ZA200108397B - In vivo selection method for determining inhibitory RNA molecules. - Google Patents

In vivo selection method for determining inhibitory RNA molecules.

Info

Publication number
ZA200108397B
ZA200108397B ZA200108397A ZA200108397A ZA200108397B ZA 200108397 B ZA200108397 B ZA 200108397B ZA 200108397 A ZA200108397 A ZA 200108397A ZA 200108397 A ZA200108397 A ZA 200108397A ZA 200108397 B ZA200108397 B ZA 200108397B
Authority
ZA
South Africa
Prior art keywords
nucleotide sequence
binding
sequences
encoding
selection method
Prior art date
Application number
ZA200108397A
Other languages
English (en)
Inventor
Kyri Mitrophanous
Narry Kim
Ekaterini Kotsopoulou
Original Assignee
Oxford Biomedica Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oxford Biomedica Ltd filed Critical Oxford Biomedica Ltd
Publication of ZA200108397B publication Critical patent/ZA200108397B/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
ZA200108397A 1999-06-03 2001-10-12 In vivo selection method for determining inhibitory RNA molecules. ZA200108397B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB9912965.2A GB9912965D0 (en) 1999-06-03 1999-06-03 In vivo selection method

Publications (1)

Publication Number Publication Date
ZA200108397B true ZA200108397B (en) 2002-08-28

Family

ID=10854706

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA200108397A ZA200108397B (en) 1999-06-03 2001-10-12 In vivo selection method for determining inhibitory RNA molecules.

Country Status (12)

Country Link
US (2) US20020147169A1 (ja)
EP (1) EP1180167B1 (ja)
JP (1) JP2003501070A (ja)
KR (1) KR20020008178A (ja)
CN (1) CN1353769A (ja)
AT (1) ATE354676T1 (ja)
AU (1) AU762957B2 (ja)
CA (1) CA2374664A1 (ja)
DE (1) DE60033517T2 (ja)
GB (2) GB9912965D0 (ja)
WO (1) WO2000075370A1 (ja)
ZA (1) ZA200108397B (ja)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE552859T1 (de) * 2000-09-13 2012-04-15 Praecis Pharm Inc Pharmazeutische formulierungen zur kontinuierlichen abgabe von peptiden
WO2002022080A2 (en) * 2000-09-15 2002-03-21 Merck & Co., Inc. Enhanced first generation adenovirus vaccines expressing codon optimized hiv1-gag, pol, nef and modifications
EP1504108B1 (en) 2002-02-01 2013-04-17 Oxford Biomedica (UK) Limited Lentiviral vector
US7928216B2 (en) * 2004-04-29 2011-04-19 Yale University Nuclease resistant external guide sequences for treating inflammatory and viral related respiratory diseases
US11767534B2 (en) 2011-05-04 2023-09-26 The Broad Institute, Inc. Multiplexed genetic reporter assays and compositions

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5624824A (en) * 1989-03-24 1997-04-29 Yale University Targeted cleavage of RNA using eukaryotic ribonuclease P and external guide sequence
DE69131942T2 (de) * 1990-07-16 2000-06-29 New York Health Res Inst Auswahl von effizient ziel-rns spaltenden ribozymen
US6569432B1 (en) * 1995-02-24 2003-05-27 Sloan-Kettering Institute For Cancer Research Prostate-specific membrane antigen and uses thereof
US5869248A (en) * 1994-03-07 1999-02-09 Yale University Targeted cleavage of RNA using ribonuclease P targeting and cleavage sequences
AUPM516994A0 (en) * 1994-04-20 1994-05-12 Gene Shears Pty. Limited An in vivo gene expression system
JPH10504448A (ja) * 1994-06-15 1998-05-06 ザ・トラスティーズ・オブ・コランビア・ユニバーシティー・イン・ザ・シティー・オブ・ニューヨーク 腫瘍抑制遺伝子を同定する方法
DE4424762C1 (de) * 1994-07-04 1995-07-27 Max Planck Gesellschaft Ribozym-Bibliothek, ihre Herstellung und ihre Verwendung
DE4424761C1 (de) * 1994-07-04 1995-06-08 Max Planck Gesellschaft Expressionskassette für die Antisense- und die Ribozym-Expression
US6130092A (en) * 1994-07-04 2000-10-10 Max-Planck Gesellschaft Zur Forderung Der Wissenschaften E.V. Ribozyme gene library and method for making
DE69636122T2 (de) * 1995-11-13 2006-12-07 Takara Bio Inc., Otsu Verfahren zum gentransfer in zielzellen mit einem retrovirus
AU3984497A (en) * 1996-08-16 1998-03-06 Innovir Laboratories, Inc. Phenotypic conversion of cells mediated by external guide sequences
IL131746A0 (en) * 1997-03-14 2001-03-19 Method for integrating genes at specific sites in mammalian cells via homologous recombination and vectors for accomplishing the same
AU732321B2 (en) * 1997-11-21 2001-04-12 Yale University Method for identifying and inhibiting functional nucleic acid molecules in cells
DE69938128D1 (de) * 1998-03-28 2008-03-27 Univ Utah Res Found Zielgerichtete antisense-bibliotheken
US6156515A (en) * 1999-02-09 2000-12-05 Urocor, Inc. Prostate-specific gene for diagnosis, prognosis and management of prostate cancer
GB9906177D0 (en) * 1999-03-17 1999-05-12 Oxford Biomedica Ltd Anti-viral vectors
US20020002278A1 (en) * 1999-11-05 2002-01-03 Timothy W. Nilsen Random intracellular method for obtaining optimally active nucleic acid molecules

Also Published As

Publication number Publication date
AU5093500A (en) 2000-12-28
WO2000075370A1 (en) 2000-12-14
ATE354676T1 (de) 2007-03-15
KR20020008178A (ko) 2002-01-29
DE60033517T2 (de) 2007-10-25
JP2003501070A (ja) 2003-01-14
GB0124449D0 (en) 2001-12-05
EP1180167A1 (en) 2002-02-20
US20050221491A1 (en) 2005-10-06
US20020147169A1 (en) 2002-10-10
AU762957B2 (en) 2003-07-10
EP1180167B1 (en) 2007-02-21
GB9912965D0 (en) 1999-08-04
DE60033517D1 (de) 2007-04-05
CA2374664A1 (en) 2000-12-14
CN1353769A (zh) 2002-06-12
GB2364775A (en) 2002-02-06

Similar Documents

Publication Publication Date Title
Garber et al. Transcription of a cloned Bombyx mori tRNA2Ala gene: nucleotide sequence of the tRNA precursor and its processing in vitro
Bram et al. The ribonuclease III site flanking 23S sequences in the 30S ribosomal precursor RNA of E. coli
Sakonju et al. A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5′ border of the region
Jacq et al. A pseudogene structure in 5S DNA of Xenopus laevis
Young Transcription termination in the Escherichia coli ribosomal RNA operon rrnC.
Hui et al. Characterization of the ribosomal RNA gene clusters in Halobacterium cutirubrum.
Lockard et al. Mapping tRNA structure in solution using double-strand-specific ribonuclease V 1 from cobra venom
Borgatti et al. Transcription factor decoy molecules based on a peptide nucleic acid (PNA)-DNA chimera mimicking Sp1 binding sites
Nicholson et al. Promoter specificity of sigma G-containing RNA polymerase from sporulating cells of Bacillus subtilis: identification of a group of forespore-specific promoters
BR0316435A (pt) Produtos genéticos expressos diferencialmente em tumores e uso dos mesmos
AU2002352108A8 (en) Nucleic acid linkers and use thereof in gene synthesis
CA2175797A1 (en) Human kunitz-type protease inhibitors
HK1155477A1 (en) Modification of rna resulting in improved transcript stability and translation efficiency rna
Mackie Nucleotide sequence of the gene for ribosomal protein S20 and its flanking regions.
AU2003256615A8 (en) Methods and compositions relating to gene silencing
IN2014DN02484A (ja)
Wallace et al. The araC gene of Escherichia coli: transcriptional and translational start-points and complete nucleotide sequence
Martin et al. Primary structure of yeast mitochondrial DNA-coded phenylalanine-tRNA
ID27760A (id) Poliketida-poliketida, pembuatannya, dan bahan-bahan untuk penggunaannya
DE69734512D1 (de) Steigerung der genexpression
GB0124449D0 (en) In vivo selection method for determining inhibitory RNA molecules
Dahlberg et al. Sequence of the promoter-operator proximal region of the major leftward RNA of bacteriophage lambda
Rosa Structure analysis of three T7 late mRNA ribosome binding sites
WO1999024573A3 (en) SOYBEAN HOMOLOG OF SEED-SPECIFIC TRANSCRIPTION ACTIVATOR FROM $i(PHASEOLUS VULGARIS)
WO1996029407A3 (en) Cellubrevin homologs