YU47795A - Metod za ekstra-celularnu ekspresiju proteina - Google Patents

Metod za ekstra-celularnu ekspresiju proteina

Info

Publication number
YU47795A
YU47795A YU47795A YU47795A YU47795A YU 47795 A YU47795 A YU 47795A YU 47795 A YU47795 A YU 47795A YU 47795 A YU47795 A YU 47795A YU 47795 A YU47795 A YU 47795A
Authority
YU
Yugoslavia
Prior art keywords
extra
proteins
cellular
protein expression
dna sequence
Prior art date
Application number
YU47795A
Other languages
English (en)
Inventor
W.S. Queener
M.J. Zock
Original Assignee
Eli Lilly And Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly And Company filed Critical Eli Lilly And Company
Publication of YU47795A publication Critical patent/YU47795A/sh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1014—Hydroxymethyl-, formyl-transferases (2.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Diabetes (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

METOD ZA EKSTRA-CELULARNU EKSPRESIJU PROTEINA - Heterologna ekstra-celularna ekspresija rekombinantnih proteina u rastvornom obiiku je poželjna zbog lakog prečišćavanja izlučenog proteina i izbegavanja potrebe za ekstrakcijom iz ćelije i procedure dovođenja u odgovarajuću konfiguraciju. Ova istrazivanja obezbeđuju DNA sekvencu prirodnog gena ftalil amidaze izolovane iz bakterije Xan-thobacter agilis koja kontroliše transkripciju, translaciju i ekstra-celularno izlučivanje proteina u Streptomyces lividans. Ova DNA sekvenca se može koristiti u metodi za ekstra-celularnu ekspresiju velikog broja proteina u rastvornom funkcionalnom obliku.
YU47795A 1994-07-15 1995-07-14 Metod za ekstra-celularnu ekspresiju proteina YU47795A (sh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/275,487 US5658755A (en) 1994-07-15 1994-07-15 Method for extra-cellular expression of protein

Publications (1)

Publication Number Publication Date
YU47795A true YU47795A (sh) 1997-12-05

Family

ID=23052510

Family Applications (1)

Application Number Title Priority Date Filing Date
YU47795A YU47795A (sh) 1994-07-15 1995-07-14 Metod za ekstra-celularnu ekspresiju proteina

Country Status (8)

Country Link
US (1) US5658755A (sh)
AU (1) AU3098195A (sh)
CO (1) CO4410178A1 (sh)
IL (1) IL114594A0 (sh)
PE (1) PE34296A1 (sh)
WO (1) WO1996002637A1 (sh)
YU (1) YU47795A (sh)
ZA (1) ZA955900B (sh)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0807104A2 (en) * 1995-02-02 1997-11-19 Smithkline Beecham Plc Heterocyclic compounds possessing 5ht 2c? receptor antagonist activity
KR100285950B1 (ko) * 1997-12-26 2001-04-16 윤종용 인체의영향을최소화시키는휴대용전화기의안테나회로를구현하는방법
KR100312456B1 (ko) 1999-03-13 2001-11-03 윤덕용 슈도모나스 플루오레슨스 유래의 외래단백질 분비촉진유전자
DE19915938A1 (de) 1999-04-09 2000-10-19 Aventis Pharma Gmbh Herstellung von pankreatischer Procarboxypeptidase B, Isoformen und Muteinen davon und ihre Verwendung
CN113215185B (zh) * 2021-04-22 2022-04-29 浙江工业大学 一种用于重组表达棘白菌素b脱酰基酶的重组基因序列
CN113174398B (zh) * 2021-04-22 2022-04-29 浙江工业大学 一种用于重组表达棘白菌素b脱酰基酶的表达盒及应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5032510A (en) * 1984-09-26 1991-07-16 Eli Lilly And Company Method for expression and secretion in bacillus
US5200327A (en) * 1985-11-06 1993-04-06 Cangene Corporation Expression system for the secretion of bioactive human granulocyte macrophage colony stimulating factor (GM-CSF) and other heterologous proteins from streptomyces
US4977089A (en) * 1987-01-30 1990-12-11 Eli Lilly And Company Vector comprising signal peptide-encoding DNA for use in Bacillus and other microorganisms
US5451522A (en) * 1994-07-15 1995-09-19 Eli Lilly And Company Genes encoding and method of expressing an enzyme: phthalyl amidase

Also Published As

Publication number Publication date
IL114594A0 (en) 1995-11-27
PE34296A1 (es) 1996-09-04
AU3098195A (en) 1996-02-16
WO1996002637A1 (en) 1996-02-01
US5658755A (en) 1997-08-19
CO4410178A1 (es) 1997-01-09
ZA955900B (en) 1997-01-14

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