ZA200106034B - A blood and organic stain remover. - Google Patents
A blood and organic stain remover. Download PDFInfo
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- ZA200106034B ZA200106034B ZA200106034A ZA200106034A ZA200106034B ZA 200106034 B ZA200106034 B ZA 200106034B ZA 200106034 A ZA200106034 A ZA 200106034A ZA 200106034 A ZA200106034 A ZA 200106034A ZA 200106034 B ZA200106034 B ZA 200106034B
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- ZA
- South Africa
- Prior art keywords
- blood
- spot remover
- organic
- organic spot
- remover
- Prior art date
Links
- 239000008280 blood Substances 0.000 title claims description 59
- 210000004369 blood Anatomy 0.000 title claims description 59
- 102000004190 Enzymes Human genes 0.000 claims description 36
- 108090000790 Enzymes Proteins 0.000 claims description 36
- 229940088598 enzyme Drugs 0.000 claims description 36
- 108091005804 Peptidases Proteins 0.000 claims description 28
- 239000004365 Protease Substances 0.000 claims description 24
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 24
- 239000003599 detergent Substances 0.000 claims description 23
- 239000004382 Amylase Substances 0.000 claims description 18
- 102000013142 Amylases Human genes 0.000 claims description 18
- 108010065511 Amylases Proteins 0.000 claims description 18
- 235000019418 amylase Nutrition 0.000 claims description 18
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 15
- 230000000694 effects Effects 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 7
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims description 7
- 102000004882 Lipase Human genes 0.000 claims description 6
- 108090001060 Lipase Proteins 0.000 claims description 6
- 239000004367 Lipase Substances 0.000 claims description 6
- 239000002253 acid Substances 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 6
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 6
- 239000000194 fatty acid Substances 0.000 claims description 6
- 229930195729 fatty acid Natural products 0.000 claims description 6
- 150000004665 fatty acids Chemical class 0.000 claims description 6
- 235000019421 lipase Nutrition 0.000 claims description 6
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical group C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 claims description 5
- 239000004280 Sodium formate Substances 0.000 claims description 5
- 230000002745 absorbent Effects 0.000 claims description 5
- 239000002250 absorbent Substances 0.000 claims description 5
- 125000004432 carbon atom Chemical group C* 0.000 claims description 5
- 150000002191 fatty alcohols Chemical class 0.000 claims description 5
- 125000004435 hydrogen atom Chemical group [H]* 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 239000003209 petroleum derivative Substances 0.000 claims description 5
- 150000003839 salts Chemical class 0.000 claims description 5
- HLBBKKJFGFRGMU-UHFFFAOYSA-M sodium formate Chemical compound [Na+].[O-]C=O HLBBKKJFGFRGMU-UHFFFAOYSA-M 0.000 claims description 5
- 235000019254 sodium formate Nutrition 0.000 claims description 5
- 230000000087 stabilizing effect Effects 0.000 claims description 5
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 4
- 102000004139 alpha-Amylases Human genes 0.000 claims description 4
- 108090000637 alpha-Amylases Proteins 0.000 claims description 4
- 239000001110 calcium chloride Substances 0.000 claims description 4
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 4
- 239000011888 foil Substances 0.000 claims description 4
- 230000002070 germicidal effect Effects 0.000 claims description 4
- 230000002209 hydrophobic effect Effects 0.000 claims description 4
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 4
- 229910052751 metal Inorganic materials 0.000 claims description 4
- 239000002184 metal Substances 0.000 claims description 4
- 239000011707 mineral Substances 0.000 claims description 4
- 235000015096 spirit Nutrition 0.000 claims description 4
- 239000004215 Carbon black (E152) Substances 0.000 claims description 3
- 229940024171 alpha-amylase Drugs 0.000 claims description 3
- 159000000007 calcium salts Chemical class 0.000 claims description 3
- 229930195733 hydrocarbon Natural products 0.000 claims description 3
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid group Chemical group C(CCCCCCC\C=C/CCCCCCCC)(=O)O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 claims description 3
- 229940057950 sodium laureth sulfate Drugs 0.000 claims description 3
- SXHLENDCVBIJFO-UHFFFAOYSA-M sodium;2-[2-(2-dodecoxyethoxy)ethoxy]ethyl sulfate Chemical compound [Na+].CCCCCCCCCCCCOCCOCCOCCOS([O-])(=O)=O SXHLENDCVBIJFO-UHFFFAOYSA-M 0.000 claims description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 2
- 150000001298 alcohols Chemical class 0.000 claims description 2
- 150000001768 cations Chemical class 0.000 claims description 2
- 125000001183 hydrocarbyl group Chemical group 0.000 claims description 2
- 239000011591 potassium Substances 0.000 claims description 2
- 229910052700 potassium Inorganic materials 0.000 claims description 2
- 239000011734 sodium Substances 0.000 claims description 2
- 229910052708 sodium Inorganic materials 0.000 claims description 2
- 238000003860 storage Methods 0.000 claims description 2
- 125000005233 alkylalcohol group Chemical group 0.000 claims 2
- 239000007864 aqueous solution Substances 0.000 claims 2
- 239000000243 solution Substances 0.000 claims 2
- 125000000129 anionic group Chemical group 0.000 claims 1
- 150000003138 primary alcohols Chemical class 0.000 claims 1
- 239000000203 mixture Substances 0.000 description 33
- 230000002255 enzymatic effect Effects 0.000 description 9
- 239000007788 liquid Substances 0.000 description 7
- 239000002736 nonionic surfactant Substances 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 229920002472 Starch Polymers 0.000 description 5
- 238000009472 formulation Methods 0.000 description 5
- 235000019698 starch Nutrition 0.000 description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 229920000742 Cotton Polymers 0.000 description 4
- 102000035195 Peptidases Human genes 0.000 description 4
- 239000003945 anionic surfactant Substances 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- 239000003381 stabilizer Substances 0.000 description 4
- 239000008107 starch Substances 0.000 description 4
- 239000004094 surface-active agent Substances 0.000 description 4
- 150000007513 acids Chemical class 0.000 description 3
- 238000004925 denaturation Methods 0.000 description 3
- 230000036425 denaturation Effects 0.000 description 3
- 235000013336 milk Nutrition 0.000 description 3
- 239000008267 milk Substances 0.000 description 3
- 210000004080 milk Anatomy 0.000 description 3
- 238000004806 packaging method and process Methods 0.000 description 3
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 2
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 2
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 2
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 2
- 239000005642 Oleic acid Substances 0.000 description 2
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 2
- -1 aromatic borate ester Chemical class 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 239000003925 fat Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 230000002459 sustained effect Effects 0.000 description 2
- DNIAPMSPPWPWGF-VKHMYHEASA-N (+)-propylene glycol Chemical compound C[C@H](O)CO DNIAPMSPPWPWGF-VKHMYHEASA-N 0.000 description 1
- YPFDHNVEDLHUCE-UHFFFAOYSA-N 1,3-propanediol Substances OCCCO YPFDHNVEDLHUCE-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 229920002261 Corn starch Polymers 0.000 description 1
- 241001125671 Eretmochelys imbricata Species 0.000 description 1
- 235000019759 Maize starch Nutrition 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- XBDQKXXYIPTUBI-UHFFFAOYSA-N Propionic acid Chemical class CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 1
- 244000299461 Theobroma cacao Species 0.000 description 1
- 235000009470 Theobroma cacao Nutrition 0.000 description 1
- 150000001242 acetic acid derivatives Chemical class 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- 125000004429 atom Chemical group 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 150000001734 carboxylic acid salts Chemical class 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000011121 hardwood Substances 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 125000003010 ionic group Chemical group 0.000 description 1
- 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
- 238000004900 laundering Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 238000005191 phase separation Methods 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 229920000166 polytrimethylene carbonate Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000003381 solubilizing effect Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000013112 stability test Methods 0.000 description 1
- 150000003890 succinate salts Chemical class 0.000 description 1
- 150000003871 sulfonates Chemical class 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 210000004916 vomit Anatomy 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
Landscapes
- Detergent Compositions (AREA)
Description
CT WO 0037602 PCT/US99/30883 . Pp)
A BLOOD AND ORGANIC STAIN REMOVER
The instant invention relates to the field of spot and stain removers for removing blood and organic stains. These blood and organic stain remover compositions contain at lcast two different types of enzymes and are stable with time as evidenced by sustained enzyme activity. The inventive spot and stain removers find use in medical offices, hospitals, and the like.
Blood and other organic stains such as for example urine, feces, vomit and the like, are notoriously difficult to remove from fabrics, such as for example clothing, and from hard surfaces, such as for example hardwood floors, cabinet counters, and dental instruments. Pre-spotting prior to the laundering of a garment is typically attempted using a liquid pre-spotting formulation or semi-solid stick formulation containing a detergent.
Pre-spotting with a detergent formulation alone has been at best only moderately successful at removing organic stains. Addition of an enzyme that is a protease can : potentially digest or at least partially digest the protein in the blood or organic stain.
However, enzyme containing liquid spot removers have known problems. Separation of : the different components of an aqueous spot remover containing an enzyme(s) into different layers or phases (termed phase separation) and a resulting decrease in spot removing capacity is known to occur.
Additionally, since enzymes themselves are proteins, they can digest each other.
Further, enzymes must maintain a certain chemical configuration or shape in order to remain active. A change to that configuration which results in inactivation is called denaturation. Heat, unfavorable pH, and other such conditions may result in denaturation or partial denaturation of the enzyme, thus decreasing the shelf-life of the spot remover or at least rendering the spot remover less effective with time. Formulating an effective organic spot remover containing enzymes that remains phase stable and that maintains enzymatic activity has proven difficult. Further, many spot removers contain phosphates and other chemicals that can pollute the environment.
Various patents have proposed ways to stabilize single enzyme type detergent compositions. United States Patent No. 3,819,528 provides an aqueous enzyme composition wherein the enzyme is an amylase. A water soluble calcium salt, and an
Pa organic stabilizing agent such as 1,3-propanediol or an aliphatic glycol are illustrated as stabilizers. United States Patent No. 4.287,082 (Tolfo, et al), incorporated herein by reference, discloses a homogeneous proteolytic enzyme-containing liquid detergent composition containing saturatured fatty acids. U.S. Patent No. 5,422,030, the disclosure of which is incorporated herein by reference, provides liquid detergents with an aromatic borate ester to inhibit proteolytic enzyme degradation of the enzymes in the composition.
It is an object of the instant invention to provide a liquid spot or pre-spot remover solution containing detergent and at least two enzymes that has enhanced enzymatic stability and sustained organic and blood spot removing capacity.
It is an additional object to provide a blood and organic spot remover that is environmentally friendly and that is biodegradable.
It is a further object of the instant invention to provide a liquid spot or pre-spot remover containing detergent and at least one protease enzyme where the activity of the protease enzyme is at lcast 80%, and preferably 90% or greater, for at least eight weeks.
It is a still further object of the instant invention to provide a spot or pre-spot : remover having an enzyme system comprising at least two enzymes, a protease and an alpha amylase and a detergent that maintains activity or enzymatic stability for at least - eight weeks. Enzymatic stability is defined as the ability of an enzyme to maintain its catalytic activity to a designated substrate in order to digest the substrate into shortened components that are more easily washed away in detergent and/or with water. Preferably the spot remover composition as a whole is phase stable and active for at least eight weeks.
It is an additional object of the present invention to provide a blood and organic spot remover that has germicidal activity.
The instant invention is a blood and organic stain remover comprising a proteasc, an amylase, an enzyme stabilizing system, a nonionic detergent, and water. A method of using the aqueous blood and organic stain remover composition as a germicide is also provided. The composition may be incorporated into a pre-moistened prepackaged wiper that allows the user to remove a blood and organic stain without coming into direct contact with the stain. The packaging folds so that the moistened area of the wiper need not come in contact with the skin of the user. The wiper is especially useful for cleaning clothing and hard surfaces such as for example, tables, chairs, instruments and the like.
©. WO0037602 PCT/US99/30883 ‘ >
Figure 1 illustrates the ability of Formula A and Formula B to remove stains containing protein therein as measured by reflectance.
Figure 2 illustrates the ability of Formula A and Formula B to remove stains having starch therein as measured by reflectance.
Figure 3A illustrates the stability of Formula A with time as determined by the enzymatic activity therein.
Figure 3B illustrates the stability of Formula B with time as determined by the enzymatic activity therein.
Figure 4 is a pictorial representation of the prepackaged blood and organic stain remover of this invention.
In accordance with the present invention, it has been found that by combining a nonionic surfactant or a mixture of nonionic and anionic surfactants, a protease or a - mixture of a protease and an amylase, and a stabilizing system, effective organic spot removing enzymatic activity in an aqueous detergent solution may be maintained for : prolonged periods of time.
In the inventive organic stain remover, water comprises up to 50% by weight of the total composition. The amount of water present will vary depending upon the other solvents needed to dissolve certain components of the formulation(s) and/or to clarify the liquid composition. The preferred amount of water is from about 40% to about 60%.
Organic Synthetic Surface-Active Agents: i
The preferred organic synthetic surface-active agents are biodegradable and are nonionic surfactants, anioinic surfactants, or mixtures of nonionic and anionic surfactants.
The nonionic surfactants that are suitable are ethoxylated fatty alcohols and ethoxylated fatty acids. Sulfonates and succinates may also be useful. Ethoxylates are produced by condensing ethylene oxide with a hydrocarbon having a reactive hydrogen atom in the presence of an acid or base catalyst. Such surfactants can be represented by the generic formula RA(CHy CH0),,H wherein R represents the hydrophobic moiety, A represents the group carrying the reactive hydrogen atom and n represents the group average number of ethylene oxide moieties. R typically contains about 8 to 22 carbon
# "atoms, and n usually varies from about 2 to about 10, and preferably from about 2 to about 8 groups/mole, avg. The hydrophile/lipophile balance number (HLB) is preferably between about 3 to about 15. Preferred ethoxylated fatty alcohols nonionic surfactants include NeodolR 23-6.5 and NeodolR 25-3 (each available from Shell Chemical Co.;
Parsippany, NJ).
Anionic surfactants can be represented by the general formula RSOM wherein R represents a hydrocarbon selected from the group consisting of straight or branched alkyl radicals containing from about 8 to about 24 carbon atoms. M is a salt forming cation that typically is selected from the group consisting of sodium, potassium, ammonium and derivatives thereof. The preferred anionic surfactant is a simple salt such as for example, sodium laureth sulfate (available from Stepan Co., Northfield, IL).
The Enzymes:
Enyzmes that are useful in the present invention comprise proteases and alpha- amylases. The proteases are typically derived from bacterial sources and are preferably active in a pH range of about 4 to about 10 The preferred pH range is neutral to somewhat basic, from about a pH of 6.8 to about a pH of 9. A protease can break the peptide bonds of proteins found in blood and organic stains. This results in short chain molecules having : free amino and carboxy groups, ionic groups that are more easily washed away with water and/or detergent. It is preferred that the protease be able to function at high temperatures } (up to about 90 degrees C) as well as at room temperature. An example of such a protease is SavinaseR 16L which is available from Novo Nordisk BioChem North America, Inc.,
Franklinton, NC.
An amylase is an enzyme that catalyzes a reaction that breaks starch molecules into shorter chain molecules making the removal of the starch with water and/or detergent easier. Starches may enhance the adherence of proteins to fibers and hard surfaces. The preferred amylase is able to function at high temperatures (up to about 90 degrees C) as well as at room temperature. Preferably, the amylase can function at a pH from about 6.8 to about 11.5 An example of an amylase is TermamyIR 300L which is available from
Novo Nordisk BioChem North America, Inc., Franklinton, NC.
Optionally, an enzyme that facilitates the breakdown of fats and oils into shorter chain molecules may be added to the spot remover. Such an enzyme is a lipase such as for example, LipolaseR which is available from Novo Nordisk BioChem North America, Inc.,
: ©. WO 0037602 PCT/US99/30883 ’ J
Franklinton, NC. Fats and oils may enhance adherence of other organic molecules. By combining a lipase, a protease, and optionally, an amylase in an aqueous detergent solution, the types of stains removed may be uncxpectedly improved.
In the preferred blood and organic spot remover composition, at an amylase and a protease are present at a concentration of at least about 0.1% w/w.
The Enzyme Activity Stabilizers:
Useful enzyme activity stabilizers include compounds that provide a source of free calcium in the solution such as for example calcium salts; alkyl or branched alcohols such as for example ethanol and isopropyl! alcohol; alkanolamines such as for example triethanolamine and acids preferably organic acids; and mixtures of petroleum distillates.
Up to 25% by weight of alcohol may be added to the spot remover. Preferably between 1 — 15% by weight of alcohol are added. Petroleum distillates such as for example mineral spirits may be added in amounts of up to 75% by weight with less than 25% being preferred.
The pH of the spot remover may be adjusted by adding an acid or a base to achieve the preferred pH which is from about 6.8 to 8.5. Alkanolamines such as for example : triethanolamine, may be added to make the solution more basic. Acids or salts of acids may be added to adjust the pH towards a lower pH. Useful carboxylic acid salt includes : acetates and propionates. The preferred salt is sodium formate.
Unsaturated fatty acids may also be added such as for example oleic acid. Such fatty acids may serve a dual function by enhancing cleaning power and altering pH.
To obtain the spot remover solution all ingredients are dissolved and mixed together. The final volume is adjusted with distilled water (DI).
The present invention is useful for cleaning up fresh and dried blood and organic _ stains and spots when packaged as a wiper, such as for example in a dentist's office.
Alternatively, direct skin contact with the inventive composition and spot may be prevented by use of gloves by the user. The present invention may also find use as a germicide, such as when wiping up blood and organic stains. The inventive compositions also find use as pre-spotting compositions. For example, a spot or stain on a laboratory coat may be treated with the chosen inventive composition, then the laboratory coat is washed in the regular manner.
The present inventive composition(s) may be incorporated into prepackaged disposable wipers 10, for example such as illustrated in United States Patent No. 4,998,984
J
(McClendon), the disclosure of which is incorporated herein by reference. The package may have an absorbent material wipe 12 attached to and enclosed in a metal foil package 14 provided with fold back flaps 16. The inventive composition selected from Formulae A or B is placed on the absorbent material prior to packaging. The fold-back flaps may be used to form a handle 18 for the wipe to clean up blood or spot an organic stain. After contacting the spot with the wipe and removing the spot, the flaps may be folded over the used wipe without the need for direct skin contact with the wipe. Alternate methods of packaging the invention composition which allow use without the need for direct skin contact with the blood or organic spot or stain or the inventive composition are contemplated. Additionally, the user may remove spots or stains with the invention compositions by protecting the skin through use of gloves.
The following examples illustrate the invention:
Example 1
The following illustrate the compositions of the present invention. Preparation of the following Formulae is achieved by solubilizing, clarifying and mixing the components.
All parts, percentages and ratios used herein are by weight unless otherwise specified.
Formula A Formula B calcium chloride 0.05% NeodolR 25-3 10% sodium formate 2% NeodolR 23-6.5 10% triethanolamine 1% mineral spirits 20% ethanol 5% isopropanol 12%
NeodolR 23-6.5 30% triethanolamine about 1.2%
SteolR CS-460 20% oleic acid about 2.3% enzymes 0.2-3% calcium chloride 0.05%
DI water to 100% sodium formate 2% enzymes 0.2-3%
DI water to 100%
Enzymes: SavinaseR 16L, LipolaseR 100L, and TermamyIR 300L.
Example 2
The effectivencss of Formula A and Formula B at removing organic stains was tested with and without added enzymes on standardized swatches having the following
©. wb 00/37602 PCT/US99/30883 : s compositions: I - blood. milk, carbon ink on 100% cotton (100%); II — blood, milk, carbon ink on polyester-cotton; III — cocoa and milk on 100% cotton; IV — maize starch and carbon ink on 100% cotton. Swatches I and II were observed for protease activity.
Swatches III and IV were observed for amylase activity. . LipolaseR 100L was observed to separate out of each of Formulae A and B.
On a weight/weight basis, equal amounts of each of SavinaseR 16L, LipolaseR 100L, and TermamylR 300L were tested in Formulae A and B at concentrations ranging from 0 to 0.5%. One milliliter aliquots of the respective Formulae were rubbed for 10 seconds into the stain on each of the standardized swatches. The standardized swatches were allowed to soak for 5 minutes and then were rinsed in tap water for 5 minutes. A refractometer (US Testing Co., Model 7243S) was used to provide reflectance measurements.
The results shown in Fig. 1 for protease activity and in Fig. 2 for protease/amylasc activity, demonstrate that while the detergent composition alone was effective at removing organic stains, addition of the protease and the amylase greatly enhanced organic stain removal even at concentrations as low as 0.1% w/w.
Example 3
Storage stability of Formulae A and B when 1% each of SavinaseR 16L, LipolaseR 100L, and TermamylR 300L were present in the aqueous detergent composition was determined using a standard accelerated stability test. An eight week incubation at an elevated temperature of 30 degrees C was performed wherein enzymatic activity was determined at two week intervals. Each formulation was incubated in sealed glass vials in an incubation chamber (VWR Scientific Laboratories, Inc., Model #535). Enzymatic activity of the protease, amylase, and lipase was determined using a spectrophotometer (Carey, Inc., Model 3C) and protein or starch standards. Wash performance evaluation was performed using a terg-o-tometer (United States Testing Co., Inc.; 7243S). Results of the wash test were evaluated using a Hunter Labscan reflectometer (SN/13859). Figure 3A illustrates protease stability with time in Formulae A and B. Figure 3B illustrates amylase activity with time in Formulae A and B. It can be seen that both enzymes in both formulae retain greater than 90% activity with time.
The foregoing is considered only illustrative of the currently preferred embodiments of the invention presented herein. Since numerous modifications and
Lo he & . . N
Pa changes will occur to those skilled in the art, it is not desired to limit the invention to the exact construction used to illustrate the various means comprising this invention.
What is claimed is:
Claims (27)
1. A prepackaged blood and organic spot remover comprising a protease, an amylase, an enzyme stabilizing system, a detergent, and water, said prepackaged blood and organic spot remover being contained in a prepackaged disposable wiper having an absorbent material wipe attached thereto that is soaked with said blood and organic spot remover, said prepackaged disposable wiper being enclosed in a metal foil package provided with fold back flaps which can be used to form a handle for said prepackaged disposable wiper while cleaning up blood or an organic stain and which can be folded over said prepackaged disposable wiper once used.
2. A prepackaged blood and organic spot remover comprising an aqueous solution of 0.05% calcium chloride; 2% sodium formate; 1% triethanolamine; 5% ethanol; 30% linear primary alcohol ethoxylate selected from the group consisting of ethoxylated fatty alcohols and ethoxylated fatty acids represented by the generic formula RA(CH, CH»0O),,H wherein R represents the hydrophobic moiety and has between 8 and 22 carbon atoms, A represents the group carrying the reactive hydrogen atom, and n represents the group average number of ethylene oxide moieties which is between 2 and 8; 20% sodium laureth sulfate; and an enzyme solution having a concentration of between 0.2% and 3% by weight wherein the enzymes present are protease, lipase, and alpha-amylase; said prepackaged blood and organic spot remover being contained in a prepackaged disposable wiper having an absorbent material wipe attached thereto that is soaked with said blood and organic spot remover, said prepackaged disposable wiper being enclosed in a metal foil package provided with fold back flaps which can be used to form a handle for said prepackaged disposable wiper while cleaning up blood or an organic stain and which can be folded over said prepackaged disposable wiper once used.
3. A prepackaged blood and organic spot remover comprising an aqueous solution of 20% linear alcohol ethoxylate selected from the group consisting of ethoxylated fatty alcohols and ethoxylated fatty acids represented by the generic formula RA(CH, CH50O),H wherein R represents the hydrophobic moiety and has between 8 and 22 carbon atoms, A represents the group carrying the reactive hydrogen atom, and n represents the group average number of ethylene oxide moieties which is between 2 and 8; 20% mineral spirits; 12% isopropanol; about 1.2% triethanolamine; 2.3% oleic Amended 11 October 2002 acid; 0.05% calcium chloride; 2% sodium formate; and an enzyme solution having a concentration of between 0.2% and 3% by weight wherein the enzymes present are protease, lipase, and alpha- amylase; said prepackaged blood and organic spot remover being contained in a prepackaged disposable wiper having an absorbent material wipe attached thereto that is soaked with said blood and organic spot remover, said prepackaged disposable wiper being enclosed in a metal foil package provided with fold back flaps which can be used to form a handle for said prepackaged disposable wiper while cleaning up blood or an organic stain and which can be folded over said prepackaged disposable wiper once used.
4. The blood and organic spot remover of claim 1 wherein said blood and organic spot remover has germicidal properties.
5. The blood and organic spot remover of claim 1 wherein said blood and organic spot remover is biodegradable.
6. The blood and organic spot remover of claim 1 wherein the activity range of the protease is between pH 6.8 and 9.
7. The blood and organic spot remover of claim 1 wherein the protease will function at temperatures of about 90 degrees C.
8. The blood and organic spot remover of claim | wherein the activity range of the amylase is between pH 6.8 and 11.5.
9. The blood and organic spot remover of claim 1 wherein the amylase will function at temperatures of about 90 degrees C.
10. The blood and organic spot remover of claim 1 wherein lipase is included.
11. The blood and organic spot remover of claim 1 wherein the combined concentration of the protease and the amylase is 0.1% by weight.
12. The blood and organic spot remover of claim 1 wherein the activity of the protease and the amylase is between 80% and 90% after 8 weeks of storage.
13. The blood and organic spot remover of claim 1 wherein the enzyme stabilizing system includes an alkanolamine, a calcium salt, and an alcohol. Amended 11 October 2002 i . SA © WO 00/37602 PCT/US99/30883
14. The blood and organic spot remover of claim 13 wherein the alkanolamine is triethanolamine.
15. The blood and organic spot remover of claim 13 wherein the alcohol is selected from the group consisting of alkyl alcohols and branched alcohols.
16. The blood and organic spot remover of claim 15 wherein the alkyl alcohol is selected from the group consisting of ethanol and isopropyl alcohol.
17. The blood and organic spot remover of claim 13 wherein the alcohol is present at concentrations of up to 25% by weight.
18. The blood and organic spot remover of claim 1 wherein the enzyme stabilizing system further includes a petroleum distillate.
19. The blood and organic spot remover of claim 18 wherein the petroleum distillate is mineral spirits.
20. The blood and organic spot remover of claim 18 wherein the petroleum distillate is present at concentrations of up to 75% by weight.
21. The blood and organic spot remover of claim 1 wherein the detergent is nonionic.
22. The blood and organic spot remover of claim 21 wherein the hydrophile/lipophile balance number of the nonionic detergent is between about 3 and about 15.
23. The blood and organic spot remover of claim 21 wherein the nonionic detergent is selected from the group consisting of ethoxylated fatty alcohols and ethoxylated fatty acids represented by the generic formula RA(CHy CHO), H wherein R represents the hydrophobic E moiety and has between 8 and 22 carbon atoms, A represents the group carrying the reactive hydrogen atom, and n represents the group average number of ethylene oxide moieties which is between 2 and 8.
24. The blood and organic spot remover of claim 1 wherein the detergent is anionic.
25. The blood and organic spot remover of claim 24 wherein the anionic detergent is represented by the general formula RSOM wherein R represents a hydrocarbon selected from the group consisting of straight or branched alkyl radicals containing from about 8 to about 24 Amended 11 October 2002
/ . © Co WO 00/37602 PCT/US99/30883 carbon atoms, M is a salt forming cation which is selected from the group consisting of sodium, potassium, ammonium and derivatives thereof.
26. The blood and organic spot remover of claim 24 wherein the anionic detergent is sodium laureth sulfate.
27. The blood and organic spot remover of claim 1 wherein the water comprises between 40% and 50% of total weight. Amended 11 October 2002
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11383098P | 1998-12-23 | 1998-12-23 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ZA200106034B true ZA200106034B (en) | 2002-07-23 |
Family
ID=22351754
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ZA200106034A ZA200106034B (en) | 1998-12-23 | 2001-07-23 | A blood and organic stain remover. |
Country Status (2)
| Country | Link |
|---|---|
| AU (1) | AU2845600A (en) |
| ZA (1) | ZA200106034B (en) |
-
1999
- 1999-12-23 AU AU28456/00A patent/AU2845600A/en not_active Abandoned
-
2001
- 2001-07-23 ZA ZA200106034A patent/ZA200106034B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| AU2845600A (en) | 2000-07-12 |
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