WO2024135159A1 - 試料処理システム - Google Patents

試料処理システム Download PDF

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Publication number
WO2024135159A1
WO2024135159A1 PCT/JP2023/040826 JP2023040826W WO2024135159A1 WO 2024135159 A1 WO2024135159 A1 WO 2024135159A1 JP 2023040826 W JP2023040826 W JP 2023040826W WO 2024135159 A1 WO2024135159 A1 WO 2024135159A1
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WO
WIPO (PCT)
Prior art keywords
unit
slide glass
staining
processing system
section
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2023/040826
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English (en)
French (fr)
Japanese (ja)
Inventor
茂樹 松原
隆之 小原
英利 杉山
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hitachi High Tech Corp
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Hitachi High Tech Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi High Tech Corp filed Critical Hitachi High Tech Corp
Priority to JP2024565671A priority Critical patent/JPWO2024135159A1/ja
Priority to EP23906530.3A priority patent/EP4641211A1/en
Priority to CN202380086546.0A priority patent/CN120359402A/zh
Publication of WO2024135159A1 publication Critical patent/WO2024135159A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G01N1/312—Apparatus therefor for samples mounted on planar substrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4022—Concentrating samples by thermal techniques; Phase changes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/00722—Communications; Identification
    • G01N35/00732—Identification of carriers, materials or components in automatic analysers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/0092—Scheduling
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4022—Concentrating samples by thermal techniques; Phase changes
    • G01N2001/4027—Concentrating samples by thermal techniques; Phase changes evaporation leaving a concentrated sample
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00039—Transport arrangements specific to flat sample substrates, e.g. pusher blade
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099—Characterised by type of test elements
    • G01N2035/00138—Slides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/00722—Communications; Identification
    • G01N35/00732—Identification of carriers, materials or components in automatic analysers
    • G01N2035/00821—Identification of carriers, materials or components in automatic analysers nature of coded information
    • G01N2035/00851—Identification of carriers, materials or components in automatic analysers nature of coded information process control parameters

Definitions

  • the present invention relates to a sample processing system, and in particular to a sample processing system capable of automatically processing biological samples.
  • tissue or blood are processed to create a slide for microscopic observation, and the tissue on the slide is stained and observed under a microscope.
  • Patent Document 1 JP Patent Publication No. 2012-141287 proposes an automatic processing system.
  • Patent Document 1 discloses the modules with what functions the automatic processing system is made up of, it does not disclose how the individual processing modules should be specifically arranged or how the slide glasses should be handled in order to be transported between each processing module.
  • the object of the present invention is to provide a biological processing system for pathological testing that is composed of multiple processing modules, in which each processing module is optimally positioned and the entire processing can be performed efficiently.
  • the sample processing system has an input/recovery section including an input mechanism for storing a plurality of slide glasses on which samples are placed before staining, dehydration, mounting, or scanning, and a recovery mechanism for storing the slide glasses after processing, a staining section for performing the staining process on the samples placed on the slide glasses, a post-processing section for performing the dehydration process, mounting, or scanning process on the samples placed on the slide glasses, and a transport section capable of transporting the slide glasses between the staining section and the input/recovery section, and between the input/recovery section and the post-processing section, and is arranged in the order of the staining section, the input/recovery section, and the post-processing section.
  • a sample processing system capable of performing processing efficiently is provided.
  • FIG. 1A and 1B are a plan view and a block diagram showing an overview of a sample processing system according to an embodiment.
  • 1 is a side view showing an overview of a sample processing system according to an embodiment.
  • FIG. 2 is a plan view showing a tray on which slide glasses are placed in the embodiment.
  • FIG. 2 is a plan view showing a slide glass transport unit in the embodiment.
  • FIG. 2 is a perspective view showing a slide glass transport mechanism according to the embodiment.
  • FIG. 2 is a perspective view showing a slide glass insertion and recovery section in the embodiment.
  • 2 is a perspective view showing an example of a slide glass input/output section in an embodiment;
  • FIG. 10 is a flowchart showing a slide glass transport process according to the embodiment. 10 is a flowchart specifically illustrating a slide glass transport process in the embodiment.
  • the X, Y, and Z directions described in this application intersect and are perpendicular to each other.
  • the X1 direction of the X directions is the right direction
  • the X2 direction of the X directions is the left direction
  • the Y1 direction of the Y directions is the forward direction
  • the Y2 direction of the Y directions is the backward direction
  • the Z1 direction of the Z directions is the upward direction
  • the Z2 direction of the Z directions is the downward direction.
  • a sample processing system 100 according to this embodiment will be described below with reference to Figures 1 to 8.
  • a sample is placed on a slide glass 1 used in this embodiment.
  • the sample is, for example, a sample used in a pathological examination, and is a biological sample such as tissue or blood.
  • the sample processing system 100 constitutes a part of an examination device such as a pathological staining device.
  • FIG. 1 is a schematic plan view and block diagram showing the sample processing system of this embodiment.
  • FIG. 2 is a schematic side view showing the sample processing system of this embodiment.
  • FIG. 2 is a side view of the structure of FIG. 1 as seen from below in FIG. 1, that is, a side view of the structure of FIG. 1 as seen in the Y2 direction.
  • the components that make up the sample processing system are thought to be sealed in a container, but here the internal structure is shown through the sealing container.
  • the slide glass 1 is hatched to make the drawing easier to understand.
  • the sample processing system 100 includes a loading/recovery section (loading/storing module) 10, a plurality of transport mechanisms (gripper units) 30, a staining section (staining module) 40, a dehydration section (dehydration module) 51, an encapsulation section (encapsulation module) 52, a scanning section (scanning module) 53, and a transport section (transport line) 60.
  • the dehydration section 51, the encapsulation section 52, and the scanning section 53 make up a post-processing section 50.
  • the staining section 40 includes a disk and a reagent supply section, and is provided to perform a staining process on a sample placed on the slide glass 1.
  • the slide glass 1 is placed on the disk in the staining section 40, and the sample placed on the slide glass 1 is stained with a reagent supplied from the reagent supply section.
  • the staining method used in the staining section 40 can be, for example, hematoxylin-eosin (HE) staining, immunohistochemistry (IHC) staining, or in-situ hybridization (ISH) staining.
  • the sample processing system 100 is exemplified as having two staining units 40 arranged side by side.
  • the number of staining units 40 arranged on the right side (X1 direction side) of the input/recovery unit 10 may be one or three or more.
  • each of the multiple staining units 40 arranged side by side may perform staining processing using a different staining method.
  • the loading/recovery section 10 includes a loading mechanism 11, a recovery mechanism 12, and multiple trays (slide trays) 13.
  • the loading mechanism 11 is provided for storing the slide glasses 1 before staining, dehydration, mounting, or imaging (imaging, scanning) processing is performed.
  • the recovery mechanism 12 is provided for storing the slide glasses 1 after imaging processing has been performed.
  • the feeding mechanism 11 and the recovery mechanism 12 each have trays 13 arranged in multiple layers stacked in the vertical direction (Z direction).
  • the multiple trays 13 stacked in the Z direction can be moved in the Y1 and Y2 directions by a moving mechanism provided in the feeding and recovery unit 10.
  • a plurality of slide glasses 1 are placed on the tray 13.
  • the tray 13 has a gripping portion (handle) at the end in the Y1 direction.
  • each slide glass 1 is placed on one placement table on the tray 13.
  • a label or the like is attached to each slide glass 1, and the label has an identification code 1a indicating information about each sample.
  • the loading and recovering section 10 is provided with an identification code reader (reading section), and the information contained in the identification code 1a of each slide glass 1 is read by the identification code reader.
  • a barcode, a two-dimensional code, or an RFID (Radio Frequency Identification) can be used as the identification code 1a.
  • the identification code 1a is not limited to these, and other types of identifiers may be used as long as they can hold information about the slide glass 1.
  • the dehydration unit 51 is provided to perform a process (dehydration process) of replacing the water contained in the sample with an organic solvent (e.g., xylene) so that the slide glass 1 can be preserved. In other words, the dehydration unit 51 performs a dehydration process on the sample placed on the slide glass 1.
  • an organic solvent e.g., xylene
  • the encapsulation section 52 is a cover slip encapsulation unit.
  • the slide glass 1 that has been subjected to the staining process and the dehydration process is transported to the encapsulation section 52.
  • a cover slip (cover glass) is placed (encapsulation process) on the slide glass 1 so as to cover the stained sample.
  • the sample placed on the slide glass 1 is encapsulated.
  • the scanning unit 53 is provided to observe or photograph (image) the stained sample on the slide glass 1 that has been subjected to the staining, dehydration and mounting processes under a microscope. That is, the scanning unit 53 performs observation processing, which includes observing the sample under a microscope, obtaining a digital image using a slide scanner and observing the sample using the image, or both. In this application, observing the sample under a microscope and photographing the sample are collectively referred to as scanning (scanning processing). That is, the scanning unit 53 performs scanning processing on the sample placed on the slide glass 1.
  • the sample processing system 100 is equipped with a transport section 60 for transporting the slide glass 1.
  • the transport section 60 has a first transport line 61 and a second transport line 62.
  • the first transport line 61 and the second transport line 62 extend in the X direction along the staining section 40, the loading and recovery section 10, and the post-processing section 50.
  • a carrier 20 is mounted on each of the first transport line 61 and the second transport line 62.
  • the carriers 20 of the first transport line 61 and the second transport line 62 move (slide) in the Y direction to transport the slide glass 1 placed on the carrier 20.
  • the first transport line 61 is used when transporting the slide glass 1 before staining processing from the input mechanism 11 to the staining section 40, or when transporting the slide glass 1 after scanning processing from the scanning section 53 to the recovery mechanism 12. In other words, the first transport line 61 transports the slide glass 1 only in the right direction (X1 direction) in Figures 1 and 4.
  • the second conveying line 62 is used to convey the slide glass 1 after the staining process from the staining section 40 to the dehydration section 51 of the post-processing section 50.
  • the second conveying line 62 conveys the slide glass 1 only in the left direction (X2 direction) in Figures 1 and 4.
  • a plurality of guide pins 23 are provided on the upper surface of the carrier 20.
  • the first conveying line 61 is provided with a guide 61a
  • the second conveying line 62 is provided with a guide 62a.
  • the first conveying line 61 and the second conveying line 62 are each provided with an actuator 64.
  • the transport unit 60 can transport the slide glass 1 between the staining unit 40 and the loading and recovery unit 10, and between the loading and recovery unit 10 and the post-processing unit 50.
  • the carrier 20 on which the slide glass 1 is placed can be moved in the X1 or X2 direction along the guide 61a or guide 62a by the control unit 70 and actuator 64 to the vicinity of the loading/recovery unit 10, the staining unit 40, the spinning unit 51, or the scanning unit 53.
  • the long sides of the carrier 20 parallel to the extension directions of the first conveying line 61 and the second conveying line 62, the area occupied by the first conveying line 61 and the second conveying line 62 can be reduced.
  • first conveying line 61 and the second conveying line 62 may be mounted on each of the first conveying line 61 and the second conveying line 62.
  • conveying unit 60 has been illustrated here as having separate first conveying line 61 and second conveying line 62, the conveying unit 60 may be configured so that only one line serves as both the first conveying line 61 and the second conveying line 62.
  • the slide glass 1 is transported from the dehydration section 51 to the sealing section 52 by the third transport line 63 shown in FIG. 1.
  • the slide glass 1 is also transported from the sealing section 52 to the scanning section 53 by the third transport line 63.
  • the third transport line 63 has a configuration similar to that of the second transport line 62, and transports the slide glass 1 only in the left direction (X2 direction) in FIGS. 1 and 4.
  • the sample processing system 100 is equipped with multiple transport mechanisms 30. At least one transport mechanism 30 is provided in each of the loading and recovering section 10, the staining section 40, the spinning section 51, and the scanning section 53.
  • the transport of the slide glass 1 from the loading/recovery section 10 (loading mechanism 11) to the carrier 20 of the transport section 60 (first transport line 61 or second transport line 62) and the transport from the carrier 20 of the transport section 60 (second transport line 62) to the loading/recovery section 10 (recovery mechanism 12) are performed by the transport mechanism 30 of the loading/recovery section 10.
  • the transport of the slide glass 1 from the carrier 20 of the transport section 60 (first transport line 61) to the staining section 40 and the transport from the staining section 40 to the carrier 20 of the transport section 60 (second transport line 62) are performed by the transport mechanism 30 of the staining section 40.
  • the transport of the slide glass 1 from the carrier 20 of the transport section 60 (second transport line 62) to the dehydration section 51 is performed by the transport mechanism 30 of the dehydration section 51.
  • the slide glass 1 is transported from the scanning unit 53 to the carrier 20 of the transport unit 60 (first transport line 61) by the transport mechanism 30 of the scanning unit 53.
  • the main feature of the sample processing system 100 of this embodiment is that the staining section 40 and the post-processing section 50 are arranged side by side on either side of the loading and recovery section 10. More specifically, the staining section 40, loading and recovery section 10, spinning section 51, sealing section 52, and scanning section 53 are arranged in this order in the X direction.
  • the transport mechanism 30 has a transport drive section (gripper unit) 31, a finger drive section (grip mechanism) 32, and a plurality of finger sections 33.
  • the transport drive section 31 and the finger drive section 32 are controlled by a control section 70 (see FIG. 1).
  • the transport drive unit 31 includes an X transport axis 31x, a Y transport axis 31y, and a Z transport axis 31z.
  • the X transport axis 31x allows the finger drive unit 32 and the multiple finger units 33 to move in the X1 and X2 directions.
  • the Y transport axis 31y allows the finger drive unit 32 and the multiple finger units 33 to move in the Y1 and Y2 directions.
  • the Z transport axis 31z allows the finger drive unit 32 and the multiple finger units 33 to move in the Z1 and Z2 directions. Note that at least the Z transport axis 31z is required in order for the multiple finger units 33 to grip the slide glass 1.
  • the finger drive unit 32 performs the gripping operation of the multiple finger units 33.
  • four finger units 33 are provided.
  • the slide glass 1 has a polygonal shape in plan view, and here has a rectangular shape in plan view. During transport of the slide glass 1, the two short side surfaces of the slide glass 1 are gripped by the transport mechanism 30.
  • the thickness of the slide glass 1 in the Z direction is, for example, 1.0 mm or more and 1.1 mm or less.
  • the insertion mechanism 11 and the recovery mechanism 12 are adjacent to each other in the X direction and are integrated.
  • the multiple slide glasses 1 are placed on the tray 13 so as to be adjacent to each other in the Y direction.
  • the feeding mechanism 11 can be equipped with multiple trays 13 so that the multiple trays 13 are stacked vertically (Z direction).
  • the feeding mechanism 11 also includes an identification code reader 14.
  • each of the multiple slide glasses 1 is provided with an identification code 1a.
  • the identification code reader 14 can read the information contained in the identification code 1a of each of the multiple slide glasses 1.
  • the identification code reader 14 can also be moved in the Z direction by a moving mechanism (not shown) provided in the feeding mechanism 11. Therefore, for example, when a tray 13 is introduced from outside the feeding/recovery section 10 to a predetermined layer of the feeding mechanism 11, the identification code reader 14 can move to the predetermined layer and read the information of the multiple slide glasses 1 placed on the introduced tray 13.
  • the collection mechanism 12 can accommodate multiple trays 13 so that the trays 13 are stacked vertically (Z direction).
  • the collection mechanism 12 also includes an identification code reader 24.
  • the identification code reader 24 can read the information contained in the identification code 1a of each of the multiple slide glasses 1.
  • the identification code reader 24 can also be moved in the Z direction by a moving mechanism (not shown) provided in the recovery mechanism 12. Therefore, for example, when a tray 13 transported by a moving mechanism TM2 (described below) is introduced into a specified layer of the recovery mechanism 12, the identification code reader 14 can move to the specified layer and read the information of the multiple slide glasses 1 placed on the introduced tray 13.
  • the input/collection unit 10 further includes a moving mechanism TM1 that moves the multiple trays 13 mounted on the input mechanism 11 in the Y direction individually, and a moving mechanism TM2 that moves the multiple trays 13 mounted on the collection mechanism 12 in the Y direction individually.
  • the multiple trays 13 are moved by moving the multiple placement units, on which the trays 13 are placed, in the Y direction individually by the moving mechanism TM1 or the moving mechanism TM2.
  • a container 15 may be used in which slide glasses 1 can be inserted and placed horizontally (Y direction) and stacked vertically (Z direction).
  • This container 15 is also called a basket.
  • the slide glasses 1 are removed from the container 15 and inserted into the container 15 by a dedicated transport mechanism.
  • the container 15 is provided with a bar 16 that is rotatably attached to the container 15 and prevents the slide glasses 1 from jumping out.
  • the sample processing system 100 is connected to a control unit (CPU) 70, a storage unit 80, and an operation unit 90.
  • the sample processing system 100 is connected to the control unit 70, and the control unit 70 is connected to each of the storage unit 80 and the operation unit 90.
  • the operation unit 90 may also include a display unit (monitor).
  • the control unit 70 is electrically connected to each of the loading and recovering unit 10, the multiple transport mechanisms 30, the staining unit 40, the spin-drying unit 51, the sealing unit 52, the scanning unit 53, and the transport unit 60, and controls them.
  • the control unit 70 controls processes such as the movement of the moving mechanisms TM1 and TM2 shown in FIG. 5, the movement of the identification code reader 14, the reading of the information of the identification code 1a, the movement of the finger drive unit 32 shown in FIG. 4, and the supply of reagent by the reagent supply unit.
  • the memory unit 80 stores information on the location (destination) to which the slide glass 1 should be transported, which corresponds to various information obtained by reading the identification code 1a of the slide glass 1. The operator can change the information stored in the memory unit 80 or the operation of the control unit 70 by operating the operation unit 90.
  • the second pattern is, for example, a case where a slide glass 1 that has already been stained outside the sample processing system 100 is only subjected to post-processing in the sample processing system 100.
  • the third pattern is, for example, a case where a slide glass 1 that has already been stained and dehydrated outside the sample processing system 100 is only subjected to mounting and scanning in the sample processing system 100.
  • the fourth pattern is, for example, a case where a slide glass 1 that has already been stained, dehydrated, and mounted outside the sample processing system 100 is only subjected to scanning in the sample processing system 100.
  • FIG. 8 shows a flow chart summarizing all four patterns.
  • the slide glass 1 is carried into the sample processing system 100, and the identification code 1a of each slide glass 1 is read (step S1 in FIG. 8). That is, a predetermined tray 13 on which a plurality of slide glasses 1 are placed is placed on the installation part of the insertion mechanism 11 (where the trays 13 are overlapping in FIG. 6).
  • the tray 13 is fixed to the installation part of the insertion mechanism 11 by a device such as a ball catch or a magnet catch (the position of the tray 13 shown in FIG. 1).
  • the tray 13 moves in the Y2 direction when a sensor (not shown) detects that the tray 13 has been placed in the predetermined position, or when the operator presses a predetermined open/close switch. That is, the tray 13 on which the slide glass 1 is placed moves inside the sample processing system 100 (in the Y2 direction from the position of the tray 13 shown in FIG. 1).
  • the identification code reader 14 shown in FIG. 6 reads the identification code 1a shown in FIG. 3 and FIG. 6. That is, while the multiple slide glasses 1 are moving, a reading operation is performed in which the identification code 1a of each slide glass 1 is read in sequence.
  • control unit 70 determines (judges) the destination of the slide glass 1 based on the information of the slide glass 1 obtained by the above reading operation, by referring to the destination information stored in the memory unit 80 (step S2 in Figure 8).
  • the slide glass 1 is transported to the staining section 40 or the post-processing section 50 according to the destination determined by the control section 70 (step S3 in FIG. 8).
  • step S4 in FIG. 8 one of the first to fourth patterns of processing is performed on the slide glass 1 (step S4 in FIG. 8). This completes all processing on the slide glass 1 performed within the sample processing system 100.
  • the slide glass 1 is transported from the post-processing section 50 (specifically, the scanning section 53) to the recovery mechanism 12 of the loading and recovery section 10 (step S5 in FIG. 8). Specifically, the slide glass 1 is placed on the carrier 20 of the first transport line 61 by the transport mechanism 30 of the scanning section 53, and then moved in the X1 direction by the first transport line 61 to the loading and recovery section 10. The slide glass 1 is then transported by the transport mechanism 30 of the loading and recovery section 10 and placed on the tray 13 of the recovery mechanism 12. At this time, the identification code reader 24 reads the identification code 1a on the transported slide glass 1.
  • Step S1 in Figure 9 is the same as step S1 in Figure 8.
  • control unit 70 determines whether or not the slide glass 1 requires staining processing based on the information about the slide glass 1 obtained by the reading operation (step S21 in FIG. 9).
  • the slide glass 1 is transported from the tray 13 by the transport mechanism 30 of the loading/recovery unit 10 and placed on the carrier 20 of the first transport line 61.
  • the slide glass 1 to be stained is moved in the X1 direction by the first transport line 61 and transported to the staining unit 40 by the transport mechanism 30 of the staining unit 40 (step S31 in FIG. 9).
  • the slide glass 1 is subjected to the first pattern processing, i.e., staining processing, dehydration processing, sealing processing, and scanning processing (step S41 in FIG. 9). Specifically, the sample placed on the slide glass 1 is stained on a disk in the staining unit 40. Next, the slide glass 1 is placed on the carrier 20 of the second conveyor line 62 by the conveyor mechanism 30 of the staining unit 40. Next, the slide glass 1 is moved in the X2 direction by the second conveyor line 62, and is conveyed to the dehydration unit 51 by the conveyor mechanism 30 of the dehydration unit 51. Next, the sample placed on the slide glass 1 is dehydrated in the dehydration unit 51.
  • the first pattern processing i.e., staining processing, dehydration processing, sealing processing, and scanning processing
  • the slide glass 1 is placed on the carrier 20 of the third conveyor line 63 by the conveyor mechanism 30 of the dehydration unit 51.
  • the slide glass 1 is moved in the X2 direction by the third conveyor line 63, and is subjected to sealing processing in the sealing unit 52.
  • the slide glass 1 is moved in the X2 direction by the third transport line 63 and is scanned (microscopic observation or imaging) in the scanning unit 53.
  • step S5 in FIG. 8 the slide glass 1 is transported from the scanning unit 53 to the recovery mechanism 12 of the loading and recovery unit 10 (step S5 in FIG. 9). This completes the operation of the sample processing system 100 in the first pattern.
  • control unit 70 performs an operation corresponding to step S2 of FIG. 8 by determining whether the slide glass 1 requires dehydration from the information of the slide glass 1 obtained by the reading operation (step S22 of FIG. 9).
  • the slide glass 1 is transported from the tray 13 by the transport mechanism 30 of the loading and recovering unit 10 and placed on the carrier 20 of the second transport line 62.
  • the slide glass 1 to be dehydrated is then moved in the X2 direction by the second transport line 62 and transported to the dehydration unit 51 by the transport mechanism 30 of the dehydration unit 51 (step S32 in FIG. 9).
  • the slide glass 1 is subjected to the second pattern of processing, i.e., dehydration processing, encapsulation processing, and scanning processing (step S42 in FIG. 9).
  • the sample placed on the slide glass 1 is dehydrated in the dehydration unit 51.
  • the slide glass 1 is then placed on the carrier 20 of the third transport line 63 by the transport mechanism 30 of the dehydration unit 51.
  • the slide glass 1 is then moved in the X2 direction by the third transport line 63, and is subjected to encapsulation processing in the encapsulation unit 52.
  • the slide glass 1 is then moved in the X2 direction by the third transport line 63, and is subjected to scanning processing in the scanning unit 53.
  • step S5 in FIG. 8 the slide glass 1 is transported from the scanning unit 53 to the recovery mechanism 12 of the loading and recovery unit 10 (step S5 in FIG. 9). This completes the operation of the sample processing system 100 in the second pattern.
  • control unit 70 determines whether the slide glass 1 requires mounting based on the information about the slide glass 1 obtained by the reading operation (step S23 of FIG. 9), which corresponds to step S2 of FIG. 8.
  • the slide glass 1 is transported from the tray 13 by the transport mechanism 30 of the loading/recovery unit 10 and placed on the carrier 20 of the second transport line 62.
  • the slide glass 1 is then moved in the X2 direction by the second transport line 62, transported to the dehydration unit 51 by the transport mechanism 30 of the dehydration unit 51, and then placed on the carrier 20 of the third transport line 63 by the transport mechanism 30 of the dehydration unit 51, and moved in the X2 direction by the third transport line 63 to be transported to the mounting unit 52 (step S33 in FIG. 9).
  • the slide glass 1 is subjected to a third pattern of processing, i.e., an encapsulation process and a scanning process (step S43 in FIG. 9). Specifically, the slide glass 1 to be encapsulated is subjected to an encapsulation process in the encapsulation unit 52. The slide glass 1 is then moved in the X2 direction by the third conveyor line 63, and is scanned in the scanning unit 53.
  • a third pattern of processing i.e., an encapsulation process and a scanning process.
  • step S5 in FIG. 8 the slide glass 1 is transported from the scanning unit 53 to the recovery mechanism 12 of the loading and recovery unit 10 (step S5 in FIG. 9). This completes the operation of the sample processing system 100 in the third pattern.
  • the slide glass 1 is transported from the tray 13 by the transport mechanism 30 of the loading and recovery unit 10 and placed on the carrier 20 of the second transport line 62.
  • the slide glass 1 moves in the X2 direction by the second transport line 62, is transported to the dehydration unit 51 by the transport mechanism 30 of the dehydration unit 51, is placed on the carrier 20 of the third transport line 63 by the transport mechanism 30 of the dehydration unit 51, and is moved in the X2 direction by the third transport line 63 to be transported to the scanning unit 53 (step S34 in FIG. 9).
  • the slide glass 1 is transported by the transport mechanism 30 of the loading/recovery unit 10 and placed on the carrier 20 of the second transport line 62, and then the slide glass 1 is transported near the scanning unit 53 by the second transport line 62, and the slide glass 1 may be transported into the scanning unit 53 by the transport mechanism 30 of the scanning unit 53.
  • the fourth pattern process i.e., the scanning process
  • the scanning process is performed on the slide glass 1 (step S44 in FIG. 9). Specifically, the slide glass 1 to be scanned is scanned by the scanning unit 53.
  • step S5 in FIG. 8 the slide glass 1 is transported from the scanning unit 53 to the recovery mechanism 12 of the loading and recovery unit 10 (step S5 in FIG. 9). This completes the operation of the sample processing system 100 in the fourth pattern.
  • processing of each of the four patterns can be performed automatically within the sample processing system 100.
  • each of the multiple slides fed into the sample processing system necessarily require staining, dehydration, mounting, and scanning.
  • each of the multiple slides requires one of the first to fourth patterns of processing described above, and the processing performed within the sample processing system is not uniform.
  • a sample processing system capable of performing staining, dehydration, mounting, and scanning must be designed with efficient movement lines in mind in order to perform each of the first to fourth patterns of processing.
  • a sample processing system could be constructed by arranging an input/recovery section, a staining section, a dehydration section, a mounting section, and a scanning section in the order in which the staining, dehydration, mounting, and scanning processes are carried out.
  • slides that are only to be subjected to post-processing must be transported past the staining section, resulting in a loss of time.
  • the staining section 40 and the post-processing section 50 are arranged side by side on either side of the loading and recovery section 10.
  • the flow line for transporting from the loading and recovery section 10 to the staining section 40 is separated from the flow line for transporting to the post-processing section 50.
  • the staining section 40, loading and recovering section 10, dehydration section 51, sealing section 52, and scanning section 53 are arranged in order in the X direction. This allows a slide glass 1 that requires any of the first to fourth patterns of processing to be transported and processed in a short time, allowing the sample processing system to perform processing efficiently.
  • the transport section 60 is provided with a first transport line 61 that transports the slide glass 1 in the X1 direction, and a second transport line 62 that transports the slide glass 1 in the X2 direction.
  • first transport line 61 that transports the slide glass 1 in the X1 direction
  • second transport line 62 that transports the slide glass 1 in the X2 direction.
  • the transport section 60 is linear, but the transport section 60 may be configured in an L-shape that bends at a right angle near the loading and recovery section 10 in a plan view.
  • the staining section 40, the loading and recovery section 10, and the post-processing section 50 are aligned in order along the L-shape.
  • each of the first transport line 61 and the second transport line 62 may be divided near the loading and recovery section 10 and configured as two transport lines that transport the slide glass 1 in different directions that intersect each other in a plan view.
  • the present invention can be widely used in sample processing systems.

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  • General Health & Medical Sciences (AREA)
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  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
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  • Biomedical Technology (AREA)
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PCT/JP2023/040826 2022-12-22 2023-11-13 試料処理システム Ceased WO2024135159A1 (ja)

Priority Applications (3)

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JP2024565671A JPWO2024135159A1 (https=) 2022-12-22 2023-11-13
EP23906530.3A EP4641211A1 (en) 2022-12-22 2023-11-13 Sample processing system
CN202380086546.0A CN120359402A (zh) 2022-12-22 2023-11-13 试料处理系统

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JP2001337279A (ja) * 2000-05-29 2001-12-07 Aloka Co Ltd サンプル処理装置及びカバーガラス搬送装置
JP2003014597A (ja) * 2001-07-02 2003-01-15 Seiko Epson Corp プレパラートの作成方法およびその作成装置
JP2006308575A (ja) * 2005-04-27 2006-11-09 Ventana Medical Systems Inc スライド大量自動処理システム
JP2009293983A (ja) * 2008-06-03 2009-12-17 Tabata Engineering:Kk スライド標本用染色装置
JP2011089932A (ja) * 2009-10-23 2011-05-06 Sony Corp 付着物除去装置、付着物除去方法及び生体サンプル像取得システム
JP2012141287A (ja) 2010-12-28 2012-07-26 Sakura Finetex Usa Inc 生体試料の自動処理システムと自動処理方法
JP2017211305A (ja) * 2016-05-26 2017-11-30 シスメックス株式会社 塗抹標本作製装置および塗抹標本作成方法
CN217359215U (zh) * 2021-12-15 2022-09-02 深圳市瑞图生物技术有限公司 液基制片仪

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Publication number Priority date Publication date Assignee Title
JP2001337279A (ja) * 2000-05-29 2001-12-07 Aloka Co Ltd サンプル処理装置及びカバーガラス搬送装置
JP2003014597A (ja) * 2001-07-02 2003-01-15 Seiko Epson Corp プレパラートの作成方法およびその作成装置
JP2006308575A (ja) * 2005-04-27 2006-11-09 Ventana Medical Systems Inc スライド大量自動処理システム
JP2009293983A (ja) * 2008-06-03 2009-12-17 Tabata Engineering:Kk スライド標本用染色装置
JP2011089932A (ja) * 2009-10-23 2011-05-06 Sony Corp 付着物除去装置、付着物除去方法及び生体サンプル像取得システム
JP2012141287A (ja) 2010-12-28 2012-07-26 Sakura Finetex Usa Inc 生体試料の自動処理システムと自動処理方法
JP2017211305A (ja) * 2016-05-26 2017-11-30 シスメックス株式会社 塗抹標本作製装置および塗抹標本作成方法
CN217359215U (zh) * 2021-12-15 2022-09-02 深圳市瑞图生物技术有限公司 液基制片仪

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Title
See also references of EP4641211A1

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JPWO2024135159A1 (https=) 2024-06-27
CN120359402A (zh) 2025-07-22

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