, Inc. | Synthesis and enrichment of nucleic acid sequences |
| CA2954420A1 (fr) * | 2014-07-10 | 2016-01-14 | Fluoresentric, Inc. | Technologie d'amplification d'adn |
| EA038921B9 (ru) | 2015-05-18 | 2021-12-06 | Сага Диагностикс Аб | Обнаружение целевой нуклеиновой кислоты и ее вариантов |
| CN107338240B (zh) * | 2015-11-25 | 2020-11-24 | 葛猛 | 对样品中目标核酸序列进行偏向扩增的方法和试剂盒 |
| WO2018111835A1 (fr) | 2016-12-12 | 2018-06-21 | Dana-Farber Cancer Institute, Inc. | Compositions et procédés pour le codage par code-barres moléculaire de molécules d'adn avant l'enrichissement des mutations et/ou la détection des mutations |
| WO2019023243A1 (fr) | 2017-07-24 | 2019-01-31 | Dana-Farber Cancer Institute, Inc. | Procédés et compositions pour sélectionner et amplifier des cibles d'adn dans un mélange de réaction unique |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5310652A (en) * | 1986-08-22 | 1994-05-10 | Hoffman-La Roche Inc. | Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription |
| US5994056A (en) * | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| US5612473A (en) * | 1996-01-16 | 1997-03-18 | Gull Laboratories | Methods, kits and solutions for preparing sample material for nucleic acid amplification |
-
2002
- 2002-02-26 AU AUPS0769A patent/AUPS076902A0/en not_active Abandoned
-
2003
- 2003-02-26 EP EP03742899A patent/EP1485505A4/fr not_active Withdrawn
- 2003-02-26 CA CA002477574A patent/CA2477574A1/fr not_active Abandoned
- 2003-02-26 CN CNA038093642A patent/CN1650028A/zh active Pending
- 2003-02-26 JP JP2003571489A patent/JP2005518216A/ja active Pending
- 2003-02-26 WO PCT/AU2003/000243 patent/WO2003072809A1/fr active IP Right Grant
- 2003-02-26 US US10/505,773 patent/US20080044812A1/en not_active Abandoned
-
2004
- 2004-09-07 ZA ZA200407146A patent/ZA200407146B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| AUPS076902A0 (en) | 2002-03-21 |
| EP1485505A4 (fr) | 2007-06-06 |
| CN1650028A (zh) | 2005-08-03 |
| JP2005518216A (ja) | 2005-06-23 |
| US20080044812A1 (en) | 2008-02-21 |
| EP1485505A1 (fr) | 2004-12-15 |
| CA2477574A1 (fr) | 2003-09-04 |
| WO2003072809A1 (fr) | 2003-09-04 |
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