ES2184567A1 - Procedimiento para la solubilizacion de proteinas. - Google Patents

Procedimiento para la solubilizacion de proteinas.

Info

Publication number
ES2184567A1
ES2184567A1 ES200001935A ES200001935A ES2184567A1 ES 2184567 A1 ES2184567 A1 ES 2184567A1 ES 200001935 A ES200001935 A ES 200001935A ES 200001935 A ES200001935 A ES 200001935A ES 2184567 A1 ES2184567 A1 ES 2184567A1
Authority
ES
Spain
Prior art keywords
inclusions
solubilisation
proteins
incubation
host cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES200001935A
Other languages
English (en)
Other versions
ES2184567B1 (es
Inventor
Maria Del Mar Carrio
Antonio Villaverde
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universitat Autonoma de Barcelona UAB
Original Assignee
Universitat Autonoma de Barcelona UAB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universitat Autonoma de Barcelona UAB filed Critical Universitat Autonoma de Barcelona UAB
Priority to ES200001935A priority Critical patent/ES2184567B1/es
Publication of ES2184567A1 publication Critical patent/ES2184567A1/es
Application granted granted Critical
Publication of ES2184567B1 publication Critical patent/ES2184567B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Procedimiento para la solubilización de proteínas. El procedimiento de la presente invención tiene por objeto la purificación de proteínas solubles y biológicamente activas, y consiste esencialmente en la incubación de cuerpos de inclusión que contienen la proteína de interés con un extracto celular purificado. El procedimiento para la solubilización de proteínas de la presente invención comprende las etapas de: a) cultivo de células huésped que expresan la proteína de interés en forma de cuerpos de inclusión; b) lisado de dichas células huésped y extracción y purificación de los cuerpos de inclusión; y se caracteriza por el hecho de que comprende además: c) obtención de un extracto celular purificado; d) tratamiento de los cuerpos de inclusión con dicho extracto celular purificado a una temperatura adecuada; y e) aislamiento y purificación de la proteína solubilizada.
ES200001935A 2000-07-31 2000-07-31 Procedimiento para la solubilizacion de proteinas. Expired - Fee Related ES2184567B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES200001935A ES2184567B1 (es) 2000-07-31 2000-07-31 Procedimiento para la solubilizacion de proteinas.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200001935A ES2184567B1 (es) 2000-07-31 2000-07-31 Procedimiento para la solubilizacion de proteinas.

Publications (2)

Publication Number Publication Date
ES2184567A1 true ES2184567A1 (es) 2003-04-01
ES2184567B1 ES2184567B1 (es) 2004-09-16

Family

ID=8494520

Family Applications (1)

Application Number Title Priority Date Filing Date
ES200001935A Expired - Fee Related ES2184567B1 (es) 2000-07-31 2000-07-31 Procedimiento para la solubilizacion de proteinas.

Country Status (1)

Country Link
ES (1) ES2184567B1 (es)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993025681A1 (en) * 1992-06-11 1993-12-23 New York University A cytoplasmic chaperonin and methods of making and using it
EP0743365A2 (en) * 1995-05-17 1996-11-20 Ajinomoto Co., Inc. Process for efficiently producing transglutaminase through DNA recombination

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993025681A1 (en) * 1992-06-11 1993-12-23 New York University A cytoplasmic chaperonin and methods of making and using it
EP0743365A2 (en) * 1995-05-17 1996-11-20 Ajinomoto Co., Inc. Process for efficiently producing transglutaminase through DNA recombination

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CARRIË, M.M. et al. "Proteolytic digestion of bacterial inclusion body proteins during dynamic transition between soluble and insoluble forms". BIOCHIMICA ET BIOPHYSICA ACTA. Vol. 1434, nö 1, 1999, páginas 170-1976, todo el documento. *

Also Published As

Publication number Publication date
ES2184567B1 (es) 2004-09-16

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